MD15G1230800.v1.1

WUSCHEL-related homeobox

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002233
Physical Location & Seq
Reverse (-)
18875969 .. 18878798
2830 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1230800.v1.1.491

Sequence Viewer

Length: 834 bp
ATGATGGAGTGGGAGAAGCAGCAACTGAAAGAGCAGCAAGAGATGGAGGGGAACGGGAACGGGAGTGGGAATAATGGGAATGGAGGAGTGATGTATGTGAAGGTGATGACTGATGAGCAGTTGGAGACTCTGAGGAAACAGATTGCAGTTTATGCCACCATCTGTGAGCAGCTTGTTGAGATGCACAAGAACCTCACTTCCCAGCAAGATCTTGCAGGAGTCAGGATGGGAAATCTGTACTGCGATCCGCTGATGACATCCACCGGCCACAAAATTACCGCCAGGCAGCGGTGGACGCCAACACCAGTGCAGCTCCAAATTCTTGAGCGTTTATTCGAGCAAGGGAATGGAACTCCGAGCAAGCAGAAGATCAAGGAGATCACCACTGAGCTGAGCCAGCACGGACAAATTTCCGAAACAAATGTGTACAATTGGTTCCAGAACAGGCGCGCTCGATCAAAAAGGAAGCAGCAAAATGCAGCATGTAACCATGCTGAATCAGAAGTGGAGACGGAAGTTGACTCACCCAAGGACAAGAAAACTAGACCCGAGGAAACGTTTCAGTCCCAGCAGCACTCAGCTCAAAGGACCGAAGATTTGTGCTTCCCGAGCCCGGAGATAAGTTCTGACCTGCATTTCTTGGATCCACATGCCACTACGGTGGATAAAATGTTCCCGTCACACAGCGGTTTAAGAAGTTCTAGGCATTTAAGCGAAATGTCATTCTATGATGAAGTGCTTGCAAACTCAAGGCATGAGCTGATGACCGGAAAGATGGAAGCCGGAAATTATAATCTTTTTCAGGAGGCAGAAGACTACGGCCTGACGAGTTGA

Protein Analysis

278

Amino Acids

31.65

Weight (kDa)

5.62

Isoelectric Point (pI)

62.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 95 - 155 8.4e-17 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 792
Acc36I ACCTGC 1 cut(s) 639
AccII CGCG 1 cut(s) 450
AciI CCGC 4 cut(s) 248, 279, 289, 687
AclI AACGTT 1 cut(s) 557
AclWI GGATC 3 cut(s) 239, 638, 651
AcoI YGGCCR 1 cut(s) 265
AcsI RAATTY 2 cut(s) 318, 408
AcyI GRCGYC 1 cut(s) 296
AfaI GTAC 2 cut(s) 239, 428
AfiI CCNNNNNNNGG 2 cut(s) 288, 613
AjnI CCWGG 1 cut(s) 281
AleI CACNNNNGTG 1 cut(s) 659
AloI GAACNNNNNNTCC 4 cut(s) 182, 214, 656, 688
AluBI AGCT 5 cut(s) 172, 313, 391, 581, 760
AluI AGCT 5 cut(s) 172, 313, 391, 581, 760
Alw26I GTCTC 2 cut(s) 119, 503
AlwI GGATC 3 cut(s) 239, 638, 651
AlwNI CAGNNNCTG 1 cut(s) 25
Ama87I CYCGRG 2 cut(s) 548, 607
AoxI GGCC 2 cut(s) 265, 820
ApeKI GCWGC 8 cut(s) 19, 34, 169, 286, 310, 469, 479, 571
ApoI RAATTY 2 cut(s) 318, 408
ArsI GACNNNNNNTTYG 2 cut(s) 580, 612
Asp700I GAANNNNTTC 1 cut(s) 558
AspLEI GCGC 2 cut(s) 450, 452
AspS9I GGNCC 1 cut(s) 588
AsuC2I CCSGG 1 cut(s) 614
AsuHPI GGTGA 3 cut(s) 115, 373, 516
AvaI CYCGRG 2 cut(s) 548, 607
AvaII GGWCC 1 cut(s) 588
BamHI GGATCC 1 cut(s) 643
BanII GRGCYC 1 cut(s) 614
BbsI GAAGAC 1 cut(s) 819
BbvI GCAGC 8 cut(s) 31, 46, 181, 298, 322, 481, 491, 583
BccI CCATC 4 cut(s) 37, 167, 220, 769
BciT130I CCWGG 1 cut(s) 283
BcnI CCSGG 1 cut(s) 614
BcoDI GTCTC 2 cut(s) 119, 503
BfaI CTAG 2 cut(s) 543, 702
BfuAI ACCTGC 1 cut(s) 639
BglII AGATCT 1 cut(s) 208
BisI GCNGC 8 cut(s) 20, 35, 170, 287, 311, 470, 480, 572
BlpI GCTNAGC 1 cut(s) 392
BlsI GCNGC 8 cut(s) 21, 36, 171, 288, 312, 471, 481, 573
Bme1390I CCNGG 2 cut(s) 283, 614
Bme18I GGWCC 1 cut(s) 588
BmeT110I CYCGRG 2 cut(s) 548, 607
BmgT120I GGNCC 1 cut(s) 588
BmiI GGNNCC 2 cut(s) 437, 645
BmrFI CCNGG 2 cut(s) 283, 614
BmsI GCATC 1 cut(s) 171
BpiI GAAGAC 1 cut(s) 819
Bpu1102I GCTNAGC 1 cut(s) 392
BpuEI CTTGAG 2 cut(s) 344, 733
BpuMI CCSGG 1 cut(s) 614
BsaHI GRCGYC 1 cut(s) 296
BsaJI CCNNGG 2 cut(s) 528, 549
BsaWI WCCGGW 1 cut(s) 767
Bsc4I CCNNNNNNNGG 2 cut(s) 288, 613
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 1 cut(s) 305
BseBI CCWGG 1 cut(s) 283
BseDI CCNNGG 2 cut(s) 528, 549
BseGI GGATG 2 cut(s) 231, 257
BseLI CCNNNNNNNGG 2 cut(s) 288, 613
BseMII CTCAG 4 cut(s) 122, 378, 383, 591
BseNI ACTGG 1 cut(s) 305
BsePI GCGCGC 1 cut(s) 448
BseRI GAGGAG 1 cut(s) 99
BseXI GCAGC 8 cut(s) 31, 46, 181, 298, 322, 481, 491, 583
BseYI CCCAGC 2 cut(s) 201, 567
BsgI GTGCAG 1 cut(s) 329
Bsh1236I CGCG 1 cut(s) 450
BshFI GGCC 2 cut(s) 267, 822
BsiHKCI CYCGRG 2 cut(s) 548, 607
BsiSI CCGG 4 cut(s) 264, 614, 768, 783
BslFI GGGAC 1 cut(s) 550
BslI CCNNNNNNNGG 2 cut(s) 288, 613
BsmAI GTCTC 2 cut(s) 119, 503
BsmBI CGTCTC 1 cut(s) 503
BsmFI GGGAC 1 cut(s) 550
BsnI GGCC 2 cut(s) 267, 822
BsoBI CYCGRG 2 cut(s) 548, 607
Bsp1286I GDGCHC 1 cut(s) 614
Bsp1407I TGTACA 1 cut(s) 426
Bsp143I GATC 6 cut(s) 208, 244, 369, 378, 455, 643
Bsp1720I GCTNAGC 1 cut(s) 392
BspACI CCGC 4 cut(s) 248, 279, 289, 687
BspANI GGCC 2 cut(s) 267, 822
BspCNI CTCAG 4 cut(s) 123, 379, 384, 590
BspFNI CGCG 1 cut(s) 450
BspLI GGNNCC 2 cut(s) 437, 645
BspMI ACCTGC 1 cut(s) 639
BspPI GGATC 3 cut(s) 239, 638, 651
BsrFI RCCGGY 1 cut(s) 263
BsrGI TGTACA 1 cut(s) 426
BsrI ACTGG 1 cut(s) 305
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 2 cut(s) 528, 549
BssHII GCGCGC 1 cut(s) 448
BssMI GATC 6 cut(s) 208, 244, 369, 378, 455, 643
BssNI GRCGYC 1 cut(s) 296
BssT1I CCWWGG 1 cut(s) 528
Bst2UI CCWGG 1 cut(s) 283
Bst4CI ACNGT 1 cut(s) 661
BstACI GRCGYC 1 cut(s) 296
BstAPI GCANNNNNTGC 1 cut(s) 152
BstAUI TGTACA 1 cut(s) 426
BstC8I GCNNGC 4 cut(s) 362, 398, 450, 741
BstDEI CTNAG 4 cut(s) 131, 387, 392, 577
BstF5I GGATG 2 cut(s) 231, 257
BstFNI CGCG 1 cut(s) 450
BstHHI GCGC 2 cut(s) 450, 452
BstKTI GATC 6 cut(s) 211, 247, 372, 381, 458, 646
BstMAI GTCTC 2 cut(s) 119, 503
BstMBI GATC 6 cut(s) 208, 244, 369, 378, 455, 643
BstMWI GCNNNNNNNGC 4 cut(s) 152, 295, 397, 609
BstNI CCWGG 1 cut(s) 283
BstNSI RCATGY 2 cut(s) 486, 653
BstSCI CCNGG 2 cut(s) 281, 612
BstUI CGCG 1 cut(s) 450
BstV1I GCAGC 8 cut(s) 31, 46, 181, 298, 322, 481, 491, 583
BstV2I GAAGAC 1 cut(s) 819
BstX2I RGATCY 2 cut(s) 208, 643
BstXI CCANNNNNNTGG 1 cut(s) 661
BstYI RGATCY 2 cut(s) 208, 643
BsuRI GGCC 2 cut(s) 267, 822
BtsCI GGATG 2 cut(s) 231, 257
BtsIMutI CAGTG 2 cut(s) 312, 384
BveI ACCTGC 1 cut(s) 639
Cac8I GCNNGC 4 cut(s) 362, 398, 450, 741
CaiI CAGNNNCTG 1 cut(s) 25
CfoI GCGC 2 cut(s) 450, 452
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 1 cut(s) 588
CseI GACGC 1 cut(s) 304
Csp6I GTAC 2 cut(s) 238, 427
CviAII CATG 4 cut(s) 483, 491, 650, 755
CviQI GTAC 2 cut(s) 238, 427
DdeI CTNAG 4 cut(s) 131, 387, 392, 577
DpnI GATC 6 cut(s) 210, 246, 371, 380, 457, 645
DpnII GATC 6 cut(s) 208, 244, 369, 378, 455, 643
EaeI YGGCCR 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 528
Eco24I GRGCYC 1 cut(s) 614
Eco47I GGWCC 1 cut(s) 588
Eco88I CYCGRG 2 cut(s) 548, 607
EcoRII CCWGG 1 cut(s) 281
EcoT14I CCWWGG 1 cut(s) 528
EcoT38I GRGCYC 1 cut(s) 614
ErhI CCWWGG 1 cut(s) 528
Esp3I CGTCTC 1 cut(s) 503
FaeI CATG 4 cut(s) 486, 494, 653, 758
FaiI YATR 8 cut(s) 96, 153, 484, 492, 651, 729, 756, 792
FaqI GGGAC 1 cut(s) 550
FatI CATG 4 cut(s) 482, 490, 649, 754
Fnu4HI GCNGC 8 cut(s) 20, 35, 170, 287, 311, 470, 480, 572
FokI GGATG 2 cut(s) 238, 244
FriOI GRGCYC 1 cut(s) 614
Fsp4HI GCNGC 8 cut(s) 20, 35, 170, 287, 311, 470, 480, 572
FspBI CTAG 2 cut(s) 543, 702
GlaI GCGC 2 cut(s) 449, 451
GluI GCNGC 8 cut(s) 20, 35, 170, 287, 311, 470, 480, 572
GsaI CCCAGC 2 cut(s) 205, 571
HaeIII GGCC 2 cut(s) 267, 822
HapII CCGG 4 cut(s) 264, 614, 768, 783
HgaI GACGC 1 cut(s) 304
HhaI GCGC 2 cut(s) 450, 452
Hin1I GRCGYC 1 cut(s) 296
Hin1II CATG 4 cut(s) 486, 494, 653, 758
Hin6I GCGC 2 cut(s) 448, 450
HinP1I GCGC 2 cut(s) 448, 450
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HinfI GANTC 4 cut(s) 127, 219, 497, 521
HpaII CCGG 4 cut(s) 264, 614, 768, 783
HphI GGTGA 3 cut(s) 115, 373, 516
Hpy166II GTNNAC 3 cut(s) 294, 427, 520
Hpy188I TCNGA 5 cut(s) 132, 357, 415, 502, 628
Hpy188III TCNNGA 5 cut(s) 223, 323, 439, 607, 803
Hpy8I GTNNAC 3 cut(s) 294, 427, 520
HpyAV CCTTC 1 cut(s) 94
HpyCH4III ACNGT 1 cut(s) 661
HpyCH4IV ACGT 1 cut(s) 557
HpyCH4V TGCA 7 cut(s) 146, 184, 215, 310, 479, 634, 743
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 295, 397, 609
HpyF3I CTNAG 4 cut(s) 131, 387, 392, 577
HpySE526I ACGT 1 cut(s) 557
Hsp92I GRCGYC 1 cut(s) 296
Hsp92II CATG 4 cut(s) 486, 494, 653, 758
HspAI GCGC 2 cut(s) 448, 450
Kzo9I GATC 6 cut(s) 208, 244, 369, 378, 455, 643
LmnI GCTCC 1 cut(s) 318
Lsp1109I GCAGC 8 cut(s) 31, 46, 181, 298, 322, 481, 491, 583
LweI GCATC 1 cut(s) 171
MaeI CTAG 2 cut(s) 543, 702
MaeII ACGT 1 cut(s) 557
MaeIII GTNAC 2 cut(s) 485, 678
MalI GATC 6 cut(s) 210, 246, 371, 380, 457, 645
MboI GATC 6 cut(s) 208, 244, 369, 378, 455, 643
MboII GAAGA 3 cut(s) 379, 605, 824
MfeI CAATTG 1 cut(s) 430
MflI RGATCY 2 cut(s) 208, 643
MhlI GDGCHC 1 cut(s) 614
MluCI AATT 5 cut(s) 273, 318, 408, 430, 787
MlyI GAGTC 3 cut(s) 121, 228, 515
MmeI TCCRAC 1 cut(s) 102
MnlI CCTC 6 cut(s) 40, 77, 126, 203, 544, 799
MroXI GAANNNNTTC 1 cut(s) 558
MseI TTAA 2 cut(s) 692, 710
MslI CAYNNNNRTG 1 cut(s) 659
MspA1I CMGCKG 3 cut(s) 250, 289, 687
MspI CCGG 4 cut(s) 264, 614, 768, 783
MspR9I CCNGG 2 cut(s) 283, 614
MunI CAATTG 1 cut(s) 430
MvaI CCWGG 1 cut(s) 283
MvnI CGCG 1 cut(s) 450
MwoI GCNNNNNNNGC 4 cut(s) 152, 295, 397, 609
NciI CCSGG 1 cut(s) 614
NdeII GATC 6 cut(s) 208, 244, 369, 378, 455, 643
NlaIII CATG 4 cut(s) 486, 494, 653, 758
NlaIV GGNNCC 2 cut(s) 437, 645
NmuCI GTSAC 1 cut(s) 678
NspI RCATGY 2 cut(s) 486, 653
OliI CACNNNNGTG 1 cut(s) 659
PauI GCGCGC 1 cut(s) 448
PdmI GAANNNNTTC 1 cut(s) 558
PfeI GAWTC 1 cut(s) 497
PkrI GCNGC 8 cut(s) 21, 36, 171, 288, 312, 471, 481, 573
PleI GAGTC 3 cut(s) 121, 227, 515
PpsI GAGTC 3 cut(s) 121, 227, 515
PsiI TTATAA 1 cut(s) 792
Psp1406I AACGTT 1 cut(s) 557
Psp6I CCWGG 1 cut(s) 281
PspFI CCCAGC 2 cut(s) 201, 567
PspGI CCWGG 1 cut(s) 281
PspN4I GGNNCC 2 cut(s) 437, 645
PspPI GGNCC 1 cut(s) 588
PsrI GAACNNNNNNTAC 2 cut(s) 419, 451
PstNI CAGNNNCTG 1 cut(s) 25
PsuI RGATCY 2 cut(s) 208, 643
PteI GCGCGC 1 cut(s) 448
RsaI GTAC 2 cut(s) 239, 428
RsaNI GTAC 2 cut(s) 238, 427
RseI CAYNNNNRTG 1 cut(s) 659
SaqAI TTAA 2 cut(s) 692, 710
SatI GCNGC 8 cut(s) 20, 35, 170, 287, 311, 470, 480, 572
Sau3AI GATC 6 cut(s) 208, 244, 369, 378, 455, 643
Sau96I GGNCC 1 cut(s) 588
SchI GAGTC 3 cut(s) 121, 228, 515
ScrFI CCNGG 2 cut(s) 283, 614
SduI GDGCHC 1 cut(s) 614
SetI ASST 9 cut(s) 105, 174, 195, 315, 393, 560, 583, 633, 762
SfaNI GCATC 1 cut(s) 171
SinI GGWCC 1 cut(s) 588
SmiMI CAYNNNNRTG 1 cut(s) 659
SmlI CTYRAG 2 cut(s) 323, 748
SmoI CTYRAG 2 cut(s) 323, 748
Sse9I AATT 5 cut(s) 273, 318, 408, 430, 787
SsiI CCGC 4 cut(s) 248, 279, 289, 687
SspMI CTAG 2 cut(s) 543, 702
StyD4I CCNGG 2 cut(s) 281, 612
StyI CCWWGG 1 cut(s) 528
TaaI ACNGT 1 cut(s) 661
TaiI ACGT 1 cut(s) 560
TaqI TCGA 2 cut(s) 336, 454
TaqII GACCGA 1 cut(s) 605
TasI AATT 5 cut(s) 273, 318, 408, 430, 787
TatI WGTACW 2 cut(s) 237, 426
TfiI GAWTC 1 cut(s) 497
Tru1I TTAA 2 cut(s) 692, 710
Tru9I TTAA 2 cut(s) 692, 710
TscAI CASTG 2 cut(s) 312, 391
TseFI GTSAC 1 cut(s) 678
TseI GCWGC 8 cut(s) 19, 34, 169, 286, 310, 469, 479, 571
Tsp45I GTSAC 1 cut(s) 678
TspDTI ATGAA 1 cut(s) 747
TspGWI ACGGA 2 cut(s) 417, 527
TspRI CASTG 2 cut(s) 312, 391
VpaK11BI GGWCC 1 cut(s) 588
XapI RAATTY 2 cut(s) 318, 408
XceI RCATGY 2 cut(s) 486, 653
XmnI GAANNNNTTC 1 cut(s) 558
XspI CTAG 2 cut(s) 543, 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.