Rorug07G0156200

WUSCHEL-related homeobox 8-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0002233
Physical Location & Seq
Reverse (-)
12433611 .. 12435093
1483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0156200.1

Sequence Viewer

Length: 915 bp
ATGGAGAACTGGGATTTGCAAGCTGTGGTGAGAGGGTGCAAAAACAATCTTGAAGGCCTTACTCATACCATGGAGTTCTCTCAATCATCTTGTTTTGGCTTTGCTCCTGTAACCATTGAAGAAGATGATGAGATTTTCCAGGCTTTTCCTCAACTGTTTGAAGCCACAACAGCTTTGGATGAATTGGAGGAGCTTTACAAGCCCTTCTACCCAATTTTGCAGCCTATTTCCCCACCAACCATTCCTACCGCCTCCGTATCGGTTCCGCAAGAAGTCACTGCAGAAGAAAAAGAGCCAAAGAAGCTGAAAAGCGTGAAAGAATCCTCACCCATGTCCAAAAGAAGGAAGAATCAGAGCAAGAGGGTGGTGCAAGAGGTTCAGGAGAAAGATCTGTTCTCGGATGTGTGGGCGTGGCGAAAATATGGGCAAAAACCCATCAAGGGATCGCCATATCCGAGGAGCTATTACCGGTGCAGTAGCTCGAAAGGATGTTCAGCAAGAAAGCAAGTTGAAAGGAGCAATTCCAATCCAGAATTGTTCATCATAACCTACACTGCAGAACACAACCATGTTCATCCTACTCGGCGAAACTCTCTTGCTGGAAGCACAAGAACCAAGTTCCCCAAATCGCAGAAAAAAGAAAAGAGCAAAAATGAGTTTGTGCCTGTAAAAGAGGAAGCACAGAGACTGCCGGAAGAGGATGAAGTTAGTCAAGAGAGTGTGATGGATATGATGTTGGGAGATGAAGTTGTTCCGGGCTTTGAGGATTTTGATCAAAAGTTGGAGGAATTGAGCGAATTGATGGCGGAACCAGTCACGGCCGATGGTTTCTTTTCGGATCAGTTTCCAGATAATTATTTTCCAAGTTCTTGGTTCCTTGGTCACTCTACAAGTACTGTAACTGGAGCTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Homeobox

Protein Analysis

304

Amino Acids

34.65

Weight (kDa)

5.15

Isoelectric Point (pI)

62.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 134 - 190 1.5e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 249, 266, 806
AclWI GGATC 2 cut(s) 451, 846
AcoI YGGCCR 1 cut(s) 819
AfaI GTAC 1 cut(s) 895
AfiI CCNNNNNNNGG 2 cut(s) 342, 440
AgeI ACCGGT 1 cut(s) 468
AgsI TTSAA 4 cut(s) 53, 119, 161, 512
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 7 cut(s) 23, 173, 193, 304, 462, 480, 908
AluI AGCT 7 cut(s) 23, 173, 193, 304, 462, 480, 908
Alw26I GTCTC 1 cut(s) 679
AlwI GGATC 2 cut(s) 451, 846
AlwNI CAGNNNCTG 1 cut(s) 688
AoxI GGCC 2 cut(s) 55, 819
ApeKI GCWGC 1 cut(s) 220
AsiGI ACCGGT 1 cut(s) 468
Asp700I GAANNNNTTC 1 cut(s) 750
AsuC2I CCSGG 1 cut(s) 756
AsuHPI GGTGA 2 cut(s) 40, 318
BbvI GCAGC 1 cut(s) 232
BccI CCATC 4 cut(s) 443, 718, 796, 818
BceAI ACGGC 1 cut(s) 834
BciT130I CCWGG 1 cut(s) 140
BclI TGATCA 1 cut(s) 772
BcnI CCSGG 1 cut(s) 756
BcoDI GTCTC 1 cut(s) 679
BfmI CTRYAG 2 cut(s) 279, 555
BglII AGATCT 1 cut(s) 388
BisI GCNGC 1 cut(s) 221
BlsI GCNGC 1 cut(s) 222
BmcAI AGTACT 1 cut(s) 895
Bme1390I CCNGG 2 cut(s) 140, 756
BmiI GGNNCC 3 cut(s) 264, 810, 875
BmrFI CCNGG 2 cut(s) 140, 756
BmrI ACTGGG 1 cut(s) 19
BmuI ACTGGG 1 cut(s) 19
BpuMI CCSGG 1 cut(s) 756
BsaBI GATNNNNATC 1 cut(s) 771
BsaJI CCNNGG 3 cut(s) 69, 455, 877
BsaWI WCCGGW 1 cut(s) 468
BsaXI ACNNNNNCTCC 2 cut(s) 732, 762
Bsc4I CCNNNNNNNGG 2 cut(s) 342, 440
Bse118I RCCGGY 1 cut(s) 468
Bse1I ACTGG 3 cut(s) 14, 812, 907
Bse8I GATNNNNATC 1 cut(s) 771
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 3 cut(s) 69, 455, 877
BseGI GGATG 5 cut(s) 184, 406, 494, 574, 706
BseJI GATNNNNATC 1 cut(s) 771
BseLI CCNNNNNNNGG 2 cut(s) 342, 440
BseNI ACTGG 3 cut(s) 14, 812, 907
BseRI GAGGAG 2 cut(s) 203, 472
BseX3I CGGCCG 1 cut(s) 819
BseXI GCAGC 1 cut(s) 232
BsgI GTGCAG 1 cut(s) 493
Bsh1285I CGRYCG 1 cut(s) 822
BshFI GGCC 2 cut(s) 57, 821
BshTI ACCGGT 1 cut(s) 468
BsiEI CGRYCG 1 cut(s) 822
BsiSI CCGG 3 cut(s) 469, 692, 755
BslI CCNNNNNNNGG 2 cut(s) 342, 440
BsmAI GTCTC 1 cut(s) 679
BsnI GGCC 2 cut(s) 57, 821
Bsp143I GATC 4 cut(s) 388, 443, 772, 838
Bsp19I CCATGG 1 cut(s) 69
BspACI CCGC 3 cut(s) 249, 266, 806
BspANI GGCC 2 cut(s) 57, 821
BspLI GGNNCC 3 cut(s) 264, 810, 875
BspMAI CTGCAG 2 cut(s) 283, 559
BspPI GGATC 2 cut(s) 451, 846
BsrFI RCCGGY 1 cut(s) 468
BsrI ACTGG 3 cut(s) 14, 812, 907
BssAI RCCGGY 1 cut(s) 468
BssECI CCNNGG 3 cut(s) 69, 455, 877
BssMI GATC 4 cut(s) 388, 443, 772, 838
BssT1I CCWWGG 2 cut(s) 69, 877
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 2 cut(s) 156, 898
Bst6I CTCTTC 1 cut(s) 690
BstC8I GCNNGC 2 cut(s) 21, 910
BstDSI CCRYGG 1 cut(s) 69
BstF5I GGATG 5 cut(s) 184, 406, 494, 574, 706
BstKTI GATC 4 cut(s) 391, 446, 775, 841
BstMAI GTCTC 1 cut(s) 679
BstMBI GATC 4 cut(s) 388, 443, 772, 838
BstMCI CGRYCG 1 cut(s) 822
BstMWI GCNNNNNNNGC 3 cut(s) 170, 199, 301
BstNI CCWGG 1 cut(s) 140
BstSCI CCNGG 2 cut(s) 138, 754
BstSFI CTRYAG 2 cut(s) 279, 555
BstV1I GCAGC 1 cut(s) 232
BstX2I RGATCY 1 cut(s) 388
BstXI CCANNNNNNTGG 1 cut(s) 870
BstYI RGATCY 1 cut(s) 388
BstZI CGGCCG 1 cut(s) 819
BsuRI GGCC 2 cut(s) 57, 821
BtgI CCRYGG 1 cut(s) 69
BtsCI GGATG 5 cut(s) 184, 406, 494, 574, 706
BtsI GCAGTG 2 cut(s) 276, 552
BtsIMutI CAGTG 2 cut(s) 276, 552
Cac8I GCNNGC 2 cut(s) 21, 910
CaiI CAGNNNCTG 1 cut(s) 688
Cfr10I RCCGGY 1 cut(s) 468
Csp6I GTAC 1 cut(s) 894
CspAI ACCGGT 1 cut(s) 468
CviAII CATG 3 cut(s) 70, 331, 569
CviQI GTAC 1 cut(s) 894
DpnI GATC 4 cut(s) 390, 445, 774, 840
DpnII GATC 4 cut(s) 388, 443, 772, 838
EaeI YGGCCR 1 cut(s) 819
EagI CGGCCG 1 cut(s) 819
Eam1104I CTCTTC 1 cut(s) 690
EarI CTCTTC 1 cut(s) 690
EciI GGCGGA 1 cut(s) 821
EclXI CGGCCG 1 cut(s) 819
Eco130I CCWWGG 2 cut(s) 69, 877
Eco147I AGGCCT 1 cut(s) 57
Eco52I CGGCCG 1 cut(s) 819
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 2 cut(s) 69, 877
ErhI CCWWGG 2 cut(s) 69, 877
FaeI CATG 3 cut(s) 73, 334, 572
FaiI YATR 8 cut(s) 66, 71, 332, 423, 451, 545, 570, 731
FatI CATG 3 cut(s) 69, 330, 568
FbaI TGATCA 1 cut(s) 772
Fnu4HI GCNGC 1 cut(s) 221
FokI GGATG 5 cut(s) 191, 413, 501, 561, 713
Fsp4HI GCNGC 1 cut(s) 221
GluI GCNGC 1 cut(s) 221
HaeIII GGCC 2 cut(s) 57, 821
HapII CCGG 3 cut(s) 469, 692, 755
Hin1II CATG 3 cut(s) 73, 334, 572
HinfI GANTC 2 cut(s) 320, 349
HpaII CCGG 3 cut(s) 469, 692, 755
HphI GGTGA 2 cut(s) 40, 318
Hpy188I TCNGA 4 cut(s) 354, 400, 456, 838
Hpy188III TCNNGA 5 cut(s) 50, 380, 530, 713, 848
HpyAV CCTTC 3 cut(s) 47, 214, 336
HpyCH4III ACNGT 2 cut(s) 156, 898
HpyCH4V TGCA 7 cut(s) 19, 39, 220, 281, 370, 474, 557
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 199, 301
Hsp92II CATG 3 cut(s) 73, 334, 572
Ksp22I TGATCA 1 cut(s) 772
Kzo9I GATC 4 cut(s) 388, 443, 772, 838
LmnI GCTCC 5 cut(s) 109, 190, 459, 516, 905
Lsp1109I GCAGC 1 cut(s) 232
MaeIII GTNAC 5 cut(s) 109, 274, 814, 881, 898
MalI GATC 4 cut(s) 390, 445, 774, 840
MboI GATC 4 cut(s) 388, 443, 772, 838
MboII GAAGA 5 cut(s) 131, 134, 296, 358, 707
MflI RGATCY 1 cut(s) 388
MluCI AATT 7 cut(s) 182, 213, 520, 533, 788, 797, 853
MmeI TCCRAC 1 cut(s) 762
MroXI GAANNNNTTC 1 cut(s) 750
MslI CAYNNNNRTG 1 cut(s) 567
MspI CCGG 3 cut(s) 469, 692, 755
MspR9I CCNGG 2 cut(s) 140, 756
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 3 cut(s) 170, 199, 301
NciI CCSGG 1 cut(s) 756
NcoI CCATGG 1 cut(s) 69
NdeII GATC 4 cut(s) 388, 443, 772, 838
NlaIII CATG 3 cut(s) 73, 334, 572
NlaIV GGNNCC 3 cut(s) 264, 810, 875
NmeAIII GCCGAG 1 cut(s) 562
NmuCI GTSAC 3 cut(s) 274, 814, 881
PceI AGGCCT 1 cut(s) 57
PcsI WCGNNNNNNNCGW 1 cut(s) 452
PdmI GAANNNNTTC 1 cut(s) 750
PfeI GAWTC 2 cut(s) 320, 349
PinAI ACCGGT 1 cut(s) 468
PkrI GCNGC 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 3 cut(s) 264, 810, 875
PstI CTGCAG 2 cut(s) 283, 559
PstNI CAGNNNCTG 1 cut(s) 688
PsuI RGATCY 1 cut(s) 388
RsaI GTAC 1 cut(s) 895
RsaNI GTAC 1 cut(s) 894
RseI CAYNNNNRTG 1 cut(s) 567
SatI GCNGC 1 cut(s) 221
Sau3AI GATC 4 cut(s) 388, 443, 772, 838
ScaI AGTACT 1 cut(s) 895
ScrFI CCNGG 2 cut(s) 140, 756
SetI ASST 9 cut(s) 25, 175, 195, 306, 378, 464, 482, 551, 910
SfcI CTRYAG 2 cut(s) 279, 555
SmiMI CAYNNNNRTG 1 cut(s) 567
Sse9I AATT 7 cut(s) 182, 213, 520, 533, 788, 797, 853
SseBI AGGCCT 1 cut(s) 57
SsiI CCGC 3 cut(s) 249, 266, 806
StuI AGGCCT 1 cut(s) 57
StyD4I CCNGG 2 cut(s) 138, 754
StyI CCWWGG 2 cut(s) 69, 877
TaaI ACNGT 2 cut(s) 156, 898
TaqI TCGA 1 cut(s) 482
TasI AATT 7 cut(s) 182, 213, 520, 533, 788, 797, 853
TatI WGTACW 1 cut(s) 893
TfiI GAWTC 2 cut(s) 320, 349
TscAI CASTG 2 cut(s) 283, 559
TseFI GTSAC 3 cut(s) 274, 814, 881
TseI GCWGC 1 cut(s) 220
Tsp45I GTSAC 3 cut(s) 274, 814, 881
TspDTI ATGAA 5 cut(s) 195, 529, 563, 717, 759
TspGWI ACGGA 1 cut(s) 244
TspRI CASTG 2 cut(s) 283, 559
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XmnI GAANNNNTTC 1 cut(s) 750
ZrmI AGTACT 1 cut(s) 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.