pycom08g21270

Casein Kinase 2 substrate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002014
Physical Location & Seq
Forward (+)
20795856 .. 20798014
2159 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g21270.2

Sequence Viewer

Length: 600 bp
ATGGAGGCCATGGTGAAGAAGTATCAGCAGAGGTTCAGGAAGGTGAGGGATGAGATGGACAGCTGGGCCGTGCTTCAGTCTTGCTTGATTTCGCAGTTCAGGAACGCCTCCTCCATCATCCAGAGGTTGCAGGTGCTTCAAGATTCCAAGAATTACGGCTGTTTGAAAGATGTCATCGGTATTGAAGAGGCACTACTGGCAAAGCAGATGGACTCGTTGCAAAACATCTTGCTCTCCATGAAGAAAACCATGGAAGAGTTTCATGTTATTGTATTGTCTCTTGGGAAGATTCATCGCGATCGTAGGCAGATGGTAAAGGGCGGTGGTTCTAGTCAGTTGAGTGTAAAACAACTGCAGCAACGAGTTGGTGTAAAACCCAGTCTTGCGGATTGCTTGGATGGGCTTATGCTTCTTCAGGACATGCATTGCTCTGAGTACCTTTTGAAGTCATCATTGGTTACAGCACTCTCACAACTTACCTTGAAACCCAGTGCCAGTGATTTGGGTGCACTCCAGCAGCTCTTGGTTGATCAGCCTAACATCCCCAAGGAGGAAGTCCAATTCATCTTTGATATCATATTTGCCGAAGACATTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CK2S

Protein Analysis

200

Amino Acids

22.53

Weight (kDa)

7.62

Isoelectric Point (pI)

60.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 121
Acc36I ACCTGC 1 cut(s) 121
AccII CGCG 1 cut(s) 297
AciI CCGC 2 cut(s) 321, 386
AcuI CTGAAG 2 cut(s) 59, 398
AfaI GTAC 1 cut(s) 437
AfiI CCNNNNNNNGG 1 cut(s) 550
AgsI TTSAA 5 cut(s) 140, 166, 185, 445, 484
AjuI GAANNNNNNNTTGG 2 cut(s) 437, 469
AloI GAACNNNNNNTCC 2 cut(s) 95, 127
AluBI AGCT 2 cut(s) 63, 520
AluI AGCT 2 cut(s) 63, 520
Alw21I GWGCWC 1 cut(s) 511
Alw26I GTCTC 1 cut(s) 282
Alw44I GTGCAC 1 cut(s) 507
AoxI GGCC 2 cut(s) 6, 66
ApaLI GTGCAC 1 cut(s) 507
ApeKI GCWGC 2 cut(s) 355, 517
Asp700I GAANNNNTTC 1 cut(s) 258
AspS9I GGNCC 1 cut(s) 66
AsuHPI GGTGA 2 cut(s) 25, 55
BaeGI GKGCMC 1 cut(s) 511
BarI GAAGNNNNNNTAC 2 cut(s) 177, 209
BbsI GAAGAC 1 cut(s) 594
Bbv12I GWGCWC 1 cut(s) 511
BbvI GCAGC 2 cut(s) 367, 529
BccI CCATC 5 cut(s) 49, 122, 202, 304, 392
BceAI ACGGC 2 cut(s) 53, 172
BclI TGATCA 1 cut(s) 529
BcoDI GTCTC 1 cut(s) 282
BfaI CTAG 1 cut(s) 330
BfmI CTRYAG 1 cut(s) 353
BfuAI ACCTGC 1 cut(s) 121
BisI GCNGC 2 cut(s) 356, 518
BlsI GCNGC 2 cut(s) 357, 519
BmgT120I GGNCC 1 cut(s) 66
BmrI ACTGGG 2 cut(s) 372, 483
BmuI ACTGGG 2 cut(s) 372, 483
BpiI GAAGAC 1 cut(s) 594
BpmI CTGGAG 1 cut(s) 497
BsaJI CCNNGG 3 cut(s) 9, 249, 546
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 1 cut(s) 550
Bse1I ACTGG 4 cut(s) 201, 378, 489, 495
Bse3DI GCAATG 1 cut(s) 424
BseDI CCNNGG 3 cut(s) 9, 249, 546
BseGI GGATG 4 cut(s) 55, 117, 403, 540
BseLI CCNNNNNNNGG 1 cut(s) 550
BseMI GCAATG 1 cut(s) 424
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 4 cut(s) 201, 378, 489, 495
BseRI GAGGAG 1 cut(s) 100
BseSI GKGCMC 1 cut(s) 511
BseXI GCAGC 2 cut(s) 367, 529
BseYI CCCAGC 1 cut(s) 63
Bsh1236I CGCG 1 cut(s) 297
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 2 cut(s) 8, 68
BsiEI CGRYCG 1 cut(s) 301
BsiHKAI GWGCWC 1 cut(s) 511
BslI CCNNNNNNNGG 1 cut(s) 550
BsmAI GTCTC 1 cut(s) 282
BsnI GGCC 2 cut(s) 8, 68
Bsp1286I GDGCHC 1 cut(s) 511
Bsp143I GATC 2 cut(s) 298, 529
Bsp19I CCATGG 2 cut(s) 9, 249
Bsp68I TCGCGA 1 cut(s) 297
BspACI CCGC 2 cut(s) 321, 386
BspANI GGCC 2 cut(s) 8, 68
BspCNI CTCAG 1 cut(s) 424
BspFNI CGCG 1 cut(s) 297
BspMAI CTGCAG 1 cut(s) 357
BspMI ACCTGC 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 424
BsrI ACTGG 4 cut(s) 201, 378, 489, 495
BssECI CCNNGG 3 cut(s) 9, 249, 546
BssMI GATC 2 cut(s) 298, 529
BssT1I CCWWGG 3 cut(s) 9, 249, 546
Bst6I CTCTTC 2 cut(s) 180, 249
BstDEI CTNAG 1 cut(s) 432
BstDSI CCRYGG 2 cut(s) 9, 249
BstF5I GGATG 4 cut(s) 55, 117, 403, 540
BstFNI CGCG 1 cut(s) 297
BstKTI GATC 2 cut(s) 301, 532
BstMAI GTCTC 1 cut(s) 282
BstMBI GATC 2 cut(s) 298, 529
BstMCI CGRYCG 1 cut(s) 301
BstMWI GCNNNNNNNGC 1 cut(s) 197
BstNSI RCATGY 1 cut(s) 424
BstSFI CTRYAG 1 cut(s) 353
BstSLI GKGCMC 1 cut(s) 511
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 2 cut(s) 367, 529
BstV2I GAAGAC 1 cut(s) 594
BstXI CCANNNNNNTGG 1 cut(s) 502
BsuRI GGCC 2 cut(s) 8, 68
BtgI CCRYGG 2 cut(s) 9, 249
BtgZI GCGATG 1 cut(s) 278
BtsCI GGATG 4 cut(s) 55, 117, 403, 540
BtsIMutI CAGTG 2 cut(s) 496, 502
BtuMI TCGCGA 1 cut(s) 297
BveI ACCTGC 1 cut(s) 121
Cfr13I GGNCC 1 cut(s) 66
Csp6I GTAC 1 cut(s) 436
CviAII CATG 5 cut(s) 10, 238, 250, 263, 421
CviJI RGCY 7 cut(s) 8, 63, 68, 159, 403, 520, 535
CviKI_1 RGCY 7 cut(s) 8, 63, 68, 159, 403, 520, 535
CviQI GTAC 1 cut(s) 436
DdeI CTNAG 1 cut(s) 432
DpnI GATC 2 cut(s) 300, 531
DpnII GATC 2 cut(s) 298, 529
Eam1104I CTCTTC 2 cut(s) 180, 249
EarI CTCTTC 2 cut(s) 180, 249
Eco130I CCWWGG 3 cut(s) 9, 249, 546
Eco32I GATATC 1 cut(s) 574
Eco57I CTGAAG 2 cut(s) 59, 398
EcoRV GATATC 1 cut(s) 574
EcoT14I CCWWGG 3 cut(s) 9, 249, 546
EcoT22I ATGCAT 1 cut(s) 426
ErhI CCWWGG 3 cut(s) 9, 249, 546
FaeI CATG 5 cut(s) 13, 241, 253, 266, 424
FaiI YATR 7 cut(s) 11, 239, 251, 264, 407, 422, 578
FatI CATG 5 cut(s) 9, 237, 249, 262, 420
FbaI TGATCA 1 cut(s) 529
Fnu4HI GCNGC 2 cut(s) 356, 518
FokI GGATG 4 cut(s) 62, 104, 410, 527
Fsp4HI GCNGC 2 cut(s) 356, 518
FspBI CTAG 1 cut(s) 330
GluI GCNGC 2 cut(s) 356, 518
GsaI CCCAGC 1 cut(s) 67
GsuI CTGGAG 1 cut(s) 497
HaeIII GGCC 2 cut(s) 8, 68
Hin1II CATG 5 cut(s) 13, 241, 253, 266, 424
HinfI GANTC 3 cut(s) 143, 212, 289
HphI GGTGA 2 cut(s) 25, 55
Hpy166II GTNNAC 1 cut(s) 509
Hpy188I TCNGA 1 cut(s) 433
Hpy188III TCNNGA 6 cut(s) 37, 100, 121, 140, 296, 416
Hpy8I GTNNAC 1 cut(s) 509
HpyAV CCTTC 1 cut(s) 34
HpyCH4V TGCA 5 cut(s) 130, 220, 355, 424, 509
HpyF10VI GCNNNNNNNGC 1 cut(s) 197
HpyF3I CTNAG 1 cut(s) 432
Hsp92II CATG 5 cut(s) 13, 241, 253, 266, 424
Ksp22I TGATCA 1 cut(s) 529
Kzo9I GATC 2 cut(s) 298, 529
Lsp1109I GCAGC 2 cut(s) 367, 529
MaeI CTAG 1 cut(s) 330
MaeIII GTNAC 1 cut(s) 457
MalI GATC 2 cut(s) 300, 531
MboI GATC 2 cut(s) 298, 529
MboII GAAGA 7 cut(s) 28, 197, 253, 266, 298, 404, 599
MhlI GDGCHC 1 cut(s) 511
MluCI AATT 2 cut(s) 151, 560
MlyI GAGTC 1 cut(s) 206
MnlI CCTC 7 cut(s) 24, 39, 117, 118, 121, 181, 544
Mph1103I ATGCAT 1 cut(s) 426
MroXI GAANNNNTTC 1 cut(s) 258
MspA1I CMGCKG 1 cut(s) 63
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 1 cut(s) 197
NcoI CCATGG 2 cut(s) 9, 249
NdeII GATC 2 cut(s) 298, 529
NlaIII CATG 5 cut(s) 13, 241, 253, 266, 424
NruI TCGCGA 1 cut(s) 297
NsiI ATGCAT 1 cut(s) 426
NspI RCATGY 1 cut(s) 424
PaqCI CACCTGC 1 cut(s) 121
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 2 cut(s) 143, 289
PkrI GCNGC 2 cut(s) 357, 519
Ple19I CGATCG 1 cut(s) 301
PleI GAGTC 1 cut(s) 206
PpsI GAGTC 1 cut(s) 206
PspFI CCCAGC 1 cut(s) 63
PspPI GGNCC 1 cut(s) 66
PstI CTGCAG 1 cut(s) 357
PvuI CGATCG 1 cut(s) 301
PvuII CAGCTG 1 cut(s) 63
RruI TCGCGA 1 cut(s) 297
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
SatI GCNGC 2 cut(s) 356, 518
Sau3AI GATC 2 cut(s) 298, 529
Sau96I GGNCC 1 cut(s) 66
SchI GAGTC 1 cut(s) 206
SduI GDGCHC 1 cut(s) 511
SetI ASST 8 cut(s) 35, 45, 65, 128, 135, 441, 482, 522
SfcI CTRYAG 1 cut(s) 353
Sse9I AATT 2 cut(s) 151, 560
SsiI CCGC 2 cut(s) 321, 386
SspMI CTAG 1 cut(s) 330
StyI CCWWGG 3 cut(s) 9, 249, 546
TasI AATT 2 cut(s) 151, 560
TfiI GAWTC 2 cut(s) 143, 289
TscAI CASTG 2 cut(s) 496, 502
TseI GCWGC 2 cut(s) 355, 517
TspDTI ATGAA 4 cut(s) 251, 254, 281, 553
TspRI CASTG 2 cut(s) 496, 502
VneI GTGCAC 1 cut(s) 507
XceI RCATGY 1 cut(s) 424
XmnI GAANNNNTTC 1 cut(s) 258
XspI CTAG 1 cut(s) 330
Zsp2I ATGCAT 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.