pycom16g00770

Myo-inositol oxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001948
Physical Location & Seq
Reverse (-)
461467 .. 463596
2130 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g00770.5

Sequence Viewer

Length: 921 bp
ATGACCATCCTCGTTCAGCAGCCCGACTTTGGAGCGGTTGAGGCCGAGGAGAAGGTCTCTGAATCTGGCGAGGAAAATGAGTTGGTGTTGGATGGTGGGTTTTTGGAGCCTCCGACGAACGCATTCGGCCACACTTTCAGGGATTATGATGTTAGCAGCGAGCGGCGAGATGGGGTCGAGGAGTTCTACAGAATCAATCACATTTACCAGAGCGTCGACTTTGTGAAGCGGATGAGGGAAGAATATGGAAAACTGGATAAGGTGGAAATGAGCATATGGGAATGCTGTGAACATCTCAACCAAGTTGTGGATGAGAGTGATCCCGACTTGGATGAGCCCCAGATCGAGCATCTCTTGCAGACCGCGGAAGCTATCAGAAAGGACTATCCTAATGAAGGATGGCTGCACTTGACTGCCTTGATCCATGATCTTGGCAAGGTTCTTAATCTTCCTGCTTTCGGGGAGCTTCCTCAGTGGGCTGTTGTAGGTGACACATTTCCTGTCGGGTGTGCCTTCGATGAGTCAATTGTTCATCACAAGCATTTCAAGGAAAATCCTGACTACAACAATCCTGCTTATAATACTAAGTACGGAATCTATTCTGAGGGCTGCGGACTTGACAATGTACTGATGTCATGGGGCCACGATGACTACATGTACTTGGTGGCCAAGGAGAACAAAAGCACTCTCCCTTCGGCTGGTCTTTTCATTATCAGATACCATTCATTTTATGCTTTACACAAGGCAGGAGCATATAAGCACTTGATGAATGAAGAGGACATTGAGAACTTGAAGTGGCTCAAAGTATTCAACAAGTATGACCTTTACAGCAAGAGCAAAGTTCGCATCGGTGTTGAGAAAGTCAAGCCCTACTATCTCTCTCTGATTGAGAAGTACTTTCCTGCAAAGCTGAGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko00562 map00053 map00562
Pfam Domains
Protein Families
MIOX

Protein Analysis

307

Amino Acids

35.52

Weight (kDa)

5.04

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 579
AccB7I CCANNNNNTGG 1 cut(s) 307
AccBSI CCGCTC 2 cut(s) 35, 163
AccI GTMKAC 1 cut(s) 216
AccII CGCG 1 cut(s) 365
AciI CCGC 6 cut(s) 35, 163, 229, 363, 365, 612
AclWI GGATC 2 cut(s) 314, 415
AcoI YGGCCR 2 cut(s) 127, 666
AfaI GTAC 4 cut(s) 590, 627, 659, 896
AfiI CCNNNNNNNGG 5 cut(s) 29, 307, 395, 458, 698
AflIII ACRYGT 1 cut(s) 654
AgsI TTSAA 3 cut(s) 547, 793, 811
AluBI AGCT 3 cut(s) 371, 466, 910
AluI AGCT 3 cut(s) 371, 466, 910
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 2 cut(s) 314, 415
AoxI GGCC 4 cut(s) 42, 127, 640, 666
ApeKI GCWGC 4 cut(s) 19, 156, 403, 609
Asp700I GAANNNNTTC 2 cut(s) 122, 598
AspS9I GGNCC 1 cut(s) 640
AsuHPI GGTGA 1 cut(s) 500
BalI TGGCCA 1 cut(s) 668
BanII GRGCYC 1 cut(s) 339
BbvI GCAGC 4 cut(s) 31, 168, 390, 596
BccI CCATC 5 cut(s) 14, 86, 164, 393, 909
BcoDI GTCTC 1 cut(s) 61
BfmI CTRYAG 1 cut(s) 187
BisI GCNGC 5 cut(s) 20, 157, 164, 404, 610
BlsI GCNGC 5 cut(s) 21, 158, 165, 405, 611
BmcAI AGTACT 1 cut(s) 896
BmgT120I GGNCC 1 cut(s) 640
BmiI GGNNCC 2 cut(s) 108, 641
BmsI GCATC 2 cut(s) 358, 855
BplI GAGNNNNNCTC 2 cut(s) 41, 73
BsaI GGTCTC 1 cut(s) 61
BsaJI CCNNGG 3 cut(s) 45, 363, 669
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 307, 395, 458, 698
Bse1I ACTGG 1 cut(s) 258
BseDI CCNNGG 3 cut(s) 45, 363, 669
BseGI GGATG 6 cut(s) 6, 97, 237, 316, 337, 404
BseLI CCNNNNNNNGG 5 cut(s) 29, 307, 395, 458, 698
BseMII CTCAG 3 cut(s) 485, 594, 902
BseNI ACTGG 1 cut(s) 258
BseRI GAGGAG 2 cut(s) 62, 194
BseXI GCAGC 4 cut(s) 31, 168, 390, 596
BsgI GTGCAG 1 cut(s) 389
Bsh1236I CGCG 1 cut(s) 365
BshFI GGCC 4 cut(s) 44, 129, 642, 668
BslI CCNNNNNNNGG 5 cut(s) 29, 307, 395, 458, 698
BsmAI GTCTC 1 cut(s) 61
BsmI GAATGC 2 cut(s) 122, 287
BsnI GGCC 4 cut(s) 44, 129, 642, 668
Bso31I GGTCTC 1 cut(s) 61
Bsp1286I GDGCHC 1 cut(s) 339
Bsp143I GATC 4 cut(s) 319, 342, 420, 427
BspACI CCGC 6 cut(s) 35, 163, 229, 363, 365, 612
BspANI GGCC 4 cut(s) 44, 129, 642, 668
BspCNI CTCAG 3 cut(s) 484, 595, 903
BspFNI CGCG 1 cut(s) 365
BspLI GGNNCC 2 cut(s) 108, 641
BspPI GGATC 2 cut(s) 314, 415
BspTNI GGTCTC 1 cut(s) 61
BsrBI CCGCTC 2 cut(s) 35, 163
BsrI ACTGG 1 cut(s) 258
BssECI CCNNGG 3 cut(s) 45, 363, 669
BssMI GATC 4 cut(s) 319, 342, 420, 427
BssT1I CCWWGG 1 cut(s) 669
Bst6I CTCTTC 1 cut(s) 768
BstAPI GCANNNNNTGC 1 cut(s) 355
BstC8I GCNNGC 1 cut(s) 161
BstDEI CTNAG 4 cut(s) 471, 585, 603, 911
BstDSI CCRYGG 1 cut(s) 363
BstENI CCTNNNNNAGG 1 cut(s) 393
BstF5I GGATG 6 cut(s) 6, 97, 237, 316, 337, 404
BstFNI CGCG 1 cut(s) 365
BstKTI GATC 4 cut(s) 322, 345, 423, 430
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 4 cut(s) 319, 342, 420, 427
BstMWI GCNNNNNNNGC 3 cut(s) 41, 355, 843
BstNSI RCATGY 1 cut(s) 658
BstSFI CTRYAG 1 cut(s) 187
BstUI CGCG 1 cut(s) 365
BstV1I GCAGC 4 cut(s) 31, 168, 390, 596
BstXI CCANNNNNNTGG 1 cut(s) 431
BsuRI GGCC 4 cut(s) 44, 129, 642, 668
BtgI CCRYGG 1 cut(s) 363
BtsCI GGATG 6 cut(s) 6, 97, 237, 316, 337, 404
BtsIMutI CAGTG 1 cut(s) 479
Cac8I GCNNGC 1 cut(s) 161
Cfr13I GGNCC 1 cut(s) 640
Cfr42I CCGCGG 1 cut(s) 366
CseI GACGC 1 cut(s) 202
Csp6I GTAC 4 cut(s) 589, 626, 658, 895
CviAII CATG 3 cut(s) 425, 636, 655
CviQI GTAC 4 cut(s) 589, 626, 658, 895
DdeI CTNAG 4 cut(s) 471, 585, 603, 911
DpnI GATC 4 cut(s) 321, 344, 422, 429
DpnII GATC 4 cut(s) 319, 342, 420, 427
EaeI YGGCCR 2 cut(s) 127, 666
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
Eco130I CCWWGG 1 cut(s) 669
Eco24I GRGCYC 1 cut(s) 339
Eco31I GGTCTC 1 cut(s) 61
EcoNI CCTNNNNNAGG 1 cut(s) 393
EcoT14I CCWWGG 1 cut(s) 669
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 669
FaeI CATG 3 cut(s) 428, 639, 658
FatI CATG 3 cut(s) 424, 635, 654
FauNDI CATATG 1 cut(s) 275
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 5 cut(s) 20, 157, 164, 404, 610
FokI GGATG 5 cut(s) 104, 244, 323, 344, 411
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 5 cut(s) 20, 157, 164, 404, 610
GluI GCNGC 5 cut(s) 20, 157, 164, 404, 610
HaeIII GGCC 4 cut(s) 44, 129, 642, 668
HgaI GACGC 1 cut(s) 202
Hin1II CATG 3 cut(s) 428, 639, 658
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 4 cut(s) 62, 192, 521, 594
HphI GGTGA 1 cut(s) 500
Hpy166II GTNNAC 2 cut(s) 217, 290
Hpy188I TCNGA 6 cut(s) 61, 114, 377, 604, 716, 885
Hpy188III TCNNGA 2 cut(s) 323, 557
Hpy8I GTNNAC 2 cut(s) 217, 290
Hpy99I CGWCG 2 cut(s) 118, 218
HpyAV CCTTC 4 cut(s) 46, 389, 523, 702
HpyCH4V TGCA 3 cut(s) 358, 406, 905
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 355, 843
HpyF3I CTNAG 4 cut(s) 471, 585, 603, 911
Hsp92II CATG 3 cut(s) 428, 639, 658
KspI CCGCGG 1 cut(s) 366
Kzo9I GATC 4 cut(s) 319, 342, 420, 427
LmnI GCTCC 4 cut(s) 32, 106, 463, 749
Lsp1109I GCAGC 4 cut(s) 31, 168, 390, 596
LweI GCATC 2 cut(s) 358, 855
MaeIII GTNAC 1 cut(s) 488
MalI GATC 4 cut(s) 321, 344, 422, 429
MbiI CCGCTC 2 cut(s) 35, 163
MboI GATC 4 cut(s) 319, 342, 420, 427
MboII GAAGA 3 cut(s) 251, 440, 785
MfeI CAATTG 1 cut(s) 525
MhlI GDGCHC 1 cut(s) 339
MlsI TGGCCA 1 cut(s) 668
MluCI AATT 1 cut(s) 525
MluNI TGGCCA 1 cut(s) 668
MlyI GAGTC 1 cut(s) 530
MmeI TCCRAC 2 cut(s) 69, 137
Mox20I TGGCCA 1 cut(s) 668
MroXI GAANNNNTTC 2 cut(s) 122, 598
MscI TGGCCA 1 cut(s) 668
MseI TTAA 1 cut(s) 444
Msp20I TGGCCA 1 cut(s) 668
MspA1I CMGCKG 1 cut(s) 365
MunI CAATTG 1 cut(s) 525
Mva1269I GAATGC 2 cut(s) 122, 287
MvnI CGCG 1 cut(s) 365
MwoI GCNNNNNNNGC 3 cut(s) 41, 355, 843
NdeI CATATG 1 cut(s) 275
NdeII GATC 4 cut(s) 319, 342, 420, 427
NlaIII CATG 3 cut(s) 428, 639, 658
NlaIV GGNNCC 2 cut(s) 108, 641
NmeAIII GCCGAG 1 cut(s) 70
NmuCI GTSAC 1 cut(s) 488
NspI RCATGY 1 cut(s) 658
PciI ACATGT 1 cut(s) 654
PctI GAATGC 2 cut(s) 122, 287
PdmI GAANNNNTTC 2 cut(s) 122, 598
PfeI GAWTC 3 cut(s) 62, 192, 594
PflMI CCANNNNNTGG 1 cut(s) 307
PkrI GCNGC 5 cut(s) 21, 158, 165, 405, 611
PleI GAGTC 1 cut(s) 529
PpsI GAGTC 1 cut(s) 529
PscI ACATGT 1 cut(s) 654
PsiI TTATAA 1 cut(s) 579
PspN4I GGNNCC 2 cut(s) 108, 641
PspPI GGNCC 1 cut(s) 640
RsaI GTAC 4 cut(s) 590, 627, 659, 896
RsaNI GTAC 4 cut(s) 589, 626, 658, 895
SacII CCGCGG 1 cut(s) 366
SalI GTCGAC 1 cut(s) 215
SaqAI TTAA 1 cut(s) 444
SatI GCNGC 5 cut(s) 20, 157, 164, 404, 610
Sau3AI GATC 4 cut(s) 319, 342, 420, 427
Sau96I GGNCC 1 cut(s) 640
ScaI AGTACT 1 cut(s) 896
SchI GAGTC 1 cut(s) 530
SduI GDGCHC 1 cut(s) 339
SetI ASST 8 cut(s) 57, 264, 373, 441, 468, 490, 825, 912
SfaNI GCATC 2 cut(s) 358, 855
SfcI CTRYAG 1 cut(s) 187
Sfr303I CCGCGG 1 cut(s) 366
SgrBI CCGCGG 1 cut(s) 366
Sse9I AATT 1 cut(s) 525
SsiI CCGC 6 cut(s) 35, 163, 229, 363, 365, 612
StyI CCWWGG 1 cut(s) 669
TaqI TCGA 4 cut(s) 177, 216, 345, 516
TasI AATT 1 cut(s) 525
TatI WGTACW 3 cut(s) 625, 657, 894
TauI GCSGC 1 cut(s) 166
TfiI GAWTC 3 cut(s) 62, 192, 594
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TscAI CASTG 1 cut(s) 479
TseFI GTSAC 1 cut(s) 488
TseI GCWGC 4 cut(s) 19, 156, 403, 609
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 6 cut(s) 408, 521, 697, 714, 782, 786
TspGWI ACGGA 1 cut(s) 606
TspRI CASTG 1 cut(s) 479
Van91I CCANNNNNTGG 1 cut(s) 307
XagI CCTNNNNNAGG 1 cut(s) 393
XceI RCATGY 1 cut(s) 658
XmiI GTMKAC 1 cut(s) 216
XmnI GAANNNNTTC 2 cut(s) 122, 598
ZrmI AGTACT 1 cut(s) 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.