MD05G1043500.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006934
Physical Location & Seq
Forward (+)
7386084 .. 7389247
3164 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1043500.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGACGCTGTATGGCACACAGCACCGATGCGTCTGCGGTCATGTAATCACGACTCGCAGTGCTGCTGCAGAAACACAACGAGAAACTAGTATTCATGGTCATGGAGATCACAAGCGTGAGCGTCATAGCTTGCGGAGATGGCTTGCCAGGTGTTTTCAAACCAAGGAAGCATCATCAAACAATTCTTCTAGTAGTTGTAGTTCAAGTCGTAATAGTTTCAAGCAATCTTATACAGGGACGGGGACGCTGATGTCGTTGCCCTCCTCCCGCTCGGAGCAGCCAGTACCCAGTGGATCACGACCAAACAAGCGTGCACTTCTTTGCGGGGTGACATACAAGCAACATAAATACAAGCTCAAAGGCACTTATAATGATGTAAAAAACATGGAAAACTTGCTGACTAAGACCTTCGGTTTTCCTGCCAACTGCATACGCATACTTTCAGAAGATGATCCGCAAGACCTAGAGCGGATTCCTACAAAGAAAAATATAGAAAATTCCTTGAAATGGCTTGTGGAGGGATGCCAGCGGGGAGACTCGTTGGTGTTTTACTTCTCAGGGCATGGCTTACGACAGCCTGATTTCAAAGACGATGAGCTTGATGGGTTCGACGAGACCATTTGCCCTGTTGATTTTATGAAAGAGGGAATGATCCTTGACAATGATATTAATGAAACCATTGTTTGGCCACTCGAGGAGGGTGTCACACTTCACGCTATTGTTGATGCTTGTCACAGCGGAACGATTCTTGATCTGGAGCACGTGTATGACCGTAAACTGAATATTTGGAAAGATAATAGTCCTCCATCTCGTGCTAGAAAAAGTACAAGGGGTGGGTTAGCGATTTCTCTTAGTGCTTGCGAAGATGATAAAATGGCAGCTGATACTACTGCTTTTGCTGGAAAATCAATGAATGGTGCAATGACATACATTCTCACTGAAACTGTTAGGAAGAAGACCGACATAACATATGGAATTTTGCTCGACGAGATGGTAAATTACATTAAAAAGATTAACAGCGAAAGATGGACTAACTCAAGATTCCTGAGGACTGTACTTCGACACAAAACCATACAGGAACCTATGTTGTCATCTTCTGAAACGTTTGATGTTAATTTGAAGAAATTTGTCTTGTAA

Protein Analysis

379

Amino Acids

42.76

Weight (kDa)

8.57

Isoelectric Point (pI)

53.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 103 - 366 2e-31 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 369
AasI GACNNNNNNGTC 1 cut(s) 250
AccB7I CCANNNNNTGG 1 cut(s) 684
AccBSI CCGCTC 2 cut(s) 270, 469
AciI CCGC 8 cut(s) 36, 133, 268, 324, 455, 469, 529, 738
AclI AACGTT 1 cut(s) 1103
AclWI GGATC 3 cut(s) 301, 446, 646
AcoI YGGCCR 1 cut(s) 686
AcsI RAATTY 3 cut(s) 496, 975, 1124
AcvI CACGTG 1 cut(s) 763
AfaI GTAC 3 cut(s) 285, 826, 1056
AfiI CCNNNNNNNGG 2 cut(s) 507, 684
AflIII ACRYGT 1 cut(s) 762
AgsI TTSAA 6 cut(s) 158, 204, 220, 505, 586, 1120
AhlI ACTAGT 1 cut(s) 86
AjnI CCWGG 1 cut(s) 146
AleI CACNNNNGTG 1 cut(s) 114
AluBI AGCT 4 cut(s) 129, 355, 598, 881
AluI AGCT 4 cut(s) 129, 355, 598, 881
Alw21I GWGCWC 2 cut(s) 316, 762
Alw26I GTCTC 2 cut(s) 528, 608
Alw44I GTGCAC 1 cut(s) 312
AlwI GGATC 3 cut(s) 301, 446, 646
Ama87I CYCGRG 1 cut(s) 692
AoxI GGCC 1 cut(s) 686
ApaLI GTGCAC 1 cut(s) 312
ApeKI GCWGC 4 cut(s) 62, 65, 277, 878
ApoI RAATTY 3 cut(s) 496, 975, 1124
AseI ATTAAT 1 cut(s) 669
AsuHPI GGTGA 1 cut(s) 340
AvaI CYCGRG 1 cut(s) 692
AxyI CCTNAGG 1 cut(s) 1046
BaeGI GKGCMC 1 cut(s) 316
BalI TGGCCA 1 cut(s) 688
BauI CACGAG 1 cut(s) 810
BbrPI CACGTG 1 cut(s) 763
BbsI GAAGAC 1 cut(s) 962
Bbv12I GWGCWC 2 cut(s) 316, 762
BbvI GCAGC 4 cut(s) 49, 52, 289, 890
BccI CCATC 5 cut(s) 132, 596, 814, 985, 1020
BcgI CGANNNNNNTGC 2 cut(s) 15, 49
BciT130I CCWGG 1 cut(s) 148
BcoDI GTCTC 2 cut(s) 528, 608
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 4 cut(s) 87, 189, 464, 816
BfmI CTRYAG 1 cut(s) 66
BisI GCNGC 4 cut(s) 63, 66, 278, 879
BlsI GCNGC 4 cut(s) 64, 67, 279, 880
Bme1390I CCNGG 1 cut(s) 148
BmeT110I CYCGRG 1 cut(s) 692
BmiI GGNNCC 1 cut(s) 1080
BmrFI CCNGG 1 cut(s) 148
BmrI ACTGGG 1 cut(s) 282
BmsI GCATC 4 cut(s) 17, 179, 512, 715
BmuI ACTGGG 1 cut(s) 282
BpiI GAAGAC 1 cut(s) 962
BpmI CTGGAG 1 cut(s) 776
BpuEI CTTGAG 1 cut(s) 1021
BsaAI YACGTR 1 cut(s) 763
BsaI GGTCTC 1 cut(s) 608
BsaJI CCNNGG 1 cut(s) 162
BsaXI ACNNNNNCTCC 2 cut(s) 749, 779
Bsc4I CCNNNNNNNGG 2 cut(s) 507, 684
Bse1I ACTGG 2 cut(s) 281, 288
Bse21I CCTNAGG 1 cut(s) 1046
Bse3DI GCAATG 1 cut(s) 927
BseBI CCWGG 1 cut(s) 148
BseDI CCNNGG 1 cut(s) 162
BseGI GGATG 1 cut(s) 527
BseLI CCNNNNNNNGG 2 cut(s) 507, 684
BseMI GCAATG 1 cut(s) 927
BseMII CTCAG 2 cut(s) 570, 1037
BseNI ACTGG 2 cut(s) 281, 288
BseRI GAGGAG 2 cut(s) 253, 710
BseSI GKGCMC 1 cut(s) 316
BseXI GCAGC 4 cut(s) 49, 52, 289, 890
BshFI GGCC 1 cut(s) 688
BsiHKAI GWGCWC 2 cut(s) 316, 762
BsiHKCI CYCGRG 1 cut(s) 692
BslFI GGGAC 2 cut(s) 250, 256
BslI CCNNNNNNNGG 2 cut(s) 507, 684
BsmAI GTCTC 2 cut(s) 528, 608
BsmFI GGGAC 2 cut(s) 250, 256
BsnI GGCC 1 cut(s) 688
Bso31I GGTCTC 1 cut(s) 608
BsoBI CYCGRG 1 cut(s) 692
Bsp1286I GDGCHC 2 cut(s) 316, 762
Bsp143I GATC 5 cut(s) 106, 293, 451, 651, 751
BspACI CCGC 8 cut(s) 36, 133, 268, 324, 455, 469, 529, 738
BspANI GGCC 1 cut(s) 688
BspCNI CTCAG 2 cut(s) 569, 1038
BspLI GGNNCC 1 cut(s) 1080
BspMAI CTGCAG 1 cut(s) 70
BspPI GGATC 3 cut(s) 301, 446, 646
BspTNI GGTCTC 1 cut(s) 608
BsrBI CCGCTC 2 cut(s) 270, 469
BsrDI GCAATG 1 cut(s) 927
BsrI ACTGG 2 cut(s) 281, 288
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 5 cut(s) 106, 293, 451, 651, 751
BssSI CACGAG 1 cut(s) 810
BssT1I CCWWGG 1 cut(s) 162
Bst2BI CACGAG 1 cut(s) 810
Bst2UI CCWGG 1 cut(s) 148
Bst4CI ACNGT 3 cut(s) 773, 946, 1054
BstBAI YACGTR 1 cut(s) 763
BstC8I GCNNGC 5 cut(s) 131, 144, 312, 527, 859
BstDEI CTNAG 4 cut(s) 402, 556, 851, 1046
BstF5I GGATG 1 cut(s) 527
BstKTI GATC 5 cut(s) 109, 296, 454, 654, 754
BstMAI GTCTC 2 cut(s) 528, 608
BstMBI GATC 5 cut(s) 106, 293, 451, 651, 751
BstMWI GCNNNNNNNGC 1 cut(s) 139
BstNI CCWGG 1 cut(s) 148
BstSCI CCNGG 1 cut(s) 146
BstSFI CTRYAG 1 cut(s) 66
BstSLI GKGCMC 1 cut(s) 316
BstV1I GCAGC 4 cut(s) 49, 52, 289, 890
BstV2I GAAGAC 1 cut(s) 962
Bsu36I CCTNAGG 1 cut(s) 1046
BsuRI GGCC 1 cut(s) 688
BtsCI GGATG 1 cut(s) 527
BtsI GCAGTG 1 cut(s) 64
BtsIMutI CAGTG 3 cut(s) 64, 295, 936
Cac8I GCNNGC 5 cut(s) 131, 144, 312, 527, 859
CseI GACGC 4 cut(s) 13, 19, 110, 253
Csp6I GTAC 3 cut(s) 284, 825, 1055
CviAII CATG 5 cut(s) 41, 95, 101, 385, 563
CviQI GTAC 3 cut(s) 284, 825, 1055
DdeI CTNAG 4 cut(s) 402, 556, 851, 1046
DpnI GATC 5 cut(s) 108, 295, 453, 653, 753
DpnII GATC 5 cut(s) 106, 293, 451, 651, 751
DrdI GACNNNNNNGTC 1 cut(s) 250
DseDI GACNNNNNNGTC 1 cut(s) 250
EaeI YGGCCR 1 cut(s) 686
Eco130I CCWWGG 1 cut(s) 162
Eco31I GGTCTC 1 cut(s) 608
Eco72I CACGTG 1 cut(s) 763
Eco81I CCTNAGG 1 cut(s) 1046
Eco88I CYCGRG 1 cut(s) 692
EcoRII CCWGG 1 cut(s) 146
EcoT14I CCWWGG 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 5 cut(s) 44, 98, 104, 388, 566
FaqI GGGAC 2 cut(s) 250, 256
FatI CATG 5 cut(s) 40, 94, 100, 384, 562
FauI CCCGC 3 cut(s) 275, 317, 522
FauNDI CATATG 1 cut(s) 970
Fnu4HI GCNGC 4 cut(s) 63, 66, 278, 879
FokI GGATG 1 cut(s) 534
Fsp4HI GCNGC 4 cut(s) 63, 66, 278, 879
FspBI CTAG 4 cut(s) 87, 189, 464, 816
GluI GCNGC 4 cut(s) 63, 66, 278, 879
GsuI CTGGAG 1 cut(s) 776
HaeIII GGCC 1 cut(s) 688
HgaI GACGC 4 cut(s) 13, 19, 110, 253
Hin1II CATG 5 cut(s) 44, 98, 104, 388, 566
HinfI GANTC 5 cut(s) 52, 472, 536, 745, 1041
HphI GGTGA 1 cut(s) 340
Hpy166II GTNNAC 2 cut(s) 314, 776
Hpy188I TCNGA 3 cut(s) 274, 445, 1099
Hpy188III TCNNGA 6 cut(s) 49, 297, 749, 755, 1038, 1045
Hpy8I GTNNAC 2 cut(s) 314, 776
Hpy99I CGWCG 2 cut(s) 614, 989
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 3 cut(s) 773, 946, 1054
HpyCH4IV ACGT 2 cut(s) 762, 1103
HpyCH4V TGCA 4 cut(s) 68, 314, 429, 920
HpyF10VI GCNNNNNNNGC 1 cut(s) 139
HpyF3I CTNAG 4 cut(s) 402, 556, 851, 1046
HpySE526I ACGT 2 cut(s) 762, 1103
Hsp92II CATG 5 cut(s) 44, 98, 104, 388, 566
Kzo9I GATC 5 cut(s) 106, 293, 451, 651, 751
LmnI GCTCC 2 cut(s) 274, 757
Lsp1109I GCAGC 4 cut(s) 49, 52, 289, 890
LweI GCATC 4 cut(s) 17, 179, 512, 715
MaeI CTAG 4 cut(s) 87, 189, 464, 816
MaeII ACGT 2 cut(s) 762, 1103
MaeIII GTNAC 3 cut(s) 328, 703, 731
MalI GATC 5 cut(s) 108, 295, 453, 653, 753
MbiI CCGCTC 2 cut(s) 270, 469
MboI GATC 5 cut(s) 106, 293, 451, 651, 751
MboII GAAGA 7 cut(s) 177, 458, 875, 964, 967, 1086, 1132
MhlI GDGCHC 2 cut(s) 316, 762
MlsI TGGCCA 1 cut(s) 688
MluCI AATT 6 cut(s) 181, 496, 975, 997, 1114, 1124
MluNI TGGCCA 1 cut(s) 688
MlyI GAGTC 2 cut(s) 46, 530
MnlI CCTC 8 cut(s) 271, 274, 511, 637, 688, 691, 813, 1041
Mox20I TGGCCA 1 cut(s) 688
MscI TGGCCA 1 cut(s) 688
MseI TTAA 4 cut(s) 669, 1005, 1014, 1113
MslI CAYNNNNRTG 3 cut(s) 99, 114, 765
Msp20I TGGCCA 1 cut(s) 688
MspA1I CMGCKG 3 cut(s) 529, 738, 881
MspR9I CCNGG 1 cut(s) 148
MvaI CCWGG 1 cut(s) 148
MwoI GCNNNNNNNGC 1 cut(s) 139
NdeI CATATG 1 cut(s) 970
NdeII GATC 5 cut(s) 106, 293, 451, 651, 751
NlaIII CATG 5 cut(s) 44, 98, 104, 388, 566
NlaIV GGNNCC 1 cut(s) 1080
NmuCI GTSAC 3 cut(s) 328, 703, 731
OliI CACNNNNGTG 1 cut(s) 114
PaeR7I CTCGAG 1 cut(s) 692
PcsI WCGNNNNNNNCGW 1 cut(s) 251
PfeI GAWTC 3 cut(s) 472, 745, 1041
PflMI CCANNNNNTGG 1 cut(s) 684
PkrI GCNGC 4 cut(s) 64, 67, 279, 880
PleI GAGTC 2 cut(s) 46, 530
PmaCI CACGTG 1 cut(s) 763
PmlI CACGTG 1 cut(s) 763
PpsI GAGTC 2 cut(s) 46, 530
Ppu21I YACGTR 1 cut(s) 763
PshBI ATTAAT 1 cut(s) 669
PsiI TTATAA 1 cut(s) 369
Psp1406I AACGTT 1 cut(s) 1103
Psp6I CCWGG 1 cut(s) 146
PspCI CACGTG 1 cut(s) 763
PspGI CCWGG 1 cut(s) 146
PspN4I GGNNCC 1 cut(s) 1080
PspXI VCTCGAGB 1 cut(s) 692
PsrI GAACNNNNNNTAC 2 cut(s) 184, 216
PstI CTGCAG 1 cut(s) 70
PvuII CAGCTG 1 cut(s) 881
RsaI GTAC 3 cut(s) 285, 826, 1056
RsaNI GTAC 3 cut(s) 284, 825, 1055
RseI CAYNNNNRTG 3 cut(s) 99, 114, 765
SaqAI TTAA 4 cut(s) 669, 1005, 1014, 1113
SatI GCNGC 4 cut(s) 63, 66, 278, 879
Sau3AI GATC 5 cut(s) 106, 293, 451, 651, 751
SchI GAGTC 2 cut(s) 46, 530
ScrFI CCNGG 1 cut(s) 148
SduI GDGCHC 2 cut(s) 316, 762
SfaNI GCATC 4 cut(s) 17, 179, 512, 715
SfcI CTRYAG 1 cut(s) 66
Sfr274I CTCGAG 1 cut(s) 692
SlaI CTCGAG 1 cut(s) 692
SmiMI CAYNNNNRTG 3 cut(s) 99, 114, 765
SmlI CTYRAG 2 cut(s) 692, 1036
SmoI CTYRAG 2 cut(s) 692, 1036
SpeI ACTAGT 1 cut(s) 86
Sse9I AATT 6 cut(s) 181, 496, 975, 997, 1114, 1124
SsiI CCGC 8 cut(s) 36, 133, 268, 324, 455, 469, 529, 738
SspI AATATT 1 cut(s) 784
SspMI CTAG 4 cut(s) 87, 189, 464, 816
StyD4I CCNGG 1 cut(s) 146
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 3 cut(s) 773, 946, 1054
TaiI ACGT 2 cut(s) 765, 1106
TaqI TCGA 4 cut(s) 609, 693, 984, 1060
TaqII GACCGA 1 cut(s) 974
TasI AATT 6 cut(s) 181, 496, 975, 997, 1114, 1124
TatI WGTACW 2 cut(s) 824, 1054
TfiI GAWTC 3 cut(s) 472, 745, 1041
Tru1I TTAA 4 cut(s) 669, 1005, 1014, 1113
Tru9I TTAA 4 cut(s) 669, 1005, 1014, 1113
TscAI CASTG 3 cut(s) 64, 295, 943
TseFI GTSAC 3 cut(s) 328, 703, 731
TseI GCWGC 4 cut(s) 62, 65, 277, 878
Tsp45I GTSAC 3 cut(s) 328, 703, 731
TspDTI ATGAA 4 cut(s) 83, 653, 687, 926
TspRI CASTG 3 cut(s) 64, 295, 943
Van91I CCANNNNNTGG 1 cut(s) 684
VneI GTGCAC 1 cut(s) 312
VspI ATTAAT 1 cut(s) 669
XapI RAATTY 3 cut(s) 496, 975, 1124
XhoI CTCGAG 1 cut(s) 692
XspI CTAG 4 cut(s) 87, 189, 464, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.