MD17G1179500.v1.1

Inositol oxygenase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001948
Physical Location & Seq
Forward (+)
20763960 .. 20766965
3006 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1179500.v1.1.491

Sequence Viewer

Length: 933 bp
ATGACTATCCTCATTGAGCAGCCTGAGTTTGTGATAGGAGTTGAAGTTGAAATTGAAGCAGGGGAGAGAAAGGTGTTAAATGATGAAAAGGAATTGGTCTTAGATGCTGGATTTGTGGTGCCACATGCCAATTCATTTGGACAAACATTTAGGGATTATGATGCAGAAGGAGAGAGACAACCAAGTGTGGAGAATTTCTACCAGATCAATCACATCAATCAGACTTATGATTTTGTGCAGAGAATGAGAAATGAGTATAGCAAATTGAACAGAGTGGAGATGAGCATTTGGGAATGCTGTGAACTTCTCAACGAGGTTGTCGACGAGAGCGATCCCGACTTGGACGAGCCTCAAATCGAGCACTTGTTGCAAACAGCTGAAGCCATTAGGAAGGACTATCCTAATGAAGATTGGCTGCACTTGACTGGCCTCATCCATGACCTTGGAAAAGTGCTTCTTCTTCCTAGCTTTGGGGAGCTTCCTCAATGGGCAGTTGTCGGTGATACATTCCCAGTTGGCTGTGCTTTTGATCAATCCAATGTTCATCACAAGTACTTTGCGGAAAATCCTGACTACTATAATCCTACTTACAACACTAAGTATGGAGTTTACTCGGAGGGATGTGGACTCGAAAATGTTATGATGTCGTGGGGGCACGATGATTACATGTATTTGGTGGCCACAGAGAACAAATCAACTCTGCCTTCAGCAGCTCTGTTTATCATTAGATACCACTCATTCTATGCCTTACATAAGGCGGGAGCGTATAAGCACTTAATGAACGAAGAGGATGTCGAAAACCTGAAATGGCTCCGTGTATTCAACAAGTACGACCTTTACAGCAAGAGCAAGATACGGGTTGACGTTGAGAAGGTGAAGCCATACTATATTTCTCTCATTGAAAAGTACTTCCCTTCGAAGCTAAGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016701 GO:0050113 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00053 ko00562 map00053 map00562
Pfam Domains
Protein Families
MIOX

Protein Analysis

311

Amino Acids

36.27

Weight (kDa)

4.84

Isoelectric Point (pI)

48.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIOX PF05153 62 - 310 4.2e-126 Myo-inositol oxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 118
AccI GTMKAC 1 cut(s) 321
AciI CCGC 2 cut(s) 560, 758
AclWI GGATC 1 cut(s) 326
AcoI YGGCCR 1 cut(s) 678
AcsI RAATTY 1 cut(s) 193
AcuI CTGAAG 2 cut(s) 399, 690
AfaI GTAC 3 cut(s) 554, 830, 908
AfiI CCNNNNNNNGG 1 cut(s) 470
AflIII ACRYGT 1 cut(s) 666
AgsI TTSAA 6 cut(s) 44, 50, 56, 268, 823, 902
AluBI AGCT 5 cut(s) 377, 468, 478, 713, 922
AluI AGCT 5 cut(s) 377, 468, 478, 713, 922
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 1 cut(s) 169
AlwI GGATC 1 cut(s) 326
AoxI GGCC 2 cut(s) 427, 678
ApeKI GCWGC 3 cut(s) 19, 415, 710
ApoI RAATTY 1 cut(s) 193
ArsI GACNNNNNNTTYG 2 cut(s) 777, 809
AsuHPI GGTGA 2 cut(s) 512, 886
AsuII TTCGAA 1 cut(s) 917
BaeGI GKGCMC 1 cut(s) 657
BalI TGGCCA 1 cut(s) 680
BanI GGYRCC 1 cut(s) 118
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 3 cut(s) 31, 402, 722
BccI CCATC 1 cut(s) 921
BclI TGATCA 1 cut(s) 529
BcoDI GTCTC 1 cut(s) 169
BfaI CTAG 1 cut(s) 465
BisI GCNGC 3 cut(s) 20, 416, 711
BlsI GCNGC 3 cut(s) 21, 417, 712
BmcAI AGTACT 2 cut(s) 554, 908
BmiI GGNNCC 2 cut(s) 120, 812
BmrI ACTGGG 1 cut(s) 506
BmsI GCATC 2 cut(s) 94, 151
BmuI ACTGGG 1 cut(s) 506
Bpu14I TTCGAA 1 cut(s) 917
BsaJI CCNNGG 1 cut(s) 442
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 2 cut(s) 430, 512
BseDI CCNNGG 1 cut(s) 442
BseGI GGATG 3 cut(s) 432, 626, 796
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMII CTCAG 1 cut(s) 15
BseNI ACTGG 2 cut(s) 430, 512
BseSI GKGCMC 1 cut(s) 657
BseXI GCAGC 3 cut(s) 31, 402, 722
BsgI GTGCAG 2 cut(s) 257, 401
BshFI GGCC 2 cut(s) 429, 680
BshNI GGYRCC 1 cut(s) 118
BsiHKAI GWGCWC 1 cut(s) 363
BslI CCNNNNNNNGG 1 cut(s) 470
BsmAI GTCTC 1 cut(s) 169
BsmI GAATGC 1 cut(s) 299
BsnI GGCC 2 cut(s) 429, 680
Bsp119I TTCGAA 1 cut(s) 917
Bsp1286I GDGCHC 2 cut(s) 363, 657
Bsp143I GATC 3 cut(s) 204, 331, 529
BspACI CCGC 2 cut(s) 560, 758
BspANI GGCC 2 cut(s) 429, 680
BspCNI CTCAG 1 cut(s) 16
BspLI GGNNCC 2 cut(s) 120, 812
BspPI GGATC 1 cut(s) 326
BspT104I TTCGAA 1 cut(s) 917
BspT107I GGYRCC 1 cut(s) 118
BsrI ACTGG 2 cut(s) 430, 512
BssECI CCNNGG 1 cut(s) 442
BssMI GATC 3 cut(s) 204, 331, 529
BssT1I CCWWGG 1 cut(s) 442
Bst6I CTCTTC 1 cut(s) 780
BstAPI GCANNNNNTGC 1 cut(s) 367
BstBI TTCGAA 1 cut(s) 917
BstDEI CTNAG 4 cut(s) 24, 100, 597, 923
BstF5I GGATG 3 cut(s) 432, 626, 796
BstKTI GATC 3 cut(s) 207, 334, 532
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 3 cut(s) 204, 331, 529
BstMWI GCNNNNNNNGC 1 cut(s) 367
BstNSI RCATGY 2 cut(s) 128, 670
BstSLI GKGCMC 1 cut(s) 657
BstV1I GCAGC 3 cut(s) 31, 402, 722
BstXI CCANNNNNNTGG 1 cut(s) 443
BsuRI GGCC 2 cut(s) 429, 680
BtsCI GGATG 3 cut(s) 432, 626, 796
Csp6I GTAC 3 cut(s) 553, 829, 907
CviAII CATG 3 cut(s) 125, 437, 667
CviQI GTAC 3 cut(s) 553, 829, 907
DdeI CTNAG 4 cut(s) 24, 100, 597, 923
DpnI GATC 3 cut(s) 206, 333, 531
DpnII GATC 3 cut(s) 204, 331, 529
EaeI YGGCCR 1 cut(s) 678
Eam1104I CTCTTC 1 cut(s) 780
EarI CTCTTC 1 cut(s) 780
Eco130I CCWWGG 1 cut(s) 442
Eco57I CTGAAG 2 cut(s) 399, 690
EcoT14I CCWWGG 1 cut(s) 442
ErhI CCWWGG 1 cut(s) 442
FaeI CATG 3 cut(s) 128, 440, 670
FalI AAGNNNNNCTT 2 cut(s) 441, 473
FatI CATG 3 cut(s) 124, 436, 666
FauI CCCGC 1 cut(s) 751
FbaI TGATCA 1 cut(s) 529
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 3 cut(s) 20, 416, 711
FokI GGATG 3 cut(s) 419, 633, 803
Fsp4HI GCNGC 3 cut(s) 20, 416, 711
FspBI CTAG 1 cut(s) 465
GluI GCNGC 3 cut(s) 20, 416, 711
HaeIII GGCC 2 cut(s) 429, 680
Hin1II CATG 3 cut(s) 128, 440, 670
HincII GTYRAC 2 cut(s) 322, 862
HindII GTYRAC 2 cut(s) 322, 862
HinfI GANTC 1 cut(s) 627
HphI GGTGA 2 cut(s) 512, 886
Hpy166II GTNNAC 5 cut(s) 302, 322, 610, 626, 862
Hpy188I TCNGA 2 cut(s) 222, 616
Hpy188III TCNNGA 2 cut(s) 335, 569
Hpy8I GTNNAC 5 cut(s) 302, 322, 610, 626, 862
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 5 cut(s) 161, 385, 714, 865, 924
HpyCH4IV ACGT 1 cut(s) 864
HpyCH4V TGCA 4 cut(s) 164, 238, 370, 418
HpyF10VI GCNNNNNNNGC 1 cut(s) 367
HpyF3I CTNAG 4 cut(s) 24, 100, 597, 923
HpySE526I ACGT 1 cut(s) 864
Hsp92II CATG 3 cut(s) 128, 440, 670
Ksp22I TGATCA 1 cut(s) 529
Kzo9I GATC 3 cut(s) 204, 331, 529
LmnI GCTCC 3 cut(s) 475, 761, 816
LpnPI CCDG 8 cut(s) 36, 45, 93, 215, 411, 525, 582, 815
Lsp1109I GCAGC 3 cut(s) 31, 402, 722
LweI GCATC 2 cut(s) 94, 151
MaeI CTAG 1 cut(s) 465
MaeII ACGT 1 cut(s) 864
MalI GATC 3 cut(s) 206, 333, 531
MboI GATC 3 cut(s) 204, 331, 529
MboII GAAGA 4 cut(s) 419, 449, 452, 797
MhlI GDGCHC 2 cut(s) 363, 657
MlsI TGGCCA 1 cut(s) 680
MluCI AATT 5 cut(s) 51, 92, 130, 193, 263
MluNI TGGCCA 1 cut(s) 680
MlyI GAGTC 1 cut(s) 621
MnlI CCTC 7 cut(s) 20, 307, 360, 440, 492, 610, 781
Mox20I TGGCCA 1 cut(s) 680
MscI TGGCCA 1 cut(s) 680
MseI TTAA 2 cut(s) 77, 776
Msp20I TGGCCA 1 cut(s) 680
MspA1I CMGCKG 1 cut(s) 377
Mva1269I GAATGC 1 cut(s) 299
MwoI GCNNNNNNNGC 1 cut(s) 367
NdeII GATC 3 cut(s) 204, 331, 529
NlaIII CATG 3 cut(s) 128, 440, 670
NlaIV GGNNCC 2 cut(s) 120, 812
NspI RCATGY 2 cut(s) 128, 670
NspV TTCGAA 1 cut(s) 917
PciI ACATGT 1 cut(s) 666
PcsI WCGNNNNNNNCGW 2 cut(s) 318, 327
PctI GAATGC 1 cut(s) 299
PkrI GCNGC 3 cut(s) 21, 417, 712
PleI GAGTC 1 cut(s) 621
PpsI GAGTC 1 cut(s) 621
PscI ACATGT 1 cut(s) 666
PspN4I GGNNCC 2 cut(s) 120, 812
PvuII CAGCTG 1 cut(s) 377
RsaI GTAC 3 cut(s) 554, 830, 908
RsaNI GTAC 3 cut(s) 553, 829, 907
SalI GTCGAC 1 cut(s) 320
SaqAI TTAA 2 cut(s) 77, 776
SatI GCNGC 3 cut(s) 20, 416, 711
Sau3AI GATC 3 cut(s) 204, 331, 529
ScaI AGTACT 2 cut(s) 554, 908
SchI GAGTC 1 cut(s) 621
SduI GDGCHC 2 cut(s) 363, 657
SfaNI GCATC 2 cut(s) 94, 151
SfuI TTCGAA 1 cut(s) 917
Sse9I AATT 5 cut(s) 51, 92, 130, 193, 263
SsiI CCGC 2 cut(s) 560, 758
SspMI CTAG 1 cut(s) 465
StyI CCWWGG 1 cut(s) 442
TaiI ACGT 1 cut(s) 867
TaqI TCGA 5 cut(s) 321, 357, 630, 795, 917
TasI AATT 5 cut(s) 51, 92, 130, 193, 263
TatI WGTACW 2 cut(s) 552, 906
Tru1I TTAA 2 cut(s) 77, 776
Tru9I TTAA 2 cut(s) 77, 776
TseI GCWGC 3 cut(s) 19, 415, 710
TspDTI ATGAA 5 cut(s) 99, 123, 420, 533, 794
TspGWI ACGGA 1 cut(s) 803
XapI RAATTY 1 cut(s) 193
XceI RCATGY 2 cut(s) 128, 670
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 1 cut(s) 465
ZrmI AGTACT 2 cut(s) 554, 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.