MD00G1207300.v1.1

WUSCHEL-related homeobox 8-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002233
Physical Location & Seq
Reverse (-)
50458386 .. 50461175
2790 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1207300.v1.1.491

Sequence Viewer

Length: 648 bp
ATGATGGGGGAGTGGCGGCAGCAGCAGCAGCAAATAATCAACCAGAATTTGCAGAACCCAGAAGAAGATGATGGGGTTGGAAATGGAAATGGAGGGTTGTTCGTGAAGGTTATGACCGACGAGCAAATGGAGCAGCTGAGGCAACAGATCGCCGTCTACACCACCATTTGTGACCAGCTTGTCGACTTGCACAAGCAACTCTCTGCCCAACAGGATCTTACTGGAATGAGACTGGGGAATTTTTATTGTGATCCATTCATGGCATCCGGTGGCCACAAGATAACTTCAAGGCAGCGCTGGACCCCGTCACCCGTGCAACTTCAAATCCTTGAGCAAATTTTTGAGGAAGGCAACGGAACTCCATGCAAGCAGAAGATCAAAGAGCTAACCATGGAACTCACACAGCATGGCCAGATATCTGAAACAAATGTCTACAATTGGTTCCAGAACAGGAGAGCTCGTTCAAAAAGAAAGCAATCCATGACCGCGCAAAACAATGCAGAATCAGAAGCAGAGCCAGAAGTTGAATCTCCCAGGGATGAAAAGACAAAACCAGAAGACATCTTGTCCTTTGACAACTCTGCACTGAGGGCTGAAAATATGTACCTTCAAAGCCCGGAATTAGGATTTGGAAATTTTGATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Homeobox

Protein Analysis

216

Amino Acids

24.75

Weight (kDa)

4.85

Isoelectric Point (pI)

61.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 97 - 157 5.5e-16 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 179
AccI GTMKAC 3 cut(s) 156, 183, 432
AccII CGCG 1 cut(s) 488
AciI CCGC 2 cut(s) 16, 486
AclWI GGATC 2 cut(s) 222, 245
AcoI YGGCCR 2 cut(s) 271, 409
AcsI RAATTY 4 cut(s) 46, 238, 336, 634
AfaI GTAC 1 cut(s) 605
AfeI AGCGCT 1 cut(s) 296
AfiI CCNNNNNNNGG 1 cut(s) 623
AgsI TTSAA 5 cut(s) 288, 323, 465, 527, 611
AhdI GACNNNNNGTC 1 cut(s) 565
AjnI CCWGG 1 cut(s) 533
AjuI GAANNNNNNNTTGG 3 cut(s) 612, 625, 644
AloI GAACNNNNNNTCC 2 cut(s) 445, 477
AluBI AGCT 4 cut(s) 136, 178, 385, 458
AluI AGCT 4 cut(s) 136, 178, 385, 458
Alw21I GWGCWC 1 cut(s) 460
Alw26I GTCTC 1 cut(s) 223
AlwI GGATC 2 cut(s) 222, 245
Aor51HI AGCGCT 1 cut(s) 296
AoxI GGCC 2 cut(s) 271, 409
ApeKI GCWGC 6 cut(s) 19, 22, 25, 28, 133, 292
ApoI RAATTY 4 cut(s) 46, 238, 336, 634
AspLEI GCGC 2 cut(s) 297, 490
AspS9I GGNCC 1 cut(s) 300
AsuC2I CCSGG 1 cut(s) 617
AsuHPI GGTGA 1 cut(s) 300
AvaII GGWCC 1 cut(s) 300
BalI TGGCCA 2 cut(s) 273, 411
BanII GRGCYC 1 cut(s) 460
BbsI GAAGAC 1 cut(s) 564
Bbv12I GWGCWC 1 cut(s) 460
BbvCI CCTCAGC 1 cut(s) 137
BbvI GCAGC 6 cut(s) 31, 34, 37, 40, 145, 304
BccI CCATC 1 cut(s) 65
BceAI ACGGC 1 cut(s) 137
BciT130I CCWGG 1 cut(s) 535
BcnI CCSGG 1 cut(s) 617
BcoDI GTCTC 1 cut(s) 223
BfoI RGCGCY 1 cut(s) 298
BisI GCNGC 7 cut(s) 17, 20, 23, 26, 29, 134, 293
BlsI GCNGC 7 cut(s) 18, 21, 24, 27, 30, 135, 294
Bme1390I CCNGG 2 cut(s) 535, 617
Bme18I GGWCC 1 cut(s) 300
BmeRI GACNNNNNGTC 1 cut(s) 565
BmgT120I GGNCC 1 cut(s) 300
BmiI GGNNCC 2 cut(s) 302, 443
BmrFI CCNGG 2 cut(s) 535, 617
BmrI ACTGGG 1 cut(s) 242
BmsI GCATC 1 cut(s) 272
BmuI ACTGGG 1 cut(s) 242
BpiI GAAGAC 1 cut(s) 564
Bpu10I CCTNAGC 1 cut(s) 137
BpuEI CTTGAG 1 cut(s) 350
BpuMI CCSGG 1 cut(s) 617
BsaJI CCNNGG 3 cut(s) 390, 533, 534
BsaWI WCCGGW 1 cut(s) 266
BsaXI ACNNNNNCTCC 2 cut(s) 445, 475
Bsc4I CCNNNNNNNGG 1 cut(s) 623
Bse1I ACTGG 2 cut(s) 226, 237
BseBI CCWGG 1 cut(s) 535
BseDI CCNNGG 3 cut(s) 390, 533, 534
BseGI GGATG 2 cut(s) 263, 544
BseLI CCNNNNNNNGG 1 cut(s) 623
BseMII CTCAG 2 cut(s) 128, 578
BseNI ACTGG 2 cut(s) 226, 237
BseXI GCAGC 6 cut(s) 31, 34, 37, 40, 145, 304
BsgI GTGCAG 1 cut(s) 567
Bsh1236I CGCG 1 cut(s) 488
BshFI GGCC 2 cut(s) 273, 411
BsiHKAI GWGCWC 1 cut(s) 460
BsiSI CCGG 2 cut(s) 267, 617
BslI CCNNNNNNNGG 1 cut(s) 623
BsmAI GTCTC 1 cut(s) 223
BsnI GGCC 2 cut(s) 273, 411
Bsp1286I GDGCHC 1 cut(s) 460
Bsp143I GATC 4 cut(s) 147, 214, 250, 375
Bsp19I CCATGG 1 cut(s) 390
BspACI CCGC 2 cut(s) 16, 486
BspANI GGCC 2 cut(s) 273, 411
BspCNI CTCAG 2 cut(s) 129, 579
BspFNI CGCG 1 cut(s) 488
BspLI GGNNCC 2 cut(s) 302, 443
BspPI GGATC 2 cut(s) 222, 245
BsrI ACTGG 2 cut(s) 226, 237
BssECI CCNNGG 3 cut(s) 390, 533, 534
BssMI GATC 4 cut(s) 147, 214, 250, 375
BssT1I CCWWGG 1 cut(s) 390
Bst2UI CCWGG 1 cut(s) 535
BstC8I GCNNGC 1 cut(s) 368
BstDEI CTNAG 2 cut(s) 137, 587
BstDSI CCRYGG 1 cut(s) 390
BstF5I GGATG 2 cut(s) 263, 544
BstFNI CGCG 1 cut(s) 488
BstH2I RGCGCY 1 cut(s) 298
BstHHI GCGC 2 cut(s) 297, 490
BstKTI GATC 4 cut(s) 150, 217, 253, 378
BstMAI GTCTC 1 cut(s) 223
BstMBI GATC 4 cut(s) 147, 214, 250, 375
BstMWI GCNNNNNNNGC 6 cut(s) 22, 25, 28, 130, 139, 590
BstNI CCWGG 1 cut(s) 535
BstSCI CCNGG 2 cut(s) 533, 615
BstUI CGCG 1 cut(s) 488
BstV1I GCAGC 6 cut(s) 31, 34, 37, 40, 145, 304
BstV2I GAAGAC 1 cut(s) 564
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
BsuRI GGCC 2 cut(s) 273, 411
BtgI CCRYGG 1 cut(s) 390
BtsCI GGATG 2 cut(s) 263, 544
BtsIMutI CAGTG 1 cut(s) 584
Cac8I GCNNGC 1 cut(s) 368
CfoI GCGC 2 cut(s) 297, 490
Cfr13I GGNCC 1 cut(s) 300
Csp6I GTAC 1 cut(s) 604
CviAII CATG 5 cut(s) 259, 363, 391, 407, 481
CviJI RGCY 9 cut(s) 136, 178, 273, 385, 411, 458, 517, 593, 615
CviKI_1 RGCY 9 cut(s) 136, 178, 273, 385, 411, 458, 517, 593, 615
CviQI GTAC 1 cut(s) 604
DdeI CTNAG 2 cut(s) 137, 587
DpnI GATC 4 cut(s) 149, 216, 252, 377
DpnII GATC 4 cut(s) 147, 214, 250, 375
DrdI GACNNNNNNGTC 1 cut(s) 179
DriI GACNNNNNGTC 1 cut(s) 565
DseDI GACNNNNNNGTC 1 cut(s) 179
EaeI YGGCCR 2 cut(s) 271, 409
Eam1105I GACNNNNNGTC 1 cut(s) 565
Ecl136II GAGCTC 1 cut(s) 458
Eco130I CCWWGG 1 cut(s) 390
Eco24I GRGCYC 1 cut(s) 460
Eco32I GATATC 1 cut(s) 417
Eco47I GGWCC 1 cut(s) 300
Eco47III AGCGCT 1 cut(s) 296
Eco53kI GAGCTC 1 cut(s) 458
EcoICRI GAGCTC 1 cut(s) 458
EcoRII CCWGG 1 cut(s) 533
EcoRV GATATC 1 cut(s) 417
EcoT14I CCWWGG 1 cut(s) 390
EcoT38I GRGCYC 1 cut(s) 460
ErhI CCWWGG 1 cut(s) 390
FaeI CATG 5 cut(s) 262, 366, 394, 410, 484
FaiI YATR 7 cut(s) 113, 260, 364, 392, 408, 482, 602
FatI CATG 5 cut(s) 258, 362, 390, 406, 480
FblI GTMKAC 3 cut(s) 156, 183, 432
Fnu4HI GCNGC 7 cut(s) 17, 20, 23, 26, 29, 134, 293
FokI GGATG 2 cut(s) 250, 551
FriOI GRGCYC 1 cut(s) 460
Fsp4HI GCNGC 7 cut(s) 17, 20, 23, 26, 29, 134, 293
GlaI GCGC 2 cut(s) 296, 489
GluI GCNGC 7 cut(s) 17, 20, 23, 26, 29, 134, 293
HaeII RGCGCY 1 cut(s) 298
HaeIII GGCC 2 cut(s) 273, 411
HapII CCGG 2 cut(s) 267, 617
HhaI GCGC 2 cut(s) 297, 490
Hin1II CATG 5 cut(s) 262, 366, 394, 410, 484
Hin6I GCGC 2 cut(s) 295, 488
HinP1I GCGC 2 cut(s) 295, 488
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HinfI GANTC 2 cut(s) 503, 527
HpaII CCGG 2 cut(s) 267, 617
HphI GGTGA 1 cut(s) 300
Hpy166II GTNNAC 3 cut(s) 157, 184, 433
Hpy188I TCNGA 2 cut(s) 421, 508
Hpy188III TCNNGA 2 cut(s) 103, 445
Hpy8I GTNNAC 3 cut(s) 157, 184, 433
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 3 cut(s) 100, 341, 617
HpyCH4V TGCA 6 cut(s) 52, 190, 316, 366, 500, 584
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 25, 28, 130, 139, 590
HpyF3I CTNAG 2 cut(s) 137, 587
Hsp92II CATG 5 cut(s) 262, 366, 394, 410, 484
HspAI GCGC 2 cut(s) 295, 488
Kzo9I GATC 4 cut(s) 147, 214, 250, 375
LmnI GCTCC 1 cut(s) 130
Lsp1109I GCAGC 6 cut(s) 31, 34, 37, 40, 145, 304
LweI GCATC 1 cut(s) 272
MaeIII GTNAC 2 cut(s) 170, 306
MalI GATC 4 cut(s) 149, 216, 252, 377
MboI GATC 4 cut(s) 147, 214, 250, 375
MboII GAAGA 4 cut(s) 74, 77, 385, 569
MfeI CAATTG 1 cut(s) 436
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 1 cut(s) 460
MlsI TGGCCA 2 cut(s) 273, 411
MluCI AATT 6 cut(s) 46, 238, 336, 436, 620, 634
MluNI TGGCCA 2 cut(s) 273, 411
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 4 cut(s) 86, 132, 337, 582
Mox20I TGGCCA 2 cut(s) 273, 411
MscI TGGCCA 2 cut(s) 273, 411
Msp20I TGGCCA 2 cut(s) 273, 411
MspA1I CMGCKG 1 cut(s) 136
MspI CCGG 2 cut(s) 267, 617
MspR9I CCNGG 2 cut(s) 535, 617
MunI CAATTG 1 cut(s) 436
MvaI CCWGG 1 cut(s) 535
MvnI CGCG 1 cut(s) 488
MwoI GCNNNNNNNGC 6 cut(s) 22, 25, 28, 130, 139, 590
NciI CCSGG 1 cut(s) 617
NcoI CCATGG 1 cut(s) 390
NdeII GATC 4 cut(s) 147, 214, 250, 375
NlaIII CATG 5 cut(s) 262, 366, 394, 410, 484
NlaIV GGNNCC 2 cut(s) 302, 443
NmuCI GTSAC 2 cut(s) 170, 306
PasI CCCWGGG 1 cut(s) 534
PfeI GAWTC 2 cut(s) 503, 527
PflFI GACNNNGTC 1 cut(s) 304
PkrI GCNGC 7 cut(s) 18, 21, 24, 27, 30, 135, 294
Psp124BI GAGCTC 1 cut(s) 460
Psp6I CCWGG 1 cut(s) 533
PspGI CCWGG 1 cut(s) 533
PspN4I GGNNCC 2 cut(s) 302, 443
PspPI GGNCC 1 cut(s) 300
PsuI RGATCY 1 cut(s) 214
PsyI GACNNNGTC 1 cut(s) 304
PvuII CAGCTG 1 cut(s) 136
RsaI GTAC 1 cut(s) 605
RsaNI GTAC 1 cut(s) 604
SacI GAGCTC 1 cut(s) 460
SalI GTCGAC 1 cut(s) 182
SatI GCNGC 7 cut(s) 17, 20, 23, 26, 29, 134, 293
Sau3AI GATC 4 cut(s) 147, 214, 250, 375
Sau96I GGNCC 1 cut(s) 300
ScrFI CCNGG 2 cut(s) 535, 617
SduI GDGCHC 1 cut(s) 460
SetI ASST 6 cut(s) 111, 138, 180, 387, 460, 609
SfaNI GCATC 1 cut(s) 272
SinI GGWCC 1 cut(s) 300
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 6 cut(s) 46, 238, 336, 436, 620, 634
SsiI CCGC 2 cut(s) 16, 486
SstI GAGCTC 1 cut(s) 460
StyD4I CCNGG 2 cut(s) 533, 615
StyI CCWWGG 1 cut(s) 390
TaqI TCGA 1 cut(s) 183
TaqII GACCGA 1 cut(s) 131
TasI AATT 6 cut(s) 46, 238, 336, 436, 620, 634
TauI GCSGC 1 cut(s) 19
TfiI GAWTC 2 cut(s) 503, 527
TscAI CASTG 1 cut(s) 591
TseFI GTSAC 2 cut(s) 170, 306
TseI GCWGC 6 cut(s) 19, 22, 25, 28, 133, 292
Tsp45I GTSAC 2 cut(s) 170, 306
TspDTI ATGAA 2 cut(s) 247, 555
TspGWI ACGGA 1 cut(s) 369
TspRI CASTG 1 cut(s) 591
Tth111I GACNNNGTC 1 cut(s) 304
VpaK11BI GGWCC 1 cut(s) 300
XapI RAATTY 4 cut(s) 46, 238, 336, 634
XmiI GTMKAC 3 cut(s) 156, 183, 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.