Rroxscaffold_2G00144960

WUSCHEL-related homeobox

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002233
Physical Location & Seq
Forward (+)
82828200 .. 82830855
2656 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00144960.1

Sequence Viewer

Length: 795 bp
ATGGGGAATGGGAATGGAGGAGTGGGGTTTGTGAAGGTTATGACTGATGAGCAATTGGAGACTCTCAGAAAGCAAATTGCTGTTTATGCTACCATTTGTGAGCAACTTGTGGAGATGCATAAGAACCTCACTGCCCAGCAAGATCTTGCAGGAGTCAGGATGGGTAATCTGTACTGTGATCCACTGATGACATCTGTTGGCCACAAAATCACCGCCAGACAGCGGTGGACTCCAACACCGGTGCAGCTACAGATTCTGGAGCAATTGTTTGATCAAGGGAATGGAACTCCAAGCAAACAAAAGATCAAGGAGATAACCTCTGAACTGAGCCAGCACGGTCAAATTTCTGAAACCAATGTTTACAATTGGTTCCAGAATAGGCGTGCTCGATCAAAGAGGAAGCAACAGAATGCAACATCCAACAATGCTGAATCAGAAGTGGAGACTGAAGTTGACTCTCCCAAGGACAAGAAAAAAGGACCTCAGGATCAACTGTTACAGTCCCAACAGAACACATCAGCTCAAAGGGCTGAAGACTTGTGCTTTCAGAACCCTGAGATGAGTTCTGACCTGCATTTCTTGGATCCACAGGGCATTAAAGAAGACAAAATGTTTGCATCCAGCAGCGATTTAAGGCCTTCCAGAGGTTTAGGCCATATGTCATACTATGATGAAGTGCTCTCAAGCTCAAGAGGAAATGAGCTGATGACAGGAAAGATGGAAGTGGGGAGGGGAGCTATAGTCTTTTCCAGCAGGCAGAAGACTACAGCATGTGACAAGTTATATTTTTTATGA

Protein Analysis

264

Amino Acids

29.69

Weight (kDa)

6.97

Isoelectric Point (pI)

56.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 73 - 133 1.4e-16 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 579
AciI CCGC 2 cut(s) 213, 223
AclWI GGATC 4 cut(s) 173, 495, 578, 591
AcoI YGGCCR 1 cut(s) 199
AcsI RAATTY 1 cut(s) 342
AcuI CTGAAG 2 cut(s) 468, 552
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 2 cut(s) 222, 644
AgeI ACCGGT 1 cut(s) 238
AluBI AGCT 5 cut(s) 247, 521, 687, 703, 737
AluI AGCT 5 cut(s) 247, 521, 687, 703, 737
Alw21I GWGCWC 2 cut(s) 388, 681
Alw26I GTCTC 2 cut(s) 53, 437
AlwI GGATC 4 cut(s) 173, 495, 578, 591
AlwNI CAGNNNCTG 1 cut(s) 256
AoxI GGCC 3 cut(s) 199, 635, 652
ApeKI GCWGC 2 cut(s) 244, 624
ApoI RAATTY 1 cut(s) 342
ArsI GACNNNNNNTTYG 2 cut(s) 596, 628
AsiGI ACCGGT 1 cut(s) 238
AspS9I GGNCC 1 cut(s) 479
AsuHPI GGTGA 1 cut(s) 202
AvaII GGWCC 1 cut(s) 479
AxyI CCTNAGG 1 cut(s) 483
BalI TGGCCA 1 cut(s) 201
BamHI GGATCC 1 cut(s) 583
BbsI GAAGAC 3 cut(s) 540, 609, 767
Bbv12I GWGCWC 2 cut(s) 388, 681
BbvI GCAGC 2 cut(s) 256, 636
BccI CCATC 2 cut(s) 154, 712
BclI TGATCA 1 cut(s) 271
BcoDI GTCTC 2 cut(s) 53, 437
BfmI CTRYAG 3 cut(s) 248, 738, 765
BfuAI ACCTGC 1 cut(s) 579
BglII AGATCT 1 cut(s) 142
BisI GCNGC 2 cut(s) 245, 625
BlsI GCNGC 2 cut(s) 246, 626
Bme18I GGWCC 1 cut(s) 479
BmgT120I GGNCC 1 cut(s) 479
BmiI GGNNCC 2 cut(s) 371, 585
BmsI GCATC 2 cut(s) 105, 626
BpiI GAAGAC 3 cut(s) 540, 609, 767
BplI GAGNNNNNCTC 2 cut(s) 302, 334
BpmI CTGGAG 1 cut(s) 278
BpuEI CTTGAG 2 cut(s) 667, 673
BsaJI CCNNGG 1 cut(s) 462
BsaWI WCCGGW 1 cut(s) 238
BsaXI ACNNNNNCTCC 4 cut(s) 251, 281, 726, 756
Bsc4I CCNNNNNNNGG 2 cut(s) 222, 644
Bse118I RCCGGY 1 cut(s) 238
Bse21I CCTNAGG 1 cut(s) 483
BseDI CCNNGG 1 cut(s) 462
BseGI GGATG 3 cut(s) 165, 416, 617
BseLI CCNNNNNNNGG 2 cut(s) 222, 644
BseMII CTCAG 4 cut(s) 79, 317, 497, 546
BseRI GAGGAG 1 cut(s) 33
BseXI GCAGC 2 cut(s) 256, 636
BseYI CCCAGC 1 cut(s) 135
BsgI GTGCAG 1 cut(s) 263
BshFI GGCC 3 cut(s) 201, 637, 654
BshTI ACCGGT 1 cut(s) 238
BsiHKAI GWGCWC 2 cut(s) 388, 681
BsiSI CCGG 1 cut(s) 239
BslFI GGGAC 1 cut(s) 487
BslI CCNNNNNNNGG 2 cut(s) 222, 644
BsmAI GTCTC 2 cut(s) 53, 437
BsmFI GGGAC 1 cut(s) 487
BsmI GAATGC 1 cut(s) 415
BsnI GGCC 3 cut(s) 201, 637, 654
Bsp1286I GDGCHC 2 cut(s) 388, 681
Bsp143I GATC 7 cut(s) 142, 178, 271, 303, 389, 487, 583
BspACI CCGC 2 cut(s) 213, 223
BspANI GGCC 3 cut(s) 201, 637, 654
BspCNI CTCAG 4 cut(s) 78, 318, 496, 547
BspLI GGNNCC 2 cut(s) 371, 585
BspMI ACCTGC 1 cut(s) 579
BspPI GGATC 4 cut(s) 173, 495, 578, 591
BsrFI RCCGGY 1 cut(s) 238
BssAI RCCGGY 1 cut(s) 238
BssECI CCNNGG 1 cut(s) 462
BssMI GATC 7 cut(s) 142, 178, 271, 303, 389, 487, 583
BssT1I CCWWGG 1 cut(s) 462
Bst4CI ACNGT 4 cut(s) 176, 338, 495, 501
BstC8I GCNNGC 3 cut(s) 332, 384, 755
BstDEI CTNAG 4 cut(s) 65, 326, 483, 555
BstENI CCTNNNNNAGG 1 cut(s) 642
BstF5I GGATG 3 cut(s) 165, 416, 617
BstKTI GATC 7 cut(s) 145, 181, 274, 306, 392, 490, 586
BstMAI GTCTC 2 cut(s) 53, 437
BstMBI GATC 7 cut(s) 142, 178, 271, 303, 389, 487, 583
BstMWI GCNNNNNNNGC 2 cut(s) 86, 527
BstNSI RCATGY 1 cut(s) 774
BstSFI CTRYAG 3 cut(s) 248, 738, 765
BstV1I GCAGC 2 cut(s) 256, 636
BstV2I GAAGAC 3 cut(s) 540, 609, 767
BstX2I RGATCY 2 cut(s) 142, 583
BstYI RGATCY 2 cut(s) 142, 583
Bsu36I CCTNAGG 1 cut(s) 483
BsuRI GGCC 3 cut(s) 201, 637, 654
BtsCI GGATG 3 cut(s) 165, 416, 617
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 2 cut(s) 129, 182
BveI ACCTGC 1 cut(s) 579
Cac8I GCNNGC 3 cut(s) 332, 384, 755
CaiI CAGNNNCTG 1 cut(s) 256
Cfr10I RCCGGY 1 cut(s) 238
Cfr13I GGNCC 1 cut(s) 479
Csp6I GTAC 1 cut(s) 172
CspAI ACCGGT 1 cut(s) 238
CviAII CATG 1 cut(s) 771
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 4 cut(s) 65, 326, 483, 555
DpnI GATC 7 cut(s) 144, 180, 273, 305, 391, 489, 585
DpnII GATC 7 cut(s) 142, 178, 271, 303, 389, 487, 583
EaeI YGGCCR 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 462
Eco147I AGGCCT 1 cut(s) 637
Eco47I GGWCC 1 cut(s) 479
Eco57I CTGAAG 2 cut(s) 468, 552
Eco81I CCTNAGG 1 cut(s) 483
EcoNI CCTNNNNNAGG 1 cut(s) 642
EcoO109I RGGNCCY 1 cut(s) 479
EcoT14I CCWWGG 1 cut(s) 462
EcoT22I ATGCAT 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 462
FaeI CATG 1 cut(s) 774
FaqI GGGAC 1 cut(s) 487
FatI CATG 1 cut(s) 770
FauNDI CATATG 1 cut(s) 657
FbaI TGATCA 1 cut(s) 271
Fnu4HI GCNGC 2 cut(s) 245, 625
FokI GGATG 3 cut(s) 172, 403, 604
Fsp4HI GCNGC 2 cut(s) 245, 625
GluI GCNGC 2 cut(s) 245, 625
GsaI CCCAGC 1 cut(s) 139
GsuI CTGGAG 1 cut(s) 278
HaeIII GGCC 3 cut(s) 201, 637, 654
HapII CCGG 1 cut(s) 239
Hin1II CATG 1 cut(s) 774
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HinfI GANTC 6 cut(s) 61, 153, 229, 253, 431, 455
HpaII CCGG 1 cut(s) 239
HphI GGTGA 1 cut(s) 202
Hpy166II GTNNAC 3 cut(s) 228, 361, 454
Hpy188I TCNGA 6 cut(s) 68, 322, 349, 436, 549, 568
Hpy188III TCNNGA 6 cut(s) 157, 257, 373, 485, 642, 690
Hpy8I GTNNAC 3 cut(s) 228, 361, 454
HpyAV CCTTC 2 cut(s) 28, 648
HpyCH4III ACNGT 4 cut(s) 176, 338, 495, 501
HpyCH4V TGCA 6 cut(s) 118, 149, 244, 413, 574, 617
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 527
HpyF3I CTNAG 4 cut(s) 65, 326, 483, 555
Hsp92II CATG 1 cut(s) 774
Ksp22I TGATCA 1 cut(s) 271
Kzo9I GATC 7 cut(s) 142, 178, 271, 303, 389, 487, 583
LmnI GCTCC 2 cut(s) 259, 734
Lsp1109I GCAGC 2 cut(s) 256, 636
LweI GCATC 2 cut(s) 105, 626
MaeIII GTNAC 2 cut(s) 495, 773
MalI GATC 7 cut(s) 144, 180, 273, 305, 391, 489, 585
MboI GATC 7 cut(s) 142, 178, 271, 303, 389, 487, 583
MboII GAAGA 3 cut(s) 545, 614, 772
MfeI CAATTG 3 cut(s) 53, 263, 364
MflI RGATCY 2 cut(s) 142, 583
MhlI GDGCHC 2 cut(s) 388, 681
MlsI TGGCCA 1 cut(s) 201
MluCI AATT 5 cut(s) 53, 75, 263, 342, 364
MluNI TGGCCA 1 cut(s) 201
MlyI GAGTC 4 cut(s) 55, 162, 223, 449
MmeI TCCRAC 2 cut(s) 257, 444
MnlI CCTC 8 cut(s) 11, 137, 328, 390, 492, 638, 686, 723
Mox20I TGGCCA 1 cut(s) 201
Mph1103I ATGCAT 1 cut(s) 120
MscI TGGCCA 1 cut(s) 201
MseI TTAA 2 cut(s) 597, 632
Msp20I TGGCCA 1 cut(s) 201
MspA1I CMGCKG 1 cut(s) 223
MspI CCGG 1 cut(s) 239
MunI CAATTG 3 cut(s) 53, 263, 364
Mva1269I GAATGC 1 cut(s) 415
MwoI GCNNNNNNNGC 2 cut(s) 86, 527
NdeI CATATG 1 cut(s) 657
NdeII GATC 7 cut(s) 142, 178, 271, 303, 389, 487, 583
NlaIII CATG 1 cut(s) 774
NlaIV GGNNCC 2 cut(s) 371, 585
NmuCI GTSAC 1 cut(s) 773
NsiI ATGCAT 1 cut(s) 120
NspI RCATGY 1 cut(s) 774
PceI AGGCCT 1 cut(s) 637
PctI GAATGC 1 cut(s) 415
PfeI GAWTC 2 cut(s) 253, 431
PinAI ACCGGT 1 cut(s) 238
PkrI GCNGC 2 cut(s) 246, 626
PleI GAGTC 4 cut(s) 55, 161, 223, 449
PpsI GAGTC 4 cut(s) 55, 161, 223, 449
PpuMI RGGWCCY 1 cut(s) 479
Psp5II RGGWCCY 1 cut(s) 479
PspFI CCCAGC 1 cut(s) 135
PspN4I GGNNCC 2 cut(s) 371, 585
PspPI GGNCC 1 cut(s) 479
PspPPI RGGWCCY 1 cut(s) 479
PstNI CAGNNNCTG 1 cut(s) 256
PsuI RGATCY 2 cut(s) 142, 583
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
SaqAI TTAA 2 cut(s) 597, 632
SatI GCNGC 2 cut(s) 245, 625
Sau3AI GATC 7 cut(s) 142, 178, 271, 303, 389, 487, 583
Sau96I GGNCC 1 cut(s) 479
SchI GAGTC 4 cut(s) 55, 162, 223, 449
SduI GDGCHC 2 cut(s) 388, 681
SfaNI GCATC 2 cut(s) 105, 626
SfcI CTRYAG 3 cut(s) 248, 738, 765
SgrAI CRCCGGYG 1 cut(s) 238
SinI GGWCC 1 cut(s) 479
SmlI CTYRAG 2 cut(s) 682, 688
SmoI CTYRAG 2 cut(s) 682, 688
Sse9I AATT 5 cut(s) 53, 75, 263, 342, 364
SseBI AGGCCT 1 cut(s) 637
SsiI CCGC 2 cut(s) 213, 223
StuI AGGCCT 1 cut(s) 637
StyI CCWWGG 1 cut(s) 462
TaaI ACNGT 4 cut(s) 176, 338, 495, 501
TaqI TCGA 1 cut(s) 388
TasI AATT 5 cut(s) 53, 75, 263, 342, 364
TatI WGTACW 1 cut(s) 171
TfiI GAWTC 2 cut(s) 253, 431
Tru1I TTAA 2 cut(s) 597, 632
Tru9I TTAA 2 cut(s) 597, 632
TscAI CASTG 2 cut(s) 136, 189
TseFI GTSAC 1 cut(s) 773
TseI GCWGC 2 cut(s) 244, 624
Tsp45I GTSAC 1 cut(s) 773
TspDTI ATGAA 1 cut(s) 687
TspRI CASTG 2 cut(s) 136, 189
VpaK11BI GGWCC 1 cut(s) 479
XagI CCTNNNNNAGG 1 cut(s) 642
XapI RAATTY 1 cut(s) 342
XceI RCATGY 1 cut(s) 774
Zsp2I ATGCAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.