MD16G1073400.v1.1

CAAX protease self-immunity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004201
Physical Location & Seq
Reverse (-)
5148171 .. 5151334
3164 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1073400.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGGTGTACACGCTGTTCACATCTGTAGCTCAATCTTCTTTCTCCAACTTCTCAGACTCGCCCAAGCTAACTTCTTCCAGAACCCAAATCCCCTCCCTTTTGCTCGACGGCGGCAGAAGCTTCACCGCCGGCTTCTCGCCGCGCCGACCAACCCAGCTTTCAAGGCTCAAAAACGTCAGAAATACGCCCTGCATTTGCTTTTTCAGCGGCGGAGGAAAGCCCAAAGGCGATTTCCAAGAAAAGGAAAATGAGTCGGAGTGGCGGATTTTGAAGCGGTGGGATGTGCCCTGGGAATGGCAAACGGCGTCGTTAACATCGCTTGCTTGTGGACTGAGTTTTGTTTTAACAGGACTGGTAGAGGCAGCAGCTATACCGTATTTAGGAATTCGAATTCCGGAATTGAGTTTGGATGAGAAGGCAGAGATACTGTTTCTTGATCAAGGTATTACAACTGCAGCTGTGCTTGCTGTTCTATATGCTGTTGCCAGCACTTACCAACCACTGCCTGAAGATGTTTATCGCTACGTGAATGATTTGAAGGAACCTTTCAATCTACAGAAGGGATGGCTATTGTGGGCAGGGATTGGTATTGTTGGTGCCATAGCTGCTATCACGGTGACAGGGGCTGCATTGTCCTTGTTGAGGGGGGAGAGCCCAGAACGAGAGAAGGACGCTCTAGTTAGTTTGCTTCCACTGATTGGATCGTCCGGTATCAGCACTGCTTGCTTGCTAGGCATCACTGGAGTTTTGGCTCCAATTCTTGAAGAAACTGTGTTTCGCGGGTTTTTTATGGTCTCCATGACTAAGTGGGTACCTACACCAGTGGCTATTGTAATAAGTGCAGCTGTATTTGCACTTGCACATCTCACTCCTGGAGAGTTCCCCCAGCTGTTTGTGCTAGGCACTGCTCTGGGATTGTCGTATGCTCAAACTCGCAACCTTGTAACCCCCATCACAATACATGCTCTCTGGAACTCAGGAGTGATCTTGCTTCTGACCTTCCTTCAGCTACAAGGGTACGATATCAAGGAACTGTTGCAGACAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abi

Protein Analysis

350

Amino Acids

37.93

Weight (kDa)

5.83

Isoelectric Point (pI)

40.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rce1-like PF02517 241 - 326 4.1e-21 Type II CAAX prenyl endopeptidase Rce1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 811
AccB1I GGYRCC 2 cut(s) 596, 811
AccB7I CCANNNNNTGG 1 cut(s) 698
AccII CGCG 2 cut(s) 142, 780
AccIII TCCGGA 1 cut(s) 394
AciI CCGC 8 cut(s) 111, 126, 140, 207, 210, 262, 274, 780
AclWI GGATC 1 cut(s) 709
AcsI RAATTY 2 cut(s) 384, 390
AcuI CTGAAG 2 cut(s) 528, 989
AcyI GRCGYC 1 cut(s) 305
AfaI GTAC 3 cut(s) 8, 813, 1019
AfiI CCNNNNNNNGG 5 cut(s) 241, 294, 380, 642, 698
AgsI TTSAA 5 cut(s) 162, 271, 538, 550, 764
AjnI CCWGG 2 cut(s) 287, 871
AjuI GAANNNNNNNTTGG 4 cut(s) 142, 174, 215, 247
Alw26I GTCTC 1 cut(s) 799
AlwI GGATC 1 cut(s) 709
AlwNI CAGNNNCTG 1 cut(s) 626
Aor13HI TCCGGA 1 cut(s) 394
ApeKI GCWGC 6 cut(s) 362, 365, 455, 605, 626, 842
ApoI RAATTY 2 cut(s) 384, 390
Asp718I GGTACC 1 cut(s) 811
AspLEI GCGC 1 cut(s) 144
AsuHPI GGTGA 2 cut(s) 115, 628
AsuII TTCGAA 1 cut(s) 388
BaeGI GKGCMC 1 cut(s) 288
BanI GGYRCC 2 cut(s) 596, 811
BanII GRGCYC 1 cut(s) 656
BbvI GCAGC 6 cut(s) 374, 377, 467, 592, 613, 854
BccI CCATC 2 cut(s) 558, 959
BceAI ACGGC 2 cut(s) 124, 318
BciT130I CCWGG 2 cut(s) 289, 873
BclI TGATCA 1 cut(s) 436
BcoDI GTCTC 1 cut(s) 799
BfaI CTAG 3 cut(s) 677, 731, 899
BfmI CTRYAG 3 cut(s) 24, 453, 554
BisI GCNGC 9 cut(s) 112, 140, 208, 363, 366, 456, 606, 627, 843
BlsI GCNGC 9 cut(s) 113, 141, 209, 364, 367, 457, 607, 628, 844
Bme1390I CCNGG 2 cut(s) 289, 873
BmiI GGNNCC 4 cut(s) 543, 598, 753, 813
BmrFI CCNGG 2 cut(s) 289, 873
BmsI GCATC 1 cut(s) 744
BpmI CTGGAG 2 cut(s) 762, 894
Bpu14I TTCGAA 1 cut(s) 388
BsaAI YACGTR 1 cut(s) 526
BsaBI GATNNNNATC 1 cut(s) 516
BsaHI GRCGYC 1 cut(s) 305
BsaI GGTCTC 1 cut(s) 799
BsaJI CCNNGG 2 cut(s) 287, 288
BsaWI WCCGGW 2 cut(s) 394, 707
Bsc4I CCNNNNNNNGG 5 cut(s) 241, 294, 380, 642, 698
Bse118I RCCGGY 1 cut(s) 128
Bse1I ACTGG 3 cut(s) 357, 745, 821
Bse8I GATNNNNATC 1 cut(s) 516
BseAI TCCGGA 1 cut(s) 394
BseBI CCWGG 2 cut(s) 289, 873
BseDI CCNNGG 2 cut(s) 287, 288
BseGI GGATG 3 cut(s) 286, 415, 569
BseJI GATNNNNATC 1 cut(s) 516
BseLI CCNNNNNNNGG 5 cut(s) 241, 294, 380, 642, 698
BseMII CTCAG 3 cut(s) 66, 323, 990
BseNI ACTGG 3 cut(s) 357, 745, 821
BseSI GKGCMC 1 cut(s) 288
BseXI GCAGC 6 cut(s) 374, 377, 467, 592, 613, 854
BseYI CCCAGC 2 cut(s) 153, 885
BsgI GTGCAG 1 cut(s) 861
Bsh1236I CGCG 2 cut(s) 142, 780
BshNI GGYRCC 2 cut(s) 596, 811
BsiSI CCGG 3 cut(s) 129, 395, 708
BslI CCNNNNNNNGG 5 cut(s) 241, 294, 380, 642, 698
BsmAI GTCTC 1 cut(s) 799
Bso31I GGTCTC 1 cut(s) 799
Bsp119I TTCGAA 1 cut(s) 388
Bsp1286I GDGCHC 2 cut(s) 288, 656
Bsp13I TCCGGA 1 cut(s) 394
Bsp1407I TGTACA 1 cut(s) 6
Bsp143I GATC 3 cut(s) 436, 701, 984
BspACI CCGC 8 cut(s) 111, 126, 140, 207, 210, 262, 274, 780
BspCNI CTCAG 3 cut(s) 65, 324, 989
BspEI TCCGGA 1 cut(s) 394
BspFNI CGCG 2 cut(s) 142, 780
BspLI GGNNCC 4 cut(s) 543, 598, 753, 813
BspMAI CTGCAG 1 cut(s) 457
BspPI GGATC 1 cut(s) 709
BspT104I TTCGAA 1 cut(s) 388
BspT107I GGYRCC 2 cut(s) 596, 811
BspTNI GGTCTC 1 cut(s) 799
BsrFI RCCGGY 1 cut(s) 128
BsrGI TGTACA 1 cut(s) 6
BsrI ACTGG 3 cut(s) 357, 745, 821
BssAI RCCGGY 1 cut(s) 128
BssECI CCNNGG 2 cut(s) 287, 288
BssMI GATC 3 cut(s) 436, 701, 984
BssNI GRCGYC 1 cut(s) 305
Bst2UI CCWGG 2 cut(s) 289, 873
Bst4CI ACNGT 5 cut(s) 375, 429, 616, 772, 1035
BstACI GRCGYC 1 cut(s) 305
BstAPI GCANNNNNTGC 1 cut(s) 723
BstAUI TGTACA 1 cut(s) 6
BstBAI YACGTR 1 cut(s) 526
BstBI TTCGAA 1 cut(s) 388
BstC8I GCNNGC 6 cut(s) 130, 321, 465, 487, 724, 728
BstDEI CTNAG 4 cut(s) 52, 332, 804, 976
BstENI CCTNNNNNAGG 1 cut(s) 640
BstF5I GGATG 3 cut(s) 286, 415, 569
BstFNI CGCG 2 cut(s) 142, 780
BstHHI GCGC 1 cut(s) 144
BstKTI GATC 3 cut(s) 439, 704, 987
BstMAI GTCTC 1 cut(s) 799
BstMBI GATC 3 cut(s) 436, 701, 984
BstMWI GCNNNNNNNGC 9 cut(s) 117, 163, 204, 464, 605, 723, 732, 851, 895
BstNI CCWGG 2 cut(s) 289, 873
BstNSI RCATGY 1 cut(s) 965
BstSCI CCNGG 2 cut(s) 287, 871
BstSFI CTRYAG 3 cut(s) 24, 453, 554
BstSLI GKGCMC 1 cut(s) 288
BstUI CGCG 2 cut(s) 142, 780
BstV1I GCAGC 6 cut(s) 374, 377, 467, 592, 613, 854
BtgZI GCGATG 1 cut(s) 300
BtsCI GGATG 3 cut(s) 286, 415, 569
BtsI GCAGTG 3 cut(s) 500, 717, 903
BtsIMutI CAGTG 6 cut(s) 500, 692, 717, 738, 828, 903
Cac8I GCNNGC 6 cut(s) 130, 321, 465, 487, 724, 728
CaiI CAGNNNCTG 1 cut(s) 626
CfoI GCGC 1 cut(s) 144
Cfr10I RCCGGY 1 cut(s) 128
CseI GACGC 2 cut(s) 294, 680
Csp6I GTAC 3 cut(s) 7, 812, 1018
CviAII CATG 2 cut(s) 799, 962
CviQI GTAC 3 cut(s) 7, 812, 1018
DdeI CTNAG 4 cut(s) 52, 332, 804, 976
DpnI GATC 3 cut(s) 438, 703, 986
DpnII GATC 3 cut(s) 436, 701, 984
EciI GGCGGA 2 cut(s) 225, 277
Eco24I GRGCYC 1 cut(s) 656
Eco31I GGTCTC 1 cut(s) 799
Eco32I GATATC 1 cut(s) 1024
Eco57I CTGAAG 2 cut(s) 528, 989
EcoNI CCTNNNNNAGG 1 cut(s) 640
EcoRI GAATTC 2 cut(s) 384, 390
EcoRII CCWGG 2 cut(s) 287, 871
EcoRV GATATC 1 cut(s) 1024
EcoT38I GRGCYC 1 cut(s) 656
FaeI CATG 2 cut(s) 802, 965
FaiI YATR 8 cut(s) 371, 475, 477, 602, 791, 800, 924, 963
FatI CATG 2 cut(s) 798, 961
FauI CCCGC 1 cut(s) 773
FbaI TGATCA 1 cut(s) 436
Fnu4HI GCNGC 9 cut(s) 112, 140, 208, 363, 366, 456, 606, 627, 843
FokI GGATG 3 cut(s) 293, 422, 576
FriOI GRGCYC 1 cut(s) 656
Fsp4HI GCNGC 9 cut(s) 112, 140, 208, 363, 366, 456, 606, 627, 843
FspBI CTAG 3 cut(s) 677, 731, 899
GlaI GCGC 1 cut(s) 143
GluI GCNGC 9 cut(s) 112, 140, 208, 363, 366, 456, 606, 627, 843
GsaI CCCAGC 2 cut(s) 157, 889
GsuI CTGGAG 2 cut(s) 762, 894
HapII CCGG 3 cut(s) 129, 395, 708
HgaI GACGC 2 cut(s) 294, 680
HhaI GCGC 1 cut(s) 144
Hin1I GRCGYC 1 cut(s) 305
Hin1II CATG 2 cut(s) 802, 965
Hin6I GCGC 1 cut(s) 142
HinP1I GCGC 1 cut(s) 142
HincII GTYRAC 1 cut(s) 312
HindII GTYRAC 1 cut(s) 312
HindIII AAGCTT 1 cut(s) 118
HinfI GANTC 2 cut(s) 56, 251
HpaI GTTAAC 1 cut(s) 312
HpaII CCGG 3 cut(s) 129, 395, 708
HphI GGTGA 2 cut(s) 115, 628
Hpy166II GTNNAC 5 cut(s) 7, 9, 18, 312, 329
Hpy188I TCNGA 4 cut(s) 55, 179, 256, 996
Hpy188III TCNNGA 6 cut(s) 78, 395, 434, 761, 970, 978
Hpy8I GTNNAC 5 cut(s) 7, 9, 18, 312, 329
Hpy99I CGWCG 2 cut(s) 110, 310
HpyAV CCTTC 6 cut(s) 409, 532, 553, 661, 1009, 1013
HpyCH4III ACNGT 5 cut(s) 375, 429, 616, 772, 1035
HpyCH4IV ACGT 2 cut(s) 174, 525
HpyCH4V TGCA 7 cut(s) 192, 455, 629, 842, 854, 860, 1039
HpyF10VI GCNNNNNNNGC 9 cut(s) 117, 163, 204, 464, 605, 723, 732, 851, 895
HpyF3I CTNAG 4 cut(s) 52, 332, 804, 976
HpySE526I ACGT 2 cut(s) 174, 525
Hsp92I GRCGYC 1 cut(s) 305
Hsp92II CATG 2 cut(s) 802, 965
HspAI GCGC 1 cut(s) 142
Kpn2I TCCGGA 1 cut(s) 394
KpnI GGTACC 1 cut(s) 815
KroI GCCGGC 1 cut(s) 128
KroNI GCCGGC 1 cut(s) 130
Ksp22I TGATCA 1 cut(s) 436
KspAI GTTAAC 1 cut(s) 312
Kzo9I GATC 3 cut(s) 436, 701, 984
LmnI GCTCC 1 cut(s) 757
Lsp1109I GCAGC 6 cut(s) 374, 377, 467, 592, 613, 854
LweI GCATC 1 cut(s) 744
MaeI CTAG 3 cut(s) 677, 731, 899
MaeII ACGT 2 cut(s) 174, 525
MaeIII GTNAC 2 cut(s) 616, 943
MalI GATC 3 cut(s) 438, 703, 986
MboI GATC 3 cut(s) 436, 701, 984
MboII GAAGA 4 cut(s) 27, 66, 521, 776
MhlI GDGCHC 2 cut(s) 288, 656
MluCI AATT 4 cut(s) 384, 390, 398, 756
MlyI GAGTC 2 cut(s) 50, 260
MmeI TCCRAC 2 cut(s) 69, 234
MnlI CCTC 4 cut(s) 103, 206, 352, 636
MroI TCCGGA 1 cut(s) 394
MroNI GCCGGC 1 cut(s) 128
MseI TTAA 2 cut(s) 311, 344
MspA1I CMGCKG 4 cut(s) 207, 458, 845, 889
MspI CCGG 3 cut(s) 129, 395, 708
MspR9I CCNGG 2 cut(s) 289, 873
MvaI CCWGG 2 cut(s) 289, 873
MvnI CGCG 2 cut(s) 142, 780
MwoI GCNNNNNNNGC 9 cut(s) 117, 163, 204, 464, 605, 723, 732, 851, 895
NaeI GCCGGC 1 cut(s) 130
NdeII GATC 3 cut(s) 436, 701, 984
NgoMIV GCCGGC 1 cut(s) 128
NlaIII CATG 2 cut(s) 802, 965
NlaIV GGNNCC 4 cut(s) 543, 598, 753, 813
NmuCI GTSAC 1 cut(s) 616
NspI RCATGY 1 cut(s) 965
NspV TTCGAA 1 cut(s) 388
PasI CCCWGGG 1 cut(s) 288
PdiI GCCGGC 1 cut(s) 130
PflMI CCANNNNNTGG 1 cut(s) 698
PfoI TCCNGGA 1 cut(s) 871
PkrI GCNGC 9 cut(s) 113, 141, 209, 364, 367, 457, 607, 628, 844
PleI GAGTC 2 cut(s) 50, 259
PpsI GAGTC 2 cut(s) 50, 259
Ppu21I YACGTR 1 cut(s) 526
Psp6I CCWGG 2 cut(s) 287, 871
PspFI CCCAGC 2 cut(s) 153, 885
PspGI CCWGG 2 cut(s) 287, 871
PspN4I GGNNCC 4 cut(s) 543, 598, 753, 813
PsrI GAACNNNNNNTAC 1 cut(s) 31
PstI CTGCAG 1 cut(s) 457
PstNI CAGNNNCTG 1 cut(s) 626
PvuII CAGCTG 3 cut(s) 458, 845, 889
RsaI GTAC 3 cut(s) 8, 813, 1019
RsaNI GTAC 3 cut(s) 7, 812, 1018
SaqAI TTAA 2 cut(s) 311, 344
SatI GCNGC 9 cut(s) 112, 140, 208, 363, 366, 456, 606, 627, 843
Sau3AI GATC 3 cut(s) 436, 701, 984
SchI GAGTC 2 cut(s) 50, 260
ScrFI CCNGG 2 cut(s) 289, 873
SduI GDGCHC 2 cut(s) 288, 656
SfaNI GCATC 1 cut(s) 744
SfcI CTRYAG 3 cut(s) 24, 453, 554
SfuI TTCGAA 1 cut(s) 388
Sse9I AATT 4 cut(s) 384, 390, 398, 756
SsiI CCGC 8 cut(s) 111, 126, 140, 207, 210, 262, 274, 780
SspMI CTAG 3 cut(s) 677, 731, 899
StyD4I CCNGG 2 cut(s) 287, 871
TaaI ACNGT 5 cut(s) 375, 429, 616, 772, 1035
TaiI ACGT 2 cut(s) 177, 528
TaqI TCGA 2 cut(s) 105, 388
TasI AATT 4 cut(s) 384, 390, 398, 756
TatI WGTACW 1 cut(s) 6
TauI GCSGC 3 cut(s) 114, 142, 210
Tru1I TTAA 2 cut(s) 311, 344
Tru9I TTAA 2 cut(s) 311, 344
TscAI CASTG 6 cut(s) 507, 699, 724, 745, 828, 910
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 6 cut(s) 362, 365, 455, 605, 626, 842
Tsp45I GTSAC 1 cut(s) 616
TspRI CASTG 6 cut(s) 507, 699, 724, 745, 828, 910
Van91I CCANNNNNTGG 1 cut(s) 698
XagI CCTNNNNNAGG 1 cut(s) 640
XapI RAATTY 2 cut(s) 384, 390
XceI RCATGY 1 cut(s) 965
XspI CTAG 3 cut(s) 677, 731, 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.