FvH4_4g36370

Myo-inositol oxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001948
Physical Location & Seq
Forward (+)
33327365 .. 33330405
3041 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36370.t2

Sequence Viewer

Length: 690 bp
ATGAGAGAAGAATATGGAAAGTTGGACAAGGTGGAAATGAGCATATGGGAATGCTGTGAACTTTTGAATGAAGTTGTGGATGAGAGTGATCCTGATTTAGATGAGCCTCAGATTGAGCATCTGCTGCAGACTGCTGAAGCTGTCAGAAAAGACTACCCTAAAGAAGGCTGGCTACATTTAACTGCCCTGATCCATGATCTTGGAAAGGTGCTTAATCTTCCTGCTTTTGGTGAACTTCCTCAGTGGGCTGTTGTAGGAGACACATTTCCTGTTGGCTGTGCCTTTGATGGGTCAATCGTTCATCACAAGCATTTCCAAGGGAATCCTGACTACAGCAATCCTGCTTATAACACTGAATATGGAATTTACTCTGAAGGCTGCGGACTTAACAATGTCCTGATGTCATGGGGACACGATGACTACATGTACTTGGTGGCCAAGGGGAATAACTCTACTCTTCCTTCAGCTGGTCTTTTCATCATCAGATACCATTCATTTTACGCTTTACACAGGGCAGGAGCATATAAGCACTTGATGAATGAAGAGGACATTGAGAATTTGAAGTGGCTCAAAGTATTCAACAAGTATGACCTTTACAGTAAGAGCAAAGTTCGGATTGATGTTGAGAAAGTCAAGCCCTACTATCTCTCACTAATTGAGAAGTACTTCCCTGCAAAACTGAGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko00562 map00053 map00562
Pfam Domains
Protein Families
MIOX

Protein Analysis

230

Amino Acids

26.5

Weight (kDa)

5.34

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIOX PF05153 3 - 234 2.7e-117 Myo-inositol oxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 348
AciI CCGC 1 cut(s) 381
AclWI GGATC 2 cut(s) 83, 184
AcoI YGGCCR 1 cut(s) 435
AcsI RAATTY 2 cut(s) 363, 556
AcuI CTGAAG 3 cut(s) 156, 393, 447
AfaI GTAC 2 cut(s) 428, 665
AfiI CCNNNNNNNGG 4 cut(s) 164, 227, 288, 467
AflIII ACRYGT 1 cut(s) 423
AgsI TTSAA 3 cut(s) 67, 562, 580
AluBI AGCT 2 cut(s) 140, 467
AluI AGCT 2 cut(s) 140, 467
Alw26I GTCTC 1 cut(s) 252
AlwI GGATC 2 cut(s) 83, 184
AoxI GGCC 1 cut(s) 435
ApeKI GCWGC 2 cut(s) 124, 378
ApoI RAATTY 2 cut(s) 363, 556
Asp700I GAANNNNTTC 1 cut(s) 665
AsuHPI GGTGA 1 cut(s) 242
BalI TGGCCA 1 cut(s) 437
BarI GAAGNNNNNNTAC 2 cut(s) 156, 188
BbvI GCAGC 2 cut(s) 111, 365
BccI CCATC 2 cut(s) 281, 678
BcoDI GTCTC 1 cut(s) 252
BfmI CTRYAG 2 cut(s) 125, 331
BisI GCNGC 2 cut(s) 125, 379
BlsI GCNGC 2 cut(s) 126, 380
BmcAI AGTACT 1 cut(s) 665
BmsI GCATC 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 316, 438
Bsc4I CCNNNNNNNGG 4 cut(s) 164, 227, 288, 467
BseDI CCNNGG 2 cut(s) 316, 438
BseGI GGATG 1 cut(s) 85
BseLI CCNNNNNNNGG 4 cut(s) 164, 227, 288, 467
BseMII CTCAG 3 cut(s) 122, 254, 671
BseXI GCAGC 2 cut(s) 111, 365
BshFI GGCC 1 cut(s) 437
BslFI GGGAC 1 cut(s) 423
BslI CCNNNNNNNGG 4 cut(s) 164, 227, 288, 467
BsmAI GTCTC 1 cut(s) 252
BsmFI GGGAC 1 cut(s) 423
BsmI GAATGC 1 cut(s) 56
BsnI GGCC 1 cut(s) 437
Bsp143I GATC 3 cut(s) 88, 189, 196
BspACI CCGC 1 cut(s) 381
BspANI GGCC 1 cut(s) 437
BspCNI CTCAG 3 cut(s) 121, 253, 672
BspMAI CTGCAG 1 cut(s) 129
BspPI GGATC 2 cut(s) 83, 184
BssECI CCNNGG 2 cut(s) 316, 438
BssMI GATC 3 cut(s) 88, 189, 196
BssT1I CCWWGG 2 cut(s) 316, 438
Bst4CI ACNGT 1 cut(s) 599
Bst6I CTCTTC 2 cut(s) 462, 537
BstAPI GCANNNNNTGC 1 cut(s) 124
BstC8I GCNNGC 1 cut(s) 170
BstDEI CTNAG 3 cut(s) 108, 240, 680
BstENI CCTNNNNNAGG 1 cut(s) 162
BstF5I GGATG 1 cut(s) 85
BstKTI GATC 3 cut(s) 91, 192, 199
BstMAI GTCTC 1 cut(s) 252
BstMBI GATC 3 cut(s) 88, 189, 196
BstMWI GCNNNNNNNGC 1 cut(s) 124
BstNSI RCATGY 1 cut(s) 427
BstSFI CTRYAG 2 cut(s) 125, 331
BstV1I GCAGC 2 cut(s) 111, 365
BstXI CCANNNNNNTGG 1 cut(s) 200
BsuRI GGCC 1 cut(s) 437
BtsCI GGATG 1 cut(s) 85
BtsIMutI CAGTG 2 cut(s) 248, 351
Cac8I GCNNGC 1 cut(s) 170
Csp6I GTAC 2 cut(s) 427, 664
CviAII CATG 3 cut(s) 194, 405, 424
CviQI GTAC 2 cut(s) 427, 664
DdeI CTNAG 3 cut(s) 108, 240, 680
DpnI GATC 3 cut(s) 90, 191, 198
DpnII GATC 3 cut(s) 88, 189, 196
EaeI YGGCCR 1 cut(s) 435
Eam1104I CTCTTC 2 cut(s) 462, 537
EarI CTCTTC 2 cut(s) 462, 537
Eco130I CCWWGG 2 cut(s) 316, 438
Eco57I CTGAAG 3 cut(s) 156, 393, 447
EcoNI CCTNNNNNAGG 1 cut(s) 162
EcoT14I CCWWGG 2 cut(s) 316, 438
ErhI CCWWGG 2 cut(s) 316, 438
FaeI CATG 3 cut(s) 197, 408, 427
FaqI GGGAC 1 cut(s) 423
FatI CATG 3 cut(s) 193, 404, 423
FauNDI CATATG 1 cut(s) 44
Fnu4HI GCNGC 2 cut(s) 125, 379
FokI GGATG 1 cut(s) 92
Fsp4HI GCNGC 2 cut(s) 125, 379
GluI GCNGC 2 cut(s) 125, 379
HaeIII GGCC 1 cut(s) 437
Hin1II CATG 3 cut(s) 197, 408, 427
HinfI GANTC 1 cut(s) 322
HphI GGTGA 1 cut(s) 242
Hpy166II GTNNAC 2 cut(s) 59, 233
Hpy188I TCNGA 5 cut(s) 111, 146, 373, 485, 615
Hpy188III TCNNGA 3 cut(s) 92, 326, 397
Hpy8I GTNNAC 2 cut(s) 59, 233
HpyAV CCTTC 3 cut(s) 158, 368, 471
HpyCH4III ACNGT 1 cut(s) 599
HpyCH4V TGCA 2 cut(s) 127, 674
HpyF10VI GCNNNNNNNGC 1 cut(s) 124
HpyF3I CTNAG 3 cut(s) 108, 240, 680
Hsp92II CATG 3 cut(s) 197, 408, 427
Kzo9I GATC 3 cut(s) 88, 189, 196
LmnI GCTCC 1 cut(s) 518
Lsp1109I GCAGC 2 cut(s) 111, 365
LweI GCATC 1 cut(s) 127
MalI GATC 3 cut(s) 90, 191, 198
MboI GATC 3 cut(s) 88, 189, 196
MboII GAAGA 4 cut(s) 20, 209, 449, 554
MlsI TGGCCA 1 cut(s) 437
MluCI AATT 3 cut(s) 363, 556, 654
MluNI TGGCCA 1 cut(s) 437
MnlI CCTC 3 cut(s) 117, 249, 538
Mox20I TGGCCA 1 cut(s) 437
MroXI GAANNNNTTC 1 cut(s) 665
MscI TGGCCA 1 cut(s) 437
MseI TTAA 3 cut(s) 179, 213, 387
Msp20I TGGCCA 1 cut(s) 437
MspA1I CMGCKG 1 cut(s) 467
Mva1269I GAATGC 1 cut(s) 56
MwoI GCNNNNNNNGC 1 cut(s) 124
NdeI CATATG 1 cut(s) 44
NdeII GATC 3 cut(s) 88, 189, 196
NlaIII CATG 3 cut(s) 197, 408, 427
NspI RCATGY 1 cut(s) 427
PciI ACATGT 1 cut(s) 423
PctI GAATGC 1 cut(s) 56
PdmI GAANNNNTTC 1 cut(s) 665
PfeI GAWTC 1 cut(s) 322
PkrI GCNGC 2 cut(s) 126, 380
PscI ACATGT 1 cut(s) 423
PsiI TTATAA 1 cut(s) 348
PstI CTGCAG 1 cut(s) 129
PvuII CAGCTG 1 cut(s) 467
RsaI GTAC 2 cut(s) 428, 665
RsaNI GTAC 2 cut(s) 427, 664
SaqAI TTAA 3 cut(s) 179, 213, 387
SatI GCNGC 2 cut(s) 125, 379
Sau3AI GATC 3 cut(s) 88, 189, 196
ScaI AGTACT 1 cut(s) 665
SetI ASST 5 cut(s) 33, 142, 210, 469, 594
SfaNI GCATC 1 cut(s) 127
SfcI CTRYAG 2 cut(s) 125, 331
Sse9I AATT 3 cut(s) 363, 556, 654
SsiI CCGC 1 cut(s) 381
StyI CCWWGG 2 cut(s) 316, 438
TaaI ACNGT 1 cut(s) 599
TasI AATT 3 cut(s) 363, 556, 654
TatI WGTACW 2 cut(s) 426, 663
TfiI GAWTC 1 cut(s) 322
Tru1I TTAA 3 cut(s) 179, 213, 387
Tru9I TTAA 3 cut(s) 179, 213, 387
TscAI CASTG 2 cut(s) 248, 358
TseI GCWGC 2 cut(s) 124, 378
TspDTI ATGAA 6 cut(s) 84, 290, 466, 483, 551, 555
TspRI CASTG 2 cut(s) 248, 358
XagI CCTNNNNNAGG 1 cut(s) 162
XapI RAATTY 2 cut(s) 363, 556
XceI RCATGY 1 cut(s) 427
XmnI GAANNNNTTC 1 cut(s) 665
ZrmI AGTACT 1 cut(s) 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.