Rh7DG297600

Casein Kinase 2 substrate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0002014
Physical Location & Seq
Forward (+)
33914408 .. 33918572
4165 bp
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UTR
Exon/CDS
Intron
Rh7DG297600.1

Sequence Viewer

Length: 489 bp
ATGGAGGCGATGGTGAAGAAATACCAGCAGAGGTTCAGGAAGGTTAGGGACGAGATGGAGACCTGGGCCCACCTTCAATCTCTCTTGATTTCCCAGTTCAGAATCGCCTCCTCCACCATCCTCAGGTTGCAGGTGCTTCAAGATTCCACCAACTACGGTGGTGGCTTGAATGGAGTTGCCCATATTCAAGAGGCCGTGCTGCTCAAGCAGATGGAGTCTTTGCAAAAACTCTTGCTTTCCATGAACAACACCATGCAACAGTTTCATCGTATTGTCTTGTCTCTTGGGAGGATTCATCGTGATGGTAGGCACATGGTGAAAGGTGGTTCTAGTCAGCTGACTGTCAAACAACTGCAGCAGCGAGTTGGTGTCAAACCCAGTCTCGCCGATTGCGTAGATGGGCTCATGCTTCTTCACGACATGCACTGCTCTGAGTATGTCACCTCTCTCTCTTTTGATTTGCGCATTGTTAAGTTTACCGAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CK2S

Protein Analysis

162

Amino Acids

18.57

Weight (kDa)

9.43

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CA109-like PF15011 7 - 147 1.8e-41 Ribosome biogenesis protein C1orf109-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 121
Acc16I TGCGCA 1 cut(s) 464
Acc36I ACCTGC 1 cut(s) 121
AdeI CACNNNGTG 1 cut(s) 316
AfiI CCNNNNNNNGG 1 cut(s) 123
AgsI TTSAA 4 cut(s) 77, 140, 169, 188
AjnI CCWGG 1 cut(s) 62
AluBI AGCT 1 cut(s) 337
AluI AGCT 1 cut(s) 337
Alw26I GTCTC 3 cut(s) 53, 285, 386
AoxI GGCC 2 cut(s) 66, 192
ApaI GGGCCC 1 cut(s) 70
ApeKI GCWGC 3 cut(s) 199, 355, 358
AspLEI GCGC 1 cut(s) 465
AspS9I GGNCC 2 cut(s) 66, 67
AsuHPI GGTGA 3 cut(s) 25, 328, 433
AxyI CCTNAGG 1 cut(s) 122
BaeGI GKGCMC 1 cut(s) 70
BanII GRGCYC 2 cut(s) 70, 405
BbvI GCAGC 3 cut(s) 186, 367, 370
BccI CCATC 6 cut(s) 4, 49, 125, 205, 296, 392
BceAI ACGGC 1 cut(s) 179
BciT130I CCWGG 1 cut(s) 64
BcoDI GTCTC 3 cut(s) 53, 285, 386
BfaI CTAG 1 cut(s) 330
BfmI CTRYAG 1 cut(s) 353
BfuAI ACCTGC 1 cut(s) 121
BisI GCNGC 3 cut(s) 200, 356, 359
BlsI GCNGC 3 cut(s) 201, 357, 360
Bme1390I CCNGG 1 cut(s) 64
BmgT120I GGNCC 2 cut(s) 66, 67
BmiI GGNNCC 1 cut(s) 68
BmrFI CCNGG 1 cut(s) 64
BmrI ACTGGG 2 cut(s) 88, 372
BmuI ACTGGG 2 cut(s) 88, 372
BpuEI CTTGAG 1 cut(s) 188
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 2 cut(s) 63, 480
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse1I ACTGG 2 cut(s) 94, 378
Bse21I CCTNAGG 1 cut(s) 122
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 2 cut(s) 63, 480
BseGI GGATG 1 cut(s) 117
BseLI CCNNNNNNNGG 1 cut(s) 123
BseMII CTCAG 2 cut(s) 136, 423
BseNI ACTGG 2 cut(s) 94, 378
BseRI GAGGAG 1 cut(s) 100
BseSI GKGCMC 1 cut(s) 70
BseXI GCAGC 3 cut(s) 186, 367, 370
BshFI GGCC 2 cut(s) 68, 194
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 1 cut(s) 123
BsmAI GTCTC 3 cut(s) 53, 285, 386
BsmFI GGGAC 1 cut(s) 62
BsnI GGCC 2 cut(s) 68, 194
Bso31I GGTCTC 1 cut(s) 53
Bsp120I GGGCCC 1 cut(s) 66
Bsp1286I GDGCHC 2 cut(s) 70, 405
BspANI GGCC 2 cut(s) 68, 194
BspCNI CTCAG 2 cut(s) 135, 424
BspLI GGNNCC 1 cut(s) 68
BspMAI CTGCAG 1 cut(s) 357
BspMI ACCTGC 1 cut(s) 121
BspTNI GGTCTC 1 cut(s) 53
BsrI ACTGG 2 cut(s) 94, 378
BssECI CCNNGG 2 cut(s) 63, 480
Bst2UI CCWGG 1 cut(s) 64
Bst4CI ACNGT 3 cut(s) 158, 261, 343
BstDEI CTNAG 2 cut(s) 122, 432
BstF5I GGATG 1 cut(s) 117
BstHHI GCGC 1 cut(s) 465
BstMAI GTCTC 3 cut(s) 53, 285, 386
BstMWI GCNNNNNNNGC 1 cut(s) 205
BstNI CCWGG 1 cut(s) 64
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 1 cut(s) 62
BstSFI CTRYAG 1 cut(s) 353
BstSLI GKGCMC 1 cut(s) 70
BstV1I GCAGC 3 cut(s) 186, 367, 370
Bsu36I CCTNAGG 1 cut(s) 122
BsuRI GGCC 2 cut(s) 68, 194
BtgZI GCGATG 1 cut(s) 23
BtsCI GGATG 1 cut(s) 117
BtsI GCAGTG 1 cut(s) 424
BtsIMutI CAGTG 1 cut(s) 424
BveI ACCTGC 1 cut(s) 121
CfoI GCGC 1 cut(s) 465
Cfr13I GGNCC 2 cut(s) 66, 67
CspCI CAANNNNNGTGG 2 cut(s) 139, 174
CviAII CATG 5 cut(s) 241, 253, 313, 406, 421
CviJI RGCY 5 cut(s) 68, 165, 194, 337, 403
CviKI_1 RGCY 5 cut(s) 68, 165, 194, 337, 403
DdeI CTNAG 2 cut(s) 122, 432
DraIII CACNNNGTG 1 cut(s) 316
Eco24I GRGCYC 2 cut(s) 70, 405
Eco31I GGTCTC 1 cut(s) 53
Eco81I CCTNAGG 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 62
EcoT38I GRGCYC 2 cut(s) 70, 405
FaeI CATG 5 cut(s) 244, 256, 316, 409, 424
FaiI YATR 7 cut(s) 183, 242, 254, 314, 407, 422, 438
FaqI GGGAC 1 cut(s) 62
FatI CATG 5 cut(s) 240, 252, 312, 405, 420
Fnu4HI GCNGC 3 cut(s) 200, 356, 359
FokI GGATG 1 cut(s) 104
FriOI GRGCYC 2 cut(s) 70, 405
Fsp4HI GCNGC 3 cut(s) 200, 356, 359
FspBI CTAG 1 cut(s) 330
FspI TGCGCA 1 cut(s) 464
GlaI GCGC 1 cut(s) 464
GluI GCNGC 3 cut(s) 200, 356, 359
HaeIII GGCC 2 cut(s) 68, 194
HhaI GCGC 1 cut(s) 465
Hin1II CATG 5 cut(s) 244, 256, 316, 409, 424
Hin6I GCGC 1 cut(s) 463
HinP1I GCGC 1 cut(s) 463
HinfI GANTC 4 cut(s) 102, 143, 215, 292
HphI GGTGA 3 cut(s) 25, 328, 433
Hpy166II GTNNAC 1 cut(s) 477
Hpy188I TCNGA 2 cut(s) 101, 433
Hpy188III TCNNGA 6 cut(s) 37, 85, 140, 188, 299, 416
Hpy8I GTNNAC 1 cut(s) 477
HpyAV CCTTC 2 cut(s) 34, 83
HpyCH4III ACNGT 3 cut(s) 158, 261, 343
HpyCH4V TGCA 5 cut(s) 130, 223, 256, 355, 424
HpyF10VI GCNNNNNNNGC 1 cut(s) 205
HpyF3I CTNAG 2 cut(s) 122, 432
Hsp92II CATG 5 cut(s) 244, 256, 316, 409, 424
HspAI GCGC 1 cut(s) 463
LpnPI CCDG 8 cut(s) 22, 38, 49, 76, 107, 109, 116, 391
Lsp1109I GCAGC 3 cut(s) 186, 367, 370
MaeI CTAG 1 cut(s) 330
MaeIII GTNAC 1 cut(s) 439
MboII GAAGA 2 cut(s) 28, 404
MhlI GDGCHC 2 cut(s) 70, 405
MlyI GAGTC 1 cut(s) 224
MnlI CCTC 8 cut(s) 24, 118, 121, 131, 184, 282, 454, 475
MseI TTAA 1 cut(s) 471
MslI CAYNNNNRTG 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 337
MspR9I CCNGG 1 cut(s) 64
MvaI CCWGG 1 cut(s) 64
MwoI GCNNNNNNNGC 1 cut(s) 205
NlaIII CATG 5 cut(s) 244, 256, 316, 409, 424
NlaIV GGNNCC 1 cut(s) 68
NmuCI GTSAC 1 cut(s) 439
NsbI TGCGCA 1 cut(s) 464
NspI RCATGY 1 cut(s) 424
PaqCI CACCTGC 1 cut(s) 121
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PfeI GAWTC 3 cut(s) 102, 143, 292
PkrI GCNGC 3 cut(s) 201, 357, 360
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
Psp6I CCWGG 1 cut(s) 62
PspGI CCWGG 1 cut(s) 62
PspN4I GGNNCC 1 cut(s) 68
PspOMI GGGCCC 1 cut(s) 66
PspPI GGNCC 2 cut(s) 66, 67
PstI CTGCAG 1 cut(s) 357
PvuII CAGCTG 1 cut(s) 337
RseI CAYNNNNRTG 1 cut(s) 300
SaqAI TTAA 1 cut(s) 471
SatI GCNGC 3 cut(s) 200, 356, 359
Sau96I GGNCC 2 cut(s) 66, 67
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 1 cut(s) 64
SduI GDGCHC 2 cut(s) 70, 405
SetI ASST 9 cut(s) 35, 45, 65, 75, 128, 135, 325, 339, 446
SfcI CTRYAG 1 cut(s) 353
SmiMI CAYNNNNRTG 1 cut(s) 300
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
SspMI CTAG 1 cut(s) 330
StyD4I CCNGG 1 cut(s) 62
TaaI ACNGT 3 cut(s) 158, 261, 343
TfiI GAWTC 3 cut(s) 102, 143, 292
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TscAI CASTG 1 cut(s) 431
TseFI GTSAC 1 cut(s) 439
TseI GCWGC 3 cut(s) 199, 355, 358
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 3 cut(s) 254, 257, 284
TspRI CASTG 1 cut(s) 431
XceI RCATGY 1 cut(s) 424
XspI CTAG 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.