FvH4_5g03570

WUSCHEL-related homeobox 8-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002233
Physical Location & Seq
Reverse (-)
2093367 .. 2094123
757 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g03570.t1

Sequence Viewer

Length: 489 bp
ATGGAAGGGCAGAGAAGAGTGTTGGCAATGAAATTGGAGAGGCCGAGGACTCAGAACCAAGATGGGTTTTGCGTCATGACGGAAGACCAAGTCGAGCAACTCCGGAAACAGATCGCCGTCTACGCCGTCATCTGTGAGCAGCTTGTTGATACGCATAAGGCCTTCACTGCCCAACAGGATCTTGCTGGAATGAAGATGGGAAATGTGTATGGCGATTCGATGGCATTTGGTGGCAACAAGGTAACTGCGAGGCAGCGGTGGACGCCATCAAATACGCAACTTGAAATCCTTGAGCGTATCTATAATGAAGCCAACGGAACTCCAGGAAAGCAGAGAGTTAAAGAGATAACCAAGGAACTCTCACAACATGGCCAAGTTGCTGAAACTAATGTTTACAATTGGTTCCAAAACAGAAGAGCTCGTTCAAAGAGAAAGCAATCGGTGCCAACACGGATCAATACAGAATCAGATTGTATCTCCCAAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Homeobox

Protein Analysis

163

Amino Acids

18.54

Weight (kDa)

9.4

Isoelectric Point (pI)

47.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 84 - 144 2.2e-14 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 442
AccI GTMKAC 1 cut(s) 120
AccIII TCCGGA 1 cut(s) 102
AciI CCGC 1 cut(s) 256
AclWI GGATC 2 cut(s) 186, 461
AcoI YGGCCR 1 cut(s) 370
AcyI GRCGYC 1 cut(s) 263
AgsI TTSAA 2 cut(s) 284, 426
AjnI CCWGG 1 cut(s) 322
AluBI AGCT 2 cut(s) 142, 419
AluI AGCT 2 cut(s) 142, 419
Alw21I GWGCWC 1 cut(s) 421
AlwI GGATC 2 cut(s) 186, 461
Aor13HI TCCGGA 1 cut(s) 102
AoxI GGCC 3 cut(s) 41, 159, 370
ApeKI GCWGC 2 cut(s) 139, 253
BalI TGGCCA 1 cut(s) 372
BanI GGYRCC 1 cut(s) 442
BanII GRGCYC 1 cut(s) 421
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 421
BbvI GCAGC 2 cut(s) 151, 265
BccI CCATC 4 cut(s) 56, 190, 214, 274
BceAI ACGGC 2 cut(s) 101, 110
BciT130I CCWGG 1 cut(s) 324
BisI GCNGC 2 cut(s) 140, 254
BlsI GCNGC 2 cut(s) 141, 255
Bme1390I CCNGG 1 cut(s) 324
BmiI GGNNCC 2 cut(s) 404, 444
BmrFI CCNGG 1 cut(s) 324
BpiI GAAGAC 1 cut(s) 90
BpmI CTGGAG 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 311
BsaHI GRCGYC 1 cut(s) 263
BsaJI CCNNGG 3 cut(s) 44, 351, 481
BsaWI WCCGGW 1 cut(s) 102
Bse3DI GCAATG 1 cut(s) 33
BseAI TCCGGA 1 cut(s) 102
BseBI CCWGG 1 cut(s) 324
BseDI CCNNGG 3 cut(s) 44, 351, 481
BseMI GCAATG 1 cut(s) 33
BseMII CTCAG 1 cut(s) 65
BseXI GCAGC 2 cut(s) 151, 265
BshFI GGCC 3 cut(s) 43, 161, 372
BshNI GGYRCC 1 cut(s) 442
BsiHKAI GWGCWC 1 cut(s) 421
BsiSI CCGG 1 cut(s) 103
BsnI GGCC 3 cut(s) 43, 161, 372
Bsp1286I GDGCHC 1 cut(s) 421
Bsp13I TCCGGA 1 cut(s) 102
Bsp143I GATC 3 cut(s) 111, 178, 453
BspACI CCGC 1 cut(s) 256
BspANI GGCC 3 cut(s) 43, 161, 372
BspCNI CTCAG 1 cut(s) 64
BspEI TCCGGA 1 cut(s) 102
BspHI TCATGA 1 cut(s) 75
BspLI GGNNCC 2 cut(s) 404, 444
BspPI GGATC 2 cut(s) 186, 461
BspQI GCTCTTC 1 cut(s) 409
BspT107I GGYRCC 1 cut(s) 442
BsrDI GCAATG 1 cut(s) 33
BssECI CCNNGG 3 cut(s) 44, 351, 481
BssMI GATC 3 cut(s) 111, 178, 453
BssNI GRCGYC 1 cut(s) 263
BssT1I CCWWGG 2 cut(s) 351, 481
Bst2UI CCWGG 1 cut(s) 324
Bst6I CTCTTC 2 cut(s) 10, 409
BstACI GRCGYC 1 cut(s) 263
BstAPI GCANNNNNTGC 1 cut(s) 442
BstDEI CTNAG 1 cut(s) 51
BstKTI GATC 3 cut(s) 114, 181, 456
BstMBI GATC 3 cut(s) 111, 178, 453
BstMWI GCNNNNNNNGC 4 cut(s) 122, 167, 262, 442
BstNI CCWGG 1 cut(s) 324
BstSCI CCNGG 1 cut(s) 322
BstV1I GCAGC 2 cut(s) 151, 265
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 1 cut(s) 178
BstYI RGATCY 1 cut(s) 178
BsuRI GGCC 3 cut(s) 43, 161, 372
BtsI GCAGTG 1 cut(s) 165
BtsIMutI CAGTG 1 cut(s) 165
CciI TCATGA 1 cut(s) 75
CseI GACGC 2 cut(s) 61, 271
CviAII CATG 2 cut(s) 76, 368
CviJI RGCY 6 cut(s) 43, 142, 161, 311, 372, 419
CviKI_1 RGCY 6 cut(s) 43, 142, 161, 311, 372, 419
DdeI CTNAG 1 cut(s) 51
DpnI GATC 3 cut(s) 113, 180, 455
DpnII GATC 3 cut(s) 111, 178, 453
EaeI YGGCCR 1 cut(s) 370
Eam1104I CTCTTC 2 cut(s) 10, 409
EarI CTCTTC 2 cut(s) 10, 409
Ecl136II GAGCTC 1 cut(s) 419
Eco130I CCWWGG 2 cut(s) 351, 481
Eco147I AGGCCT 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 421
Eco53kI GAGCTC 1 cut(s) 419
EcoICRI GAGCTC 1 cut(s) 419
EcoRII CCWGG 1 cut(s) 322
EcoT14I CCWWGG 2 cut(s) 351, 481
EcoT38I GRGCYC 1 cut(s) 421
ErhI CCWWGG 2 cut(s) 351, 481
FaeI CATG 2 cut(s) 79, 371
FaiI YATR 5 cut(s) 77, 156, 210, 303, 369
FatI CATG 2 cut(s) 75, 367
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 2 cut(s) 140, 254
FriOI GRGCYC 1 cut(s) 421
Fsp4HI GCNGC 2 cut(s) 140, 254
GluI GCNGC 2 cut(s) 140, 254
GsuI CTGGAG 1 cut(s) 306
HaeIII GGCC 3 cut(s) 43, 161, 372
HapII CCGG 1 cut(s) 103
HgaI GACGC 2 cut(s) 61, 271
Hin1I GRCGYC 1 cut(s) 263
Hin1II CATG 2 cut(s) 79, 371
HinfI GANTC 3 cut(s) 49, 215, 464
HpaII CCGG 1 cut(s) 103
Hpy166II GTNNAC 3 cut(s) 121, 261, 394
Hpy188I TCNGA 2 cut(s) 54, 469
Hpy188III TCNNGA 2 cut(s) 76, 103
Hpy8I GTNNAC 3 cut(s) 121, 261, 394
HpyAV CCTTC 1 cut(s) 172
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 167, 262, 442
HpyF3I CTNAG 1 cut(s) 51
Hsp92I GRCGYC 1 cut(s) 263
Hsp92II CATG 2 cut(s) 79, 371
Kpn2I TCCGGA 1 cut(s) 102
Kzo9I GATC 3 cut(s) 111, 178, 453
LguI GCTCTTC 1 cut(s) 409
LpnPI CCDG 5 cut(s) 116, 161, 171, 309, 336
Lsp1109I GCAGC 2 cut(s) 151, 265
MaeIII GTNAC 1 cut(s) 241
MalI GATC 3 cut(s) 113, 180, 455
MboI GATC 3 cut(s) 111, 178, 453
MboII GAAGA 4 cut(s) 27, 95, 205, 426
MfeI CAATTG 1 cut(s) 397
MflI RGATCY 1 cut(s) 178
MhlI GDGCHC 1 cut(s) 421
MlsI TGGCCA 1 cut(s) 372
MluCI AATT 2 cut(s) 32, 397
MluNI TGGCCA 1 cut(s) 372
MlyI GAGTC 1 cut(s) 43
MnlI CCTC 3 cut(s) 33, 39, 243
Mox20I TGGCCA 1 cut(s) 372
MroI TCCGGA 1 cut(s) 102
MscI TGGCCA 1 cut(s) 372
MseI TTAA 1 cut(s) 339
Msp20I TGGCCA 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 256
MspI CCGG 1 cut(s) 103
MspR9I CCNGG 1 cut(s) 324
MunI CAATTG 1 cut(s) 397
MvaI CCWGG 1 cut(s) 324
MwoI GCNNNNNNNGC 4 cut(s) 122, 167, 262, 442
NdeII GATC 3 cut(s) 111, 178, 453
NlaIII CATG 2 cut(s) 79, 371
NlaIV GGNNCC 2 cut(s) 404, 444
NmeAIII GCCGAG 1 cut(s) 69
PagI TCATGA 1 cut(s) 75
PceI AGGCCT 1 cut(s) 161
PciSI GCTCTTC 1 cut(s) 409
PfeI GAWTC 2 cut(s) 215, 464
PflFI GACNNNGTC 1 cut(s) 89
PfoI TCCNGGA 1 cut(s) 322
PkrI GCNGC 2 cut(s) 141, 255
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
Psp124BI GAGCTC 1 cut(s) 421
Psp6I CCWGG 1 cut(s) 322
PspGI CCWGG 1 cut(s) 322
PspN4I GGNNCC 2 cut(s) 404, 444
PsuI RGATCY 1 cut(s) 178
PsyI GACNNNGTC 1 cut(s) 89
SacI GAGCTC 1 cut(s) 421
SapI GCTCTTC 1 cut(s) 409
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 2 cut(s) 140, 254
Sau3AI GATC 3 cut(s) 111, 178, 453
SchI GAGTC 1 cut(s) 43
ScrFI CCNGG 1 cut(s) 324
SduI GDGCHC 1 cut(s) 421
SetI ASST 3 cut(s) 144, 243, 421
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 2 cut(s) 32, 397
SseBI AGGCCT 1 cut(s) 161
SsiI CCGC 1 cut(s) 256
SstI GAGCTC 1 cut(s) 421
StuI AGGCCT 1 cut(s) 161
StyD4I CCNGG 1 cut(s) 322
StyI CCWWGG 2 cut(s) 351, 481
TaqI TCGA 2 cut(s) 93, 218
TasI AATT 2 cut(s) 32, 397
TfiI GAWTC 2 cut(s) 215, 464
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 1 cut(s) 172
TseI GCWGC 2 cut(s) 139, 253
TspDTI ATGAA 3 cut(s) 44, 206, 321
TspGWI ACGGA 3 cut(s) 95, 330, 466
TspRI CASTG 1 cut(s) 172
Tth111I GACNNNGTC 1 cut(s) 89
XmiI GTMKAC 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.