FvH4_5g25470

WUSCHEL-related homeobox 8-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002233
Physical Location & Seq
Forward (+)
16950096 .. 16952492
2397 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g25470.t1

Sequence Viewer

Length: 600 bp
ATGGAAAATGGGTTTCTAAGTAAGGTGAGAATGGAGGGGCAGAGAAGAGTGTTGGCAGTGAAATTGGAGAGGCTGAGGACTCAGAACCCAGATGGGTTTTGCGTCATGACGGACGACCAAGTCGAGCAACTCCAGAAACAGATCGCCGTCTACGCCGTCATCTGTGAGCAGCTTGTTGATATGCACAAGGCCTTCACTGCCCAACAGGATCTTGCTGGAATGAAGATGGGAAATGTGTACGGCGATTTGATGCCATTTGGTGGCAACAAGGTAACTGCAAGGCAGCGGTGGACGCCATCAAATACGCAACTTGAAATCCTTGAGCGTATCTATAATGAAGCCAACGGAACTCCAGGAAAGCACAGAGTCAAAGAGATAACCAAGGAACTCTCACAACATGGCCATGTTACTGAAACTAATGTTTACAATTGGTTCCAAAACAGAAGAGCTCGTTCAAAGAGAAAGCAATCGGTGCCAACACGGAACATGACAGAATTAGATATAGAGCCCGAGGTTATATCTCCCAAGGATAAGAAGACAAAACCTGAAGACATGTACTTTCGAAGTCCTGATTCAGGAAATGAGAACCCCAAATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Homeobox

Protein Analysis

200

Amino Acids

23.04

Weight (kDa)

9.18

Isoelectric Point (pI)

54.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 94 - 154 8.9e-15 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 472
AccB7I CCANNNNNTGG 1 cut(s) 260
AccI GTMKAC 1 cut(s) 150
AciI CCGC 1 cut(s) 286
AclWI GGATC 1 cut(s) 216
AcoI YGGCCR 1 cut(s) 400
AcuI CTGAAG 1 cut(s) 567
AcyI GRCGYC 1 cut(s) 293
AfaI GTAC 2 cut(s) 239, 557
AfiI CCNNNNNNNGG 2 cut(s) 260, 575
AflIII ACRYGT 1 cut(s) 552
AgsI TTSAA 2 cut(s) 314, 456
AjnI CCWGG 1 cut(s) 352
AluBI AGCT 2 cut(s) 172, 449
AluI AGCT 2 cut(s) 172, 449
Alw21I GWGCWC 1 cut(s) 451
AlwI GGATC 1 cut(s) 216
Ama87I CYCGRG 1 cut(s) 509
AoxI GGCC 2 cut(s) 189, 400
ApeKI GCWGC 2 cut(s) 169, 283
AsuHPI GGTGA 1 cut(s) 37
AsuII TTCGAA 1 cut(s) 562
AvaI CYCGRG 1 cut(s) 509
BalI TGGCCA 1 cut(s) 402
BanI GGYRCC 1 cut(s) 472
BanII GRGCYC 2 cut(s) 451, 510
BbsI GAAGAC 2 cut(s) 542, 555
Bbv12I GWGCWC 1 cut(s) 451
BbvCI CCTCAGC 1 cut(s) 74
BbvI GCAGC 2 cut(s) 181, 295
BccI CCATC 3 cut(s) 86, 220, 304
BceAI ACGGC 3 cut(s) 131, 140, 256
BciT130I CCWGG 1 cut(s) 354
BisI GCNGC 2 cut(s) 170, 284
BlsI GCNGC 2 cut(s) 171, 285
Bme1390I CCNGG 1 cut(s) 354
BmeT110I CYCGRG 1 cut(s) 509
BmiI GGNNCC 2 cut(s) 434, 474
BmrFI CCNGG 1 cut(s) 354
BmsI GCATC 1 cut(s) 240
BpiI GAAGAC 2 cut(s) 542, 555
BpmI CTGGAG 2 cut(s) 116, 336
Bpu10I CCTNAGC 1 cut(s) 74
Bpu14I TTCGAA 1 cut(s) 562
BpuEI CTTGAG 1 cut(s) 341
BsaHI GRCGYC 1 cut(s) 293
BsaJI CCNNGG 3 cut(s) 381, 510, 525
Bsc4I CCNNNNNNNGG 2 cut(s) 260, 575
BseBI CCWGG 1 cut(s) 354
BseDI CCNNGG 3 cut(s) 381, 510, 525
BseLI CCNNNNNNNGG 2 cut(s) 260, 575
BseMII CTCAG 2 cut(s) 65, 95
BseXI GCAGC 2 cut(s) 181, 295
BshFI GGCC 2 cut(s) 191, 402
BshNI GGYRCC 1 cut(s) 472
BsiHKAI GWGCWC 1 cut(s) 451
BsiHKCI CYCGRG 1 cut(s) 509
BslI CCNNNNNNNGG 2 cut(s) 260, 575
BsnI GGCC 2 cut(s) 191, 402
BsoBI CYCGRG 1 cut(s) 509
Bsp119I TTCGAA 1 cut(s) 562
Bsp1286I GDGCHC 2 cut(s) 451, 510
Bsp143I GATC 2 cut(s) 141, 208
BspACI CCGC 1 cut(s) 286
BspANI GGCC 2 cut(s) 191, 402
BspCNI CTCAG 2 cut(s) 66, 94
BspHI TCATGA 1 cut(s) 105
BspLI GGNNCC 2 cut(s) 434, 474
BspPI GGATC 1 cut(s) 216
BspQI GCTCTTC 1 cut(s) 439
BspT104I TTCGAA 1 cut(s) 562
BspT107I GGYRCC 1 cut(s) 472
BssECI CCNNGG 3 cut(s) 381, 510, 525
BssMI GATC 2 cut(s) 141, 208
BssNI GRCGYC 1 cut(s) 293
BssT1I CCWWGG 2 cut(s) 381, 525
Bst2UI CCWGG 1 cut(s) 354
Bst6I CTCTTC 2 cut(s) 40, 439
BstACI GRCGYC 1 cut(s) 293
BstAPI GCANNNNNTGC 1 cut(s) 472
BstBI TTCGAA 1 cut(s) 562
BstDEI CTNAG 3 cut(s) 17, 74, 81
BstENI CCTNNNNNAGG 1 cut(s) 573
BstKTI GATC 2 cut(s) 144, 211
BstMBI GATC 2 cut(s) 141, 208
BstMWI GCNNNNNNNGC 4 cut(s) 152, 197, 292, 472
BstNI CCWGG 1 cut(s) 354
BstNSI RCATGY 1 cut(s) 556
BstSCI CCNGG 1 cut(s) 352
BstV1I GCAGC 2 cut(s) 181, 295
BstV2I GAAGAC 2 cut(s) 542, 555
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
BsuRI GGCC 2 cut(s) 191, 402
BtsI GCAGTG 2 cut(s) 63, 195
BtsIMutI CAGTG 2 cut(s) 63, 195
CciI TCATGA 1 cut(s) 105
CseI GACGC 2 cut(s) 91, 301
Csp6I GTAC 2 cut(s) 238, 556
CviAII CATG 5 cut(s) 106, 398, 404, 487, 553
CviJI RGCY 7 cut(s) 73, 172, 191, 341, 402, 449, 508
CviKI_1 RGCY 7 cut(s) 73, 172, 191, 341, 402, 449, 508
CviQI GTAC 2 cut(s) 238, 556
DdeI CTNAG 3 cut(s) 17, 74, 81
DpnI GATC 2 cut(s) 143, 210
DpnII GATC 2 cut(s) 141, 208
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 400
Eam1104I CTCTTC 2 cut(s) 40, 439
EarI CTCTTC 2 cut(s) 40, 439
Ecl136II GAGCTC 1 cut(s) 449
Eco130I CCWWGG 2 cut(s) 381, 525
Eco147I AGGCCT 1 cut(s) 191
Eco24I GRGCYC 2 cut(s) 451, 510
Eco53kI GAGCTC 1 cut(s) 449
Eco57I CTGAAG 1 cut(s) 567
Eco88I CYCGRG 1 cut(s) 509
EcoICRI GAGCTC 1 cut(s) 449
EcoNI CCTNNNNNAGG 1 cut(s) 573
EcoRII CCWGG 1 cut(s) 352
EcoT14I CCWWGG 2 cut(s) 381, 525
EcoT38I GRGCYC 2 cut(s) 451, 510
ErhI CCWWGG 2 cut(s) 381, 525
FaeI CATG 5 cut(s) 109, 401, 407, 490, 556
FaiI YATR 9 cut(s) 107, 182, 333, 399, 405, 488, 503, 518, 554
FatI CATG 5 cut(s) 105, 397, 403, 486, 552
FblI GTMKAC 1 cut(s) 150
Fnu4HI GCNGC 2 cut(s) 170, 284
FriOI GRGCYC 2 cut(s) 451, 510
Fsp4HI GCNGC 2 cut(s) 170, 284
GluI GCNGC 2 cut(s) 170, 284
GsuI CTGGAG 2 cut(s) 116, 336
HaeIII GGCC 2 cut(s) 191, 402
HgaI GACGC 2 cut(s) 91, 301
Hin1I GRCGYC 1 cut(s) 293
Hin1II CATG 5 cut(s) 109, 401, 407, 490, 556
HinfI GANTC 3 cut(s) 79, 366, 572
HphI GGTGA 1 cut(s) 37
Hpy166II GTNNAC 4 cut(s) 151, 238, 291, 424
Hpy188I TCNGA 1 cut(s) 84
Hpy188III TCNNGA 4 cut(s) 106, 133, 569, 576
Hpy8I GTNNAC 4 cut(s) 151, 238, 291, 424
HpyAV CCTTC 1 cut(s) 202
HpyCH4V TGCA 2 cut(s) 184, 278
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 197, 292, 472
HpyF3I CTNAG 3 cut(s) 17, 74, 81
Hsp92I GRCGYC 1 cut(s) 293
Hsp92II CATG 5 cut(s) 109, 401, 407, 490, 556
Kzo9I GATC 2 cut(s) 141, 208
LguI GCTCTTC 1 cut(s) 439
LpnPI CCDG 9 cut(s) 102, 146, 191, 201, 339, 366, 558, 561, 582
Lsp1109I GCAGC 2 cut(s) 181, 295
LweI GCATC 1 cut(s) 240
MaeIII GTNAC 2 cut(s) 271, 406
MalI GATC 2 cut(s) 143, 210
MboI GATC 2 cut(s) 141, 208
MboII GAAGA 5 cut(s) 57, 235, 456, 547, 560
MfeI CAATTG 1 cut(s) 427
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 2 cut(s) 451, 510
MlsI TGGCCA 1 cut(s) 402
MluCI AATT 3 cut(s) 62, 427, 494
MluNI TGGCCA 1 cut(s) 402
MlyI GAGTC 2 cut(s) 73, 375
MnlI CCTC 4 cut(s) 28, 63, 69, 505
Mox20I TGGCCA 1 cut(s) 402
MscI TGGCCA 1 cut(s) 402
MslI CAYNNNNRTG 1 cut(s) 402
Msp20I TGGCCA 1 cut(s) 402
MspA1I CMGCKG 1 cut(s) 286
MspR9I CCNGG 1 cut(s) 354
MunI CAATTG 1 cut(s) 427
MvaI CCWGG 1 cut(s) 354
MwoI GCNNNNNNNGC 4 cut(s) 152, 197, 292, 472
NdeII GATC 2 cut(s) 141, 208
NlaIII CATG 5 cut(s) 109, 401, 407, 490, 556
NlaIV GGNNCC 2 cut(s) 434, 474
NspI RCATGY 1 cut(s) 556
NspV TTCGAA 1 cut(s) 562
PagI TCATGA 1 cut(s) 105
PceI AGGCCT 1 cut(s) 191
PciI ACATGT 1 cut(s) 552
PciSI GCTCTTC 1 cut(s) 439
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PfeI GAWTC 1 cut(s) 572
PflFI GACNNNGTC 1 cut(s) 119
PflMI CCANNNNNTGG 1 cut(s) 260
PfoI TCCNGGA 1 cut(s) 352
PkrI GCNGC 2 cut(s) 171, 285
PleI GAGTC 2 cut(s) 73, 374
PpsI GAGTC 2 cut(s) 73, 374
PscI ACATGT 1 cut(s) 552
Psp124BI GAGCTC 1 cut(s) 451
Psp6I CCWGG 1 cut(s) 352
PspGI CCWGG 1 cut(s) 352
PspN4I GGNNCC 2 cut(s) 434, 474
PsuI RGATCY 1 cut(s) 208
PsyI GACNNNGTC 1 cut(s) 119
RsaI GTAC 2 cut(s) 239, 557
RsaNI GTAC 2 cut(s) 238, 556
RseI CAYNNNNRTG 1 cut(s) 402
SacI GAGCTC 1 cut(s) 451
SapI GCTCTTC 1 cut(s) 439
SatI GCNGC 2 cut(s) 170, 284
Sau3AI GATC 2 cut(s) 141, 208
SchI GAGTC 2 cut(s) 73, 375
ScrFI CCNGG 1 cut(s) 354
SduI GDGCHC 2 cut(s) 451, 510
SetI ASST 6 cut(s) 27, 174, 273, 451, 516, 547
SfaNI GCATC 1 cut(s) 240
SfuI TTCGAA 1 cut(s) 562
SmiMI CAYNNNNRTG 1 cut(s) 402
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 3 cut(s) 62, 427, 494
SseBI AGGCCT 1 cut(s) 191
SsiI CCGC 1 cut(s) 286
SstI GAGCTC 1 cut(s) 451
StuI AGGCCT 1 cut(s) 191
StyD4I CCNGG 1 cut(s) 352
StyI CCWWGG 2 cut(s) 381, 525
TaqI TCGA 2 cut(s) 123, 562
TasI AATT 3 cut(s) 62, 427, 494
TatI WGTACW 1 cut(s) 555
TfiI GAWTC 1 cut(s) 572
TscAI CASTG 2 cut(s) 63, 202
TseI GCWGC 2 cut(s) 169, 283
TspDTI ATGAA 2 cut(s) 236, 351
TspGWI ACGGA 3 cut(s) 125, 360, 496
TspRI CASTG 2 cut(s) 63, 202
Tth111I GACNNNGTC 1 cut(s) 119
Van91I CCANNNNNTGG 1 cut(s) 260
XagI CCTNNNNNAGG 1 cut(s) 573
XceI RCATGY 1 cut(s) 556
XmiI GTMKAC 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.