MD10G1050900.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006934
Physical Location & Seq
Forward (+)
6724482 .. 6726008
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1050900.v1.1.491

Sequence Viewer

Length: 639 bp
ATGCGTGCACTTCTTTGCGGTTTGACATACAAGCACCTTAAATACAATCTCAAGGGCATTCATAATGATGTGATGAACATGGAAGACTTGTTGATCAAAACCTTGGGTTTTCCTGCCAACTGCATACGCATACTTTCGGAAGATGATCCGCTTGACCTAGAGCGGATTCCCACAAAGAAAAACATAGAGAATTCCTTGAAATGGCTTGTGGAGGACTGCCAGCGGGGAGACTCGTTGGTGTTCTACTTCTCAGGGCATGGCTTACGACAGTCTGATTTCAAAGACGATGAGCTTGATGGTTTCGACGAGACCATTTGCCCTGTTGATTTTATGAAAGAAGGAATTGTGCTTGACAATGATATTAATGAAACCATTGTTCGGCCACTCAAGGAGGGTGTTACACTCCTCGCTATCGTTGATGCTTCTAACATTAATGGAACGATTCTTGATCTGGAGTACGTGTACAACCATAAACTGAATGCTTGGAAAGAAAATATTCCTCCATCTGGTGTTAGAAAAAGTACGAATGGTGGGTTAGCGATTTCTCTCAGTACTTGCGAAGATAATACTACAGTAAGTGATGCTTCTATTCAATGTGATCAAAGTATGTTTAAGGAACATATAAAAGCCAACTTCTGA

Protein Analysis

213

Amino Acids

23.88

Weight (kDa)

4.81

Isoelectric Point (pI)

31.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 2 - 189 3.9e-23 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 163
AciI CCGC 4 cut(s) 18, 149, 163, 223
AclWI GGATC 1 cut(s) 140
AcoI YGGCCR 1 cut(s) 380
AcsI RAATTY 1 cut(s) 190
AfaI GTAC 4 cut(s) 458, 464, 523, 553
AfiI CCNNNNNNNGG 3 cut(s) 201, 378, 506
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 3 cut(s) 199, 280, 593
AluBI AGCT 1 cut(s) 292
AluI AGCT 1 cut(s) 292
Alw21I GWGCWC 1 cut(s) 10
Alw26I GTCTC 2 cut(s) 222, 302
Alw44I GTGCAC 1 cut(s) 6
AlwI GGATC 1 cut(s) 140
AoxI GGCC 1 cut(s) 380
ApaLI GTGCAC 1 cut(s) 6
ApoI RAATTY 1 cut(s) 190
AseI ATTAAT 2 cut(s) 363, 432
Asp700I GAANNNNTTC 1 cut(s) 495
BaeGI GKGCMC 1 cut(s) 10
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 10
BccI CCATC 2 cut(s) 290, 511
BclI TGATCA 2 cut(s) 93, 598
BcoDI GTCTC 2 cut(s) 222, 302
BfaI CTAG 1 cut(s) 158
BfmI CTRYAG 1 cut(s) 570
BmcAI AGTACT 1 cut(s) 553
BmsI GCATC 2 cut(s) 409, 571
BpiI GAAGAC 1 cut(s) 90
BpmI CTGGAG 1 cut(s) 473
BpuEI CTTGAG 2 cut(s) 35, 371
BsaAI YACGTR 1 cut(s) 460
BsaI GGTCTC 1 cut(s) 302
BsaJI CCNNGG 1 cut(s) 102
BsaXI ACNNNNNCTCC 2 cut(s) 446, 476
Bsc4I CCNNNNNNNGG 3 cut(s) 201, 378, 506
BseDI CCNNGG 1 cut(s) 102
BseLI CCNNNNNNNGG 3 cut(s) 201, 378, 506
BseMII CTCAG 2 cut(s) 264, 562
BseRI GAGGAG 1 cut(s) 395
BseSI GKGCMC 1 cut(s) 10
BshFI GGCC 1 cut(s) 382
BsiHKAI GWGCWC 1 cut(s) 10
BslI CCNNNNNNNGG 3 cut(s) 201, 378, 506
BsmAI GTCTC 2 cut(s) 222, 302
BsmI GAATGC 2 cut(s) 57, 484
BsnI GGCC 1 cut(s) 382
Bso31I GGTCTC 1 cut(s) 302
Bsp1286I GDGCHC 1 cut(s) 10
Bsp1407I TGTACA 1 cut(s) 462
Bsp143I GATC 4 cut(s) 93, 145, 448, 598
BspACI CCGC 4 cut(s) 18, 149, 163, 223
BspANI GGCC 1 cut(s) 382
BspCNI CTCAG 2 cut(s) 263, 561
BspPI GGATC 1 cut(s) 140
BspTNI GGTCTC 1 cut(s) 302
BsrBI CCGCTC 1 cut(s) 163
BsrGI TGTACA 1 cut(s) 462
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 4 cut(s) 93, 145, 448, 598
BssT1I CCWWGG 1 cut(s) 102
Bst4CI ACNGT 2 cut(s) 270, 574
BstAUI TGTACA 1 cut(s) 462
BstBAI YACGTR 1 cut(s) 460
BstC8I GCNNGC 2 cut(s) 6, 221
BstDEI CTNAG 2 cut(s) 250, 548
BstKTI GATC 4 cut(s) 96, 148, 451, 601
BstMAI GTCTC 2 cut(s) 222, 302
BstMBI GATC 4 cut(s) 93, 145, 448, 598
BstSFI CTRYAG 1 cut(s) 570
BstSLI GKGCMC 1 cut(s) 10
BstV2I GAAGAC 1 cut(s) 90
BsuRI GGCC 1 cut(s) 382
Cac8I GCNNGC 2 cut(s) 6, 221
Csp6I GTAC 4 cut(s) 457, 463, 522, 552
CviAII CATG 2 cut(s) 79, 257
CviJI RGCY 5 cut(s) 205, 261, 292, 382, 629
CviKI_1 RGCY 5 cut(s) 205, 261, 292, 382, 629
CviQI GTAC 4 cut(s) 457, 463, 522, 552
DdeI CTNAG 2 cut(s) 250, 548
DpnI GATC 4 cut(s) 95, 147, 450, 600
DpnII GATC 4 cut(s) 93, 145, 448, 598
EaeI YGGCCR 1 cut(s) 380
Eco130I CCWWGG 1 cut(s) 102
Eco31I GGTCTC 1 cut(s) 302
EcoRI GAATTC 1 cut(s) 190
EcoT14I CCWWGG 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 2 cut(s) 82, 260
FalI AAGNNNNNCTT 2 cut(s) 568, 600
FatI CATG 2 cut(s) 78, 256
FauI CCCGC 1 cut(s) 216
FbaI TGATCA 2 cut(s) 93, 598
FspBI CTAG 1 cut(s) 158
GsuI CTGGAG 1 cut(s) 473
HaeIII GGCC 1 cut(s) 382
Hin1II CATG 2 cut(s) 82, 260
HinfI GANTC 3 cut(s) 166, 230, 442
Hpy166II GTNNAC 2 cut(s) 8, 463
Hpy188I TCNGA 3 cut(s) 139, 274, 638
Hpy188III TCNNGA 2 cut(s) 446, 452
Hpy8I GTNNAC 2 cut(s) 8, 463
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 1 cut(s) 332
HpyCH4III ACNGT 2 cut(s) 270, 574
HpyCH4IV ACGT 1 cut(s) 459
HpyCH4V TGCA 2 cut(s) 8, 123
HpyF3I CTNAG 2 cut(s) 250, 548
HpySE526I ACGT 1 cut(s) 459
Hsp92II CATG 2 cut(s) 82, 260
Ksp22I TGATCA 2 cut(s) 93, 598
Kzo9I GATC 4 cut(s) 93, 145, 448, 598
LpnPI CCDG 6 cut(s) 126, 233, 237, 333, 437, 492
LweI GCATC 2 cut(s) 409, 571
MaeI CTAG 1 cut(s) 158
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 1 cut(s) 397
MalI GATC 4 cut(s) 95, 147, 450, 600
MbiI CCGCTC 1 cut(s) 163
MboI GATC 4 cut(s) 93, 145, 448, 598
MboII GAAGA 3 cut(s) 95, 152, 572
MhlI GDGCHC 1 cut(s) 10
MluCI AATT 2 cut(s) 190, 342
MlyI GAGTC 1 cut(s) 224
MnlI CCTC 4 cut(s) 205, 385, 416, 510
MroXI GAANNNNTTC 1 cut(s) 495
MseI TTAA 4 cut(s) 39, 363, 432, 612
MslI CAYNNNNRTG 1 cut(s) 66
MspA1I CMGCKG 1 cut(s) 223
Mva1269I GAATGC 2 cut(s) 57, 484
NdeII GATC 4 cut(s) 93, 145, 448, 598
NlaIII CATG 2 cut(s) 82, 260
PctI GAATGC 2 cut(s) 57, 484
PdmI GAANNNNTTC 1 cut(s) 495
PfeI GAWTC 2 cut(s) 166, 442
PleI GAGTC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 224
Ppu21I YACGTR 1 cut(s) 460
PshBI ATTAAT 2 cut(s) 363, 432
RsaI GTAC 4 cut(s) 458, 464, 523, 553
RsaNI GTAC 4 cut(s) 457, 463, 522, 552
RseI CAYNNNNRTG 1 cut(s) 66
SaqAI TTAA 4 cut(s) 39, 363, 432, 612
Sau3AI GATC 4 cut(s) 93, 145, 448, 598
ScaI AGTACT 1 cut(s) 553
SchI GAGTC 1 cut(s) 224
SduI GDGCHC 1 cut(s) 10
SetI ASST 5 cut(s) 39, 104, 159, 294, 462
SfaNI GCATC 2 cut(s) 409, 571
SfcI CTRYAG 1 cut(s) 570
SmiMI CAYNNNNRTG 1 cut(s) 66
SmlI CTYRAG 2 cut(s) 50, 386
SmoI CTYRAG 2 cut(s) 50, 386
Sse9I AATT 2 cut(s) 190, 342
SsiI CCGC 4 cut(s) 18, 149, 163, 223
SspI AATATT 1 cut(s) 496
SspMI CTAG 1 cut(s) 158
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 2 cut(s) 270, 574
TaiI ACGT 1 cut(s) 462
TaqI TCGA 1 cut(s) 303
TasI AATT 2 cut(s) 190, 342
TatI WGTACW 2 cut(s) 462, 551
TfiI GAWTC 2 cut(s) 166, 442
Tru1I TTAA 4 cut(s) 39, 363, 432, 612
Tru9I TTAA 4 cut(s) 39, 363, 432, 612
TspDTI ATGAA 4 cut(s) 50, 89, 347, 381
VneI GTGCAC 1 cut(s) 6
VspI ATTAAT 2 cut(s) 363, 432
XapI RAATTY 1 cut(s) 190
XmnI GAANNNNTTC 1 cut(s) 495
XspI CTAG 1 cut(s) 158
ZrmI AGTACT 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.