Prupe.8G194700_v2.0.a1

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0006497
Physical Location & Seq
Reverse (-)
18747049 .. 18747270
222 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G194700.1

Sequence Viewer

Length: 222 bp
ATGAAGACATTCATCCTCTGTGTGCTCATTGTTGGCATGGTGGCGCTGAACGAAGGAGTTGAACCTAAGAGGGCCAGCATTAACCCTGGAGTTCTTGACCCATGCCTAAAACCAGGAGGGCCACATCCTGGTTGTAGTGGTCCCAGAGGAAGCAACGGTGCACGTAAACCCGTTAACCCCTACAACCGGGGTTGCTTGAAATTTAATCGGTGTCGTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

74

Amino Acids

7.72

Weight (kDa)

9.87

Isoelectric Point (pI)

13.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 128
AcsI RAATTY 1 cut(s) 200
AfiI CCNNNNNNNGG 2 cut(s) 128, 186
AgsI TTSAA 2 cut(s) 62, 199
AjnI CCWGG 3 cut(s) 85, 112, 127
Alw21I GWGCWC 2 cut(s) 27, 163
Alw44I GTGCAC 1 cut(s) 159
AoxI GGCC 2 cut(s) 72, 119
ApaLI GTGCAC 1 cut(s) 159
ApoI RAATTY 1 cut(s) 200
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 3 cut(s) 72, 119, 140
AsuC2I CCSGG 1 cut(s) 188
AvaII GGWCC 1 cut(s) 140
BaeGI GKGCMC 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 2 cut(s) 27, 163
BciT130I CCWGG 3 cut(s) 87, 114, 129
BcnI CCSGG 1 cut(s) 188
BfoI RGCGCY 1 cut(s) 47
Bme1390I CCNGG 4 cut(s) 87, 114, 129, 188
Bme18I GGWCC 1 cut(s) 140
BmgT120I GGNCC 3 cut(s) 72, 119, 140
BmiI GGNNCC 1 cut(s) 142
BmrFI CCNGG 4 cut(s) 87, 114, 129, 188
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 108
BpuMI CCSGG 1 cut(s) 188
BsaAI YACGTR 1 cut(s) 164
BsaJI CCNNGG 2 cut(s) 85, 187
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 186
BseBI CCWGG 3 cut(s) 87, 114, 129
BseDI CCNNGG 2 cut(s) 85, 187
BseGI GGATG 2 cut(s) 12, 124
BseLI CCNNNNNNNGG 2 cut(s) 128, 186
BseSI GKGCMC 1 cut(s) 163
BshFI GGCC 2 cut(s) 74, 121
BsiHKAI GWGCWC 2 cut(s) 27, 163
BsiSI CCGG 1 cut(s) 187
BslFI GGGAC 1 cut(s) 126
BslI CCNNNNNNNGG 2 cut(s) 128, 186
BsmFI GGGAC 1 cut(s) 126
BsnI GGCC 2 cut(s) 74, 121
Bsp1286I GDGCHC 2 cut(s) 27, 163
BspANI GGCC 2 cut(s) 74, 121
BspLI GGNNCC 1 cut(s) 142
BssECI CCNNGG 2 cut(s) 85, 187
Bst2UI CCWGG 3 cut(s) 87, 114, 129
Bst4CI ACNGT 1 cut(s) 158
BstBAI YACGTR 1 cut(s) 164
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 1 cut(s) 66
BstF5I GGATG 2 cut(s) 12, 124
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 1 cut(s) 46
BstNI CCWGG 3 cut(s) 87, 114, 129
BstSCI CCNGG 4 cut(s) 85, 112, 127, 186
BstSLI GKGCMC 1 cut(s) 163
BstV2I GAAGAC 1 cut(s) 11
BsuRI GGCC 2 cut(s) 74, 121
BtsCI GGATG 2 cut(s) 12, 124
Cac8I GCNNGC 1 cut(s) 76
CfoI GCGC 1 cut(s) 46
Cfr13I GGNCC 3 cut(s) 72, 119, 140
CviAII CATG 2 cut(s) 37, 102
CviJI RGCY 2 cut(s) 74, 121
CviKI_1 RGCY 2 cut(s) 74, 121
DdeI CTNAG 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 140
EcoRII CCWGG 3 cut(s) 85, 112, 127
FaeI CATG 2 cut(s) 40, 105
FaiI YATR 2 cut(s) 38, 103
FaqI GGGAC 1 cut(s) 126
FatI CATG 2 cut(s) 36, 101
FokI GGATG 1 cut(s) 111
GlaI GCGC 1 cut(s) 45
GsuI CTGGAG 1 cut(s) 108
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 2 cut(s) 74, 121
HapII CCGG 1 cut(s) 187
HhaI GCGC 1 cut(s) 46
Hin1II CATG 2 cut(s) 40, 105
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HpaI GTTAAC 1 cut(s) 175
HpaII CCGG 1 cut(s) 187
Hpy166II GTNNAC 3 cut(s) 161, 167, 175
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 3 cut(s) 161, 167, 175
HpyAV CCTTC 1 cut(s) 47
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4IV ACGT 1 cut(s) 163
HpyCH4V TGCA 1 cut(s) 161
HpyF3I CTNAG 1 cut(s) 66
HpySE526I ACGT 1 cut(s) 163
Hsp92II CATG 2 cut(s) 40, 105
HspAI GCGC 1 cut(s) 44
KspAI GTTAAC 1 cut(s) 175
LpnPI CCDG 9 cut(s) 72, 88, 99, 99, 114, 126, 141, 157, 200
MaeII ACGT 1 cut(s) 163
MboII GAAGA 1 cut(s) 16
MhlI GDGCHC 2 cut(s) 27, 163
MluCI AATT 1 cut(s) 200
MnlI CCTC 4 cut(s) 26, 63, 110, 140
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 3 cut(s) 81, 174, 204
MspI CCGG 1 cut(s) 187
MspR9I CCNGG 4 cut(s) 87, 114, 129, 188
MvaI CCWGG 3 cut(s) 87, 114, 129
NciI CCSGG 1 cut(s) 188
NlaIII CATG 2 cut(s) 40, 105
NlaIV GGNNCC 1 cut(s) 142
PdmI GAANNNNTTC 1 cut(s) 8
PflMI CCANNNNNTGG 1 cut(s) 128
Ppu21I YACGTR 1 cut(s) 164
Psp6I CCWGG 3 cut(s) 85, 112, 127
PspGI CCWGG 3 cut(s) 85, 112, 127
PspN4I GGNNCC 1 cut(s) 142
PspPI GGNCC 3 cut(s) 72, 119, 140
SaqAI TTAA 3 cut(s) 81, 174, 204
Sau96I GGNCC 3 cut(s) 72, 119, 140
ScrFI CCNGG 4 cut(s) 87, 114, 129, 188
SduI GDGCHC 2 cut(s) 27, 163
SetI ASST 2 cut(s) 67, 166
SinI GGWCC 1 cut(s) 140
Sse9I AATT 1 cut(s) 200
StyD4I CCNGG 4 cut(s) 85, 112, 127, 186
TaaI ACNGT 1 cut(s) 158
TaiI ACGT 1 cut(s) 166
TasI AATT 1 cut(s) 200
Tru1I TTAA 3 cut(s) 81, 174, 204
Tru9I TTAA 3 cut(s) 81, 174, 204
TspDTI ATGAA 1 cut(s) 17
Van91I CCANNNNNTGG 1 cut(s) 128
VneI GTGCAC 1 cut(s) 159
VpaK11BI GGWCC 1 cut(s) 140
XapI RAATTY 1 cut(s) 200
XmnI GAANNNNTTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.