Rw4G036700

Myo-inositol oxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001948
Physical Location & Seq
Forward (+)
63529377 .. 63532724
3348 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G036700.1

Sequence Viewer

Length: 918 bp
ATGACGATCCTCATCGAGCAGCCGGAATTTGTTGAGGTGGAGGAGGAGAAGGTTTGTTCCGAAGAGAATGAGAATGAACTGGTGTTGGATGGTGGATTTGTGGTGCCGACGACCAACGCATTTGGCCACACTTTTAGGGATTATGATGTTAATAGTGAGCGGAGAGATGGGGTTGGGGAATTCTACCGAATCCAGCACATCAACCAAAGTGTTGATTTTGTGAAGCGGATGAGAGAAGAATATGGAAAATTGGACAAGGTGGAAATGAGCATATGGGAATGCTGTGAACTTTTGAATGAAGTTGTGGATGAGAGTGATCCTGATTTGGATGAGCCTCAGATTGAGCATCTGCTGCAGACTGCTGAAGCTATCAGAAAAGACTACCCTAAAGAAGGCTGGTTACATTTGACTGCCCTAATCCATGATCTTGGAAAGGTGCTTCATCTTCCTGCTTTTGGTGAGCTTCCTCAGTGGGCTGTCGTAGGAGACACATTTCCTGTTGGGTGTGCCTTTGATGAGTCAATTGTTCACCACAAGCATTTCCAGGAAAATCCTGACTACAGCAATCCTGCTTATAACACCAAATATGGAATTTACTCTGAAGGCTGTGGACTTAACAATGTCCTGATGTCATGGGGACACGATGACTACATGTTCTTGGTGGCCAAAGGGAATAACTCTACTCTTCCTTCAGCTGGTCTTTTCATCATCAGATACCATTCATTTTATGCTTTACACAGGGCAGGGGCATATAAGCACTTGATGAATGAAGATGACATTGAGAATTTGAAGTGGCTCAAAGTGTTCAACAAGTATGACCTTTACAGTAAGAGCAAAGTTCGCATTGATGTTGAGAAAGTCAAGCCCTACTATCTCTCTCTAATTGAGAAGTACTTTCCTGCAAAGTTGAGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko00562 map00053 map00562
Pfam Domains
Protein Families
MIOX

Protein Analysis

305

Amino Acids

35.48

Weight (kDa)

4.99

Isoelectric Point (pI)

47.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIOX PF05153 56 - 305 5.2e-125 Myo-inositol oxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 576
AccB1I GGYRCC 1 cut(s) 103
AccBSI CCGCTC 1 cut(s) 160
AciI CCGC 2 cut(s) 160, 226
AclWI GGATC 1 cut(s) 311
AcoI YGGCCR 2 cut(s) 124, 663
AcsI RAATTY 4 cut(s) 26, 179, 591, 784
AcuI CTGAAG 3 cut(s) 384, 621, 675
AfaI GTAC 1 cut(s) 893
AfiI CCNNNNNNNGG 3 cut(s) 392, 455, 695
AflIII ACRYGT 1 cut(s) 651
AgsI TTSAA 3 cut(s) 295, 790, 808
AjnI CCWGG 1 cut(s) 543
AluBI AGCT 3 cut(s) 368, 463, 695
AluI AGCT 3 cut(s) 368, 463, 695
Alw26I GTCTC 1 cut(s) 480
AlwI GGATC 1 cut(s) 311
AoxI GGCC 2 cut(s) 124, 663
ApeKI GCWGC 2 cut(s) 19, 352
ApoI RAATTY 4 cut(s) 26, 179, 591, 784
AsuHPI GGTGA 2 cut(s) 470, 521
BalI TGGCCA 2 cut(s) 126, 665
BanI GGYRCC 1 cut(s) 103
BarI GAAGNNNNNNTAC 2 cut(s) 384, 416
BbvI GCAGC 2 cut(s) 31, 339
BccI CCATC 3 cut(s) 83, 161, 906
BciT130I CCWGG 1 cut(s) 545
BcoDI GTCTC 1 cut(s) 480
BfmI CTRYAG 2 cut(s) 353, 559
BisI GCNGC 2 cut(s) 20, 353
BlsI GCNGC 2 cut(s) 21, 354
BmcAI AGTACT 1 cut(s) 893
Bme1390I CCNGG 1 cut(s) 545
BmiI GGNNCC 1 cut(s) 105
BmrFI CCNGG 1 cut(s) 545
BmsI GCATC 1 cut(s) 355
BsaBI GATNNNNATC 1 cut(s) 11
Bsc4I CCNNNNNNNGG 3 cut(s) 392, 455, 695
Bse1I ACTGG 1 cut(s) 84
Bse8I GATNNNNATC 1 cut(s) 11
BseBI CCWGG 1 cut(s) 545
BseGI GGATG 4 cut(s) 94, 234, 313, 334
BseJI GATNNNNATC 1 cut(s) 11
BseLI CCNNNNNNNGG 3 cut(s) 392, 455, 695
BseMII CTCAG 2 cut(s) 350, 482
BseNI ACTGG 1 cut(s) 84
BseRI GAGGAG 2 cut(s) 56, 59
BseXI GCAGC 2 cut(s) 31, 339
BshFI GGCC 2 cut(s) 126, 665
BshNI GGYRCC 1 cut(s) 103
BsiSI CCGG 1 cut(s) 23
BslFI GGGAC 1 cut(s) 651
BslI CCNNNNNNNGG 3 cut(s) 392, 455, 695
BsmAI GTCTC 1 cut(s) 480
BsmFI GGGAC 1 cut(s) 651
BsmI GAATGC 1 cut(s) 284
BsnI GGCC 2 cut(s) 126, 665
Bsp143I GATC 3 cut(s) 6, 316, 424
BspACI CCGC 2 cut(s) 160, 226
BspANI GGCC 2 cut(s) 126, 665
BspCNI CTCAG 2 cut(s) 349, 481
BspLI GGNNCC 1 cut(s) 105
BspMAI CTGCAG 1 cut(s) 357
BspPI GGATC 1 cut(s) 311
BspT107I GGYRCC 1 cut(s) 103
BsrBI CCGCTC 1 cut(s) 160
BsrI ACTGG 1 cut(s) 84
BssMI GATC 3 cut(s) 6, 316, 424
Bst2UI CCWGG 1 cut(s) 545
Bst4CI ACNGT 1 cut(s) 827
Bst6I CTCTTC 2 cut(s) 57, 690
BstAPI GCANNNNNTGC 1 cut(s) 352
BstDEI CTNAG 2 cut(s) 336, 468
BstENI CCTNNNNNAGG 1 cut(s) 390
BstF5I GGATG 4 cut(s) 94, 234, 313, 334
BstKTI GATC 3 cut(s) 9, 319, 427
BstMAI GTCTC 1 cut(s) 480
BstMBI GATC 3 cut(s) 6, 316, 424
BstMWI GCNNNNNNNGC 2 cut(s) 352, 840
BstNI CCWGG 1 cut(s) 545
BstNSI RCATGY 1 cut(s) 655
BstSCI CCNGG 1 cut(s) 543
BstSFI CTRYAG 2 cut(s) 353, 559
BstV1I GCAGC 2 cut(s) 31, 339
BstXI CCANNNNNNTGG 1 cut(s) 428
BsuRI GGCC 2 cut(s) 126, 665
BtsCI GGATG 4 cut(s) 94, 234, 313, 334
BtsIMutI CAGTG 1 cut(s) 476
Csp6I GTAC 1 cut(s) 892
CviAII CATG 3 cut(s) 422, 633, 652
CviQI GTAC 1 cut(s) 892
DdeI CTNAG 2 cut(s) 336, 468
DpnI GATC 3 cut(s) 8, 318, 426
DpnII GATC 3 cut(s) 6, 316, 424
EaeI YGGCCR 2 cut(s) 124, 663
Eam1104I CTCTTC 2 cut(s) 57, 690
EarI CTCTTC 2 cut(s) 57, 690
Eco57I CTGAAG 3 cut(s) 384, 621, 675
EcoNI CCTNNNNNAGG 1 cut(s) 390
EcoRI GAATTC 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 543
FaeI CATG 3 cut(s) 425, 636, 655
FaqI GGGAC 1 cut(s) 651
FatI CATG 3 cut(s) 421, 632, 651
FauNDI CATATG 1 cut(s) 272
Fnu4HI GCNGC 2 cut(s) 20, 353
FokI GGATG 4 cut(s) 101, 241, 320, 341
Fsp4HI GCNGC 2 cut(s) 20, 353
GluI GCNGC 2 cut(s) 20, 353
HaeIII GGCC 2 cut(s) 126, 665
HapII CCGG 1 cut(s) 23
Hin1II CATG 3 cut(s) 425, 636, 655
HinfI GANTC 2 cut(s) 189, 518
HpaII CCGG 1 cut(s) 23
HphI GGTGA 2 cut(s) 470, 521
Hpy166II GTNNAC 3 cut(s) 287, 529, 611
Hpy188I TCNGA 5 cut(s) 61, 339, 374, 601, 713
Hpy188III TCNNGA 3 cut(s) 320, 554, 625
Hpy8I GTNNAC 3 cut(s) 287, 529, 611
Hpy99I CGWCG 1 cut(s) 112
HpyAV CCTTC 4 cut(s) 43, 386, 596, 699
HpyCH4III ACNGT 1 cut(s) 827
HpyCH4V TGCA 2 cut(s) 355, 902
HpyF10VI GCNNNNNNNGC 2 cut(s) 352, 840
HpyF3I CTNAG 2 cut(s) 336, 468
Hsp92II CATG 3 cut(s) 425, 636, 655
Kzo9I GATC 3 cut(s) 6, 316, 424
Lsp1109I GCAGC 2 cut(s) 31, 339
LweI GCATC 1 cut(s) 355
MaeIII GTNAC 1 cut(s) 399
MalI GATC 3 cut(s) 8, 318, 426
MbiI CCGCTC 1 cut(s) 160
MboI GATC 3 cut(s) 6, 316, 424
MboII GAAGA 5 cut(s) 74, 248, 437, 677, 782
MfeI CAATTG 1 cut(s) 522
MlsI TGGCCA 2 cut(s) 126, 665
MluCI AATT 7 cut(s) 26, 179, 248, 522, 591, 784, 882
MluNI TGGCCA 2 cut(s) 126, 665
MlyI GAGTC 1 cut(s) 527
MmeI TCCRAC 1 cut(s) 66
MnlI CCTC 6 cut(s) 20, 28, 34, 37, 345, 477
Mox20I TGGCCA 2 cut(s) 126, 665
MscI TGGCCA 2 cut(s) 126, 665
MseI TTAA 2 cut(s) 150, 615
Msp20I TGGCCA 2 cut(s) 126, 665
MspA1I CMGCKG 1 cut(s) 695
MspI CCGG 1 cut(s) 23
MspR9I CCNGG 1 cut(s) 545
MunI CAATTG 1 cut(s) 522
Mva1269I GAATGC 1 cut(s) 284
MvaI CCWGG 1 cut(s) 545
MwoI GCNNNNNNNGC 2 cut(s) 352, 840
NdeI CATATG 1 cut(s) 272
NdeII GATC 3 cut(s) 6, 316, 424
NlaIII CATG 3 cut(s) 425, 636, 655
NlaIV GGNNCC 1 cut(s) 105
NspI RCATGY 1 cut(s) 655
PciI ACATGT 1 cut(s) 651
PctI GAATGC 1 cut(s) 284
PfeI GAWTC 1 cut(s) 189
PfoI TCCNGGA 1 cut(s) 543
PkrI GCNGC 2 cut(s) 21, 354
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
PscI ACATGT 1 cut(s) 651
PsiI TTATAA 1 cut(s) 576
Psp6I CCWGG 1 cut(s) 543
PspGI CCWGG 1 cut(s) 543
PspN4I GGNNCC 1 cut(s) 105
PstI CTGCAG 1 cut(s) 357
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 1 cut(s) 893
RsaNI GTAC 1 cut(s) 892
SaqAI TTAA 2 cut(s) 150, 615
SatI GCNGC 2 cut(s) 20, 353
Sau3AI GATC 3 cut(s) 6, 316, 424
ScaI AGTACT 1 cut(s) 893
SchI GAGTC 1 cut(s) 527
ScrFI CCNGG 1 cut(s) 545
SetI ASST 8 cut(s) 39, 54, 261, 370, 438, 465, 697, 822
SfaNI GCATC 1 cut(s) 355
SfcI CTRYAG 2 cut(s) 353, 559
Sse9I AATT 7 cut(s) 26, 179, 248, 522, 591, 784, 882
SsiI CCGC 2 cut(s) 160, 226
StyD4I CCNGG 1 cut(s) 543
TaaI ACNGT 1 cut(s) 827
TaqI TCGA 1 cut(s) 15
TasI AATT 7 cut(s) 26, 179, 248, 522, 591, 784, 882
TatI WGTACW 1 cut(s) 891
TfiI GAWTC 1 cut(s) 189
Tru1I TTAA 2 cut(s) 150, 615
Tru9I TTAA 2 cut(s) 150, 615
TscAI CASTG 1 cut(s) 476
TseI GCWGC 2 cut(s) 19, 352
TspDTI ATGAA 7 cut(s) 90, 312, 431, 694, 711, 779, 783
TspRI CASTG 1 cut(s) 476
XagI CCTNNNNNAGG 1 cut(s) 390
XapI RAATTY 4 cut(s) 26, 179, 591, 784
XceI RCATGY 1 cut(s) 655
ZrmI AGTACT 1 cut(s) 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.