pycom15g38810

Casein Kinase 2 substrate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002014
Physical Location & Seq
Forward (+)
38555614 .. 38558148
2535 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g38810.7

Sequence Viewer

Length: 630 bp
ATGGGACCTATAGCTTCACAAGGGGTAGGGATGGAGGCGATGGTGAAGAGGTACCAGCAGAGGTTCAGGAAGGTTAAGGATGAGATGGATAGCTGGGCCCTGCTTCAGTCTCGCTTGATTTCGCAGTTCAGGAACGCCTCCTCCATCATTCAGAGGTTGCAGGTGCTTCAAGATTCCAAGAACTACGGCCGTTTGAAAGATGTCGTTGGTATCGAAGAGGCGCTACTGGCAAAGCAGATGGAATCATTGCAAAACATCTTCCTTTCCATGAAGAAAACCATGGAAGAGTTTCATGGTATTGTACTGTCTCTTGGGAAGATCCATCGTGATGGTAGGCAGATGGTAAAAGGCGGTGCTTCTGGTCAGCTGAGTGTTAAACAACTGCAGCAACGAATTGGTGTAAAGCCCAGTCTTGCAGATTGCTTAGATGGGCTTATGCTTCTCCAGGACATGCATTGCTCTGAGTACCTTTTGAAGTCATCATTGGTTTCAGCACTGTCAGAACTGACCTTGAAACCCAGTGCCAGTGATTTGGGAGCACTCCAGCAGCTCTTGGTTGATCAGCCTAACATCCCCAAGGAGGAAGTTCAATTCATCTTTGATATCATATTTGCAGAAGAAATCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CK2S

Protein Analysis

210

Amino Acids

23.44

Weight (kDa)

7.7

Isoelectric Point (pI)

52.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 151
Acc36I ACCTGC 1 cut(s) 151
Acc65I GGTACC 1 cut(s) 51
AccB1I GGYRCC 1 cut(s) 51
AciI CCGC 1 cut(s) 351
AclWI GGATC 1 cut(s) 313
AcoI YGGCCR 1 cut(s) 187
AcuI CTGAAG 1 cut(s) 89
AfaI GTAC 3 cut(s) 53, 303, 467
AfiI CCNNNNNNNGG 1 cut(s) 580
AgsI TTSAA 5 cut(s) 170, 196, 475, 514, 590
AjnI CCWGG 1 cut(s) 444
AjuI GAANNNNNNNTTGG 2 cut(s) 467, 499
AloI GAACNNNNNNTCC 2 cut(s) 125, 157
AluBI AGCT 4 cut(s) 14, 93, 367, 550
AluI AGCT 4 cut(s) 14, 93, 367, 550
Alw21I GWGCWC 1 cut(s) 541
Alw26I GTCTC 2 cut(s) 114, 312
AlwI GGATC 1 cut(s) 313
AoxI GGCC 2 cut(s) 96, 187
ApaI GGGCCC 1 cut(s) 100
ApeKI GCWGC 2 cut(s) 385, 547
Asp700I GAANNNNTTC 1 cut(s) 288
Asp718I GGTACC 1 cut(s) 51
AspLEI GCGC 1 cut(s) 223
AspS9I GGNCC 3 cut(s) 5, 96, 97
AsuHPI GGTGA 1 cut(s) 55
AvaII GGWCC 1 cut(s) 5
BaeGI GKGCMC 1 cut(s) 100
BanI GGYRCC 1 cut(s) 51
BanII GRGCYC 1 cut(s) 100
BarI GAAGNNNNNNTAC 2 cut(s) 207, 239
Bbv12I GWGCWC 1 cut(s) 541
BbvI GCAGC 2 cut(s) 397, 559
BccI CCATC 9 cut(s) 25, 34, 79, 152, 232, 323, 330, 334, 422
BceAI ACGGC 2 cut(s) 174, 202
BciT130I CCWGG 1 cut(s) 446
BclI TGATCA 1 cut(s) 559
BcoDI GTCTC 2 cut(s) 114, 312
BfmI CTRYAG 2 cut(s) 9, 383
BfoI RGCGCY 1 cut(s) 224
BfuAI ACCTGC 1 cut(s) 151
BisI GCNGC 2 cut(s) 386, 548
BlsI GCNGC 2 cut(s) 387, 549
Bme1390I CCNGG 1 cut(s) 446
Bme18I GGWCC 1 cut(s) 5
BmgT120I GGNCC 3 cut(s) 5, 96, 97
BmiI GGNNCC 3 cut(s) 6, 53, 98
BmrFI CCNGG 1 cut(s) 446
BmrI ACTGGG 2 cut(s) 402, 513
BmuI ACTGGG 2 cut(s) 402, 513
BpmI CTGGAG 2 cut(s) 428, 527
BsaJI CCNNGG 2 cut(s) 279, 576
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 580
Bse1I ACTGG 4 cut(s) 231, 408, 519, 525
Bse3DI GCAATG 2 cut(s) 245, 454
BseBI CCWGG 1 cut(s) 446
BseDI CCNNGG 2 cut(s) 279, 576
BseGI GGATG 3 cut(s) 36, 85, 570
BseLI CCNNNNNNNGG 1 cut(s) 580
BseMI GCAATG 2 cut(s) 245, 454
BseMII CTCAG 2 cut(s) 359, 453
BseNI ACTGG 4 cut(s) 231, 408, 519, 525
BseRI GAGGAG 1 cut(s) 130
BseSI GKGCMC 1 cut(s) 100
BseX3I CGGCCG 1 cut(s) 187
BseXI GCAGC 2 cut(s) 397, 559
BseYI CCCAGC 1 cut(s) 93
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 2 cut(s) 98, 189
BshNI GGYRCC 1 cut(s) 51
BsiEI CGRYCG 1 cut(s) 190
BsiHKAI GWGCWC 1 cut(s) 541
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 1 cut(s) 580
BsmAI GTCTC 2 cut(s) 114, 312
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 2 cut(s) 98, 189
Bsp120I GGGCCC 1 cut(s) 96
Bsp1286I GDGCHC 2 cut(s) 100, 541
Bsp143I GATC 2 cut(s) 318, 559
Bsp19I CCATGG 1 cut(s) 279
BspACI CCGC 1 cut(s) 351
BspANI GGCC 2 cut(s) 98, 189
BspCNI CTCAG 2 cut(s) 360, 454
BspLI GGNNCC 3 cut(s) 6, 53, 98
BspMAI CTGCAG 1 cut(s) 387
BspMI ACCTGC 1 cut(s) 151
BspPI GGATC 1 cut(s) 313
BspT107I GGYRCC 1 cut(s) 51
BsrDI GCAATG 2 cut(s) 245, 454
BsrI ACTGG 4 cut(s) 231, 408, 519, 525
BssECI CCNNGG 2 cut(s) 279, 576
BssMI GATC 2 cut(s) 318, 559
BssT1I CCWWGG 2 cut(s) 279, 576
Bst2UI CCWGG 1 cut(s) 446
Bst4CI ACNGT 2 cut(s) 306, 498
Bst6I CTCTTC 3 cut(s) 41, 210, 279
BstDEI CTNAG 3 cut(s) 368, 424, 462
BstDSI CCRYGG 1 cut(s) 279
BstF5I GGATG 3 cut(s) 36, 85, 570
BstH2I RGCGCY 1 cut(s) 224
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 2 cut(s) 321, 562
BstMAI GTCTC 2 cut(s) 114, 312
BstMBI GATC 2 cut(s) 318, 559
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstNI CCWGG 1 cut(s) 446
BstNSI RCATGY 1 cut(s) 454
BstSCI CCNGG 1 cut(s) 444
BstSFI CTRYAG 2 cut(s) 9, 383
BstSLI GKGCMC 1 cut(s) 100
BstV1I GCAGC 2 cut(s) 397, 559
BstX2I RGATCY 1 cut(s) 318
BstXI CCANNNNNNTGG 2 cut(s) 329, 532
BstYI RGATCY 1 cut(s) 318
BstZI CGGCCG 1 cut(s) 187
BsuRI GGCC 2 cut(s) 98, 189
BtgI CCRYGG 1 cut(s) 279
BtgZI GCGATG 1 cut(s) 53
BtsCI GGATG 3 cut(s) 36, 85, 570
BtsIMutI CAGTG 3 cut(s) 494, 526, 532
BveI ACCTGC 1 cut(s) 151
CfoI GCGC 1 cut(s) 223
Cfr13I GGNCC 3 cut(s) 5, 96, 97
Csp6I GTAC 3 cut(s) 52, 302, 466
CviAII CATG 4 cut(s) 268, 280, 293, 451
CviJI RGCY 9 cut(s) 14, 93, 98, 189, 367, 406, 433, 550, 565
CviKI_1 RGCY 9 cut(s) 14, 93, 98, 189, 367, 406, 433, 550, 565
CviQI GTAC 3 cut(s) 52, 302, 466
DdeI CTNAG 3 cut(s) 368, 424, 462
DpnI GATC 2 cut(s) 320, 561
DpnII GATC 2 cut(s) 318, 559
EaeI YGGCCR 1 cut(s) 187
EagI CGGCCG 1 cut(s) 187
Eam1104I CTCTTC 3 cut(s) 41, 210, 279
EarI CTCTTC 3 cut(s) 41, 210, 279
EclXI CGGCCG 1 cut(s) 187
Eco130I CCWWGG 2 cut(s) 279, 576
Eco24I GRGCYC 1 cut(s) 100
Eco32I GATATC 1 cut(s) 604
Eco47I GGWCC 1 cut(s) 5
Eco52I CGGCCG 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 89
EcoO109I RGGNCCY 2 cut(s) 5, 97
EcoRII CCWGG 1 cut(s) 444
EcoRV GATATC 1 cut(s) 604
EcoT14I CCWWGG 2 cut(s) 279, 576
EcoT22I ATGCAT 1 cut(s) 456
EcoT38I GRGCYC 1 cut(s) 100
ErhI CCWWGG 2 cut(s) 279, 576
FaeI CATG 4 cut(s) 271, 283, 296, 454
FaiI YATR 7 cut(s) 11, 269, 281, 294, 437, 452, 608
FaqI GGGAC 1 cut(s) 18
FatI CATG 4 cut(s) 267, 279, 292, 450
FbaI TGATCA 1 cut(s) 559
Fnu4HI GCNGC 2 cut(s) 386, 548
FokI GGATG 3 cut(s) 43, 92, 557
FriOI GRGCYC 1 cut(s) 100
Fsp4HI GCNGC 2 cut(s) 386, 548
GlaI GCGC 1 cut(s) 222
GluI GCNGC 2 cut(s) 386, 548
GsaI CCCAGC 1 cut(s) 97
GsuI CTGGAG 2 cut(s) 428, 527
HaeII RGCGCY 1 cut(s) 224
HaeIII GGCC 2 cut(s) 98, 189
HhaI GCGC 1 cut(s) 223
Hin1II CATG 4 cut(s) 271, 283, 296, 454
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HinfI GANTC 2 cut(s) 173, 242
HphI GGTGA 1 cut(s) 55
Hpy188I TCNGA 3 cut(s) 153, 463, 502
Hpy188III TCNNGA 4 cut(s) 67, 130, 170, 326
HpyAV CCTTC 1 cut(s) 64
HpyCH4III ACNGT 2 cut(s) 306, 498
HpyCH4V TGCA 6 cut(s) 160, 250, 385, 416, 454, 614
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpyF3I CTNAG 3 cut(s) 368, 424, 462
Hsp92II CATG 4 cut(s) 271, 283, 296, 454
HspAI GCGC 1 cut(s) 221
KpnI GGTACC 1 cut(s) 55
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 2 cut(s) 318, 559
LmnI GCTCC 1 cut(s) 536
Lsp1109I GCAGC 2 cut(s) 397, 559
MalI GATC 2 cut(s) 320, 561
MboI GATC 2 cut(s) 318, 559
MboII GAAGA 7 cut(s) 58, 227, 250, 283, 296, 328, 629
MflI RGATCY 1 cut(s) 318
MhlI GDGCHC 2 cut(s) 100, 541
MluCI AATT 2 cut(s) 393, 590
MnlI CCTC 8 cut(s) 28, 42, 54, 147, 148, 151, 211, 574
Mph1103I ATGCAT 1 cut(s) 456
MroXI GAANNNNTTC 1 cut(s) 288
MseI TTAA 2 cut(s) 75, 375
MslI CAYNNNNRTG 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 367
MspR9I CCNGG 1 cut(s) 446
MvaI CCWGG 1 cut(s) 446
MwoI GCNNNNNNNGC 1 cut(s) 227
NcoI CCATGG 1 cut(s) 279
NdeII GATC 2 cut(s) 318, 559
NlaIII CATG 4 cut(s) 271, 283, 296, 454
NlaIV GGNNCC 3 cut(s) 6, 53, 98
NsiI ATGCAT 1 cut(s) 456
NspI RCATGY 1 cut(s) 454
PaqCI CACCTGC 1 cut(s) 151
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 288
PfeI GAWTC 2 cut(s) 173, 242
PfoI TCCNGGA 1 cut(s) 444
PkrI GCNGC 2 cut(s) 387, 549
PpuMI RGGWCCY 1 cut(s) 5
Psp5II RGGWCCY 1 cut(s) 5
Psp6I CCWGG 1 cut(s) 444
PspFI CCCAGC 1 cut(s) 93
PspGI CCWGG 1 cut(s) 444
PspN4I GGNNCC 3 cut(s) 6, 53, 98
PspOMI GGGCCC 1 cut(s) 96
PspPI GGNCC 3 cut(s) 5, 96, 97
PspPPI RGGWCCY 1 cut(s) 5
PstI CTGCAG 1 cut(s) 387
PsuI RGATCY 1 cut(s) 318
PvuII CAGCTG 1 cut(s) 367
RsaI GTAC 3 cut(s) 53, 303, 467
RsaNI GTAC 3 cut(s) 52, 302, 466
RseI CAYNNNNRTG 1 cut(s) 327
SaqAI TTAA 2 cut(s) 75, 375
SatI GCNGC 2 cut(s) 386, 548
Sau3AI GATC 2 cut(s) 318, 559
Sau96I GGNCC 3 cut(s) 5, 96, 97
ScrFI CCNGG 1 cut(s) 446
SduI GDGCHC 2 cut(s) 100, 541
SfcI CTRYAG 2 cut(s) 9, 383
SinI GGWCC 1 cut(s) 5
SmiMI CAYNNNNRTG 1 cut(s) 327
Sse9I AATT 2 cut(s) 393, 590
SsiI CCGC 1 cut(s) 351
StyD4I CCNGG 1 cut(s) 444
StyI CCWWGG 2 cut(s) 279, 576
TaaI ACNGT 2 cut(s) 306, 498
TaqI TCGA 1 cut(s) 213
TasI AATT 2 cut(s) 393, 590
TatI WGTACW 1 cut(s) 301
TfiI GAWTC 2 cut(s) 173, 242
Tru1I TTAA 2 cut(s) 75, 375
Tru9I TTAA 2 cut(s) 75, 375
TscAI CASTG 3 cut(s) 501, 526, 532
TseI GCWGC 2 cut(s) 385, 547
TspDTI ATGAA 3 cut(s) 281, 284, 583
TspRI CASTG 3 cut(s) 501, 526, 532
VpaK11BI GGWCC 1 cut(s) 5
XceI RCATGY 1 cut(s) 454
XmnI GAANNNNTTC 1 cut(s) 288
Zsp2I ATGCAT 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.