Rh3BG277600

Casein Kinase 2 substrate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002014
Physical Location & Seq
Reverse (-)
27243467 .. 27255687
12221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG277600.1

Sequence Viewer

Length: 762 bp
ATGGAAGTGATGGTGAAGAAATACCAGAATAGGTTCAGGAATGTGAGGGATGAGATGGATTGTTGGGACAAGCTTCAAGTTCTCTTAGTTTCTCAGTTCAGAAACGCCTCCACCATCATCCAGAGGTTGCAGGTTCTTCAAGATTCCAAATACTATGGTGGCTTGAATGAACTTGTTGATATTCAAGAGGTGGTGTTGGTGAAGCAGATGGAGTCTTTGCAAAAACTCTTGCTCTCCATGAAAAATACCATGGAAGAGTTTCACTGCATTGTAATGTCTCTTGGGACGATTCATCGTGATGGTAGGCACATGATAAAAGGTGGTTCTAGTCAGCTGAGTACAAAACAACTACAGCAGCGAGTTGGTGTAAAACCCACTCTTGCAGATTGCTTAGATGGGCTTATGCTTCTCCAGGACATGCATTGCTCTGAGCACATTTTGAAGTCGTCATTAGTTTCAGCTCTATCAGCACTTACCTTTAAAGTGAGTACCAGTGATTTGAGTTCGCTGCAGCAAATCTTGATTGATCAGCCTAACATTCCCAGGGAAGAAGCTGTGTTTTGGCTGGATTATGGGATTGATCGTTGTACGATTATGGATGCTTTTTGCCTTGTTCTCCTATGGGAGGCGGGATATTGGGGATCAGGATCTCTGGGATCCATGTCGACATGGGCTAGCGGTGCTTTCAATTGTCGGCGATTGGATCTGGAAACTAGGCGGCTACGGCCTGCTAGGGTGGTGGATGATGATGGCAGGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CK2S

Protein Analysis

253

Amino Acids

28.84

Weight (kDa)

6.52

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CA109-like PF15011 7 - 172 1.9e-46 Ribosome biogenesis protein C1orf109-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 121
AccI GTMKAC 1 cut(s) 665
AciI CCGC 3 cut(s) 629, 678, 718
AclWI GGATC 5 cut(s) 649, 651, 655, 664, 711
AfaI GTAC 3 cut(s) 340, 490, 589
AfiI CCNNNNNNNGG 1 cut(s) 625
AgsI TTSAA 6 cut(s) 77, 140, 166, 185, 442, 688
AjnI CCWGG 2 cut(s) 411, 542
AluBI AGCT 4 cut(s) 73, 334, 461, 554
AluI AGCT 4 cut(s) 73, 334, 461, 554
Alw21I GWGCWC 1 cut(s) 435
Alw26I GTCTC 1 cut(s) 282
AlwI GGATC 5 cut(s) 649, 651, 655, 664, 711
AoxI GGCC 1 cut(s) 725
ApeKI GCWGC 3 cut(s) 355, 508, 511
Asp700I GAANNNNTTC 2 cut(s) 32, 258
AsuHPI GGTGA 2 cut(s) 25, 211
AsuNHI GCTAGC 1 cut(s) 674
BamHI GGATCC 1 cut(s) 656
Bbv12I GWGCWC 1 cut(s) 435
BbvI GCAGC 3 cut(s) 367, 495, 523
BccI CCATC 7 cut(s) 4, 49, 122, 202, 293, 389, 743
BceAI ACGGC 1 cut(s) 740
BciT130I CCWGG 2 cut(s) 413, 544
BclI TGATCA 1 cut(s) 526
BcoDI GTCTC 1 cut(s) 282
BfaI CTAG 4 cut(s) 327, 675, 714, 732
BfmI CTRYAG 2 cut(s) 350, 509
BfuAI ACCTGC 1 cut(s) 121
BisI GCNGC 4 cut(s) 356, 509, 512, 719
BlsI GCNGC 4 cut(s) 357, 510, 513, 720
Bme1390I CCNGG 2 cut(s) 413, 544
BmiI GGNNCC 1 cut(s) 658
BmrFI CCNGG 2 cut(s) 413, 544
BmsI GCATC 1 cut(s) 589
BmtI GCTAGC 1 cut(s) 678
BpmI CTGGAG 1 cut(s) 395
BsaBI GATNNNNATC 1 cut(s) 646
BsaJI CCNNGG 3 cut(s) 249, 542, 543
Bsc4I CCNNNNNNNGG 1 cut(s) 625
Bse1I ACTGG 1 cut(s) 492
Bse3DI GCAATG 1 cut(s) 421
Bse8I GATNNNNATC 1 cut(s) 646
BseBI CCWGG 2 cut(s) 413, 544
BseDI CCNNGG 3 cut(s) 249, 542, 543
BseGI GGATG 4 cut(s) 55, 117, 604, 748
BseJI GATNNNNATC 1 cut(s) 646
BseLI CCNNNNNNNGG 1 cut(s) 625
BseMI GCAATG 1 cut(s) 421
BseMII CTCAG 3 cut(s) 107, 326, 420
BseNI ACTGG 1 cut(s) 492
BseXI GCAGC 3 cut(s) 367, 495, 523
BshFI GGCC 1 cut(s) 727
BsiHKAI GWGCWC 1 cut(s) 435
BslFI GGGAC 2 cut(s) 80, 298
BslI CCNNNNNNNGG 1 cut(s) 625
BsmAI GTCTC 1 cut(s) 282
BsmFI GGGAC 2 cut(s) 80, 298
BsnI GGCC 1 cut(s) 727
Bsp1286I GDGCHC 1 cut(s) 435
Bsp143I GATC 6 cut(s) 526, 580, 641, 647, 656, 703
Bsp19I CCATGG 1 cut(s) 249
BspACI CCGC 3 cut(s) 629, 678, 718
BspANI GGCC 1 cut(s) 727
BspCNI CTCAG 3 cut(s) 106, 327, 421
BspLI GGNNCC 1 cut(s) 658
BspMAI CTGCAG 1 cut(s) 513
BspMI ACCTGC 1 cut(s) 121
BspOI GCTAGC 1 cut(s) 678
BspPI GGATC 5 cut(s) 649, 651, 655, 664, 711
BsrDI GCAATG 1 cut(s) 421
BsrI ACTGG 1 cut(s) 492
BssECI CCNNGG 3 cut(s) 249, 542, 543
BssMI GATC 6 cut(s) 526, 580, 641, 647, 656, 703
BssT1I CCWWGG 1 cut(s) 249
Bst2UI CCWGG 2 cut(s) 413, 544
Bst6I CTCTTC 1 cut(s) 249
BstC8I GCNNGC 2 cut(s) 676, 729
BstDEI CTNAG 5 cut(s) 85, 93, 335, 391, 429
BstDSI CCRYGG 1 cut(s) 249
BstENI CCTNNNNNAGG 1 cut(s) 623
BstF5I GGATG 4 cut(s) 55, 117, 604, 748
BstKTI GATC 6 cut(s) 529, 583, 644, 650, 659, 706
BstMAI GTCTC 1 cut(s) 282
BstMBI GATC 6 cut(s) 526, 580, 641, 647, 656, 703
BstMWI GCNNNNNNNGC 3 cut(s) 467, 680, 724
BstNI CCWGG 2 cut(s) 413, 544
BstNSI RCATGY 1 cut(s) 421
BstSCI CCNGG 2 cut(s) 411, 542
BstSFI CTRYAG 2 cut(s) 350, 509
BstV1I GCAGC 3 cut(s) 367, 495, 523
BstX2I RGATCY 3 cut(s) 647, 656, 703
BstYI RGATCY 3 cut(s) 647, 656, 703
BsuRI GGCC 1 cut(s) 727
BtgI CCRYGG 1 cut(s) 249
BtsCI GGATG 4 cut(s) 55, 117, 604, 748
BtsI GCAGTG 1 cut(s) 262
BtsIMutI CAGTG 2 cut(s) 262, 499
BveI ACCTGC 1 cut(s) 121
Cac8I GCNNGC 2 cut(s) 676, 729
Csp6I GTAC 3 cut(s) 339, 489, 588
CviAII CATG 6 cut(s) 238, 250, 310, 418, 661, 669
CviQI GTAC 3 cut(s) 339, 489, 588
DdeI CTNAG 5 cut(s) 85, 93, 335, 391, 429
DpnI GATC 6 cut(s) 528, 582, 643, 649, 658, 705
DpnII GATC 6 cut(s) 526, 580, 641, 647, 656, 703
DraI TTTAAA 1 cut(s) 481
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 249
EcoNI CCTNNNNNAGG 1 cut(s) 623
EcoRII CCWGG 2 cut(s) 411, 542
EcoT14I CCWWGG 1 cut(s) 249
EcoT22I ATGCAT 1 cut(s) 423
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 6 cut(s) 241, 253, 313, 421, 664, 672
FaqI GGGAC 2 cut(s) 80, 298
FatI CATG 6 cut(s) 237, 249, 309, 417, 660, 668
FauI CCCGC 1 cut(s) 622
FbaI TGATCA 1 cut(s) 526
FblI GTMKAC 1 cut(s) 665
Fnu4HI GCNGC 4 cut(s) 356, 509, 512, 719
FokI GGATG 4 cut(s) 62, 104, 611, 755
Fsp4HI GCNGC 4 cut(s) 356, 509, 512, 719
FspBI CTAG 4 cut(s) 327, 675, 714, 732
GluI GCNGC 4 cut(s) 356, 509, 512, 719
GsuI CTGGAG 1 cut(s) 395
HaeIII GGCC 1 cut(s) 727
Hin1II CATG 6 cut(s) 241, 253, 313, 421, 664, 672
HincII GTYRAC 1 cut(s) 666
HindII GTYRAC 1 cut(s) 666
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 3 cut(s) 143, 212, 289
HphI GGTGA 2 cut(s) 25, 211
Hpy166II GTNNAC 1 cut(s) 666
Hpy188I TCNGA 2 cut(s) 101, 430
Hpy188III TCNNGA 8 cut(s) 37, 121, 140, 185, 296, 520, 645, 707
Hpy8I GTNNAC 1 cut(s) 666
HpyCH4V TGCA 6 cut(s) 130, 220, 267, 383, 421, 511
HpyF10VI GCNNNNNNNGC 3 cut(s) 467, 680, 724
HpyF3I CTNAG 5 cut(s) 85, 93, 335, 391, 429
Hsp92II CATG 6 cut(s) 241, 253, 313, 421, 664, 672
Ksp22I TGATCA 1 cut(s) 526
Kzo9I GATC 6 cut(s) 526, 580, 641, 647, 656, 703
Lsp1109I GCAGC 3 cut(s) 367, 495, 523
LweI GCATC 1 cut(s) 589
MaeI CTAG 4 cut(s) 327, 675, 714, 732
MalI GATC 6 cut(s) 528, 582, 643, 649, 658, 705
MboI GATC 6 cut(s) 526, 580, 641, 647, 656, 703
MboII GAAGA 4 cut(s) 28, 128, 266, 560
MfeI CAATTG 1 cut(s) 688
MflI RGATCY 3 cut(s) 647, 656, 703
MhlI GDGCHC 1 cut(s) 435
MluCI AATT 1 cut(s) 688
MlyI GAGTC 1 cut(s) 221
MnlI CCTC 5 cut(s) 39, 117, 118, 181, 619
Mph1103I ATGCAT 1 cut(s) 423
MroXI GAANNNNTTC 2 cut(s) 32, 258
MseI TTAA 1 cut(s) 480
MslI CAYNNNNRTG 2 cut(s) 272, 297
MspA1I CMGCKG 1 cut(s) 334
MspR9I CCNGG 2 cut(s) 413, 544
MunI CAATTG 1 cut(s) 688
MvaI CCWGG 2 cut(s) 413, 544
MwoI GCNNNNNNNGC 3 cut(s) 467, 680, 724
NcoI CCATGG 1 cut(s) 249
NdeII GATC 6 cut(s) 526, 580, 641, 647, 656, 703
NheI GCTAGC 1 cut(s) 674
NlaIII CATG 6 cut(s) 241, 253, 313, 421, 664, 672
NlaIV GGNNCC 1 cut(s) 658
NsiI ATGCAT 1 cut(s) 423
NspI RCATGY 1 cut(s) 421
PasI CCCWGGG 1 cut(s) 543
PdmI GAANNNNTTC 2 cut(s) 32, 258
PfeI GAWTC 2 cut(s) 143, 289
PfoI TCCNGGA 1 cut(s) 411
PkrI GCNGC 4 cut(s) 357, 510, 513, 720
PleI GAGTC 1 cut(s) 220
PpsI GAGTC 1 cut(s) 220
Psp6I CCWGG 2 cut(s) 411, 542
PspGI CCWGG 2 cut(s) 411, 542
PspN4I GGNNCC 1 cut(s) 658
PstI CTGCAG 1 cut(s) 513
PsuI RGATCY 3 cut(s) 647, 656, 703
PvuII CAGCTG 1 cut(s) 334
RsaI GTAC 3 cut(s) 340, 490, 589
RsaNI GTAC 3 cut(s) 339, 489, 588
RseI CAYNNNNRTG 2 cut(s) 272, 297
SalI GTCGAC 1 cut(s) 664
SaqAI TTAA 1 cut(s) 480
SatI GCNGC 4 cut(s) 356, 509, 512, 719
Sau3AI GATC 6 cut(s) 526, 580, 641, 647, 656, 703
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 2 cut(s) 413, 544
SduI GDGCHC 1 cut(s) 435
SfaNI GCATC 1 cut(s) 589
SfcI CTRYAG 2 cut(s) 350, 509
SmiMI CAYNNNNRTG 2 cut(s) 272, 297
Sse9I AATT 1 cut(s) 688
SsiI CCGC 3 cut(s) 629, 678, 718
SspMI CTAG 4 cut(s) 327, 675, 714, 732
StyD4I CCNGG 2 cut(s) 411, 542
StyI CCWWGG 1 cut(s) 249
TaqI TCGA 1 cut(s) 665
TasI AATT 1 cut(s) 688
TatI WGTACW 1 cut(s) 338
TauI GCSGC 1 cut(s) 721
TfiI GAWTC 2 cut(s) 143, 289
Tru1I TTAA 1 cut(s) 480
Tru9I TTAA 1 cut(s) 480
TscAI CASTG 2 cut(s) 269, 499
TseI GCWGC 3 cut(s) 355, 508, 511
TspDTI ATGAA 3 cut(s) 183, 254, 281
TspRI CASTG 2 cut(s) 269, 499
XagI CCTNNNNNAGG 1 cut(s) 623
XceI RCATGY 1 cut(s) 421
XmiI GTMKAC 1 cut(s) 665
XmnI GAANNNNTTC 2 cut(s) 32, 258
XspI CTAG 4 cut(s) 327, 675, 714, 732
Zsp2I ATGCAT 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.