FvH4_5g26840

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006497
Physical Location & Seq
Reverse (-)
18214922 .. 18215453
532 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g26840.t1

Sequence Viewer

Length: 240 bp
ATGAAATCATTGATGCTCTTTGTTCTCCTCACTAGCATGGTGGCATTGAACCATGGAGTCCAAACAAATAAGAAGTTTCTTCACCCTGGAGTAGTTGATCCTTGCCAAAGACCAGGAGGACCTCACCCAGGTTGTCTTCCTAAGGACAACAAAGCAAGTCAGACCGTACCTGGTGAGAGCACACCTGCTAATGCTTATCAGCGCGGTTGCTCAAAACTTAATCGTTGTCGCTCACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

80

Amino Acids

8.53

Weight (kDa)

9.76

Isoelectric Point (pI)

29.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 22 - 77 8.3e-07 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 193
Acc36I ACCTGC 1 cut(s) 193
AccII CGCG 1 cut(s) 204
AciI CCGC 1 cut(s) 204
AclWI GGATC 1 cut(s) 92
AfaI GTAC 1 cut(s) 168
AfiI CCNNNNNNNGG 1 cut(s) 128
AgsI TTSAA 1 cut(s) 49
AjnI CCWGG 4 cut(s) 85, 112, 127, 169
Alw21I GWGCWC 1 cut(s) 182
AlwI GGATC 1 cut(s) 92
AspLEI GCGC 1 cut(s) 204
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 3 cut(s) 74, 116, 185
AvaII GGWCC 1 cut(s) 119
AxyI CCTNAGG 1 cut(s) 141
BbsI GAAGAC 1 cut(s) 128
Bbv12I GWGCWC 1 cut(s) 182
BciT130I CCWGG 4 cut(s) 87, 114, 129, 171
BfaI CTAG 1 cut(s) 33
BfuAI ACCTGC 1 cut(s) 193
Bme1390I CCNGG 4 cut(s) 87, 114, 129, 171
Bme18I GGWCC 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 119
BmrFI CCNGG 4 cut(s) 87, 114, 129, 171
BmsI GCATC 1 cut(s) 3
BpiI GAAGAC 1 cut(s) 128
BpmI CTGGAG 1 cut(s) 108
BsaJI CCNNGG 3 cut(s) 52, 85, 127
Bsc4I CCNNNNNNNGG 1 cut(s) 128
Bse21I CCTNAGG 1 cut(s) 141
BseBI CCWGG 4 cut(s) 87, 114, 129, 171
BseDI CCNNGG 3 cut(s) 52, 85, 127
BseLI CCNNNNNNNGG 1 cut(s) 128
BseRI GAGGAG 1 cut(s) 17
Bsh1236I CGCG 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 182
BslI CCNNNNNNNGG 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 182
Bsp143I GATC 1 cut(s) 97
Bsp19I CCATGG 1 cut(s) 52
BspACI CCGC 1 cut(s) 204
BspFNI CGCG 1 cut(s) 204
BspMI ACCTGC 1 cut(s) 193
BspPI GGATC 1 cut(s) 92
BssECI CCNNGG 3 cut(s) 52, 85, 127
BssMI GATC 1 cut(s) 97
BssT1I CCWWGG 1 cut(s) 52
Bst2UI CCWGG 4 cut(s) 87, 114, 129, 171
Bst4CI ACNGT 2 cut(s) 166, 237
BstDEI CTNAG 1 cut(s) 141
BstDSI CCRYGG 1 cut(s) 52
BstENI CCTNNNNNAGG 1 cut(s) 126
BstFNI CGCG 1 cut(s) 204
BstHHI GCGC 1 cut(s) 204
BstKTI GATC 1 cut(s) 100
BstMBI GATC 1 cut(s) 97
BstNI CCWGG 4 cut(s) 87, 114, 129, 171
BstSCI CCNGG 4 cut(s) 85, 112, 127, 169
BstUI CGCG 1 cut(s) 204
BstV2I GAAGAC 1 cut(s) 128
Bsu36I CCTNAGG 1 cut(s) 141
BtgI CCRYGG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 233
BveI ACCTGC 1 cut(s) 193
CfoI GCGC 1 cut(s) 204
Cfr13I GGNCC 1 cut(s) 119
CsiI ACCWGGT 1 cut(s) 169
Csp6I GTAC 1 cut(s) 167
CviAII CATG 2 cut(s) 37, 53
CviQI GTAC 1 cut(s) 167
DdeI CTNAG 1 cut(s) 141
DpnI GATC 1 cut(s) 99
DpnII GATC 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 119
Eco81I CCTNAGG 1 cut(s) 141
EcoNI CCTNNNNNAGG 1 cut(s) 126
EcoO109I RGGNCCY 1 cut(s) 119
EcoRII CCWGG 4 cut(s) 85, 112, 127, 169
EcoT14I CCWWGG 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 52
FaeI CATG 2 cut(s) 40, 56
FaiI YATR 2 cut(s) 38, 54
FatI CATG 2 cut(s) 36, 52
FspBI CTAG 1 cut(s) 33
GlaI GCGC 1 cut(s) 203
GsuI CTGGAG 1 cut(s) 108
HhaI GCGC 1 cut(s) 204
Hin1II CATG 2 cut(s) 40, 56
Hin6I GCGC 1 cut(s) 202
HinP1I GCGC 1 cut(s) 202
HinfI GANTC 1 cut(s) 57
HphI GGTGA 3 cut(s) 74, 116, 185
Hpy188I TCNGA 1 cut(s) 162
HpyCH4III ACNGT 2 cut(s) 166, 237
HpyF3I CTNAG 1 cut(s) 141
Hsp92II CATG 2 cut(s) 40, 56
HspAI GCGC 1 cut(s) 202
Kzo9I GATC 1 cut(s) 97
LpnPI CCDG 9 cut(s) 72, 99, 99, 114, 126, 141, 156, 183, 198
LweI GCATC 1 cut(s) 3
MabI ACCWGGT 1 cut(s) 169
MaeI CTAG 1 cut(s) 33
MalI GATC 1 cut(s) 99
MboI GATC 1 cut(s) 97
MboII GAAGA 2 cut(s) 71, 128
MhlI GDGCHC 1 cut(s) 182
MlyI GAGTC 1 cut(s) 66
MnlI CCTC 3 cut(s) 38, 110, 132
MseI TTAA 1 cut(s) 219
MslI CAYNNNNRTG 1 cut(s) 35
MspR9I CCNGG 4 cut(s) 87, 114, 129, 171
MvaI CCWGG 4 cut(s) 87, 114, 129, 171
MvnI CGCG 1 cut(s) 204
NcoI CCATGG 1 cut(s) 52
NdeII GATC 1 cut(s) 97
NlaIII CATG 2 cut(s) 40, 56
PaqCI CACCTGC 1 cut(s) 193
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
PpuMI RGGWCCY 1 cut(s) 119
Psp5II RGGWCCY 1 cut(s) 119
Psp6I CCWGG 4 cut(s) 85, 112, 127, 169
PspGI CCWGG 4 cut(s) 85, 112, 127, 169
PspPI GGNCC 1 cut(s) 119
PspPPI RGGWCCY 1 cut(s) 119
RsaI GTAC 1 cut(s) 168
RsaNI GTAC 1 cut(s) 167
RseI CAYNNNNRTG 1 cut(s) 35
SaqAI TTAA 1 cut(s) 219
Sau3AI GATC 1 cut(s) 97
Sau96I GGNCC 1 cut(s) 119
SchI GAGTC 1 cut(s) 66
ScrFI CCNGG 4 cut(s) 87, 114, 129, 171
SduI GDGCHC 1 cut(s) 182
SetI ASST 4 cut(s) 124, 133, 172, 187
SexAI ACCWGGT 1 cut(s) 169
SfaNI GCATC 1 cut(s) 3
SinI GGWCC 1 cut(s) 119
SmiMI CAYNNNNRTG 1 cut(s) 35
SsiI CCGC 1 cut(s) 204
SspMI CTAG 1 cut(s) 33
StyD4I CCNGG 4 cut(s) 85, 112, 127, 169
StyI CCWWGG 1 cut(s) 52
TaaI ACNGT 2 cut(s) 166, 237
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TscAI CASTG 1 cut(s) 240
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 240
VpaK11BI GGWCC 1 cut(s) 119
XagI CCTNNNNNAGG 1 cut(s) 126
XspI CTAG 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.