FvH4_6g32720

Inositol oxygenase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001948
Physical Location & Seq
Reverse (-)
25788792 .. 25791977
3186 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g32720.t3

Sequence Viewer

Length: 939 bp
ATGACCATCCTCATTGAGCAGCAGCAGCAGCAGCAACCTGAGTTTGGAGTTGAAGTTGGAGGAGGGAAGGAGGTTATTGTCGATGAGAAAGAATTGGTCTTGGATGGTGGATTTTTGGTGCCACATACCAACTCATTTGGACAAACTTTTAGGGATTATGATGCAGAAGGTGAGAGGCAACCAAGTGTGGAGAAATTCTACAAGATCAATCACATTCACCAGACTTATGACTTCGTCCAGAGAATGAGAAAAGAGTACAGCAAATTGAACAGGGTGGAGATGAGCATATGGGAATGCTGTGAACTTCTCAATGAGGTTGTAGACGAGAGTGATCCGGACTTGGATGAACCTCAGATTGAGCATTTGTTGCAAACAGCTGAAGCCATTAGAAAGGACTATCCCAATGAAGATTGGCTACACTTGACTGGCCTTATCCATGATCTTGGGAAGGTGCTTCTTCTTCCTTGCTTTGGGGAGCTTCCTCAATGGGCTGTTGTAGGTGACACTTTCCCAGTCGGTTGTGCTTTCGACGAATCCAATGTTCACCATAAGTACTTCAAGGAGAACCCCGACTACAACAATCCGGCTTACAACACTAAACATGGGGTTTACTCTGATGAGAGGTGTGGACTCAACAACGTACTGATGTCATGGGGGCATGATGACTACATGTATCTGGTGGCCAAAGAGAACAAGACAACTCTACCTTCAGCAGGTCTTTTCGTCATTAGATACCACTCGTTCTATGCTTTACACACAGCAGGAGCCTACAAGCACTTGATGAATGAAGAGGATGTCGACAACTTGAAGTGGCTTCATGTATTCAACAAGTATGACCTTTACAGCAAGAGCAAAGTTCGGGTTGATGTTGAGAAGGTGAAGCCGTACTATCTCTCACTCATAGAAAAGTACTTCCCAACTGCCAAGCTAAGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016701 GO:0050113 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00053 ko00562 map00053 map00562
Pfam Domains
Protein Families
MIOX

Protein Analysis

313

Amino Acids

36.45

Weight (kDa)

5.18

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIOX PF05153 1 - 231 5.2e-116 Myo-inositol oxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 704
AccB1I GGYRCC 1 cut(s) 118
AccI GTMKAC 2 cut(s) 321, 798
AccIII TCCGGA 1 cut(s) 334
AclWI GGATC 1 cut(s) 326
AcoI YGGCCR 1 cut(s) 681
AcsI RAATTY 1 cut(s) 194
AcuI CTGAAG 2 cut(s) 399, 693
AfaI GTAC 5 cut(s) 257, 554, 642, 887, 911
AfiI CCNNNNNNNGG 3 cut(s) 44, 470, 713
AflIII ACRYGT 1 cut(s) 669
AgsI TTSAA 5 cut(s) 53, 268, 559, 808, 826
AluBI AGCT 3 cut(s) 377, 478, 928
AluI AGCT 3 cut(s) 377, 478, 928
AlwI GGATC 1 cut(s) 326
Aor13HI TCCGGA 1 cut(s) 334
AoxI GGCC 2 cut(s) 427, 681
ApeKI GCWGC 5 cut(s) 19, 22, 25, 28, 31
ApoI RAATTY 1 cut(s) 194
AsuHPI GGTGA 5 cut(s) 182, 209, 512, 536, 889
BalI TGGCCA 1 cut(s) 683
BanI GGYRCC 1 cut(s) 118
BarI GAAGNNNNNNTAC 2 cut(s) 399, 431
BbvI GCAGC 5 cut(s) 31, 34, 37, 40, 43
BccI CCATC 3 cut(s) 14, 98, 927
BceAI ACGGC 1 cut(s) 868
BfuAI ACCTGC 1 cut(s) 704
BisI GCNGC 5 cut(s) 20, 23, 26, 29, 32
BlsI GCNGC 5 cut(s) 21, 24, 27, 30, 33
BmcAI AGTACT 2 cut(s) 554, 911
BmiI GGNNCC 2 cut(s) 120, 766
BmrI ACTGGG 1 cut(s) 506
BmsI GCATC 1 cut(s) 151
BmuI ACTGGG 1 cut(s) 506
BsaWI WCCGGW 1 cut(s) 334
BsaXI ACNNNNNCTCC 2 cut(s) 39, 69
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 470, 713
Bse1I ACTGG 2 cut(s) 430, 512
BseAI TCCGGA 1 cut(s) 334
BseGI GGATG 4 cut(s) 6, 109, 349, 799
BseLI CCNNNNNNNGG 3 cut(s) 44, 470, 713
BseMII CTCAG 2 cut(s) 30, 365
BseNI ACTGG 2 cut(s) 430, 512
BseRI GAGGAG 1 cut(s) 75
BseXI GCAGC 5 cut(s) 31, 34, 37, 40, 43
BshFI GGCC 2 cut(s) 429, 683
BshNI GGYRCC 1 cut(s) 118
BsiSI CCGG 2 cut(s) 335, 584
BslI CCNNNNNNNGG 3 cut(s) 44, 470, 713
BsmI GAATGC 1 cut(s) 299
BsnI GGCC 2 cut(s) 429, 683
Bsp13I TCCGGA 1 cut(s) 334
Bsp143I GATC 3 cut(s) 204, 331, 439
BspANI GGCC 2 cut(s) 429, 683
BspCNI CTCAG 2 cut(s) 31, 364
BspEI TCCGGA 1 cut(s) 334
BspLI GGNNCC 2 cut(s) 120, 766
BspMI ACCTGC 1 cut(s) 704
BspPI GGATC 1 cut(s) 326
BspT107I GGYRCC 1 cut(s) 118
BsrI ACTGG 2 cut(s) 430, 512
BssMI GATC 3 cut(s) 204, 331, 439
Bst6I CTCTTC 1 cut(s) 783
BstAPI GCANNNNNTGC 1 cut(s) 367
BstDEI CTNAG 3 cut(s) 39, 351, 929
BstENI CCTNNNNNAGG 1 cut(s) 711
BstF5I GGATG 4 cut(s) 6, 109, 349, 799
BstKTI GATC 3 cut(s) 207, 334, 442
BstMBI GATC 3 cut(s) 204, 331, 439
BstMWI GCNNNNNNNGC 4 cut(s) 25, 28, 31, 367
BstNSI RCATGY 1 cut(s) 673
BstV1I GCAGC 5 cut(s) 31, 34, 37, 40, 43
BstXI CCANNNNNNTGG 1 cut(s) 443
BsuRI GGCC 2 cut(s) 429, 683
BtsCI GGATG 4 cut(s) 6, 109, 349, 799
BveI ACCTGC 1 cut(s) 704
Csp6I GTAC 5 cut(s) 256, 553, 641, 886, 910
CviAII CATG 6 cut(s) 437, 602, 651, 659, 670, 818
CviQI GTAC 5 cut(s) 256, 553, 641, 886, 910
DdeI CTNAG 3 cut(s) 39, 351, 929
DpnI GATC 3 cut(s) 206, 333, 441
DpnII GATC 3 cut(s) 204, 331, 439
EaeI YGGCCR 1 cut(s) 681
Eam1104I CTCTTC 1 cut(s) 783
EarI CTCTTC 1 cut(s) 783
Eco57I CTGAAG 2 cut(s) 399, 693
EcoNI CCTNNNNNAGG 1 cut(s) 711
FaeI CATG 6 cut(s) 440, 605, 654, 662, 673, 821
FatI CATG 6 cut(s) 436, 601, 650, 658, 669, 817
FauNDI CATATG 1 cut(s) 287
FblI GTMKAC 2 cut(s) 321, 798
Fnu4HI GCNGC 5 cut(s) 20, 23, 26, 29, 32
FokI GGATG 3 cut(s) 116, 356, 806
Fsp4HI GCNGC 5 cut(s) 20, 23, 26, 29, 32
GluI GCNGC 5 cut(s) 20, 23, 26, 29, 32
HaeIII GGCC 2 cut(s) 429, 683
HapII CCGG 2 cut(s) 335, 584
Hin1II CATG 6 cut(s) 440, 605, 654, 662, 673, 821
HincII GTYRAC 1 cut(s) 799
HindII GTYRAC 1 cut(s) 799
HinfI GANTC 2 cut(s) 533, 630
HpaII CCGG 2 cut(s) 335, 584
HphI GGTGA 5 cut(s) 182, 209, 512, 536, 889
Hpy166II GTNNAC 6 cut(s) 302, 322, 544, 610, 629, 799
Hpy188I TCNGA 2 cut(s) 354, 616
Hpy188III TCNNGA 2 cut(s) 238, 335
Hpy8I GTNNAC 6 cut(s) 302, 322, 544, 610, 629, 799
Hpy99I CGWCG 1 cut(s) 533
HpyAV CCTTC 5 cut(s) 61, 161, 442, 717, 868
HpyCH4IV ACGT 1 cut(s) 639
HpyCH4V TGCA 2 cut(s) 164, 370
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 28, 31, 367
HpyF3I CTNAG 3 cut(s) 39, 351, 929
HpySE526I ACGT 1 cut(s) 639
Hsp92II CATG 6 cut(s) 440, 605, 654, 662, 673, 821
Kpn2I TCCGGA 1 cut(s) 334
Kzo9I GATC 3 cut(s) 204, 331, 439
LmnI GCTCC 2 cut(s) 475, 764
Lsp1109I GCAGC 5 cut(s) 31, 34, 37, 40, 43
LweI GCATC 1 cut(s) 151
MaeII ACGT 1 cut(s) 639
MaeIII GTNAC 1 cut(s) 500
MalI GATC 3 cut(s) 206, 333, 441
MboI GATC 3 cut(s) 204, 331, 439
MboII GAAGA 4 cut(s) 419, 449, 452, 800
MlsI TGGCCA 1 cut(s) 683
MluCI AATT 3 cut(s) 92, 194, 263
MluNI TGGCCA 1 cut(s) 683
MlyI GAGTC 1 cut(s) 624
MmeI TCCRAC 1 cut(s) 37
Mox20I TGGCCA 1 cut(s) 683
MroI TCCGGA 1 cut(s) 334
MscI TGGCCA 1 cut(s) 683
Msp20I TGGCCA 1 cut(s) 683
MspA1I CMGCKG 1 cut(s) 377
MspI CCGG 2 cut(s) 335, 584
Mva1269I GAATGC 1 cut(s) 299
MwoI GCNNNNNNNGC 4 cut(s) 25, 28, 31, 367
NdeI CATATG 1 cut(s) 287
NdeII GATC 3 cut(s) 204, 331, 439
NlaIII CATG 6 cut(s) 440, 605, 654, 662, 673, 821
NlaIV GGNNCC 2 cut(s) 120, 766
NmuCI GTSAC 1 cut(s) 500
NspI RCATGY 1 cut(s) 673
PciI ACATGT 1 cut(s) 669
PctI GAATGC 1 cut(s) 299
PfeI GAWTC 1 cut(s) 533
PflFI GACNNNGTC 1 cut(s) 233
PkrI GCNGC 5 cut(s) 21, 24, 27, 30, 33
PleI GAGTC 1 cut(s) 624
PpsI GAGTC 1 cut(s) 624
PscI ACATGT 1 cut(s) 669
PspN4I GGNNCC 2 cut(s) 120, 766
PsrI GAACNNNNNNTAC 2 cut(s) 557, 589
PsyI GACNNNGTC 1 cut(s) 233
PvuII CAGCTG 1 cut(s) 377
RsaI GTAC 5 cut(s) 257, 554, 642, 887, 911
RsaNI GTAC 5 cut(s) 256, 553, 641, 886, 910
SalI GTCGAC 1 cut(s) 797
SatI GCNGC 5 cut(s) 20, 23, 26, 29, 32
Sau3AI GATC 3 cut(s) 204, 331, 439
ScaI AGTACT 2 cut(s) 554, 911
SchI GAGTC 1 cut(s) 624
SfaNI GCATC 1 cut(s) 151
Sse9I AATT 3 cut(s) 92, 194, 263
TaiI ACGT 1 cut(s) 642
TaqI TCGA 3 cut(s) 81, 528, 798
TasI AATT 3 cut(s) 92, 194, 263
TatI WGTACW 3 cut(s) 255, 552, 909
TfiI GAWTC 1 cut(s) 533
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 5 cut(s) 19, 22, 25, 28, 31
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 5 cut(s) 360, 420, 797, 801, 806
Tth111I GACNNNGTC 1 cut(s) 233
XagI CCTNNNNNAGG 1 cut(s) 711
XapI RAATTY 1 cut(s) 194
XceI RCATGY 1 cut(s) 673
XmiI GTMKAC 2 cut(s) 321, 798
ZrmI AGTACT 2 cut(s) 554, 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.