Rroxscaffold_5G00380860

CAAX protease self-immunity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004201
Physical Location & Seq
Reverse (-)
60990301 .. 60993621
3321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00380860.1

Sequence Viewer

Length: 978 bp
ATGGTTTACACCCTCTTCACGTCTCTAGCTCAATCCCCTTTTCCCACTTTCTCACACACCCCCAACTTAAATCCCTCCAAAACCTTCCTCAAGCGAACCCACACACTTTTCCTCAATGGTCGCACCAGGTCAAACCACCTCTCACGGCTCAGAACCACCAGGCCGCCGCCTCATGTCTGCTTTTTCAGCGGCGGAGGAAAACCCAAGCCCGATTTCCAAGAAAAGGAAAATGAAGGGGGCTTGCAAATTTTGAAGAGGTGGGATGTGCCTTGGGAATGGCAAACAGTGTCATTGACATCACTGGCTTGTGGGCTGAGTTTTGTTTTGACAGGATTGGTAGAGGCAGCAGTTGTACCCTATTTAGGATTGAGCATTCAGGATTTGAGTCTAGATGAGAAGGCAGAGTTTCTGTTGGTTGCTCAAGGTGTTACGACTGCAGCTATACTTGGAGTTCTGTATACCATTGGAGAACCTTTCAGTCTACAGAGAGGGTGGCTTTTGTGGGCTGGGATCGGTCTTTTTGGTGCTGTAGCTGCTGTGGCATTGACAGGGGCTGCCACGTCCTTTTTCAGTGGGCAAACCCCAGAAAGAGAGAAGGACGCTCTAGTTAGCTTGCTCCCATTAATTGGATCATCAAGTATTAGCACTGCTTGCTTGTTAGGCATCACTGGAGTTTTGGCTCCAATTCTTGAGGAAACTGTGTTCCGCGGGTTTTTTATGGTCTCCATGACTAAGTGGGTACCTACACCAGTTGCTGTCGTAATTAGTGCAGCTGTATTTGCACTTGCACATCTCACTCCTGGAGAGTTTCCCCAGCTGTTTGTGCTAGGCGCTGCTATGGGATTGTCGTATGCTCAAACTCGCAACCTTTTGACCCCCATCACAATACATGCTTTCTGGAACTCGGGAGTTATATTGCTTCTGACCTTCCTTCAGCTTCAAGGGTACGATATCAAGGAATTGTTGCAGGCAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abi

Protein Analysis

325

Amino Acids

35.21

Weight (kDa)

7.76

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rce1-like PF02517 217 - 302 5.2e-21 Type II CAAX prenyl endopeptidase Rce1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 739
AccB1I GGYRCC 1 cut(s) 739
AccB7I CCANNNNNTGG 1 cut(s) 626
AccI GTMKAC 2 cut(s) 458, 481
AccII CGCG 1 cut(s) 708
AciI CCGC 6 cut(s) 164, 167, 189, 192, 706, 708
AclWI GGATC 2 cut(s) 518, 637
AcsI RAATTY 1 cut(s) 246
AcuI CTGAAG 1 cut(s) 917
AfaI GTAC 3 cut(s) 354, 741, 947
AfiI CCNNNNNNNGG 3 cut(s) 223, 362, 626
AgsI TTSAA 2 cut(s) 253, 941
AjiI CACGTC 2 cut(s) 21, 561
AjnI CCWGG 3 cut(s) 125, 158, 799
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AloI GAACNNNNNNTCC 2 cut(s) 686, 718
AluBI AGCT 7 cut(s) 29, 440, 533, 612, 773, 817, 937
AluI AGCT 7 cut(s) 29, 440, 533, 612, 773, 817, 937
Alw26I GTCTC 2 cut(s) 27, 727
AlwI GGATC 2 cut(s) 518, 637
AlwNI CAGNNNCTG 2 cut(s) 554, 755
Ama87I CYCGRG 1 cut(s) 904
AoxI GGCC 1 cut(s) 161
ApeKI GCWGC 6 cut(s) 344, 437, 533, 554, 770, 833
ApoI RAATTY 1 cut(s) 246
AseI ATTAAT 1 cut(s) 623
Asp718I GGTACC 1 cut(s) 739
AspLEI GCGC 1 cut(s) 833
AvaI CYCGRG 1 cut(s) 904
BanI GGYRCC 1 cut(s) 739
BbvI GCAGC 6 cut(s) 356, 449, 520, 541, 782, 820
BccI CCATC 1 cut(s) 887
BceAI ACGGC 1 cut(s) 161
BciT130I CCWGG 3 cut(s) 127, 160, 801
BcoDI GTCTC 2 cut(s) 27, 727
BfaI CTAG 4 cut(s) 26, 389, 605, 827
BfmI CTRYAG 3 cut(s) 435, 482, 528
BfoI RGCGCY 1 cut(s) 834
BisI GCNGC 9 cut(s) 164, 167, 190, 345, 438, 534, 555, 771, 834
BlsI GCNGC 9 cut(s) 165, 168, 191, 346, 439, 535, 556, 772, 835
Bme1390I CCNGG 3 cut(s) 127, 160, 801
BmeT110I CYCGRG 1 cut(s) 904
BmgBI CACGTC 2 cut(s) 21, 561
BmiI GGNNCC 2 cut(s) 681, 741
BmrFI CCNGG 3 cut(s) 127, 160, 801
BmsI GCATC 1 cut(s) 672
BpmI CTGGAG 2 cut(s) 690, 822
BpuEI CTTGAG 3 cut(s) 74, 405, 710
BsaI GGTCTC 1 cut(s) 727
BsaJI CCNNGG 2 cut(s) 269, 706
Bsc4I CCNNNNNNNGG 3 cut(s) 223, 362, 626
Bse1I ACTGG 3 cut(s) 306, 673, 749
BseBI CCWGG 3 cut(s) 127, 160, 801
BseDI CCNNGG 2 cut(s) 269, 706
BseGI GGATG 1 cut(s) 268
BseLI CCNNNNNNNGG 3 cut(s) 223, 362, 626
BseMII CTCAG 2 cut(s) 163, 305
BseNI ACTGG 3 cut(s) 306, 673, 749
BseXI GCAGC 6 cut(s) 356, 449, 520, 541, 782, 820
BseYI CCCAGC 2 cut(s) 506, 813
BsgI GTGCAG 1 cut(s) 789
Bsh1236I CGCG 1 cut(s) 708
BshFI GGCC 1 cut(s) 163
BshNI GGYRCC 1 cut(s) 739
BsiHKCI CYCGRG 1 cut(s) 904
BslI CCNNNNNNNGG 3 cut(s) 223, 362, 626
BsmAI GTCTC 2 cut(s) 27, 727
BsmBI CGTCTC 1 cut(s) 27
BsmI GAATGC 1 cut(s) 372
BsnI GGCC 1 cut(s) 163
Bso31I GGTCTC 1 cut(s) 727
BsoBI CYCGRG 1 cut(s) 904
Bsp143I GATC 2 cut(s) 510, 629
BspACI CCGC 6 cut(s) 164, 167, 189, 192, 706, 708
BspANI GGCC 1 cut(s) 163
BspCNI CTCAG 2 cut(s) 162, 306
BspFNI CGCG 1 cut(s) 708
BspLI GGNNCC 2 cut(s) 681, 741
BspMAI CTGCAG 1 cut(s) 439
BspPI GGATC 2 cut(s) 518, 637
BspT107I GGYRCC 1 cut(s) 739
BspTNI GGTCTC 1 cut(s) 727
BsrI ACTGG 3 cut(s) 306, 673, 749
BssECI CCNNGG 2 cut(s) 269, 706
BssMI GATC 2 cut(s) 510, 629
BssNAI GTATAC 1 cut(s) 459
BssT1I CCWWGG 1 cut(s) 269
Bst1107I GTATAC 1 cut(s) 459
Bst2UI CCWGG 3 cut(s) 127, 160, 801
Bst4CI ACNGT 2 cut(s) 286, 700
Bst6I CTCTTC 2 cut(s) 20, 248
BstAPI GCANNNNNTGC 1 cut(s) 651
BstC8I GCNNGC 4 cut(s) 242, 614, 652, 969
BstDEI CTNAG 3 cut(s) 149, 314, 732
BstDSI CCRYGG 1 cut(s) 706
BstF5I GGATG 1 cut(s) 268
BstFNI CGCG 1 cut(s) 708
BstH2I RGCGCY 1 cut(s) 834
BstHHI GCGC 1 cut(s) 833
BstKTI GATC 2 cut(s) 513, 632
BstMAI GTCTC 2 cut(s) 27, 727
BstMBI GATC 2 cut(s) 510, 629
BstMWI GCNNNNNNNGC 7 cut(s) 186, 533, 539, 651, 660, 779, 823
BstNI CCWGG 3 cut(s) 127, 160, 801
BstNSI RCATGY 1 cut(s) 893
BstSCI CCNGG 3 cut(s) 125, 158, 799
BstSFI CTRYAG 3 cut(s) 435, 482, 528
BstUI CGCG 1 cut(s) 708
BstV1I GCAGC 6 cut(s) 356, 449, 520, 541, 782, 820
BstZ17I GTATAC 1 cut(s) 459
BsuRI GGCC 1 cut(s) 163
BtgI CCRYGG 1 cut(s) 706
BtrI CACGTC 2 cut(s) 21, 561
BtsCI GGATG 1 cut(s) 268
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 5 cut(s) 291, 299, 577, 645, 666
Cac8I GCNNGC 4 cut(s) 242, 614, 652, 969
CaiI CAGNNNCTG 2 cut(s) 554, 755
CfoI GCGC 1 cut(s) 833
Cfr42I CCGCGG 1 cut(s) 709
CseI GACGC 1 cut(s) 608
CsiI ACCWGGT 1 cut(s) 125
Csp6I GTAC 3 cut(s) 353, 740, 946
CviAII CATG 3 cut(s) 173, 727, 890
CviQI GTAC 3 cut(s) 353, 740, 946
DdeI CTNAG 3 cut(s) 149, 314, 732
DpnI GATC 2 cut(s) 512, 631
DpnII GATC 2 cut(s) 510, 629
Eam1104I CTCTTC 2 cut(s) 20, 248
EarI CTCTTC 2 cut(s) 20, 248
EciI GGCGGA 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 269
Eco31I GGTCTC 1 cut(s) 727
Eco32I GATATC 1 cut(s) 952
Eco57I CTGAAG 1 cut(s) 917
Eco88I CYCGRG 1 cut(s) 904
EcoRII CCWGG 3 cut(s) 125, 158, 799
EcoRV GATATC 1 cut(s) 952
EcoT14I CCWWGG 1 cut(s) 269
ErhI CCWWGG 1 cut(s) 269
Esp3I CGTCTC 1 cut(s) 27
FaeI CATG 3 cut(s) 176, 730, 893
FaiI YATR 9 cut(s) 174, 443, 459, 719, 728, 839, 852, 891, 914
FatI CATG 3 cut(s) 172, 726, 889
FauI CCCGC 1 cut(s) 701
FblI GTMKAC 2 cut(s) 458, 481
Fnu4HI GCNGC 9 cut(s) 164, 167, 190, 345, 438, 534, 555, 771, 834
FokI GGATG 1 cut(s) 275
Fsp4HI GCNGC 9 cut(s) 164, 167, 190, 345, 438, 534, 555, 771, 834
FspBI CTAG 4 cut(s) 26, 389, 605, 827
GlaI GCGC 1 cut(s) 832
GluI GCNGC 9 cut(s) 164, 167, 190, 345, 438, 534, 555, 771, 834
GsaI CCCAGC 2 cut(s) 510, 817
GsuI CTGGAG 2 cut(s) 690, 822
HaeII RGCGCY 1 cut(s) 834
HaeIII GGCC 1 cut(s) 163
HgaI GACGC 1 cut(s) 608
HhaI GCGC 1 cut(s) 833
Hin1II CATG 3 cut(s) 176, 730, 893
Hin6I GCGC 1 cut(s) 831
HinP1I GCGC 1 cut(s) 831
HinfI GANTC 1 cut(s) 385
Hpy166II GTNNAC 3 cut(s) 7, 459, 482
Hpy188I TCNGA 2 cut(s) 152, 924
Hpy188III TCNNGA 5 cut(s) 377, 389, 689, 898, 906
Hpy8I GTNNAC 3 cut(s) 7, 459, 482
HpyAV CCTTC 6 cut(s) 94, 227, 391, 589, 937, 941
HpyCH4III ACNGT 2 cut(s) 286, 700
HpyCH4IV ACGT 2 cut(s) 20, 560
HpyCH4V TGCA 6 cut(s) 244, 437, 770, 782, 788, 967
HpyF10VI GCNNNNNNNGC 7 cut(s) 186, 533, 539, 651, 660, 779, 823
HpyF3I CTNAG 3 cut(s) 149, 314, 732
HpySE526I ACGT 2 cut(s) 20, 560
Hsp92II CATG 3 cut(s) 176, 730, 893
HspAI GCGC 1 cut(s) 831
KpnI GGTACC 1 cut(s) 743
KspI CCGCGG 1 cut(s) 709
Kzo9I GATC 2 cut(s) 510, 629
LmnI GCTCC 2 cut(s) 621, 685
Lsp1109I GCAGC 6 cut(s) 356, 449, 520, 541, 782, 820
LweI GCATC 1 cut(s) 672
MabI ACCWGGT 1 cut(s) 125
MaeI CTAG 4 cut(s) 26, 389, 605, 827
MaeII ACGT 2 cut(s) 20, 560
MaeIII GTNAC 1 cut(s) 427
MalI GATC 2 cut(s) 512, 631
MboI GATC 2 cut(s) 510, 629
MboII GAAGA 2 cut(s) 7, 265
MluCI AATT 5 cut(s) 246, 624, 684, 762, 959
MlyI GAGTC 1 cut(s) 394
MseI TTAA 2 cut(s) 68, 623
MspA1I CMGCKG 4 cut(s) 189, 708, 773, 817
MspR9I CCNGG 3 cut(s) 127, 160, 801
Mva1269I GAATGC 1 cut(s) 372
MvaI CCWGG 3 cut(s) 127, 160, 801
MvnI CGCG 1 cut(s) 708
MwoI GCNNNNNNNGC 7 cut(s) 186, 533, 539, 651, 660, 779, 823
NdeII GATC 2 cut(s) 510, 629
NlaIII CATG 3 cut(s) 176, 730, 893
NlaIV GGNNCC 2 cut(s) 681, 741
NspI RCATGY 1 cut(s) 893
PctI GAATGC 1 cut(s) 372
PflMI CCANNNNNTGG 1 cut(s) 626
PfoI TCCNGGA 1 cut(s) 799
PkrI GCNGC 9 cut(s) 165, 168, 191, 346, 439, 535, 556, 772, 835
PleI GAGTC 1 cut(s) 393
PpsI GAGTC 1 cut(s) 393
PshBI ATTAAT 1 cut(s) 623
Psp6I CCWGG 3 cut(s) 125, 158, 799
PspFI CCCAGC 2 cut(s) 506, 813
PspGI CCWGG 3 cut(s) 125, 158, 799
PspN4I GGNNCC 2 cut(s) 681, 741
PstI CTGCAG 1 cut(s) 439
PstNI CAGNNNCTG 2 cut(s) 554, 755
PvuII CAGCTG 2 cut(s) 773, 817
RsaI GTAC 3 cut(s) 354, 741, 947
RsaNI GTAC 3 cut(s) 353, 740, 946
SacII CCGCGG 1 cut(s) 709
SaqAI TTAA 2 cut(s) 68, 623
SatI GCNGC 9 cut(s) 164, 167, 190, 345, 438, 534, 555, 771, 834
Sau3AI GATC 2 cut(s) 510, 629
SchI GAGTC 1 cut(s) 394
ScrFI CCNGG 3 cut(s) 127, 160, 801
SexAI ACCWGGT 1 cut(s) 125
SfaNI GCATC 1 cut(s) 672
SfcI CTRYAG 3 cut(s) 435, 482, 528
Sfr303I CCGCGG 1 cut(s) 709
SgrBI CCGCGG 1 cut(s) 709
SmlI CTYRAG 3 cut(s) 89, 420, 689
SmoI CTYRAG 3 cut(s) 89, 420, 689
Sse9I AATT 5 cut(s) 246, 624, 684, 762, 959
SsiI CCGC 6 cut(s) 164, 167, 189, 192, 706, 708
SspMI CTAG 4 cut(s) 26, 389, 605, 827
StyD4I CCNGG 3 cut(s) 125, 158, 799
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 2 cut(s) 286, 700
TaiI ACGT 2 cut(s) 23, 563
TaqII GACCGA 1 cut(s) 503
TasI AATT 5 cut(s) 246, 624, 684, 762, 959
TauI GCSGC 3 cut(s) 166, 169, 192
Tru1I TTAA 2 cut(s) 68, 623
Tru9I TTAA 2 cut(s) 68, 623
TscAI CASTG 5 cut(s) 291, 306, 577, 652, 673
TseI GCWGC 6 cut(s) 344, 437, 533, 554, 770, 833
TspDTI ATGAA 1 cut(s) 246
TspRI CASTG 5 cut(s) 291, 306, 577, 652, 673
Van91I CCANNNNNTGG 1 cut(s) 626
VspI ATTAAT 1 cut(s) 623
XapI RAATTY 1 cut(s) 246
XbaI TCTAGA 1 cut(s) 388
XceI RCATGY 1 cut(s) 893
XmiI GTMKAC 2 cut(s) 458, 481
XspI CTAG 4 cut(s) 26, 389, 605, 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.