Rroxscaffold_7G00184990

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0006497
Physical Location & Seq
Reverse (-)
24292230 .. 24292472
243 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00184990.1

Sequence Viewer

Length: 243 bp
ATGGGGCTATCAAACATGAAATCCTTGATCCTCTGTGTGCTCATCATTCACATGGTGGCGTTGAACCATGGAGTCACAGCAAATAACAGATTTATTGACCCCGGAGTTCTTGATCCTTGCCAAAGACCAGGCGGACCGCACCCGGGTTGTCATCCTAATAACAACAACGGTGCGCCAAAGCCTGCTAATCCTTATAGCCGCGGTTGTTCAAAAGTTACTCGTTGTCGATCAGTTCCACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

80

Amino Acids

8.54

Weight (kDa)

9.56

Isoelectric Point (pI)

26.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 25 - 76 2.2e-13 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 201
AciI CCGC 4 cut(s) 132, 137, 199, 201
AclWI GGATC 2 cut(s) 22, 107
AdeI CACNNNGTG 1 cut(s) 55
AfiI CCNNNNNNNGG 1 cut(s) 143
AgsI TTSAA 2 cut(s) 64, 210
AjnI CCWGG 1 cut(s) 127
Alw21I GWGCWC 1 cut(s) 42
AlwI GGATC 2 cut(s) 22, 107
Ama87I CYCGRG 1 cut(s) 142
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 1 cut(s) 134
AsuC2I CCSGG 3 cut(s) 102, 143, 144
AvaI CYCGRG 1 cut(s) 142
AvaII GGWCC 1 cut(s) 134
Bbv12I GWGCWC 1 cut(s) 42
BciT130I CCWGG 1 cut(s) 129
BcnI CCSGG 3 cut(s) 102, 143, 144
BisI GCNGC 1 cut(s) 199
BlsI GCNGC 1 cut(s) 200
Bme1390I CCNGG 4 cut(s) 102, 129, 143, 144
Bme18I GGWCC 1 cut(s) 134
BmeT110I CYCGRG 1 cut(s) 142
BmgT120I GGNCC 1 cut(s) 134
BmrFI CCNGG 4 cut(s) 102, 129, 143, 144
BpuMI CCSGG 3 cut(s) 102, 143, 144
BsaJI CCNNGG 4 cut(s) 67, 100, 142, 199
Bsc4I CCNNNNNNNGG 1 cut(s) 143
BseBI CCWGG 1 cut(s) 129
BseDI CCNNGG 4 cut(s) 67, 100, 142, 199
BseGI GGATG 1 cut(s) 151
BseLI CCNNNNNNNGG 1 cut(s) 143
Bsh1236I CGCG 1 cut(s) 201
BsiHKAI GWGCWC 1 cut(s) 42
BsiHKCI CYCGRG 1 cut(s) 142
BsiSI CCGG 2 cut(s) 102, 143
BslI CCNNNNNNNGG 1 cut(s) 143
BsoBI CYCGRG 1 cut(s) 142
Bsp1286I GDGCHC 1 cut(s) 42
Bsp143I GATC 3 cut(s) 27, 112, 227
Bsp19I CCATGG 1 cut(s) 67
BspACI CCGC 4 cut(s) 132, 137, 199, 201
BspFNI CGCG 1 cut(s) 201
BspPI GGATC 2 cut(s) 22, 107
BssECI CCNNGG 4 cut(s) 67, 100, 142, 199
BssMI GATC 3 cut(s) 27, 112, 227
BssT1I CCWWGG 1 cut(s) 67
Bst2UI CCWGG 1 cut(s) 129
Bst4CI ACNGT 2 cut(s) 170, 240
BstC8I GCNNGC 1 cut(s) 183
BstDSI CCRYGG 2 cut(s) 67, 199
BstF5I GGATG 1 cut(s) 151
BstFNI CGCG 1 cut(s) 201
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 3 cut(s) 30, 115, 230
BstMBI GATC 3 cut(s) 27, 112, 227
BstNI CCWGG 1 cut(s) 129
BstSCI CCNGG 4 cut(s) 100, 127, 141, 142
BstUI CGCG 1 cut(s) 201
BtgI CCRYGG 2 cut(s) 67, 199
BtsCI GGATG 1 cut(s) 151
Cac8I GCNNGC 1 cut(s) 183
CfoI GCGC 1 cut(s) 175
Cfr13I GGNCC 1 cut(s) 134
Cfr42I CCGCGG 1 cut(s) 202
Cfr9I CCCGGG 1 cut(s) 142
CpoI CGGWCCG 1 cut(s) 134
CspI CGGWCCG 1 cut(s) 134
CviAII CATG 3 cut(s) 16, 52, 68
CviJI RGCY 3 cut(s) 7, 181, 198
CviKI_1 RGCY 3 cut(s) 7, 181, 198
DpnI GATC 3 cut(s) 29, 114, 229
DpnII GATC 3 cut(s) 27, 112, 227
DraIII CACNNNGTG 1 cut(s) 55
EciI GGCGGA 1 cut(s) 147
Eco130I CCWWGG 1 cut(s) 67
Eco47I GGWCC 1 cut(s) 134
Eco88I CYCGRG 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 67
FaeI CATG 3 cut(s) 19, 55, 71
FaiI YATR 4 cut(s) 17, 53, 69, 195
FatI CATG 3 cut(s) 15, 51, 67
Fnu4HI GCNGC 1 cut(s) 199
FokI GGATG 1 cut(s) 138
Fsp4HI GCNGC 1 cut(s) 199
GlaI GCGC 1 cut(s) 174
GluI GCNGC 1 cut(s) 199
HapII CCGG 2 cut(s) 102, 143
HhaI GCGC 1 cut(s) 175
Hin1II CATG 3 cut(s) 19, 55, 71
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HinfI GANTC 1 cut(s) 72
HpaII CCGG 2 cut(s) 102, 143
Hpy188III TCNNGA 1 cut(s) 110
HpyCH4III ACNGT 2 cut(s) 170, 240
Hsp92II CATG 3 cut(s) 19, 55, 71
HspAI GCGC 1 cut(s) 173
KspI CCGCGG 1 cut(s) 202
Kzo9I GATC 3 cut(s) 27, 112, 227
LpnPI CCDG 5 cut(s) 114, 115, 141, 156, 195
MaeIII GTNAC 2 cut(s) 73, 214
MalI GATC 3 cut(s) 29, 114, 229
MboI GATC 3 cut(s) 27, 112, 227
MhlI GDGCHC 1 cut(s) 42
MlyI GAGTC 1 cut(s) 81
MnlI CCTC 1 cut(s) 41
MslI CAYNNNNRTG 1 cut(s) 50
MspA1I CMGCKG 1 cut(s) 201
MspI CCGG 2 cut(s) 102, 143
MspR9I CCNGG 4 cut(s) 102, 129, 143, 144
MvaI CCWGG 1 cut(s) 129
MvnI CGCG 1 cut(s) 201
NciI CCSGG 3 cut(s) 102, 143, 144
NcoI CCATGG 1 cut(s) 67
NdeII GATC 3 cut(s) 27, 112, 227
NlaIII CATG 3 cut(s) 19, 55, 71
NmuCI GTSAC 1 cut(s) 73
PkrI GCNGC 1 cut(s) 200
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
PspPI GGNCC 1 cut(s) 134
RseI CAYNNNNRTG 1 cut(s) 50
Rsr2I CGGWCCG 1 cut(s) 134
RsrII CGGWCCG 1 cut(s) 134
SacII CCGCGG 1 cut(s) 202
SatI GCNGC 1 cut(s) 199
Sau3AI GATC 3 cut(s) 27, 112, 227
Sau96I GGNCC 1 cut(s) 134
SchI GAGTC 1 cut(s) 81
ScrFI CCNGG 4 cut(s) 102, 129, 143, 144
SduI GDGCHC 1 cut(s) 42
Sfr303I CCGCGG 1 cut(s) 202
SgrBI CCGCGG 1 cut(s) 202
SinI GGWCC 1 cut(s) 134
SmaI CCCGGG 1 cut(s) 144
SmiMI CAYNNNNRTG 1 cut(s) 50
SsiI CCGC 4 cut(s) 132, 137, 199, 201
StyD4I CCNGG 4 cut(s) 100, 127, 141, 142
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 2 cut(s) 170, 240
TaqI TCGA 1 cut(s) 226
TauI GCSGC 1 cut(s) 201
TseFI GTSAC 1 cut(s) 73
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 1 cut(s) 32
TspMI CCCGGG 1 cut(s) 142
VpaK11BI GGWCC 1 cut(s) 134
XmaI CCCGGG 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.