Rh4BG385600

CAAX protease self-immunity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004201
Physical Location & Seq
Reverse (-)
53990664 .. 54000784
10121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG385600.1

Sequence Viewer

Length: 1182 bp
ATGGTTTACACCCTCTTCACATCTCTAGTTCAATCCCCCCTTCCCACTTTCTCACACACCCACAACTTAAATCCCTCCAAAACCTTCCTCAAGCGAACCCACACACTTTTCCTCAATGGTCACACCAGGTCAAACCACCTCTCACGGCTCAGAACCACCAGGCCGCCGCCTCATGTCTGCTTTTCCAGCGGCGGAGGAAAACCCAGACCCGATTTCCAAGAAAAGGAAAATGAAGGGGGCTTGCAAATTTTGAAGAGGTGGGATGTGCCTTGGGAATGGCAAACAGTGTCATTGACATCACTGGCTTGTGGGCTGAGTTTTGTTTTGACAGGATTGGTAGAGGCAGCAGTTGTACCCTATTTAGGATTGAGCATTCAGGATTTGAGTCTAGATGAGAAGGCAGAGTTTCTGTTGGTTGCTCAAGGTGTTACGACTGCAGCTATACTTGGAGTTCTGTATACGGTTGCCGGAAAATTCCAACCGCTACCTCAAGATATTTATCGCTATGATTGGAGGGAACCTTTCAGTCTACAGAGAGGGTGGCTTTTGTGGGCTGGGATTGGTCTTGTTGGTGCTGTAGCTGCTGTGGCATTGACAGGGGCTGCCACGTCCTTTTTCAGTGGGCAAACCCCAGAAAGAGAGAAGGACGCTCTAGTTAGCTTGCTCCCATTAATTGGATCATCAAGTATTAGCACTGCTTGCTTGTTAGGCATCACTGGAGTTTTGGCTCCAATTCTTGAGGAAACTGTGTTCCGCGGGTTTTTTATGGTCTCCATGACTAAGTGGGTACCTACACCAGTTGCTGTCGTAATTAGTGCAGCTGTATTTGCACTTGCACATCTCACTCCTGGAGAGTTTCCCCAGCTGTTTGTGCTAGGCGCTGCTATGGGATTGTCGTATGCTCAAACTCGCAACCTTTTGACCCCCATCACAATACATGCTTTCTGGAACTCGGGAGTTATATTGCTTCTGACCTTCCTTCAGTGCCTGACCACACTGCTAAATTTCAAGTCACCAGAAAAGGCGGCTGATCCTTCGGGGGCAGACCCCTCTTCAGGAAGCATATATTGGCTACAAATTAAGAGCAGACCCTCACCGTCAACTTGGGCTAATAATCTTGCTTTCACTCCACCTCTCTTCTCTGTACTTCTGTGTGTTCCTCTTGTTTTCTTAGCTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abi

Protein Analysis

393

Amino Acids

42.71

Weight (kDa)

8.57

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rce1-like PF02517 233 - 318 7.4e-21 Type II CAAX prenyl endopeptidase Rce1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 787
AccB1I GGYRCC 1 cut(s) 787
AccB7I CCANNNNNTGG 1 cut(s) 674
AccI GTMKAC 2 cut(s) 458, 529
AccII CGCG 1 cut(s) 756
AciI CCGC 8 cut(s) 164, 167, 189, 192, 482, 754, 756, 1025
AclWI GGATC 2 cut(s) 685, 1025
AcsI RAATTY 3 cut(s) 246, 473, 1003
AcuI CTGAAG 2 cut(s) 965, 1038
AfaI GTAC 3 cut(s) 354, 789, 1146
AfiI CCNNNNNNNGG 4 cut(s) 223, 362, 674, 1055
AgsI TTSAA 3 cut(s) 32, 253, 1009
AjiI CACGTC 1 cut(s) 609
AjnI CCWGG 3 cut(s) 125, 158, 847
AjuI GAANNNNNNNTTGG 2 cut(s) 1051, 1083
AloI GAACNNNNNNTCC 2 cut(s) 734, 766
AluBI AGCT 6 cut(s) 440, 581, 660, 821, 865, 1175
AluI AGCT 6 cut(s) 440, 581, 660, 821, 865, 1175
Alw26I GTCTC 1 cut(s) 775
AlwI GGATC 2 cut(s) 685, 1025
AlwNI CAGNNNCTG 3 cut(s) 602, 803, 988
Ama87I CYCGRG 1 cut(s) 952
AoxI GGCC 1 cut(s) 161
ApeKI GCWGC 6 cut(s) 344, 437, 581, 602, 818, 881
ApoI RAATTY 3 cut(s) 246, 473, 1003
AseI ATTAAT 1 cut(s) 671
Asp718I GGTACC 1 cut(s) 787
AspLEI GCGC 1 cut(s) 881
AsuHPI GGTGA 2 cut(s) 1005, 1086
AvaI CYCGRG 1 cut(s) 952
BanI GGYRCC 1 cut(s) 787
BbvI GCAGC 6 cut(s) 356, 449, 568, 589, 830, 868
BccI CCATC 1 cut(s) 935
BceAI ACGGC 1 cut(s) 161
BciT130I CCWGG 3 cut(s) 127, 160, 849
BcoDI GTCTC 1 cut(s) 775
BfaI CTAG 5 cut(s) 26, 389, 653, 875, 1180
BfmI CTRYAG 3 cut(s) 435, 530, 576
BfoI RGCGCY 1 cut(s) 882
Bme1390I CCNGG 3 cut(s) 127, 160, 849
BmeT110I CYCGRG 1 cut(s) 952
BmgBI CACGTC 1 cut(s) 609
BmiI GGNNCC 3 cut(s) 519, 729, 789
BmrFI CCNGG 3 cut(s) 127, 160, 849
BmsI GCATC 1 cut(s) 720
BpmI CTGGAG 2 cut(s) 738, 870
BpuEI CTTGAG 4 cut(s) 74, 405, 474, 758
BsaBI GATNNNNATC 1 cut(s) 498
BsaI GGTCTC 1 cut(s) 775
BsaJI CCNNGG 2 cut(s) 269, 754
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 362, 674, 1055
Bse1I ACTGG 3 cut(s) 306, 721, 797
Bse8I GATNNNNATC 1 cut(s) 498
BseBI CCWGG 3 cut(s) 127, 160, 849
BseDI CCNNGG 2 cut(s) 269, 754
BseGI GGATG 1 cut(s) 268
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 4 cut(s) 223, 362, 674, 1055
BseMII CTCAG 2 cut(s) 163, 305
BseNI ACTGG 3 cut(s) 306, 721, 797
BseXI GCAGC 6 cut(s) 356, 449, 568, 589, 830, 868
BseYI CCCAGC 2 cut(s) 554, 861
BsgI GTGCAG 1 cut(s) 837
Bsh1236I CGCG 1 cut(s) 756
BshFI GGCC 1 cut(s) 163
BshNI GGYRCC 1 cut(s) 787
BsiHKCI CYCGRG 1 cut(s) 952
BsiSI CCGG 1 cut(s) 468
BslI CCNNNNNNNGG 4 cut(s) 223, 362, 674, 1055
BsmAI GTCTC 1 cut(s) 775
BsmI GAATGC 1 cut(s) 372
BsnI GGCC 1 cut(s) 163
Bso31I GGTCTC 1 cut(s) 775
BsoBI CYCGRG 1 cut(s) 952
Bsp143I GATC 2 cut(s) 677, 1030
BspACI CCGC 8 cut(s) 164, 167, 189, 192, 482, 754, 756, 1025
BspANI GGCC 1 cut(s) 163
BspCNI CTCAG 2 cut(s) 162, 306
BspFNI CGCG 1 cut(s) 756
BspLI GGNNCC 3 cut(s) 519, 729, 789
BspMAI CTGCAG 1 cut(s) 439
BspPI GGATC 2 cut(s) 685, 1025
BspT107I GGYRCC 1 cut(s) 787
BspTNI GGTCTC 1 cut(s) 775
BsrI ACTGG 3 cut(s) 306, 721, 797
BssECI CCNNGG 2 cut(s) 269, 754
BssMI GATC 2 cut(s) 677, 1030
BssNAI GTATAC 1 cut(s) 459
BssT1I CCWWGG 1 cut(s) 269
Bst1107I GTATAC 1 cut(s) 459
Bst2UI CCWGG 3 cut(s) 127, 160, 849
Bst4CI ACNGT 4 cut(s) 286, 463, 748, 1098
Bst6I CTCTTC 4 cut(s) 20, 248, 1057, 1142
BstAPI GCANNNNNTGC 1 cut(s) 699
BstC8I GCNNGC 3 cut(s) 242, 662, 700
BstDEI CTNAG 4 cut(s) 149, 314, 780, 1171
BstDSI CCRYGG 1 cut(s) 754
BstF5I GGATG 1 cut(s) 268
BstFNI CGCG 1 cut(s) 756
BstH2I RGCGCY 1 cut(s) 882
BstHHI GCGC 1 cut(s) 881
BstKTI GATC 2 cut(s) 680, 1033
BstMAI GTCTC 1 cut(s) 775
BstMBI GATC 2 cut(s) 677, 1030
BstMWI GCNNNNNNNGC 7 cut(s) 186, 581, 587, 699, 708, 827, 871
BstNI CCWGG 3 cut(s) 127, 160, 849
BstNSI RCATGY 1 cut(s) 941
BstSCI CCNGG 3 cut(s) 125, 158, 847
BstSFI CTRYAG 3 cut(s) 435, 530, 576
BstUI CGCG 1 cut(s) 756
BstV1I GCAGC 6 cut(s) 356, 449, 568, 589, 830, 868
BstZ17I GTATAC 1 cut(s) 459
BsuRI GGCC 1 cut(s) 163
BtgI CCRYGG 1 cut(s) 754
BtrI CACGTC 1 cut(s) 609
BtsCI GGATG 1 cut(s) 268
BtsI GCAGTG 2 cut(s) 693, 995
BtsIMutI CAGTG 7 cut(s) 291, 299, 625, 693, 714, 989, 995
Cac8I GCNNGC 3 cut(s) 242, 662, 700
CaiI CAGNNNCTG 3 cut(s) 602, 803, 988
CfoI GCGC 1 cut(s) 881
Cfr42I CCGCGG 1 cut(s) 757
CseI GACGC 1 cut(s) 656
CsiI ACCWGGT 1 cut(s) 125
Csp6I GTAC 3 cut(s) 353, 788, 1145
CviAII CATG 3 cut(s) 173, 775, 938
CviQI GTAC 3 cut(s) 353, 788, 1145
DdeI CTNAG 4 cut(s) 149, 314, 780, 1171
DpnI GATC 2 cut(s) 679, 1032
DpnII GATC 2 cut(s) 677, 1030
Eam1104I CTCTTC 4 cut(s) 20, 248, 1057, 1142
EarI CTCTTC 4 cut(s) 20, 248, 1057, 1142
EciI GGCGGA 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 269
Eco31I GGTCTC 1 cut(s) 775
Eco57I CTGAAG 2 cut(s) 965, 1038
Eco88I CYCGRG 1 cut(s) 952
EcoRII CCWGG 3 cut(s) 125, 158, 847
EcoT14I CCWWGG 1 cut(s) 269
ErhI CCWWGG 1 cut(s) 269
FaeI CATG 3 cut(s) 176, 778, 941
FatI CATG 3 cut(s) 172, 774, 937
FauI CCCGC 1 cut(s) 749
FblI GTMKAC 2 cut(s) 458, 529
FokI GGATG 1 cut(s) 275
FspBI CTAG 5 cut(s) 26, 389, 653, 875, 1180
GlaI GCGC 1 cut(s) 880
GsaI CCCAGC 2 cut(s) 558, 865
GsuI CTGGAG 2 cut(s) 738, 870
HaeII RGCGCY 1 cut(s) 882
HaeIII GGCC 1 cut(s) 163
HapII CCGG 1 cut(s) 468
HgaI GACGC 1 cut(s) 656
HhaI GCGC 1 cut(s) 881
Hin1II CATG 3 cut(s) 176, 778, 941
Hin6I GCGC 1 cut(s) 879
HinP1I GCGC 1 cut(s) 879
HincII GTYRAC 1 cut(s) 1101
HindII GTYRAC 1 cut(s) 1101
HinfI GANTC 1 cut(s) 385
HpaII CCGG 1 cut(s) 468
HphI GGTGA 2 cut(s) 1005, 1086
Hpy166II GTNNAC 4 cut(s) 7, 459, 530, 1101
Hpy188I TCNGA 2 cut(s) 152, 972
Hpy188III TCNNGA 7 cut(s) 377, 389, 491, 737, 946, 954, 1056
Hpy8I GTNNAC 4 cut(s) 7, 459, 530, 1101
HpyAV CCTTC 8 cut(s) 50, 94, 227, 391, 637, 985, 989, 1044
HpyCH4III ACNGT 4 cut(s) 286, 463, 748, 1098
HpyCH4IV ACGT 1 cut(s) 608
HpyCH4V TGCA 5 cut(s) 244, 437, 818, 830, 836
HpyF10VI GCNNNNNNNGC 7 cut(s) 186, 581, 587, 699, 708, 827, 871
HpyF3I CTNAG 4 cut(s) 149, 314, 780, 1171
HpySE526I ACGT 1 cut(s) 608
Hsp92II CATG 3 cut(s) 176, 778, 941
HspAI GCGC 1 cut(s) 879
KpnI GGTACC 1 cut(s) 791
KspI CCGCGG 1 cut(s) 757
Kzo9I GATC 2 cut(s) 677, 1030
LmnI GCTCC 2 cut(s) 669, 733
Lsp1109I GCAGC 6 cut(s) 356, 449, 568, 589, 830, 868
LweI GCATC 1 cut(s) 720
MabI ACCWGGT 1 cut(s) 125
MaeI CTAG 5 cut(s) 26, 389, 653, 875, 1180
MaeII ACGT 1 cut(s) 608
MaeIII GTNAC 3 cut(s) 119, 427, 1011
MalI GATC 2 cut(s) 679, 1032
MboI GATC 2 cut(s) 677, 1030
MboII GAAGA 4 cut(s) 7, 265, 1044, 1129
MluCI AATT 7 cut(s) 246, 473, 672, 732, 810, 1003, 1077
MlyI GAGTC 1 cut(s) 394
MmeI TCCRAC 1 cut(s) 502
MseI TTAA 3 cut(s) 68, 671, 1080
MspA1I CMGCKG 4 cut(s) 189, 756, 821, 865
MspI CCGG 1 cut(s) 468
MspR9I CCNGG 3 cut(s) 127, 160, 849
Mva1269I GAATGC 1 cut(s) 372
MvaI CCWGG 3 cut(s) 127, 160, 849
MvnI CGCG 1 cut(s) 756
MwoI GCNNNNNNNGC 7 cut(s) 186, 581, 587, 699, 708, 827, 871
NdeII GATC 2 cut(s) 677, 1030
NlaIII CATG 3 cut(s) 176, 778, 941
NlaIV GGNNCC 3 cut(s) 519, 729, 789
NmuCI GTSAC 2 cut(s) 119, 1011
NspI RCATGY 1 cut(s) 941
PctI GAATGC 1 cut(s) 372
PflMI CCANNNNNTGG 1 cut(s) 674
PfoI TCCNGGA 1 cut(s) 847
PleI GAGTC 1 cut(s) 393
PpsI GAGTC 1 cut(s) 393
PshBI ATTAAT 1 cut(s) 671
Psp6I CCWGG 3 cut(s) 125, 158, 847
PspFI CCCAGC 2 cut(s) 554, 861
PspGI CCWGG 3 cut(s) 125, 158, 847
PspN4I GGNNCC 3 cut(s) 519, 729, 789
PstI CTGCAG 1 cut(s) 439
PstNI CAGNNNCTG 3 cut(s) 602, 803, 988
PvuII CAGCTG 2 cut(s) 821, 865
RsaI GTAC 3 cut(s) 354, 789, 1146
RsaNI GTAC 3 cut(s) 353, 788, 1145
SacII CCGCGG 1 cut(s) 757
SaqAI TTAA 3 cut(s) 68, 671, 1080
Sau3AI GATC 2 cut(s) 677, 1030
SchI GAGTC 1 cut(s) 394
ScrFI CCNGG 3 cut(s) 127, 160, 849
SexAI ACCWGGT 1 cut(s) 125
SfaNI GCATC 1 cut(s) 720
SfcI CTRYAG 3 cut(s) 435, 530, 576
Sfr303I CCGCGG 1 cut(s) 757
SgrBI CCGCGG 1 cut(s) 757
SmlI CTYRAG 4 cut(s) 89, 420, 489, 737
SmoI CTYRAG 4 cut(s) 89, 420, 489, 737
Sse9I AATT 7 cut(s) 246, 473, 672, 732, 810, 1003, 1077
SsiI CCGC 8 cut(s) 164, 167, 189, 192, 482, 754, 756, 1025
SspMI CTAG 5 cut(s) 26, 389, 653, 875, 1180
StyD4I CCNGG 3 cut(s) 125, 158, 847
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 4 cut(s) 286, 463, 748, 1098
TaiI ACGT 1 cut(s) 611
TasI AATT 7 cut(s) 246, 473, 672, 732, 810, 1003, 1077
TatI WGTACW 1 cut(s) 1144
TauI GCSGC 4 cut(s) 166, 169, 192, 1028
Tru1I TTAA 3 cut(s) 68, 671, 1080
Tru9I TTAA 3 cut(s) 68, 671, 1080
TscAI CASTG 7 cut(s) 291, 306, 625, 700, 721, 989, 1002
TseFI GTSAC 2 cut(s) 119, 1011
TseI GCWGC 6 cut(s) 344, 437, 581, 602, 818, 881
Tsp45I GTSAC 2 cut(s) 119, 1011
TspDTI ATGAA 1 cut(s) 246
TspRI CASTG 7 cut(s) 291, 306, 625, 700, 721, 989, 1002
Van91I CCANNNNNTGG 1 cut(s) 674
VspI ATTAAT 1 cut(s) 671
XapI RAATTY 3 cut(s) 246, 473, 1003
XbaI TCTAGA 1 cut(s) 388
XceI RCATGY 1 cut(s) 941
XmiI GTMKAC 2 cut(s) 458, 529
XspI CTAG 5 cut(s) 26, 389, 653, 875, 1180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.