Rorug06G0159100

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006497
Physical Location & Seq
Forward (+)
22717589 .. 22717969
381 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0159100.1

Sequence Viewer

Length: 279 bp
ATGAGCTCCTTTGGGAGGACTTGTGGCAGCAATATGAGCATTTCTGGGATGCGTTGTGGCAGCAATTTGAGTCGAATTAGTGGCTTAACTACTGGATGGCCATTTGCATTCAGATGCTATTTGAATAACCTGGTTGCTAACCAGGCTGATGACGAGGAGGTTGATGATGAAGAGCTGATGACGAGGAGGTTGATTATGAAGAGACCTTCAAGCACTTCGTTCTCCCTTTGTATGAATATAGGTTTACTTACAGAGTCACTATTTTTTATTTGGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

92

Amino Acids

10.33

Weight (kDa)

5.21

Isoelectric Point (pI)

57.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 98
AfiI CCNNNNNNNGG 1 cut(s) 15
AgsI TTSAA 2 cut(s) 124, 210
AjnI CCWGG 2 cut(s) 129, 141
AluBI AGCT 2 cut(s) 6, 175
AluI AGCT 2 cut(s) 6, 175
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 196
AoxI GGCC 1 cut(s) 98
ApeKI GCWGC 2 cut(s) 27, 60
BalI TGGCCA 1 cut(s) 100
BanII GRGCYC 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 2 cut(s) 39, 72
BccI CCATC 1 cut(s) 90
BciT130I CCWGG 2 cut(s) 131, 143
BcoDI GTCTC 1 cut(s) 196
BisI GCNGC 2 cut(s) 28, 61
BlsI GCNGC 2 cut(s) 29, 62
Bme1390I CCNGG 2 cut(s) 131, 143
BmrFI CCNGG 2 cut(s) 131, 143
BmsI GCATC 2 cut(s) 39, 104
BsaI GGTCTC 1 cut(s) 196
BsaXI ACNNNNNCTCC 2 cut(s) 7, 37
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse1I ACTGG 1 cut(s) 97
BseBI CCWGG 2 cut(s) 131, 143
BseGI GGATG 2 cut(s) 54, 101
BseLI CCNNNNNNNGG 1 cut(s) 15
BseNI ACTGG 1 cut(s) 97
BseRI GAGGAG 2 cut(s) 170, 199
BseXI GCAGC 2 cut(s) 39, 72
BshFI GGCC 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 15
BsmAI GTCTC 1 cut(s) 196
BsmI GAATGC 1 cut(s) 107
BsnI GGCC 1 cut(s) 100
Bso31I GGTCTC 1 cut(s) 196
Bsp1286I GDGCHC 1 cut(s) 8
BspANI GGCC 1 cut(s) 100
BspQI GCTCTTC 1 cut(s) 165
BspTNI GGTCTC 1 cut(s) 196
BsrI ACTGG 1 cut(s) 97
Bst2UI CCWGG 2 cut(s) 131, 143
Bst6I CTCTTC 2 cut(s) 165, 194
BstENI CCTNNNNNAGG 1 cut(s) 13
BstF5I GGATG 2 cut(s) 54, 101
BstMAI GTCTC 1 cut(s) 196
BstMWI GCNNNNNNNGC 2 cut(s) 36, 143
BstNI CCWGG 2 cut(s) 131, 143
BstSCI CCNGG 2 cut(s) 129, 141
BstV1I GCAGC 2 cut(s) 39, 72
BsuRI GGCC 1 cut(s) 100
BtsCI GGATG 2 cut(s) 54, 101
CsiI ACCWGGT 1 cut(s) 129
CviJI RGCY 5 cut(s) 6, 84, 100, 146, 175
CviKI_1 RGCY 5 cut(s) 6, 84, 100, 146, 175
EaeI YGGCCR 1 cut(s) 98
Eam1104I CTCTTC 2 cut(s) 165, 194
EarI CTCTTC 2 cut(s) 165, 194
Ecl136II GAGCTC 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 8
Eco31I GGTCTC 1 cut(s) 196
Eco53kI GAGCTC 1 cut(s) 6
EcoICRI GAGCTC 1 cut(s) 6
EcoNI CCTNNNNNAGG 1 cut(s) 13
EcoRII CCWGG 2 cut(s) 129, 141
EcoT38I GRGCYC 1 cut(s) 8
FaiI YATR 4 cut(s) 35, 197, 233, 239
Fnu4HI GCNGC 2 cut(s) 28, 61
FokI GGATG 2 cut(s) 61, 108
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 2 cut(s) 28, 61
GluI GCNGC 2 cut(s) 28, 61
HaeIII GGCC 1 cut(s) 100
HinfI GANTC 2 cut(s) 70, 254
Hpy166II GTNNAC 1 cut(s) 245
Hpy188I TCNGA 1 cut(s) 113
Hpy8I GTNNAC 1 cut(s) 245
HpyAV CCTTC 1 cut(s) 216
HpyCH4V TGCA 1 cut(s) 107
HpyF10VI GCNNNNNNNGC 2 cut(s) 36, 143
LguI GCTCTTC 1 cut(s) 165
LmnI GCTCC 1 cut(s) 11
LpnPI CCDG 6 cut(s) 30, 78, 116, 128, 143, 155
Lsp1109I GCAGC 2 cut(s) 39, 72
LweI GCATC 2 cut(s) 39, 104
MabI ACCWGGT 1 cut(s) 129
MaeIII GTNAC 1 cut(s) 255
MboII GAAGA 2 cut(s) 182, 211
MhlI GDGCHC 1 cut(s) 8
MlsI TGGCCA 1 cut(s) 100
MluCI AATT 2 cut(s) 64, 75
MluNI TGGCCA 1 cut(s) 100
MlyI GAGTC 2 cut(s) 79, 263
MnlI CCTC 5 cut(s) 9, 148, 151, 177, 180
Mox20I TGGCCA 1 cut(s) 100
MscI TGGCCA 1 cut(s) 100
MseI TTAA 2 cut(s) 86, 277
MslI CAYNNNNRTG 1 cut(s) 112
Msp20I TGGCCA 1 cut(s) 100
MspR9I CCNGG 2 cut(s) 131, 143
Mva1269I GAATGC 1 cut(s) 107
MvaI CCWGG 2 cut(s) 131, 143
MwoI GCNNNNNNNGC 2 cut(s) 36, 143
NmuCI GTSAC 1 cut(s) 255
PciSI GCTCTTC 1 cut(s) 165
PctI GAATGC 1 cut(s) 107
PkrI GCNGC 2 cut(s) 29, 62
PleI GAGTC 2 cut(s) 78, 262
PpsI GAGTC 2 cut(s) 78, 262
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 2 cut(s) 129, 141
PspGI CCWGG 2 cut(s) 129, 141
RseI CAYNNNNRTG 1 cut(s) 112
SacI GAGCTC 1 cut(s) 8
SapI GCTCTTC 1 cut(s) 165
SaqAI TTAA 2 cut(s) 86, 277
SatI GCNGC 2 cut(s) 28, 61
SchI GAGTC 2 cut(s) 79, 263
ScrFI CCNGG 2 cut(s) 131, 143
SduI GDGCHC 1 cut(s) 8
SetI ASST 7 cut(s) 8, 132, 162, 177, 191, 208, 244
SexAI ACCWGGT 1 cut(s) 129
SfaNI GCATC 2 cut(s) 39, 104
SmiMI CAYNNNNRTG 1 cut(s) 112
Sse9I AATT 2 cut(s) 64, 75
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 2 cut(s) 129, 141
TaqI TCGA 1 cut(s) 73
TasI AATT 2 cut(s) 64, 75
Tru1I TTAA 2 cut(s) 86, 277
Tru9I TTAA 2 cut(s) 86, 277
TseFI GTSAC 1 cut(s) 255
TseI GCWGC 2 cut(s) 27, 60
Tsp45I GTSAC 1 cut(s) 255
TspDTI ATGAA 3 cut(s) 183, 212, 248
XagI CCTNNNNNAGG 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.