FvH4_5g28030

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006934
Physical Location & Seq
Forward (+)
19276012 .. 19278671
2660 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g28030.t2

Sequence Viewer

Length: 1221 bp
ATGGAGTGGCGATCCATTAGGGGAAGCACATCAACGATGATTAGTTGTAGTAAATGCAACACTAGGATTCCTCGTCTAACGTCCGGTGTTGCTCAATATCGCTGCTCCTGCTGTGGTTACGTTACTCTGATGATTGAGACGACCAAGCCAGCTCTTGCACAGCACCACGATGAGGGTCAGCAGCAGCAGCAGCATAGCCATCGGAGGCGTGCTTCCAGCTGGATTTCGCATATGAAGGGACCATCACCATCATCTTCGTCGTCCAGGAACTCTAAATCCCCATCACCGTCATTGTCATCGCCATTGTCATCACCATCACCATCATCCTCCTCCTGGTCAACATCAACATCAACAACCAGCTTAATAAAAAGCTGCGGCAGAAAGCGCGCAGTTCTTTGCGGGGTGACATACAAGTCGCGCAAGTACAAGCTCAAAGGAACTCAGAATGATGTCAACAACATGAAAGACTTGTTGATCAAAACCTTTCATTTTCCATCCAAATGCATACGCGTACTCTCAGAAGAAGATGAGCAATTAGATAAAAATCTGATTCCGACAAAGAAGAACATCGAGAAATCCCTGAAATGGCTTGTGGAGGGGTGCCAGGCGGGAGATTCGTTGGTGTTCTACTTCTCAGGACACGGGCTACGGCAGCCCGATTTCAAAGACGACGAGATTGATGGTTTCGACGAGACTATTTGCCCTCTTGATTTCATGGAAGAGGGCATGATTCTTGACAATGATATCAATTCAACCATCGTTCACCCACTCAAGAAGGGTGTCACACTTCATGCTATCGTTGATGCTTGTCATAGTGGAACGATTCTTGATCTACTCTACGTCTACGACCACAGAGAGAACAAGTGGAAGGATAACAGTCCTCCTTCTCGTGCTATGAAAAGTACTAGTGGAGGGTTGGCAATCTGTCTCAGTGCCTGCGAAGACGATAAAATGGCCGCCGATACAACTGCTTTTAACCGAAATGTAATGAATGGAGCCATGACATACATTCTTATTGAAACTATTAAGAAACACTCTGAGAGTGGAATAACGTATGGGAATCTGCTCGACTCCATGCTTGATCAAATTCAAAGGATTAATGGGGAAAGATGCCTCAACTCCAGAATCTTGAGAAAAGTTTTCCGACACAAAATCATTCAGGAACCTTTATTGTCATCGTCTAAAGACTTCGATGTATACACCACGAAATTTCTCTTGTAA

Protein Analysis

407

Amino Acids

45.45

Weight (kDa)

8.89

Isoelectric Point (pI)

53.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 127 - 363 4.3e-31 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 412
AccB1I GGYRCC 1 cut(s) 600
AccI GTMKAC 2 cut(s) 843, 1197
AccII CGCG 3 cut(s) 387, 418, 510
AciI CCGC 4 cut(s) 375, 399, 608, 957
AclWI GGATC 1 cut(s) 6
AcoI YGGCCR 1 cut(s) 954
AcsI RAATTY 2 cut(s) 1086, 1208
AfaI GTAC 3 cut(s) 425, 513, 904
AfiI CCNNNNNNNGG 3 cut(s) 172, 333, 585
AflIII ACRYGT 1 cut(s) 508
AgsI TTSAA 4 cut(s) 664, 753, 1019, 1091
AhlI ACTAGT 1 cut(s) 905
AjnI CCWGG 3 cut(s) 263, 332, 603
AloI GAACNNNNNNTCC 2 cut(s) 260, 292
AluBI AGCT 5 cut(s) 152, 219, 360, 372, 430
AluI AGCT 5 cut(s) 152, 219, 360, 372, 430
Alw26I GTCTC 3 cut(s) 131, 686, 932
AlwI GGATC 1 cut(s) 6
AlwNI CAGNNNCTG 1 cut(s) 936
AoxI GGCC 1 cut(s) 954
ApeKI GCWGC 7 cut(s) 102, 181, 184, 187, 190, 372, 652
ApoI RAATTY 2 cut(s) 1086, 1208
AseI ATTAAT 1 cut(s) 1098
AspLEI GCGC 3 cut(s) 387, 389, 420
AspS9I GGNCC 1 cut(s) 239
AsuHPI GGTGA 6 cut(s) 237, 276, 303, 309, 415, 755
AvaII GGWCC 1 cut(s) 239
BanI GGYRCC 1 cut(s) 600
BauI CACGAG 1 cut(s) 888
BbsI GAAGAC 1 cut(s) 948
BbvI GCAGC 7 cut(s) 89, 193, 196, 199, 202, 359, 664
BccI CCATC 9 cut(s) 207, 250, 256, 289, 322, 328, 502, 674, 764
BceAI ACGGC 1 cut(s) 665
BcgI CGANNNNNNTGC 4 cut(s) 182, 216, 950, 984
BciT130I CCWGG 3 cut(s) 265, 334, 605
BclI TGATCA 2 cut(s) 474, 1081
BcoDI GTCTC 3 cut(s) 131, 686, 932
BcuI ACTAGT 1 cut(s) 905
BfaI CTAG 2 cut(s) 63, 906
BisI GCNGC 9 cut(s) 103, 182, 185, 188, 191, 373, 376, 653, 957
BlsI GCNGC 9 cut(s) 104, 183, 186, 189, 192, 374, 377, 654, 958
BmcAI AGTACT 1 cut(s) 904
Bme1390I CCNGG 3 cut(s) 265, 334, 605
Bme18I GGWCC 1 cut(s) 239
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 4 cut(s) 240, 602, 997, 1164
BmrFI CCNGG 3 cut(s) 265, 334, 605
BmsI GCATC 2 cut(s) 793, 1100
BpiI GAAGAC 1 cut(s) 948
BpmI CTGGAG 1 cut(s) 1105
BpuEI CTTGAG 2 cut(s) 755, 1150
BsaBI GATNNNNATC 1 cut(s) 543
BsaWI WCCGGW 1 cut(s) 83
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 333, 585
Bse8I GATNNNNATC 1 cut(s) 543
BseBI CCWGG 3 cut(s) 265, 334, 605
BseGI GGATG 2 cut(s) 323, 494
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 3 cut(s) 172, 333, 585
BseMII CTCAG 5 cut(s) 455, 531, 648, 943, 1029
BsePI GCGCGC 1 cut(s) 385
BseRI GAGGAG 1 cut(s) 319
BseXI GCAGC 7 cut(s) 89, 193, 196, 199, 202, 359, 664
Bsh1236I CGCG 3 cut(s) 387, 418, 510
BshFI GGCC 1 cut(s) 956
BshNI GGYRCC 1 cut(s) 600
BsiSI CCGG 1 cut(s) 84
BslFI GGGAC 1 cut(s) 252
BslI CCNNNNNNNGG 3 cut(s) 172, 333, 585
BsmAI GTCTC 3 cut(s) 131, 686, 932
BsmBI CGTCTC 1 cut(s) 131
BsmFI GGGAC 1 cut(s) 252
BsnI GGCC 1 cut(s) 956
Bsp143I GATC 4 cut(s) 11, 474, 829, 1081
BspACI CCGC 4 cut(s) 375, 399, 608, 957
BspANI GGCC 1 cut(s) 956
BspCNI CTCAG 5 cut(s) 454, 530, 647, 942, 1030
BspFNI CGCG 3 cut(s) 387, 418, 510
BspLI GGNNCC 4 cut(s) 240, 602, 997, 1164
BspPI GGATC 1 cut(s) 6
BspT107I GGYRCC 1 cut(s) 600
BssHII GCGCGC 1 cut(s) 385
BssMI GATC 4 cut(s) 11, 474, 829, 1081
BssNAI GTATAC 1 cut(s) 1198
BssSI CACGAG 1 cut(s) 888
Bst1107I GTATAC 1 cut(s) 1198
Bst2BI CACGAG 1 cut(s) 888
Bst2UI CCWGG 3 cut(s) 265, 334, 605
Bst4CI ACNGT 2 cut(s) 288, 878
Bst6I CTCTTC 1 cut(s) 714
BstC8I GCNNGC 4 cut(s) 150, 210, 387, 937
BstDEI CTNAG 5 cut(s) 441, 517, 634, 929, 1038
BstF5I GGATG 2 cut(s) 323, 494
BstFNI CGCG 3 cut(s) 387, 418, 510
BstHHI GCGC 3 cut(s) 387, 389, 420
BstKTI GATC 4 cut(s) 14, 477, 832, 1084
BstMAI GTCTC 3 cut(s) 131, 686, 932
BstMBI GATC 4 cut(s) 11, 474, 829, 1081
BstMWI GCNNNNNNNGC 5 cut(s) 108, 187, 190, 384, 652
BstNI CCWGG 3 cut(s) 265, 334, 605
BstSCI CCNGG 3 cut(s) 263, 332, 603
BstUI CGCG 3 cut(s) 387, 418, 510
BstV1I GCAGC 7 cut(s) 89, 193, 196, 199, 202, 359, 664
BstV2I GAAGAC 1 cut(s) 948
BstZ17I GTATAC 1 cut(s) 1198
BsuRI GGCC 1 cut(s) 956
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 2 cut(s) 323, 494
BtsIMutI CAGTG 1 cut(s) 937
Cac8I GCNNGC 4 cut(s) 150, 210, 387, 937
CaiI CAGNNNCTG 1 cut(s) 936
CfoI GCGC 3 cut(s) 387, 389, 420
Cfr13I GGNCC 1 cut(s) 239
Csp6I GTAC 3 cut(s) 424, 512, 903
CviAII CATG 6 cut(s) 460, 715, 727, 791, 1000, 1075
CviQI GTAC 3 cut(s) 424, 512, 903
DdeI CTNAG 5 cut(s) 441, 517, 634, 929, 1038
DpnI GATC 4 cut(s) 13, 476, 831, 1083
DpnII GATC 4 cut(s) 11, 474, 829, 1081
DrdI GACNNNNNNGTC 1 cut(s) 412
DseDI GACNNNNNNGTC 1 cut(s) 412
EaeI YGGCCR 1 cut(s) 954
Eam1104I CTCTTC 1 cut(s) 714
EarI CTCTTC 1 cut(s) 714
Eco32I GATATC 1 cut(s) 745
Eco47I GGWCC 1 cut(s) 239
EcoRII CCWGG 3 cut(s) 263, 332, 603
EcoRV GATATC 1 cut(s) 745
EcoT22I ATGCAT 1 cut(s) 506
Esp3I CGTCTC 1 cut(s) 131
FaeI CATG 6 cut(s) 463, 718, 730, 794, 1003, 1078
FaqI GGGAC 1 cut(s) 252
FatI CATG 6 cut(s) 459, 714, 726, 790, 999, 1074
FauI CCCGC 2 cut(s) 392, 601
FauNDI CATATG 1 cut(s) 231
FbaI TGATCA 2 cut(s) 474, 1081
FblI GTMKAC 2 cut(s) 843, 1197
Fnu4HI GCNGC 9 cut(s) 103, 182, 185, 188, 191, 373, 376, 653, 957
FokI GGATG 2 cut(s) 310, 481
Fsp4HI GCNGC 9 cut(s) 103, 182, 185, 188, 191, 373, 376, 653, 957
FspBI CTAG 2 cut(s) 63, 906
GlaI GCGC 3 cut(s) 386, 388, 419
GluI GCNGC 9 cut(s) 103, 182, 185, 188, 191, 373, 376, 653, 957
GsuI CTGGAG 1 cut(s) 1105
HaeIII GGCC 1 cut(s) 956
HapII CCGG 1 cut(s) 84
HhaI GCGC 3 cut(s) 387, 389, 420
Hin1II CATG 6 cut(s) 463, 718, 730, 794, 1003, 1078
Hin6I GCGC 3 cut(s) 385, 387, 418
HinP1I GCGC 3 cut(s) 385, 387, 418
HincII GTYRAC 2 cut(s) 339, 454
HindII GTYRAC 2 cut(s) 339, 454
HinfI GANTC 8 cut(s) 67, 550, 614, 730, 823, 1060, 1070, 1125
HpaII CCGG 1 cut(s) 84
HphI GGTGA 6 cut(s) 237, 276, 303, 309, 415, 755
Hpy166II GTNNAC 5 cut(s) 339, 454, 763, 844, 1198
Hpy188I TCNGA 8 cut(s) 129, 204, 444, 520, 549, 555, 1039, 1145
Hpy188III TCNNGA 9 cut(s) 571, 636, 707, 734, 772, 827, 1122, 1129, 1160
Hpy8I GTNNAC 5 cut(s) 339, 454, 763, 844, 1198
Hpy99I CGWCG 3 cut(s) 262, 674, 692
HpyAV CCTTC 4 cut(s) 229, 769, 862, 894
HpyCH4III ACNGT 2 cut(s) 288, 878
HpyCH4IV ACGT 4 cut(s) 80, 120, 840, 1052
HpyCH4V TGCA 3 cut(s) 57, 158, 504
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 187, 190, 384, 652
HpyF3I CTNAG 5 cut(s) 441, 517, 634, 929, 1038
HpySE526I ACGT 4 cut(s) 80, 120, 840, 1052
Hsp92II CATG 6 cut(s) 463, 718, 730, 794, 1003, 1078
HspAI GCGC 3 cut(s) 385, 387, 418
Ksp22I TGATCA 2 cut(s) 474, 1081
Kzo9I GATC 4 cut(s) 11, 474, 829, 1081
LmnI GCTCC 2 cut(s) 110, 995
Lsp1109I GCAGC 7 cut(s) 89, 193, 196, 199, 202, 359, 664
LweI GCATC 2 cut(s) 793, 1100
MaeI CTAG 2 cut(s) 63, 906
MaeII ACGT 4 cut(s) 80, 120, 840, 1052
MaeIII GTNAC 4 cut(s) 116, 121, 403, 781
MalI GATC 4 cut(s) 13, 476, 831, 1083
MboI GATC 4 cut(s) 11, 474, 829, 1081
MboII GAAGA 6 cut(s) 246, 533, 536, 574, 731, 953
MluCI AATT 4 cut(s) 533, 748, 1086, 1208
MluI ACGCGT 1 cut(s) 508
MlyI GAGTC 1 cut(s) 1064
MmeI TCCRAC 2 cut(s) 578, 1168
Mph1103I ATGCAT 1 cut(s) 506
MseI TTAA 4 cut(s) 362, 975, 1026, 1098
MslI CAYNNNNRTG 2 cut(s) 168, 499
MspA1I CMGCKG 1 cut(s) 219
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 3 cut(s) 265, 334, 605
MvaI CCWGG 3 cut(s) 265, 334, 605
MvnI CGCG 3 cut(s) 387, 418, 510
MwoI GCNNNNNNNGC 5 cut(s) 108, 187, 190, 384, 652
NdeI CATATG 1 cut(s) 231
NdeII GATC 4 cut(s) 11, 474, 829, 1081
NlaIII CATG 6 cut(s) 463, 718, 730, 794, 1003, 1078
NlaIV GGNNCC 4 cut(s) 240, 602, 997, 1164
NmuCI GTSAC 2 cut(s) 403, 781
NsiI ATGCAT 1 cut(s) 506
PauI GCGCGC 1 cut(s) 385
PfeI GAWTC 7 cut(s) 67, 550, 614, 730, 823, 1060, 1125
PfoI TCCNGGA 1 cut(s) 263
PkrI GCNGC 9 cut(s) 104, 183, 186, 189, 192, 374, 377, 654, 958
PleI GAGTC 1 cut(s) 1064
PpsI GAGTC 1 cut(s) 1064
PshBI ATTAAT 1 cut(s) 1098
Psp6I CCWGG 3 cut(s) 263, 332, 603
PspGI CCWGG 3 cut(s) 263, 332, 603
PspN4I GGNNCC 4 cut(s) 240, 602, 997, 1164
PspPI GGNCC 1 cut(s) 239
PstNI CAGNNNCTG 1 cut(s) 936
PteI GCGCGC 1 cut(s) 385
PvuII CAGCTG 1 cut(s) 219
RsaI GTAC 3 cut(s) 425, 513, 904
RsaNI GTAC 3 cut(s) 424, 512, 903
RseI CAYNNNNRTG 2 cut(s) 168, 499
SaqAI TTAA 4 cut(s) 362, 975, 1026, 1098
SatI GCNGC 9 cut(s) 103, 182, 185, 188, 191, 373, 376, 653, 957
Sau3AI GATC 4 cut(s) 11, 474, 829, 1081
Sau96I GGNCC 1 cut(s) 239
ScaI AGTACT 1 cut(s) 904
SchI GAGTC 1 cut(s) 1064
ScrFI CCNGG 3 cut(s) 265, 334, 605
SfaNI GCATC 2 cut(s) 793, 1100
SinI GGWCC 1 cut(s) 239
SmiMI CAYNNNNRTG 2 cut(s) 168, 499
SmlI CTYRAG 2 cut(s) 770, 1129
SmoI CTYRAG 2 cut(s) 770, 1129
SpeI ACTAGT 1 cut(s) 905
Sse9I AATT 4 cut(s) 533, 748, 1086, 1208
SsiI CCGC 4 cut(s) 375, 399, 608, 957
SspMI CTAG 2 cut(s) 63, 906
StyD4I CCNGG 3 cut(s) 263, 332, 603
TaaI ACNGT 2 cut(s) 288, 878
TaiI ACGT 4 cut(s) 83, 123, 843, 1055
TaqI TCGA 4 cut(s) 570, 687, 1068, 1191
TasI AATT 4 cut(s) 533, 748, 1086, 1208
TatI WGTACW 2 cut(s) 423, 902
TauI GCSGC 2 cut(s) 378, 959
TfiI GAWTC 7 cut(s) 67, 550, 614, 730, 823, 1060, 1125
Tru1I TTAA 4 cut(s) 362, 975, 1026, 1098
Tru9I TTAA 4 cut(s) 362, 975, 1026, 1098
TscAI CASTG 1 cut(s) 937
TseFI GTSAC 2 cut(s) 403, 781
TseI GCWGC 7 cut(s) 102, 181, 184, 187, 190, 372, 652
Tsp45I GTSAC 2 cut(s) 403, 781
TspDTI ATGAA 7 cut(s) 248, 476, 476, 703, 779, 911, 1004
TspRI CASTG 1 cut(s) 937
VpaK11BI GGWCC 1 cut(s) 239
VspI ATTAAT 1 cut(s) 1098
XapI RAATTY 2 cut(s) 1086, 1208
XmiI GTMKAC 2 cut(s) 843, 1197
XspI CTAG 2 cut(s) 63, 906
ZrmI AGTACT 1 cut(s) 904
Zsp2I ATGCAT 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.