MD17G1179700.v1.1

inositol oxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001948
Physical Location & Seq
Forward (+)
20845240 .. 20846465
1226 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1179700.v1.1.491

Sequence Viewer

Length: 444 bp
ATGGGCAGTTGTGATACATTCCCAGTTGGCTGTGCTTTTGATCAATCCAATGTTCATCACAAGTACTTTGCGGAAAATCCTGACTACTATAATCCTTCTTACAACACTAAGTATGGAGTTTACTCGGAGGGATGTGGACTCGAAAATGTTATGATGTCGTGGGGGCACGATGATTACATGTATTTGGTGGCCACAGAGAACAAATCAACTCTGCCTTCAGCAGCTCTGTTTATCATTAGATACCACTCATTCTATGCCTTACATAAAGCGGGAGCGTATAAGCACTTAATGAACGAAGAGGATGTCGAAAACCTGAAATGGCTCCGTGTATTCAACAAGTATGACCTTTACAGCAAGAGCAAGATACGGGTTGACGTTGAGAAGGTGAAGCCATACTATATTTCTCTCATTGAAAAGTACTTCCCTTCGAAGCTAAGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016701 GO:0050113 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00053 ko00562 map00053 map00562
Pfam Domains
Protein Families
MIOX

Protein Analysis

148

Amino Acids

17.34

Weight (kDa)

7.0

Isoelectric Point (pI)

55.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIOX PF05153 4 - 147 7.1e-71 Myo-inositol oxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 71, 269
AcoI YGGCCR 1 cut(s) 189
AcuI CTGAAG 1 cut(s) 201
AfaI GTAC 2 cut(s) 65, 419
AflIII ACRYGT 1 cut(s) 177
AgsI TTSAA 2 cut(s) 334, 413
AluBI AGCT 2 cut(s) 224, 433
AluI AGCT 2 cut(s) 224, 433
AoxI GGCC 1 cut(s) 189
ApeKI GCWGC 1 cut(s) 221
ArsI GACNNNNNNTTYG 2 cut(s) 288, 320
AsuHPI GGTGA 1 cut(s) 397
AsuII TTCGAA 1 cut(s) 428
BaeGI GKGCMC 1 cut(s) 168
BalI TGGCCA 1 cut(s) 191
BbvI GCAGC 1 cut(s) 233
BccI CCATC 1 cut(s) 432
BclI TGATCA 1 cut(s) 40
BisI GCNGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 223
BmcAI AGTACT 2 cut(s) 65, 419
BmiI GGNNCC 1 cut(s) 323
BmrI ACTGGG 1 cut(s) 17
BmuI ACTGGG 1 cut(s) 17
Bpu14I TTCGAA 1 cut(s) 428
Bse1I ACTGG 1 cut(s) 23
BseGI GGATG 2 cut(s) 137, 307
BseNI ACTGG 1 cut(s) 23
BseSI GKGCMC 1 cut(s) 168
BseXI GCAGC 1 cut(s) 233
BshFI GGCC 1 cut(s) 191
BsnI GGCC 1 cut(s) 191
Bsp119I TTCGAA 1 cut(s) 428
Bsp1286I GDGCHC 1 cut(s) 168
Bsp143I GATC 1 cut(s) 40
BspACI CCGC 2 cut(s) 71, 269
BspANI GGCC 1 cut(s) 191
BspLI GGNNCC 1 cut(s) 323
BspT104I TTCGAA 1 cut(s) 428
BsrI ACTGG 1 cut(s) 23
BssMI GATC 1 cut(s) 40
Bst6I CTCTTC 1 cut(s) 291
BstBI TTCGAA 1 cut(s) 428
BstDEI CTNAG 2 cut(s) 108, 434
BstF5I GGATG 2 cut(s) 137, 307
BstKTI GATC 1 cut(s) 43
BstMBI GATC 1 cut(s) 40
BstNSI RCATGY 1 cut(s) 181
BstSLI GKGCMC 1 cut(s) 168
BstV1I GCAGC 1 cut(s) 233
BsuRI GGCC 1 cut(s) 191
BtsCI GGATG 2 cut(s) 137, 307
Csp6I GTAC 2 cut(s) 64, 418
CviAII CATG 1 cut(s) 178
CviJI RGCY 6 cut(s) 30, 191, 224, 322, 391, 433
CviKI_1 RGCY 6 cut(s) 30, 191, 224, 322, 391, 433
CviQI GTAC 2 cut(s) 64, 418
DdeI CTNAG 2 cut(s) 108, 434
DpnI GATC 1 cut(s) 42
DpnII GATC 1 cut(s) 40
EaeI YGGCCR 1 cut(s) 189
Eam1104I CTCTTC 1 cut(s) 291
EarI CTCTTC 1 cut(s) 291
Eco57I CTGAAG 1 cut(s) 201
FaeI CATG 1 cut(s) 181
FatI CATG 1 cut(s) 177
FauI CCCGC 1 cut(s) 262
FbaI TGATCA 1 cut(s) 40
Fnu4HI GCNGC 1 cut(s) 222
FokI GGATG 2 cut(s) 144, 314
Fsp4HI GCNGC 1 cut(s) 222
GluI GCNGC 1 cut(s) 222
HaeIII GGCC 1 cut(s) 191
Hin1II CATG 1 cut(s) 181
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HinfI GANTC 1 cut(s) 138
HphI GGTGA 1 cut(s) 397
Hpy166II GTNNAC 3 cut(s) 121, 137, 373
Hpy188I TCNGA 1 cut(s) 127
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 3 cut(s) 121, 137, 373
HpyAV CCTTC 4 cut(s) 105, 225, 376, 435
HpyCH4IV ACGT 1 cut(s) 375
HpyF3I CTNAG 2 cut(s) 108, 434
HpySE526I ACGT 1 cut(s) 375
Hsp92II CATG 1 cut(s) 181
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 1 cut(s) 40
LmnI GCTCC 2 cut(s) 272, 327
LpnPI CCDG 3 cut(s) 36, 93, 326
Lsp1109I GCAGC 1 cut(s) 233
MaeII ACGT 1 cut(s) 375
MalI GATC 1 cut(s) 42
MboI GATC 1 cut(s) 40
MboII GAAGA 1 cut(s) 308
MhlI GDGCHC 1 cut(s) 168
MlsI TGGCCA 1 cut(s) 191
MluNI TGGCCA 1 cut(s) 191
MlyI GAGTC 1 cut(s) 132
MnlI CCTC 2 cut(s) 121, 292
Mox20I TGGCCA 1 cut(s) 191
MscI TGGCCA 1 cut(s) 191
MseI TTAA 1 cut(s) 287
Msp20I TGGCCA 1 cut(s) 191
NdeII GATC 1 cut(s) 40
NlaIII CATG 1 cut(s) 181
NlaIV GGNNCC 1 cut(s) 323
NspI RCATGY 1 cut(s) 181
NspV TTCGAA 1 cut(s) 428
PciI ACATGT 1 cut(s) 177
PkrI GCNGC 1 cut(s) 223
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
PscI ACATGT 1 cut(s) 177
PspN4I GGNNCC 1 cut(s) 323
RsaI GTAC 2 cut(s) 65, 419
RsaNI GTAC 2 cut(s) 64, 418
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 1 cut(s) 222
Sau3AI GATC 1 cut(s) 40
ScaI AGTACT 2 cut(s) 65, 419
SchI GAGTC 1 cut(s) 132
SduI GDGCHC 1 cut(s) 168
SetI ASST 6 cut(s) 226, 315, 348, 378, 387, 435
SfuI TTCGAA 1 cut(s) 428
SsiI CCGC 2 cut(s) 71, 269
TaiI ACGT 1 cut(s) 378
TaqI TCGA 3 cut(s) 141, 306, 428
TatI WGTACW 2 cut(s) 63, 417
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TseI GCWGC 1 cut(s) 221
TspDTI ATGAA 2 cut(s) 44, 305
TspGWI ACGGA 1 cut(s) 314
XceI RCATGY 1 cut(s) 181
ZrmI AGTACT 2 cut(s) 65, 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.