Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0001948

Physical Location & Seq

Genomic Coordinates Forward (+)

47005127 .. 47010388

Gene Structure 5262 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0368400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1365 bp

ATGGCTTCCATATCGGCCGACCTAGCCGCCACCCTTCTCTCCCTAACTTCCCGTCACTCCACCCGTGTCGGAGCCCCGGCCCTATTCCCTCTCACATGGAGACCCCGCACTTCCCCACTGTCTCATACCCTCTGCTACTCCACTTCAACTTCCTCGTTTCGCCAGAGACTCGTCTCCAGCCCTAAGCGGGGCTCGAGGAGGTTCACTGTGGCCGCCACAACTGCCACGCCGCCGCAGAGTGACAGCTCCGACGTTTTGACCCAGATTCCTCCGGATAATCGTATCCCGGCGACGATTATTACCGGCTTCTTGGGCTCCGGAAAGACTACATTGCTTAATCATATATTGACTGCCGACCACGGGAAGCGAATTGCAGTGATTGAAAATGAGTATGGTGAAGTAGACATTGATGGTTCTTTGGTGGCTGCGAAATCCGCTGGGGCCGAAGATATTGTTATGCTGAATAATGGGTGCCTTTGCTGCACTGTGAGGGGTGATCTTGTGCGGATGATTTCAGACTTGGTTAATAAGAAGAAAGGGAGGTTTGATCATATTGTGATAGAGACTACAGGATTGGCAAATCCAGCACCGATAATTCAAACATTTTATGCGGAAGATCAGATTTTCAATGATGTCAAGTTGGACGGTGTTGTCACGTTAGTTGATGCTAAACATGCTCGACTTCATCTGGATGAAGTCAAGGCAGAAGGAGTGGTTAATGAGGCAGTAGAGCAGATTGCTTATGCTGATCGTATCATTGTGAATAAGACTGATCTTGTTGGCGAGTCAGATATTGCTTCTTTGGTGCAGAGAATACGGAACATTAATGGCATGGCTCAACTGAAGCGTACAGAGTTTGGAAAAGTTAACCTGGATTATGTGCTCGGCATCGGAGGATTTGATTTGGAGAGGATAGAGAGTGCTATTGATGCTGAAGCTGAGAAGGAAGATCATGACCATCATCACGACCACCATGACCATCATGATCATCATCACCATCATGATGAAGAACATGATCACAAACATGAACATCATGATGGTCACCACTCGCATGATCATGTTCATGATCCTGGTGTTTCTTCTGTCAGTATTGTTTGCGAAGGGAACCTCGATCTGGAGAAGGCCAACATGTGGCTTGGCACACTGTTGTTGGACCGGAGTGAGGACATATATCGTATGAAAGGTCTTCTATCTGTTCAGGGTATGGATGAGAGATTTGTCTTCCAGGGAGTTCATGATATCTTCCAAGGTTCTCCCGATCGGTTGTGGGGCCCCAATGAGCCAAGAACAAATAAGATTGTTTTCATAGGGAAGAACTTGGATGCACAAGAGTTGGAGAAGGGTTTTAAAGGCTGTTTACTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016701 GO:0050113 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00053 ko00562 map00053 map00562

Pfam Domains

Protein Families

MIOX

Protein Analysis

454

Amino Acids

50.24

Weight (kDa)

5.82

Isoelectric Point (pI)

34.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
cobW	PF02492	96 - 280	2.1e-59	CobW/HypB/UreG, nucleotide-binding domain
CobW_C	PF07683	359 - 453	9.6e-23	Cobalamin synthesis protein cobW C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001948)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14520 AT1G14520 AT1G14520 AT1G14520
fragaria_vesca	FvH4_4g36370 FvH4_4g36370 FvH4_6g32720 FvH4_6g32720 FvH4_6g32720 FvH4_6g32720
malus_domestica	MD16G1007900.v1.1 MD17G1179500.v1.1 MD17G1179700.v1.1
prunus_persica	Prupe.1G343000_v2.0.a1 Prupe.1G343000_v2.0.a1 Prupe.1G343000_v2.0.a1 Prupe.3G055000_v2.0.a1 Prupe.3G055000_v2.0.a1 Prupe.3G055000_v2.0.a1
pyrus_communis	pycom16g00770 pycom17g18890
rosa_chinensis	RchiOBHm_Chr2g0141741 RchiOBHm_Chr4g0446111
rosa_laevigata	RLG00000005691 RLG00000019932
rosa_multiflora	Rmu_co8191254.1_g000001 Rmu_sc0002928.1_g000009 Rmu_sc0030751.1_g000001 Rmu_sc0035578.1_g000001
rosa_roxburghii	Rroxscaffold_2G00103910 Rroxscaffold_5G00386510
rosa_rugosa	Rorug02G0368400 Rorug02G0368500
rosa_samantha	Rh2AG420100 Rh2BG429500 Rh2BG431000 Rh2CG407300 Rh2DG439400 Rh2DG440700 Rh4AG429500 Rh4BG439000 Rh4CG456300 Rh4DG437400
rosa_wichuraiana	Rw2G034450 Rw4G036700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	650
AccB1I	GGYRCC	1 cut(s)	471
AccB7I	CCANNNNNTGG	1 cut(s)	1131
AccI	GTMKAC	1 cut(s)	402
AccIII	TCCGGA	2 cut(s)	271, 317
AciI	CCGC	9 cut(s)	27, 106, 187, 213, 230, 233, 435, 505, 611
AclWI	GGATC	1 cut(s)	1061
AcoI	YGGCCR	2 cut(s)	15, 210
AcuI	CTGAAG	2 cut(s)	863, 954
AfaI	GTAC	1 cut(s)	850
AfiI	CCNNNNNNNGG	6 cut(s)	187, 188, 360, 1114, 1131, 1162
AflIII	ACRYGT	1 cut(s)	1128
AgsI	TTSAA	4 cut(s)	147, 383, 599, 628
AjnI	CCWGG	3 cut(s)	870, 1069, 1224
AluBI	AGCT	2 cut(s)	246, 938
AluI	AGCT	2 cut(s)	246, 938
Alw21I	GWGCWC	1 cut(s)	885
Alw26I	GTCTC	5 cut(s)	94, 126, 160, 178, 557
AlwI	GGATC	1 cut(s)	1061
Ama87I	CYCGRG	1 cut(s)	193
Aor13HI	TCCGGA	2 cut(s)	271, 317
AoxI	GGCC	6 cut(s)	15, 78, 210, 441, 1122, 1270
ApaI	GGGCCC	1 cut(s)	1274
ApeKI	GCWGC	2 cut(s)	425, 480
AseI	ATTAAT	1 cut(s)	825
AspS9I	GGNCC	5 cut(s)	79, 441, 1153, 1270, 1271
AsuC2I	CCSGG	2 cut(s)	77, 287
AsuHPI	GGTGA	4 cut(s)	407, 506, 986, 1034
AvaI	CYCGRG	1 cut(s)	193
AvaII	GGWCC	1 cut(s)	1153
BaeGI	GKGCMC	1 cut(s)	1274
BanI	GGYRCC	1 cut(s)	471
BanII	GRGCYC	4 cut(s)	76, 194, 317, 1274
BbsI	GAAGAC	2 cut(s)	1178, 1213
Bbv12I	GWGCWC	1 cut(s)	885
BbvI	GCAGC	2 cut(s)	412, 467
BccI	CCATC	5 cut(s)	404, 966, 987, 1005, 1031
BciT130I	CCWGG	3 cut(s)	872, 1071, 1226
BciVI	GTATCC	1 cut(s)	293
BclI	TGATCA	4 cut(s)	547, 985, 1015, 1054
BcnI	CCSGG	2 cut(s)	77, 287
BcoDI	GTCTC	5 cut(s)	94, 126, 160, 178, 557
BfaI	CTAG	1 cut(s)	23
BfmI	CTRYAG	1 cut(s)	567
BfuI	GTATCC	1 cut(s)	293
BisI	GCNGC	6 cut(s)	27, 213, 230, 233, 426, 481
BlsI	GCNGC	6 cut(s)	28, 214, 231, 234, 427, 482
Bme1390I	CCNGG	5 cut(s)	77, 287, 872, 1071, 1226
Bme18I	GGWCC	1 cut(s)	1153
BmeT110I	CYCGRG	1 cut(s)	193
BmgT120I	GGNCC	5 cut(s)	79, 441, 1153, 1270, 1271
BmiI	GGNNCC	8 cut(s)	73, 316, 442, 473, 1106, 1271, 1272, 1273
BmrFI	CCNGG	5 cut(s)	77, 287, 872, 1071, 1226
BmsI	GCATC	4 cut(s)	655, 897, 919, 1312
BpiI	GAAGAC	2 cut(s)	1178, 1213
BpmI	CTGGAG	2 cut(s)	160, 1136
Bpu10I	CCTNAGC	1 cut(s)	183
BpuMI	CCSGG	2 cut(s)	77, 287
BsaBI	GATNNNNATC	1 cut(s)	990
BsaI	GGTCTC	1 cut(s)	94
BsaJI	CCNNGG	4 cut(s)	75, 358, 1225, 1246
BsaWI	WCCGGW	3 cut(s)	271, 317, 1155
Bsc4I	CCNNNNNNNGG	6 cut(s)	187, 188, 360, 1114, 1131, 1162
Bse118I	RCCGGY	1 cut(s)	302
Bse3DI	GCAATG	1 cut(s)	329
Bse8I	GATNNNNATC	1 cut(s)	990
BseAI	TCCGGA	2 cut(s)	271, 317
BseBI	CCWGG	3 cut(s)	872, 1071, 1226
BseDI	CCNNGG	4 cut(s)	75, 358, 1225, 1246
BseGI	GGATG	4 cut(s)	513, 697, 1213, 1327
BseJI	GATNNNNATC	1 cut(s)	990
BseLI	CCNNNNNNNGG	6 cut(s)	187, 188, 360, 1114, 1131, 1162
BseMI	GCAATG	1 cut(s)	329
BseMII	CTCAG	1 cut(s)	930
BseRI	GAGGAG	1 cut(s)	211
BseSI	GKGCMC	1 cut(s)	1274
BseX3I	CGGCCG	1 cut(s)	15
BseXI	GCAGC	2 cut(s)	412, 467
BseYI	CCCAGC	1 cut(s)	437
BsgI	GTGCAG	2 cut(s)	466, 827
Bsh1285I	CGRYCG	2 cut(s)	18, 1261
BshFI	GGCC	6 cut(s)	17, 80, 212, 443, 1124, 1272
BshNI	GGYRCC	1 cut(s)	471
BsiEI	CGRYCG	2 cut(s)	18, 1261
BsiHKAI	GWGCWC	1 cut(s)	885
BsiHKCI	CYCGRG	1 cut(s)	193
BsiSI	CCGG	6 cut(s)	77, 272, 287, 303, 318, 1156
BslI	CCNNNNNNNGG	6 cut(s)	187, 188, 360, 1114, 1131, 1162
BsmAI	GTCTC	5 cut(s)	94, 126, 160, 178, 557
BsmBI	CGTCTC	1 cut(s)	178
BsnI	GGCC	6 cut(s)	17, 80, 212, 443, 1124, 1272
Bso31I	GGTCTC	1 cut(s)	94
BsoBI	CYCGRG	1 cut(s)	193
Bsp120I	GGGCCC	1 cut(s)	1270
Bsp1286I	GDGCHC	5 cut(s)	76, 194, 317, 885, 1274
Bsp13I	TCCGGA	2 cut(s)	271, 317
BspACI	CCGC	9 cut(s)	27, 106, 187, 213, 230, 233, 435, 505, 611
BspANI	GGCC	6 cut(s)	17, 80, 212, 443, 1124, 1272
BspCNI	CTCAG	1 cut(s)	931
BspEI	TCCGGA	2 cut(s)	271, 317
BspHI	TCATGA	6 cut(s)	952, 982, 1000, 1033, 1063, 1234
BspLI	GGNNCC	8 cut(s)	73, 316, 442, 473, 1106, 1271, 1272, 1273
BspPI	GGATC	1 cut(s)	1061
BspT107I	GGYRCC	1 cut(s)	471
BspTNI	GGTCTC	1 cut(s)	94
BsrDI	GCAATG	1 cut(s)	329
BsrFI	RCCGGY	1 cut(s)	302
BssAI	RCCGGY	1 cut(s)	302
BssECI	CCNNGG	4 cut(s)	75, 358, 1225, 1246
BssT1I	CCWWGG	1 cut(s)	1246
Bst2UI	CCWGG	3 cut(s)	872, 1071, 1226
Bst4CI	ACNGT	5 cut(s)	120, 208, 487, 647, 1146
BstDEI	CTNAG	2 cut(s)	183, 939
BstDSI	CCRYGG	1 cut(s)	358
BstEII	GGTNACC	1 cut(s)	1040
BstF5I	GGATG	4 cut(s)	513, 697, 1213, 1327
BstMAI	GTCTC	5 cut(s)	94, 126, 160, 178, 557
BstMCI	CGRYCG	2 cut(s)	18, 1261
BstMWI	GCNNNNNNNGC	8 cut(s)	23, 221, 312, 434, 480, 584, 674, 929
BstNI	CCWGG	3 cut(s)	872, 1071, 1226
BstNSI	RCATGY	2 cut(s)	677, 1132
BstPI	GGTNACC	1 cut(s)	1040
BstSCI	CCNGG	5 cut(s)	75, 285, 870, 1069, 1224
BstSFI	CTRYAG	1 cut(s)	567
BstSLI	GKGCMC	1 cut(s)	1274
BstV1I	GCAGC	2 cut(s)	412, 467
BstV2I	GAAGAC	2 cut(s)	1178, 1213
BstZI	CGGCCG	1 cut(s)	15
BsuI	GTATCC	1 cut(s)	293
BsuRI	GGCC	6 cut(s)	17, 80, 212, 443, 1124, 1272
BtgI	CCRYGG	1 cut(s)	358
BtsCI	GGATG	4 cut(s)	513, 697, 1213, 1327
BtsI	GCAGTG	1 cut(s)	381
BtsIMutI	CAGTG	5 cut(s)	116, 204, 381, 483, 1142
CciI	TCATGA	6 cut(s)	952, 982, 1000, 1033, 1063, 1234
Cfr10I	RCCGGY	1 cut(s)	302
Cfr13I	GGNCC	5 cut(s)	79, 441, 1153, 1270, 1271
Csp6I	GTAC	1 cut(s)	849
CviQI	GTAC	1 cut(s)	849
DdeI	CTNAG	2 cut(s)	183, 939
DraI	TTTAAA	1 cut(s)	1348
DrdI	GACNNNNNNGTC	1 cut(s)	650
DseDI	GACNNNNNNGTC	1 cut(s)	650
EaeI	YGGCCR	2 cut(s)	15, 210
EagI	CGGCCG	1 cut(s)	15
EclXI	CGGCCG	1 cut(s)	15
Eco130I	CCWWGG	1 cut(s)	1246
Eco24I	GRGCYC	4 cut(s)	76, 194, 317, 1274
Eco31I	GGTCTC	1 cut(s)	94
Eco32I	GATATC	1 cut(s)	1240
Eco47I	GGWCC	1 cut(s)	1153
Eco52I	CGGCCG	1 cut(s)	15
Eco57I	CTGAAG	2 cut(s)	863, 954
Eco88I	CYCGRG	1 cut(s)	193
Eco91I	GGTNACC	1 cut(s)	1040
EcoO109I	RGGNCCY	2 cut(s)	1270, 1271
EcoO65I	GGTNACC	1 cut(s)	1040
EcoRII	CCWGG	3 cut(s)	870, 1069, 1224
EcoRV	GATATC	1 cut(s)	1240
EcoT14I	CCWWGG	1 cut(s)	1246
EcoT38I	GRGCYC	4 cut(s)	76, 194, 317, 1274
ErhI	CCWWGG	1 cut(s)	1246
Esp3I	CGTCTC	1 cut(s)	178
FauI	CCCGC	2 cut(s)	113, 180
FbaI	TGATCA	4 cut(s)	547, 985, 1015, 1054
FblI	GTMKAC	1 cut(s)	402
Fnu4HI	GCNGC	6 cut(s)	27, 213, 230, 233, 426, 481
FokI	GGATG	4 cut(s)	520, 704, 1220, 1334
FriOI	GRGCYC	4 cut(s)	76, 194, 317, 1274
Fsp4HI	GCNGC	6 cut(s)	27, 213, 230, 233, 426, 481
FspBI	CTAG	1 cut(s)	23
GluI	GCNGC	6 cut(s)	27, 213, 230, 233, 426, 481
GsaI	CCCAGC	1 cut(s)	441
GsuI	CTGGAG	2 cut(s)	160, 1136
HaeIII	GGCC	6 cut(s)	17, 80, 212, 443, 1124, 1272
HapII	CCGG	6 cut(s)	77, 272, 287, 303, 318, 1156
HincII	GTYRAC	1 cut(s)	868
HindII	GTYRAC	1 cut(s)	868
HinfI	GANTC	3 cut(s)	168, 265, 785
HpaI	GTTAAC	1 cut(s)	868
HpaII	CCGG	6 cut(s)	77, 272, 287, 303, 318, 1156
HphI	GGTGA	4 cut(s)	407, 506, 986, 1034
Hpy166II	GTNNAC	4 cut(s)	204, 403, 868, 1358
Hpy188I	TCNGA	6 cut(s)	71, 250, 517, 621, 790, 893
Hpy8I	GTNNAC	4 cut(s)	204, 403, 868, 1358
Hpy99I	CGWCG	2 cut(s)	254, 295
HpyAV	CCTTC	6 cut(s)	44, 701, 937, 1094, 1114, 1333
HpyCH4III	ACNGT	5 cut(s)	120, 208, 487, 647, 1146
HpyCH4IV	ACGT	2 cut(s)	252, 656
HpyCH4V	TGCA	4 cut(s)	374, 483, 808, 1325
HpyF10VI	GCNNNNNNNGC	8 cut(s)	23, 221, 312, 434, 480, 584, 674, 929
HpyF3I	CTNAG	2 cut(s)	183, 939
HpySE526I	ACGT	2 cut(s)	252, 656
Kpn2I	TCCGGA	2 cut(s)	271, 317
Ksp22I	TGATCA	4 cut(s)	547, 985, 1015, 1054
KspAI	GTTAAC	1 cut(s)	868
LmnI	GCTCC	3 cut(s)	71, 251, 320
Lsp1109I	GCAGC	2 cut(s)	412, 467
LweI	GCATC	4 cut(s)	655, 897, 919, 1312
MaeI	CTAG	1 cut(s)	23
MaeII	ACGT	2 cut(s)	252, 656
MaeIII	GTNAC	4 cut(s)	53, 239, 652, 1040
MhlI	GDGCHC	5 cut(s)	76, 194, 317, 885, 1274
MluCI	AATT	2 cut(s)	369, 594
MlyI	GAGTC	2 cut(s)	162, 794
MmeI	TCCRAC	5 cut(s)	49, 273, 621, 1131, 1314
MroI	TCCGGA	2 cut(s)	271, 317
MseI	TTAA	6 cut(s)	336, 525, 717, 825, 867, 1347
MslI	CAYNNNNRTG	8 cut(s)	690, 999, 1002, 1023, 1035, 1050, 1056, 1062
MspA1I	CMGCKG	1 cut(s)	437
MspI	CCGG	6 cut(s)	77, 272, 287, 303, 318, 1156
MspR9I	CCNGG	5 cut(s)	77, 287, 872, 1071, 1226
MvaI	CCWGG	3 cut(s)	872, 1071, 1226
MwoI	GCNNNNNNNGC	8 cut(s)	23, 221, 312, 434, 480, 584, 674, 929
NciI	CCSGG	2 cut(s)	77, 287
NlaIV	GGNNCC	8 cut(s)	73, 316, 442, 473, 1106, 1271, 1272, 1273
NmeAIII	GCCGAG	1 cut(s)	864
NmuCI	GTSAC	4 cut(s)	53, 239, 652, 1040
NspI	RCATGY	2 cut(s)	677, 1132
PaeR7I	CTCGAG	1 cut(s)	193
PagI	TCATGA	6 cut(s)	952, 982, 1000, 1033, 1063, 1234
PciI	ACATGT	1 cut(s)	1128
PfeI	GAWTC	1 cut(s)	265
PflMI	CCANNNNNTGG	1 cut(s)	1131
PkrI	GCNGC	6 cut(s)	28, 214, 231, 234, 427, 482
Ple19I	CGATCG	1 cut(s)	1261
PleI	GAGTC	2 cut(s)	162, 793
PpsI	GAGTC	2 cut(s)	162, 793
PscI	ACATGT	1 cut(s)	1128
PshBI	ATTAAT	1 cut(s)	825
Psp6I	CCWGG	3 cut(s)	870, 1069, 1224
PspEI	GGTNACC	1 cut(s)	1040
PspFI	CCCAGC	1 cut(s)	437
PspGI	CCWGG	3 cut(s)	870, 1069, 1224
PspN4I	GGNNCC	8 cut(s)	73, 316, 442, 473, 1106, 1271, 1272, 1273
PspOMI	GGGCCC	1 cut(s)	1270
PspPI	GGNCC	5 cut(s)	79, 441, 1153, 1270, 1271
PspXI	VCTCGAGB	1 cut(s)	193
PvuI	CGATCG	1 cut(s)	1261
RsaI	GTAC	1 cut(s)	850
RsaNI	GTAC	1 cut(s)	849
RseI	CAYNNNNRTG	8 cut(s)	690, 999, 1002, 1023, 1035, 1050, 1056, 1062
SaqAI	TTAA	6 cut(s)	336, 525, 717, 825, 867, 1347
SatI	GCNGC	6 cut(s)	27, 213, 230, 233, 426, 481
Sau96I	GGNCC	5 cut(s)	79, 441, 1153, 1270, 1271
SchI	GAGTC	2 cut(s)	162, 794
ScrFI	CCNGG	5 cut(s)	77, 287, 872, 1071, 1226
SduI	GDGCHC	5 cut(s)	76, 194, 317, 885, 1274
SfaNI	GCATC	4 cut(s)	655, 897, 919, 1312
SfcI	CTRYAG	1 cut(s)	567
Sfr274I	CTCGAG	1 cut(s)	193
SinI	GGWCC	1 cut(s)	1153
SlaI	CTCGAG	1 cut(s)	193
SmiMI	CAYNNNNRTG	8 cut(s)	690, 999, 1002, 1023, 1035, 1050, 1056, 1062
SmlI	CTYRAG	1 cut(s)	193
SmoI	CTYRAG	1 cut(s)	193
Sse9I	AATT	2 cut(s)	369, 594
SsiI	CCGC	9 cut(s)	27, 106, 187, 213, 230, 233, 435, 505, 611
SspMI	CTAG	1 cut(s)	23
StyD4I	CCNGG	5 cut(s)	75, 285, 870, 1069, 1224
StyI	CCWWGG	1 cut(s)	1246
TaaI	ACNGT	5 cut(s)	120, 208, 487, 647, 1146
TaiI	ACGT	2 cut(s)	255, 659
TaqI	TCGA	3 cut(s)	194, 679, 1110
TasI	AATT	2 cut(s)	369, 594
TauI	GCSGC	4 cut(s)	29, 215, 232, 235
TfiI	GAWTC	1 cut(s)	265
Tru1I	TTAA	6 cut(s)	336, 525, 717, 825, 867, 1347
Tru9I	TTAA	6 cut(s)	336, 525, 717, 825, 867, 1347
TscAI	CASTG	5 cut(s)	123, 211, 381, 490, 1149
TseFI	GTSAC	4 cut(s)	53, 239, 652, 1040
TseI	GCWGC	2 cut(s)	425, 480
Tsp45I	GTSAC	4 cut(s)	53, 239, 652, 1040
TspDTI	ATGAA	8 cut(s)	674, 708, 1020, 1041, 1052, 1193, 1223, 1294
TspGWI	ACGGA	1 cut(s)	832
TspRI	CASTG	5 cut(s)	123, 211, 381, 490, 1149
Van91I	CCANNNNNTGG	1 cut(s)	1131
VpaK11BI	GGWCC	1 cut(s)	1153
VspI	ATTAAT	1 cut(s)	825
XceI	RCATGY	2 cut(s)	677, 1132
XhoI	CTCGAG	1 cut(s)	193
XmiI	GTMKAC	1 cut(s)	402
XspI	CTAG	1 cut(s)	23

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.