RLG00000031333

Endonuclease 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006497
Physical Location & Seq
Reverse (-)
4172342 .. 4178400
6059 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031333

Sequence Viewer

Length: 1131 bp
ATGCGTGGTGGCTATGGCTGGTGGAGCGGTGATCAGTGTCGATGGTCAGTTGACCGTGGATGGGGACAGTGGTATGTTGGGGATAGTCAGGCCCTATGGCAAAGGGTTGATGGCTGGGTGAGTGGAGGGGGGTGGTGGTCTGGAGATGCGAAGATCAGCTGGGAACTTCTTGGTTTTCTAATGATTGCTCTTAGGCTGGTGTCGGAGCTTTGGGAAATGGAAGGAGAGGGCTGCGGCGACCTGGATTTTGTTCTGGGAGAGAGAGAGAAGAAGCAGGAGACTTTGACTCAATGCAAACCCGAAAGGGGACCATCTTTTAAGATATATGGGTTTGTTTGGGCTGTGGTGGAATGGAAGTGTTGGGGCAAAGAAGGCCACTATGCAGTTTGCAAGATAGCAGAGGGATATCTGAGTGAAGATGCTTTAGCTGCAGTGAGAGAATTGCTACCGGAATTTGTTGGAGGTGATCTTGCTGCAGTTTGCTCGTGGGCTGATCAGATTCGCTTTCATTATCATTGGAGTAGTGCTTTACACTATGTTGACACACCCGATTTTCTGTGTAACTACAATTACAGCAGAGACTGCCATGACTCTGCTGGACATAAGGGATGTGTAACTGGAGCAATATACAACTATACGATGCAACTTTTGACTTATCAGGAGTCCAACTCAAAATCAAAATGTACGCACACATATTTATTGGATACACCTTGCTATTCCAAATTATTAATATTCCTGAATATCAAAATCCTTCAGGTACTTCTTTTCTTGTCTCACTTTATTGGGGATATCCATCAGCTTCTACATGTTGGTTTCACCGGAGATCTAGGGTCGAACACAATAAGAGTCCGTTGGTATCCGCAGGAAAACAAATCTTCACCATTCTTTCTTTGTGGTTTTATCTTTTATGAGAATGATTGTGCAAAGCAACGACCAGATATTATAGATGCTTGTTCTGCTAATAGCATGAGGTATGCTTCTGAAAGCATTGGTTTGGCATGCAAATTTGCTTACAGAAATACCACTCCAGGAAGCACTCTAAATCGTATTTTCTTTTTTCTTCTTTCTAAACCGTATTATAGATTTCAAATCATCACTAACCTGTCCGTTAACAATAATAATCAGTTTTGA

Protein Analysis

377

Amino Acids

43.11

Weight (kDa)

6.07

Isoelectric Point (pI)

30.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 121 - 289 8.3e-39 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 27
AciI CCGC 3 cut(s) 27, 234, 860
AcsI RAATTY 2 cut(s) 452, 1004
AcuI CTGAAG 1 cut(s) 737
AfaI GTAC 2 cut(s) 685, 759
AfiI CCNNNNNNNGG 2 cut(s) 61, 305
AflIII ACRYGT 1 cut(s) 805
AgsI TTSAA 1 cut(s) 1088
AjnI CCWGG 2 cut(s) 240, 1027
AluBI AGCT 4 cut(s) 159, 208, 428, 799
AluI AGCT 4 cut(s) 159, 208, 428, 799
Alw26I GTCTC 3 cut(s) 272, 573, 777
AlwNI CAGNNNCTG 1 cut(s) 582
AoxI GGCC 2 cut(s) 90, 373
ApeKI GCWGC 3 cut(s) 231, 428, 473
ApoI RAATTY 2 cut(s) 452, 1004
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
AseI ATTAAT 1 cut(s) 728
AspS9I GGNCC 2 cut(s) 91, 308
AsuHPI GGTGA 5 cut(s) 41, 130, 476, 808, 870
AvaII GGWCC 1 cut(s) 308
BauI CACGAG 1 cut(s) 484
BbvI GCAGC 3 cut(s) 218, 415, 460
BccI CCATC 5 cut(s) 36, 54, 104, 319, 801
BcgI CGANNNNNNTGC 2 cut(s) 465, 499
BciT130I CCWGG 2 cut(s) 242, 1029
BciVI GTATCC 2 cut(s) 697, 867
BclI TGATCA 2 cut(s) 31, 493
BcoDI GTCTC 3 cut(s) 272, 573, 777
BfaI CTAG 1 cut(s) 827
BfmI CTRYAG 2 cut(s) 429, 474
BfuI GTATCC 2 cut(s) 697, 867
BglII AGATCT 1 cut(s) 823
BisI GCNGC 4 cut(s) 232, 235, 429, 474
BlsI GCNGC 4 cut(s) 233, 236, 430, 475
Bme1390I CCNGG 2 cut(s) 242, 1029
Bme18I GGWCC 1 cut(s) 308
BmgT120I GGNCC 2 cut(s) 91, 308
BmiI GGNNCC 1 cut(s) 309
BmrFI CCNGG 2 cut(s) 242, 1029
BmsI GCATC 4 cut(s) 136, 409, 630, 937
BplI GAGNNNNNCTC 2 cut(s) 653, 685
BpmI CTGGAG 3 cut(s) 162, 639, 1011
BsaBI GATNNNNATC 1 cut(s) 792
BsaJI CCNNGG 1 cut(s) 55
BsaWI WCCGGW 2 cut(s) 448, 818
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 305
Bse1I ACTGG 1 cut(s) 622
Bse8I GATNNNNATC 1 cut(s) 792
BseBI CCWGG 2 cut(s) 242, 1029
BseDI CCNNGG 1 cut(s) 55
BseGI GGATG 2 cut(s) 65, 614
BseJI GATNNNNATC 1 cut(s) 792
BseLI CCNNNNNNNGG 2 cut(s) 61, 305
BseMII CTCAG 1 cut(s) 401
BseNI ACTGG 1 cut(s) 622
BseXI GCAGC 3 cut(s) 218, 415, 460
BseYI CCCAGC 2 cut(s) 114, 159
BshFI GGCC 2 cut(s) 92, 375
BsiSI CCGG 2 cut(s) 449, 819
BslFI GGGAC 2 cut(s) 78, 321
BslI CCNNNNNNNGG 2 cut(s) 61, 305
BsmAI GTCTC 3 cut(s) 272, 573, 777
BsmFI GGGAC 2 cut(s) 78, 321
BsnI GGCC 2 cut(s) 92, 375
Bsp143I GATC 5 cut(s) 31, 153, 466, 493, 823
BspACI CCGC 3 cut(s) 27, 234, 860
BspANI GGCC 2 cut(s) 92, 375
BspCNI CTCAG 1 cut(s) 402
BspLI GGNNCC 1 cut(s) 309
BspMAI CTGCAG 2 cut(s) 433, 478
BsrBI CCGCTC 1 cut(s) 27
BsrI ACTGG 1 cut(s) 622
BssECI CCNNGG 1 cut(s) 55
BssMI GATC 5 cut(s) 31, 153, 466, 493, 823
BssSI CACGAG 1 cut(s) 484
Bst2BI CACGAG 1 cut(s) 484
Bst2UI CCWGG 2 cut(s) 242, 1029
Bst4CI ACNGT 3 cut(s) 56, 69, 1074
BstAPI GCANNNNNTGC 1 cut(s) 582
BstC8I GCNNGC 1 cut(s) 1000
BstDEI CTNAG 2 cut(s) 191, 410
BstDSI CCRYGG 1 cut(s) 55
BstF5I GGATG 2 cut(s) 65, 614
BstKTI GATC 5 cut(s) 34, 156, 469, 496, 826
BstMAI GTCTC 3 cut(s) 272, 573, 777
BstMBI GATC 5 cut(s) 31, 153, 466, 493, 823
BstMWI GCNNNNNNNGC 5 cut(s) 24, 372, 428, 582, 956
BstNI CCWGG 2 cut(s) 242, 1029
BstNSI RCATGY 2 cut(s) 809, 1002
BstSCI CCNGG 2 cut(s) 240, 1027
BstSFI CTRYAG 2 cut(s) 429, 474
BstV1I GCAGC 3 cut(s) 218, 415, 460
BstX2I RGATCY 1 cut(s) 823
BstYI RGATCY 1 cut(s) 823
BsuI GTATCC 2 cut(s) 697, 867
BsuRI GGCC 2 cut(s) 92, 375
BtgI CCRYGG 1 cut(s) 55
BtsCI GGATG 2 cut(s) 65, 614
BtsI GCAGTG 1 cut(s) 438
BtsIMutI CAGTG 3 cut(s) 41, 74, 438
Cac8I GCNNGC 1 cut(s) 1000
CaiI CAGNNNCTG 1 cut(s) 582
Cfr13I GGNCC 2 cut(s) 91, 308
Csp6I GTAC 2 cut(s) 684, 758
CviAII CATG 4 cut(s) 587, 806, 967, 999
CviQI GTAC 2 cut(s) 684, 758
DdeI CTNAG 2 cut(s) 191, 410
DpnI GATC 5 cut(s) 33, 155, 468, 495, 825
DpnII GATC 5 cut(s) 31, 153, 466, 493, 823
Eco32I GATATC 2 cut(s) 407, 790
Eco47I GGWCC 1 cut(s) 308
Eco57I CTGAAG 1 cut(s) 737
EcoO109I RGGNCCY 1 cut(s) 91
EcoRII CCWGG 2 cut(s) 240, 1027
EcoRV GATATC 2 cut(s) 407, 790
FaeI CATG 4 cut(s) 590, 809, 970, 1002
FaqI GGGAC 2 cut(s) 78, 321
FatI CATG 4 cut(s) 586, 805, 966, 998
FbaI TGATCA 2 cut(s) 31, 493
Fnu4HI GCNGC 4 cut(s) 232, 235, 429, 474
FokI GGATG 2 cut(s) 72, 621
Fsp4HI GCNGC 4 cut(s) 232, 235, 429, 474
FspBI CTAG 1 cut(s) 827
GluI GCNGC 4 cut(s) 232, 235, 429, 474
GsaI CCCAGC 2 cut(s) 118, 163
GsuI CTGGAG 3 cut(s) 162, 639, 1011
HaeIII GGCC 2 cut(s) 92, 375
HapII CCGG 2 cut(s) 449, 819
Hin1II CATG 4 cut(s) 590, 809, 970, 1002
HincII GTYRAC 3 cut(s) 52, 541, 1111
HindII GTYRAC 3 cut(s) 52, 541, 1111
HinfI GANTC 5 cut(s) 286, 499, 590, 662, 846
HpaI GTTAAC 1 cut(s) 1111
HpaII CCGG 2 cut(s) 449, 819
HphI GGTGA 5 cut(s) 41, 130, 476, 808, 870
Hpy166II GTNNAC 3 cut(s) 52, 541, 1111
Hpy188I TCNGA 4 cut(s) 205, 411, 498, 982
Hpy188III TCNNGA 3 cut(s) 141, 659, 736
Hpy8I GTNNAC 3 cut(s) 52, 541, 1111
HpyAV CCTTC 3 cut(s) 215, 365, 761
HpyCH4III ACNGT 3 cut(s) 56, 69, 1074
HpyCH4V TGCA 8 cut(s) 294, 383, 390, 431, 476, 643, 923, 1002
HpyF10VI GCNNNNNNNGC 5 cut(s) 24, 372, 428, 582, 956
HpyF3I CTNAG 2 cut(s) 191, 410
Hsp92II CATG 4 cut(s) 590, 809, 970, 1002
Ksp22I TGATCA 2 cut(s) 31, 493
KspAI GTTAAC 1 cut(s) 1111
Kzo9I GATC 5 cut(s) 31, 153, 466, 493, 823
LmnI GCTCC 3 cut(s) 24, 205, 620
Lsp1109I GCAGC 3 cut(s) 218, 415, 460
LweI GCATC 4 cut(s) 136, 409, 630, 937
MaeI CTAG 1 cut(s) 827
MaeIII GTNAC 2 cut(s) 560, 613
MalI GATC 5 cut(s) 33, 155, 468, 495, 825
MbiI CCGCTC 1 cut(s) 27
MboI GATC 5 cut(s) 31, 153, 466, 493, 823
MboII GAAGA 5 cut(s) 163, 280, 428, 867, 1052
MflI RGATCY 1 cut(s) 823
MluCI AATT 5 cut(s) 440, 452, 568, 722, 1004
MlyI GAGTC 4 cut(s) 280, 584, 671, 855
MmeI TCCRAC 3 cut(s) 183, 439, 690
MnlI CCTC 5 cut(s) 119, 220, 394, 455, 963
MseI TTAA 3 cut(s) 318, 728, 1110
MspA1I CMGCKG 1 cut(s) 159
MspI CCGG 2 cut(s) 449, 819
MspR9I CCNGG 2 cut(s) 242, 1029
MvaI CCWGG 2 cut(s) 242, 1029
MwoI GCNNNNNNNGC 5 cut(s) 24, 372, 428, 582, 956
NdeII GATC 5 cut(s) 31, 153, 466, 493, 823
NlaIII CATG 4 cut(s) 590, 809, 970, 1002
NlaIV GGNNCC 1 cut(s) 309
NspI RCATGY 2 cut(s) 809, 1002
PaeI GCATGC 1 cut(s) 1002
PciI ACATGT 1 cut(s) 805
PfeI GAWTC 1 cut(s) 499
PfoI TCCNGGA 1 cut(s) 1027
PkrI GCNGC 4 cut(s) 233, 236, 430, 475
PleI GAGTC 4 cut(s) 280, 584, 670, 854
PpsI GAGTC 4 cut(s) 280, 584, 670, 854
PscI ACATGT 1 cut(s) 805
PshBI ATTAAT 1 cut(s) 728
Psp6I CCWGG 2 cut(s) 240, 1027
PspFI CCCAGC 2 cut(s) 114, 159
PspGI CCWGG 2 cut(s) 240, 1027
PspN4I GGNNCC 1 cut(s) 309
PspPI GGNCC 2 cut(s) 91, 308
PstI CTGCAG 2 cut(s) 433, 478
PstNI CAGNNNCTG 1 cut(s) 582
PsuI RGATCY 1 cut(s) 823
PvuII CAGCTG 1 cut(s) 159
RsaI GTAC 2 cut(s) 685, 759
RsaNI GTAC 2 cut(s) 684, 758
SaqAI TTAA 3 cut(s) 318, 728, 1110
SatI GCNGC 4 cut(s) 232, 235, 429, 474
Sau3AI GATC 5 cut(s) 31, 153, 466, 493, 823
Sau96I GGNCC 2 cut(s) 91, 308
SchI GAGTC 4 cut(s) 280, 584, 671, 855
ScrFI CCNGG 2 cut(s) 242, 1029
SfaNI GCATC 4 cut(s) 136, 409, 630, 937
SfcI CTRYAG 2 cut(s) 429, 474
SinI GGWCC 1 cut(s) 308
SphI GCATGC 1 cut(s) 1002
Sse9I AATT 5 cut(s) 440, 452, 568, 722, 1004
SsiI CCGC 3 cut(s) 27, 234, 860
SspI AATATT 1 cut(s) 732
SspMI CTAG 1 cut(s) 827
StyD4I CCNGG 2 cut(s) 240, 1027
TaaI ACNGT 3 cut(s) 56, 69, 1074
TaqI TCGA 2 cut(s) 40, 833
TasI AATT 5 cut(s) 440, 452, 568, 722, 1004
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 1 cut(s) 499
Tru1I TTAA 3 cut(s) 318, 728, 1110
Tru9I TTAA 3 cut(s) 318, 728, 1110
TscAI CASTG 3 cut(s) 41, 74, 438
TseI GCWGC 3 cut(s) 231, 428, 473
TspDTI ATGAA 1 cut(s) 497
TspGWI ACGGA 2 cut(s) 839, 1096
TspRI CASTG 3 cut(s) 41, 74, 438
VpaK11BI GGWCC 1 cut(s) 308
VspI ATTAAT 1 cut(s) 728
XapI RAATTY 2 cut(s) 452, 1004
XceI RCATGY 2 cut(s) 809, 1002
XcmI CCANNNNNNNNNTGG 1 cut(s) 593
XspI CTAG 1 cut(s) 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.