FvH4_5g25620

Casein Kinase 2 substrate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002014
Physical Location & Seq
Forward (+)
17125110 .. 17130801
5692 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g25620.t2

Sequence Viewer

Length: 600 bp
ATGGAGGCGATGGTGAAGAAATACCAGCAGAGGTTCAGGAAGGTTAGGGACGAGATGGAGACCTGGGCCGAGCTTCAATCTCTCTTGACTTCACAGTTCAGAAATGCCTCCTCCATCATCCAGAGGTTGCAGGTGCTTCAAGATTCAAACAACTATGGTGAGGGCTTGCGTGGAGTTGCCCATCTTCAAGAAGCCGTGCTGCTGAAGCAGATGGAGTCTTTGCAAAAGCTCTTGCTTTCTATGAACAATACCTTGGAACAGTTTCATGGTATTGTGTTGTCTCTTGGGAGGATTCATCGTGACGGTAGGCAGATGATAAAAGGTGGGTCTAGTCAGCTGAGTTTCAAACAACTGCAGCAGCGAGTTGGTGTGAAACCGAGTCTTGCAGATTGCGTAGATGGGCTCATGCTTCTTCAGGACATGCATTGCTCTGAGTACCTTTTAAAGTCATCACTGGTTTCAGCACTATCAGAACTTACATCGAAACCCAGTGCCAGCGATCTGGGTTCCCTCCAGCAAATCTTGATTGATCAGCCCAACATCCCCAAGGAGGAAGTTCAGTTCATCTTTGATATCATATTTGCTGAAGAAATTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CK2S

Protein Analysis

200

Amino Acids

22.55

Weight (kDa)

6.1

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CA109-like PF15011 7 - 169 3e-51 Ribosome biogenesis protein C1orf109-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 121
Acc36I ACCTGC 1 cut(s) 121
AcsI RAATTY 1 cut(s) 591
AcuI CTGAAG 2 cut(s) 224, 398
AfaI GTAC 1 cut(s) 437
AfiI CCNNNNNNNGG 1 cut(s) 550
AgsI TTSAA 5 cut(s) 77, 140, 147, 188, 346
AjnI CCWGG 1 cut(s) 62
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AloI GAACNNNNNNTCC 2 cut(s) 545, 577
AluBI AGCT 3 cut(s) 73, 229, 337
AluI AGCT 3 cut(s) 73, 229, 337
Alw26I GTCTC 2 cut(s) 53, 285
AoxI GGCC 1 cut(s) 66
ApeKI GCWGC 3 cut(s) 199, 355, 358
ApoI RAATTY 1 cut(s) 591
Asp700I GAANNNNTTC 1 cut(s) 261
AspS9I GGNCC 1 cut(s) 66
AsuHPI GGTGA 2 cut(s) 25, 170
BanII GRGCYC 1 cut(s) 405
BbvI GCAGC 3 cut(s) 186, 367, 370
BccI CCATC 6 cut(s) 4, 49, 122, 189, 205, 392
BceAI ACGGC 1 cut(s) 179
BciT130I CCWGG 1 cut(s) 64
BclI TGATCA 1 cut(s) 529
BcoDI GTCTC 2 cut(s) 53, 285
BfaI CTAG 1 cut(s) 330
BfmI CTRYAG 1 cut(s) 353
BfuAI ACCTGC 1 cut(s) 121
BisI GCNGC 3 cut(s) 200, 356, 359
BlsI GCNGC 3 cut(s) 201, 357, 360
Bme1390I CCNGG 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 66
BmiI GGNNCC 1 cut(s) 508
BmrFI CCNGG 1 cut(s) 64
BmrI ACTGGG 1 cut(s) 483
BmuI ACTGGG 1 cut(s) 483
BpmI CTGGAG 1 cut(s) 497
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 3 cut(s) 63, 252, 546
Bsc4I CCNNNNNNNGG 1 cut(s) 550
Bse1I ACTGG 2 cut(s) 459, 489
Bse3DI GCAATG 1 cut(s) 424
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 3 cut(s) 63, 252, 546
BseGI GGATG 2 cut(s) 117, 540
BseLI CCNNNNNNNGG 1 cut(s) 550
BseMI GCAATG 1 cut(s) 424
BseMII CTCAG 2 cut(s) 329, 423
BseNI ACTGG 2 cut(s) 459, 489
BseRI GAGGAG 1 cut(s) 100
BseXI GCAGC 3 cut(s) 186, 367, 370
BshFI GGCC 1 cut(s) 68
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 1 cut(s) 550
BsmAI GTCTC 2 cut(s) 53, 285
BsmFI GGGAC 1 cut(s) 62
BsnI GGCC 1 cut(s) 68
Bso31I GGTCTC 1 cut(s) 53
Bsp1286I GDGCHC 1 cut(s) 405
Bsp143I GATC 2 cut(s) 499, 529
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 2 cut(s) 330, 424
BspLI GGNNCC 1 cut(s) 508
BspMAI CTGCAG 1 cut(s) 357
BspMI ACCTGC 1 cut(s) 121
BspTNI GGTCTC 1 cut(s) 53
BsrDI GCAATG 1 cut(s) 424
BsrI ACTGG 2 cut(s) 459, 489
BssECI CCNNGG 3 cut(s) 63, 252, 546
BssMI GATC 2 cut(s) 499, 529
BssT1I CCWWGG 2 cut(s) 252, 546
Bst2UI CCWGG 1 cut(s) 64
Bst4CI ACNGT 3 cut(s) 96, 261, 305
BstC8I GCNNGC 2 cut(s) 167, 496
BstDEI CTNAG 2 cut(s) 338, 432
BstF5I GGATG 2 cut(s) 117, 540
BstKTI GATC 2 cut(s) 502, 532
BstMAI GTCTC 2 cut(s) 53, 285
BstMBI GATC 2 cut(s) 499, 529
BstMWI GCNNNNNNNGC 1 cut(s) 205
BstNI CCWGG 1 cut(s) 64
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 1 cut(s) 62
BstSFI CTRYAG 1 cut(s) 353
BstV1I GCAGC 3 cut(s) 186, 367, 370
BstXI CCANNNNNNTGG 1 cut(s) 502
BsuRI GGCC 1 cut(s) 68
BtgZI GCGATG 1 cut(s) 23
BtsCI GGATG 2 cut(s) 117, 540
BtsIMutI CAGTG 2 cut(s) 452, 496
BveI ACCTGC 1 cut(s) 121
Cac8I GCNNGC 2 cut(s) 167, 496
Cfr13I GGNCC 1 cut(s) 66
Csp6I GTAC 1 cut(s) 436
CviAII CATG 3 cut(s) 266, 406, 421
CviJI RGCY 8 cut(s) 68, 73, 165, 194, 229, 337, 403, 535
CviKI_1 RGCY 8 cut(s) 68, 73, 165, 194, 229, 337, 403, 535
CviQI GTAC 1 cut(s) 436
DdeI CTNAG 2 cut(s) 338, 432
DpnI GATC 2 cut(s) 501, 531
DpnII GATC 2 cut(s) 499, 529
DraI TTTAAA 1 cut(s) 444
Eco130I CCWWGG 2 cut(s) 252, 546
Eco24I GRGCYC 1 cut(s) 405
Eco31I GGTCTC 1 cut(s) 53
Eco32I GATATC 1 cut(s) 574
Eco57I CTGAAG 2 cut(s) 224, 398
EcoRII CCWGG 1 cut(s) 62
EcoRV GATATC 1 cut(s) 574
EcoT14I CCWWGG 2 cut(s) 252, 546
EcoT22I ATGCAT 1 cut(s) 426
EcoT38I GRGCYC 1 cut(s) 405
ErhI CCWWGG 2 cut(s) 252, 546
FaeI CATG 3 cut(s) 269, 409, 424
FaiI YATR 6 cut(s) 156, 242, 267, 407, 422, 578
FaqI GGGAC 1 cut(s) 62
FatI CATG 3 cut(s) 265, 405, 420
FbaI TGATCA 1 cut(s) 529
Fnu4HI GCNGC 3 cut(s) 200, 356, 359
FokI GGATG 2 cut(s) 104, 527
FriOI GRGCYC 1 cut(s) 405
Fsp4HI GCNGC 3 cut(s) 200, 356, 359
FspBI CTAG 1 cut(s) 330
GluI GCNGC 3 cut(s) 200, 356, 359
GsuI CTGGAG 1 cut(s) 497
HaeIII GGCC 1 cut(s) 68
Hin1II CATG 3 cut(s) 269, 409, 424
HinfI GANTC 4 cut(s) 143, 215, 292, 379
HphI GGTGA 2 cut(s) 25, 170
Hpy188I TCNGA 3 cut(s) 101, 433, 472
Hpy188III TCNNGA 8 cut(s) 37, 85, 121, 140, 188, 299, 416, 523
HpyAV CCTTC 1 cut(s) 34
HpyCH4III ACNGT 3 cut(s) 96, 261, 305
HpyCH4V TGCA 5 cut(s) 130, 223, 355, 386, 424
HpyF10VI GCNNNNNNNGC 1 cut(s) 205
HpyF3I CTNAG 2 cut(s) 338, 432
Hsp92II CATG 3 cut(s) 269, 409, 424
Ksp22I TGATCA 1 cut(s) 529
Kzo9I GATC 2 cut(s) 499, 529
Lsp1109I GCAGC 3 cut(s) 186, 367, 370
MaeI CTAG 1 cut(s) 330
MaeIII GTNAC 1 cut(s) 299
MalI GATC 2 cut(s) 501, 531
MboI GATC 2 cut(s) 499, 529
MboII GAAGA 4 cut(s) 28, 176, 404, 599
MhlI GDGCHC 1 cut(s) 405
MluCI AATT 1 cut(s) 591
MlyI GAGTC 2 cut(s) 224, 388
MnlI CCTC 8 cut(s) 24, 117, 118, 121, 154, 282, 521, 544
Mph1103I ATGCAT 1 cut(s) 426
MroXI GAANNNNTTC 1 cut(s) 261
MseI TTAA 1 cut(s) 443
MspA1I CMGCKG 1 cut(s) 337
MspR9I CCNGG 1 cut(s) 64
MvaI CCWGG 1 cut(s) 64
MwoI GCNNNNNNNGC 1 cut(s) 205
NdeII GATC 2 cut(s) 499, 529
NlaIII CATG 3 cut(s) 269, 409, 424
NlaIV GGNNCC 1 cut(s) 508
NmeAIII GCCGAG 1 cut(s) 94
NmuCI GTSAC 1 cut(s) 299
NsiI ATGCAT 1 cut(s) 426
NspI RCATGY 1 cut(s) 424
PaqCI CACCTGC 1 cut(s) 121
PdmI GAANNNNTTC 1 cut(s) 261
PfeI GAWTC 2 cut(s) 143, 292
PkrI GCNGC 3 cut(s) 201, 357, 360
PleI GAGTC 2 cut(s) 223, 387
PpsI GAGTC 2 cut(s) 223, 387
Psp6I CCWGG 1 cut(s) 62
PspGI CCWGG 1 cut(s) 62
PspN4I GGNNCC 1 cut(s) 508
PspPI GGNCC 1 cut(s) 66
PstI CTGCAG 1 cut(s) 357
PvuII CAGCTG 1 cut(s) 337
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
SaqAI TTAA 1 cut(s) 443
SatI GCNGC 3 cut(s) 200, 356, 359
Sau3AI GATC 2 cut(s) 499, 529
Sau96I GGNCC 1 cut(s) 66
SchI GAGTC 2 cut(s) 224, 388
ScrFI CCNGG 1 cut(s) 64
SduI GDGCHC 1 cut(s) 405
SfcI CTRYAG 1 cut(s) 353
Sse9I AATT 1 cut(s) 591
SspMI CTAG 1 cut(s) 330
StyD4I CCNGG 1 cut(s) 62
StyI CCWWGG 2 cut(s) 252, 546
TaaI ACNGT 3 cut(s) 96, 261, 305
TaqI TCGA 1 cut(s) 482
TasI AATT 1 cut(s) 591
TfiI GAWTC 2 cut(s) 143, 292
Tru1I TTAA 1 cut(s) 443
Tru9I TTAA 1 cut(s) 443
TscAI CASTG 2 cut(s) 459, 496
TseFI GTSAC 1 cut(s) 299
TseI GCWGC 3 cut(s) 199, 355, 358
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 4 cut(s) 254, 257, 284, 553
TspRI CASTG 2 cut(s) 459, 496
XapI RAATTY 1 cut(s) 591
XceI RCATGY 1 cut(s) 424
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 1 cut(s) 330
Zsp2I ATGCAT 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.