FvH4_5g28770

Cleavage and polyadenylation specificity factor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002312
Physical Location & Seq
Reverse (-)
19917412 .. 19919123
1712 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g28770.t1

Sequence Viewer

Length: 1311 bp
ATGACATATGCAACCAAGGCTATCTATAAGTTGCTTTTGACAGATTATGTGAAAGTCAGCAAAGTTTCTGTTGAAGATATGTTGTTCGACGAGCAGGACATTAATGCTTCTATGGATAAAATTGAGGTTATTGATTTCCACCAAACAGTAGAAGTTGAGGGCATTCGGTTTTGGTGCTACACTGCAGGTCATGTCCTTGGTGCTGCTATGTTTATGGTTGATATTGCCGGTGTTAGGGTCCTCTATACTGGAGATTATTCACGCGAAGAAGATCGACACCTCCGTGCTGCTGAGACTCCACAGTTCTCGCCTGATATATGCATTATTGAATCCACATATGGTGTCCAGCACCATCAACCTCGAAACATCCGTGAGAAGCGATTCACTGAAGCTATACACACTGCTCTCTCTCGAGGGGGCCGTGTCTTAATTCCTGCATTTGCACTTGGCCGTGCCCAAGAACTTCTCCTGATCCTTGATGAGTATTGGGCAAATCATCCAGAGCTTCAAAATATTCCTATATACTATTCTTCCCCTCTGGCAAAAAGATGTTTATCTGTTTATGAGACATACACCCTCTCAATGAAAGATAGGATCCGCAATGCGAAGTCAAATCCCTTCCTCTTTAAGTACATATCACCACTAAAGAGCATTGAGAATTTCAAAGATGTAGGCCCCTCGGTCGTGATGGCAAGCCCTGGTGGTCTTCAAAGCGGGTTGTCAAGGCAGCTTTTTGATATGTGGTGCTCTGATAAGAGAAATGCATGTGTGATTCCAGGTTTTGTGGTTGAGGGGACACTTGCAAAGACAATCATCAATGAACCGAAAGAGGTCACCCTTATGAATGGGCTAGCTGCTCCGCTCAACATGTCGGTACATTACATTTCATTCTCTGCTCATGCAGACTCTGTTCAGACAAGTGCATTTTTGGAGGAGCTGAGGCCTCCTAACATAATCCTTGTTCATGGAGAAGCTCATGAGATGGGAAGGCTGAAAGAGAAGCTCATGACTCAGTTTCGTGATCGCAACACCAAAATCCTCACACCAAAGAATTGTCAGTCTGTTGAGATGTATTTCAACTTGCAGAAAATGGCTAAAGCTATTGGAAGGGTTGCTGAGAAGACCCCGGAAGTTGGAGAAAAAGTAAGCGGTTTACTGGTAAAAAAAGGTTTCAGTTATCAGATTATGGCTTCCGAAGATCTTCATGTTTTCTCGCAGCTGTCAACGGCAAATGTCACGCAGGGTATTACTATCCCGTATACTACGTACTGGCTTTATGGTGATAAAACATCGGTTGAGGCAAATATATGA

Protein Analysis

437

Amino Acids

49.2

Weight (kDa)

6.65

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B_6 PF16661 3 - 99 1.4e-10 Metallo-beta-lactamase superfamily domain
Beta-Casp PF10996 152 - 271 3.9e-28 Beta-Casp domain
RMMBL PF07521 287 - 339 4e-13 Zn-dependent metallo-hydrolase RNA specificity domain
CPSF73-100_C PF11718 381 - 422 2.9e-11 Pre-mRNA 3'-end-processing endonuclease polyadenylation factor C-term
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 176
AccBSI CCGCTC 1 cut(s) 862
AccI GTMKAC 1 cut(s) 1259
AccII CGCG 1 cut(s) 264
AciI CCGC 4 cut(s) 598, 714, 860, 1149
AclWI GGATC 3 cut(s) 466, 589, 602
AcoI YGGCCR 1 cut(s) 448
AcsI RAATTY 1 cut(s) 658
AcuI CTGAAG 1 cut(s) 408
AfaI GTAC 3 cut(s) 632, 876, 1268
AfiI CCNNNNNNNGG 2 cut(s) 234, 1133
AflIII ACRYGT 1 cut(s) 867
AgsI TTSAA 6 cut(s) 74, 329, 509, 664, 710, 1078
AjnI CCWGG 2 cut(s) 697, 775
AleI CACNNNNGTG 1 cut(s) 282
AluBI AGCT 9 cut(s) 392, 505, 730, 854, 937, 974, 1003, 1100, 1219
AluI AGCT 9 cut(s) 392, 505, 730, 854, 937, 974, 1003, 1100, 1219
Alw21I GWGCWC 1 cut(s) 749
Alw26I GTCTC 2 cut(s) 287, 560
AlwI GGATC 3 cut(s) 466, 589, 602
AlwNI CAGNNNCTG 1 cut(s) 908
Ama87I CYCGRG 1 cut(s) 411
AoxI GGCC 4 cut(s) 418, 448, 673, 941
ApeKI GCWGC 5 cut(s) 203, 287, 727, 854, 1216
ApoI RAATTY 1 cut(s) 658
AseI ATTAAT 1 cut(s) 102
Asp700I GAANNNNTTC 2 cut(s) 380, 1200
AspS9I GGNCC 3 cut(s) 238, 418, 674
AsuC2I CCSGG 1 cut(s) 1127
AsuHPI GGTGA 3 cut(s) 630, 826, 1292
AsuNHI GCTAGC 1 cut(s) 850
AvaI CYCGRG 1 cut(s) 411
AvaII GGWCC 1 cut(s) 238
BaeGI GKGCMC 1 cut(s) 457
BamHI GGATCC 1 cut(s) 594
BbsI GAAGAC 2 cut(s) 698, 1127
Bbv12I GWGCWC 1 cut(s) 749
BbvCI CCTCAGC 1 cut(s) 938
BbvI GCAGC 5 cut(s) 190, 274, 739, 841, 1228
BccI CCATC 3 cut(s) 360, 682, 976
BceAI ACGGC 3 cut(s) 405, 435, 1242
BciT130I CCWGG 2 cut(s) 699, 777
BcnI CCSGG 1 cut(s) 1127
BcoDI GTCTC 2 cut(s) 287, 560
BfaI CTAG 1 cut(s) 851
BfmI CTRYAG 1 cut(s) 183
BfuAI ACCTGC 1 cut(s) 176
BglII AGATCT 1 cut(s) 1198
BisI GCNGC 5 cut(s) 204, 288, 728, 855, 1217
BlsI GCNGC 5 cut(s) 205, 289, 729, 856, 1218
Bme1390I CCNGG 3 cut(s) 699, 777, 1127
Bme18I GGWCC 1 cut(s) 238
BmeT110I CYCGRG 1 cut(s) 411
BmgT120I GGNCC 3 cut(s) 238, 418, 674
BmiI GGNNCC 4 cut(s) 239, 419, 596, 676
BmrFI CCNGG 3 cut(s) 699, 777, 1127
BmtI GCTAGC 1 cut(s) 854
BpiI GAAGAC 2 cut(s) 698, 1127
BpmI CTGGAG 1 cut(s) 270
Bpu10I CCTNAGC 1 cut(s) 938
BpuMI CCSGG 1 cut(s) 1127
BsaAI YACGTR 1 cut(s) 1266
BsaBI GATNNNNATC 1 cut(s) 553
BsaJI CCNNGG 5 cut(s) 15, 196, 678, 697, 1125
BsaXI ACNNNNNCTCC 2 cut(s) 1128, 1158
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 1133
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 3 cut(s) 253, 1161, 1274
Bse3DI GCAATG 1 cut(s) 607
Bse8I GATNNNNATC 1 cut(s) 553
BseBI CCWGG 2 cut(s) 699, 777
BseDI CCNNGG 5 cut(s) 15, 196, 678, 697, 1125
BseGI GGATG 2 cut(s) 366, 496
BseJI GATNNNNATC 1 cut(s) 553
BseLI CCNNNNNNNGG 2 cut(s) 234, 1133
BseMI GCAATG 1 cut(s) 607
BseMII CTCAG 4 cut(s) 282, 929, 1025, 1107
BseNI ACTGG 3 cut(s) 253, 1161, 1274
BseRI GAGGAG 1 cut(s) 947
BseSI GKGCMC 1 cut(s) 457
BseXI GCAGC 5 cut(s) 190, 274, 739, 841, 1228
Bsh1236I CGCG 1 cut(s) 264
Bsh1285I CGRYCG 1 cut(s) 684
BshFI GGCC 4 cut(s) 420, 450, 675, 943
BsiEI CGRYCG 1 cut(s) 684
BsiHKAI GWGCWC 1 cut(s) 749
BsiHKCI CYCGRG 1 cut(s) 411
BsiSI CCGG 2 cut(s) 228, 1127
BslFI GGGAC 1 cut(s) 808
BslI CCNNNNNNNGG 2 cut(s) 234, 1133
BsmAI GTCTC 2 cut(s) 287, 560
BsmFI GGGAC 1 cut(s) 808
BsmI GAATGC 1 cut(s) 162
BsnI GGCC 4 cut(s) 420, 450, 675, 943
BsoBI CYCGRG 1 cut(s) 411
Bsp1286I GDGCHC 2 cut(s) 457, 749
Bsp143I GATC 5 cut(s) 271, 471, 594, 1021, 1198
BspACI CCGC 4 cut(s) 598, 714, 860, 1149
BspANI GGCC 4 cut(s) 420, 450, 675, 943
BspCNI CTCAG 4 cut(s) 283, 930, 1024, 1108
BspFNI CGCG 1 cut(s) 264
BspHI TCATGA 2 cut(s) 976, 1005
BspLI GGNNCC 4 cut(s) 239, 419, 596, 676
BspMAI CTGCAG 1 cut(s) 187
BspMI ACCTGC 1 cut(s) 176
BspOI GCTAGC 1 cut(s) 854
BspPI GGATC 3 cut(s) 466, 589, 602
BsrBI CCGCTC 1 cut(s) 862
BsrDI GCAATG 1 cut(s) 607
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 3 cut(s) 253, 1161, 1274
BssAI RCCGGY 1 cut(s) 227
BssECI CCNNGG 5 cut(s) 15, 196, 678, 697, 1125
BssMI GATC 5 cut(s) 271, 471, 594, 1021, 1198
BssNAI GTATAC 1 cut(s) 1260
BssT1I CCWWGG 2 cut(s) 15, 196
Bst1107I GTATAC 1 cut(s) 1260
Bst2UI CCWGG 2 cut(s) 699, 777
Bst4CI ACNGT 2 cut(s) 148, 303
BstBAI YACGTR 1 cut(s) 1266
BstC8I GCNNGC 2 cut(s) 694, 852
BstDEI CTNAG 4 cut(s) 291, 938, 1011, 1116
BstEII GGTNACC 1 cut(s) 832
BstF5I GGATG 2 cut(s) 366, 496
BstFNI CGCG 1 cut(s) 264
BstKTI GATC 5 cut(s) 274, 474, 597, 1024, 1201
BstMAI GTCTC 2 cut(s) 287, 560
BstMBI GATC 5 cut(s) 271, 471, 594, 1021, 1198
BstMCI CGRYCG 1 cut(s) 684
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNI CCWGG 2 cut(s) 699, 777
BstNSI RCATGY 2 cut(s) 768, 871
BstPI GGTNACC 1 cut(s) 832
BstSCI CCNGG 3 cut(s) 697, 775, 1125
BstSFI CTRYAG 1 cut(s) 183
BstSLI GKGCMC 1 cut(s) 457
BstSNI TACGTA 1 cut(s) 1266
BstUI CGCG 1 cut(s) 264
BstV1I GCAGC 5 cut(s) 190, 274, 739, 841, 1228
BstV2I GAAGAC 2 cut(s) 698, 1127
BstX2I RGATCY 2 cut(s) 594, 1198
BstYI RGATCY 2 cut(s) 594, 1198
BstZ17I GTATAC 1 cut(s) 1260
BsuRI GGCC 4 cut(s) 420, 450, 675, 943
BtsCI GGATG 2 cut(s) 366, 496
BtsI GCAGTG 2 cut(s) 180, 399
BtsIMutI CAGTG 3 cut(s) 180, 384, 399
BveI ACCTGC 1 cut(s) 176
Cac8I GCNNGC 2 cut(s) 694, 852
CaiI CAGNNNCTG 1 cut(s) 908
CciI TCATGA 2 cut(s) 976, 1005
Cfr10I RCCGGY 1 cut(s) 227
Cfr13I GGNCC 3 cut(s) 238, 418, 674
Csp6I GTAC 3 cut(s) 631, 875, 1267
CviAII CATG 8 cut(s) 191, 765, 868, 899, 965, 977, 1006, 1205
CviQI GTAC 3 cut(s) 631, 875, 1267
DdeI CTNAG 4 cut(s) 291, 938, 1011, 1116
DpnI GATC 5 cut(s) 273, 473, 596, 1023, 1200
DpnII GATC 5 cut(s) 271, 471, 594, 1021, 1198
EaeI YGGCCR 1 cut(s) 448
Eco105I TACGTA 1 cut(s) 1266
Eco130I CCWWGG 2 cut(s) 15, 196
Eco147I AGGCCT 1 cut(s) 943
Eco47I GGWCC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 408
Eco88I CYCGRG 1 cut(s) 411
Eco91I GGTNACC 1 cut(s) 832
EcoO109I RGGNCCY 2 cut(s) 238, 674
EcoO65I GGTNACC 1 cut(s) 832
EcoRII CCWGG 2 cut(s) 697, 775
EcoT14I CCWWGG 2 cut(s) 15, 196
EcoT22I ATGCAT 2 cut(s) 323, 766
ErhI CCWWGG 2 cut(s) 15, 196
FaeI CATG 8 cut(s) 194, 768, 871, 902, 968, 980, 1009, 1208
FaqI GGGAC 1 cut(s) 808
FatI CATG 8 cut(s) 190, 764, 867, 898, 964, 976, 1005, 1204
FauI CCCGC 1 cut(s) 707
FauNDI CATATG 2 cut(s) 7, 337
FblI GTMKAC 1 cut(s) 1259
Fnu4HI GCNGC 5 cut(s) 204, 288, 728, 855, 1217
FokI GGATG 2 cut(s) 353, 483
Fsp4HI GCNGC 5 cut(s) 204, 288, 728, 855, 1217
FspBI CTAG 1 cut(s) 851
GluI GCNGC 5 cut(s) 204, 288, 728, 855, 1217
GsuI CTGGAG 1 cut(s) 270
HaeIII GGCC 4 cut(s) 420, 450, 675, 943
HapII CCGG 2 cut(s) 228, 1127
Hin1II CATG 8 cut(s) 194, 768, 871, 902, 968, 980, 1009, 1208
HincII GTYRAC 1 cut(s) 1224
HindII GTYRAC 1 cut(s) 1224
HinfI GANTC 6 cut(s) 295, 329, 381, 772, 905, 1009
HpaII CCGG 2 cut(s) 228, 1127
HphI GGTGA 3 cut(s) 630, 826, 1292
Hpy166II GTNNAC 3 cut(s) 1154, 1224, 1260
Hpy188I TCNGA 4 cut(s) 751, 915, 1182, 1195
Hpy188III TCNNGA 7 cut(s) 411, 469, 500, 685, 977, 1006, 1019
Hpy8I GTNNAC 3 cut(s) 1154, 1224, 1260
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 3 cut(s) 628, 981, 1101
HpyCH4III ACNGT 2 cut(s) 148, 303
HpyCH4IV ACGT 1 cut(s) 1265
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpyF3I CTNAG 4 cut(s) 291, 938, 1011, 1116
HpySE526I ACGT 1 cut(s) 1265
Hsp92II CATG 8 cut(s) 194, 768, 871, 902, 968, 980, 1009, 1208
Kzo9I GATC 5 cut(s) 271, 471, 594, 1021, 1198
LmnI GCTCC 2 cut(s) 862, 934
Lsp1109I GCAGC 5 cut(s) 190, 274, 739, 841, 1228
MaeI CTAG 1 cut(s) 851
MaeII ACGT 1 cut(s) 1265
MaeIII GTNAC 2 cut(s) 832, 1234
MalI GATC 5 cut(s) 273, 473, 596, 1023, 1200
MbiI CCGCTC 1 cut(s) 862
MboI GATC 5 cut(s) 271, 471, 594, 1021, 1198
MboII GAAGA 8 cut(s) 86, 278, 281, 522, 698, 1132, 1193, 1208
MflI RGATCY 2 cut(s) 594, 1198
MhlI GDGCHC 2 cut(s) 457, 749
MluCI AATT 4 cut(s) 120, 429, 658, 1051
MlyI GAGTC 3 cut(s) 289, 899, 1003
MmeI TCCRAC 1 cut(s) 1114
Mph1103I ATGCAT 2 cut(s) 323, 766
MroXI GAANNNNTTC 2 cut(s) 380, 1200
MseI TTAA 3 cut(s) 102, 428, 627
MslI CAYNNNNRTG 2 cut(s) 282, 839
MspA1I CMGCKG 1 cut(s) 1219
MspI CCGG 2 cut(s) 228, 1127
MspR9I CCNGG 3 cut(s) 699, 777, 1127
Mva1269I GAATGC 1 cut(s) 162
MvaI CCWGG 2 cut(s) 699, 777
MvnI CGCG 1 cut(s) 264
MwoI GCNNNNNNNGC 1 cut(s) 17
NciI CCSGG 1 cut(s) 1127
NdeI CATATG 2 cut(s) 7, 337
NdeII GATC 5 cut(s) 271, 471, 594, 1021, 1198
NheI GCTAGC 1 cut(s) 850
NlaIII CATG 8 cut(s) 194, 768, 871, 902, 968, 980, 1009, 1208
NlaIV GGNNCC 4 cut(s) 239, 419, 596, 676
NmuCI GTSAC 2 cut(s) 832, 1234
NsiI ATGCAT 2 cut(s) 323, 766
NspI RCATGY 2 cut(s) 768, 871
OliI CACNNNNGTG 1 cut(s) 282
PaeR7I CTCGAG 1 cut(s) 411
PagI TCATGA 2 cut(s) 976, 1005
PceI AGGCCT 1 cut(s) 943
PciI ACATGT 1 cut(s) 867
PcsI WCGNNNNNNNCGW 3 cut(s) 280, 367, 418
PctI GAATGC 1 cut(s) 162
PdmI GAANNNNTTC 2 cut(s) 380, 1200
PfeI GAWTC 3 cut(s) 329, 381, 772
PkrI GCNGC 5 cut(s) 205, 289, 729, 856, 1218
PleI GAGTC 3 cut(s) 289, 899, 1003
PpsI GAGTC 3 cut(s) 289, 899, 1003
Ppu21I YACGTR 1 cut(s) 1266
PpuMI RGGWCCY 1 cut(s) 238
PscI ACATGT 1 cut(s) 867
PshBI ATTAAT 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 238
Psp6I CCWGG 2 cut(s) 697, 775
PspEI GGTNACC 1 cut(s) 832
PspGI CCWGG 2 cut(s) 697, 775
PspN4I GGNNCC 4 cut(s) 239, 419, 596, 676
PspPI GGNCC 3 cut(s) 238, 418, 674
PspPPI RGGWCCY 1 cut(s) 238
PstI CTGCAG 1 cut(s) 187
PstNI CAGNNNCTG 1 cut(s) 908
PsuI RGATCY 2 cut(s) 594, 1198
PvuII CAGCTG 1 cut(s) 1219
RsaI GTAC 3 cut(s) 632, 876, 1268
RsaNI GTAC 3 cut(s) 631, 875, 1267
RseI CAYNNNNRTG 2 cut(s) 282, 839
SaqAI TTAA 3 cut(s) 102, 428, 627
SatI GCNGC 5 cut(s) 204, 288, 728, 855, 1217
Sau3AI GATC 5 cut(s) 271, 471, 594, 1021, 1198
Sau96I GGNCC 3 cut(s) 238, 418, 674
SchI GAGTC 3 cut(s) 289, 899, 1003
ScrFI CCNGG 3 cut(s) 699, 777, 1127
SduI GDGCHC 2 cut(s) 457, 749
SfcI CTRYAG 1 cut(s) 183
Sfr274I CTCGAG 1 cut(s) 411
SinI GGWCC 1 cut(s) 238
SlaI CTCGAG 1 cut(s) 411
SmiMI CAYNNNNRTG 2 cut(s) 282, 839
SmlI CTYRAG 1 cut(s) 411
SmoI CTYRAG 1 cut(s) 411
SnaBI TACGTA 1 cut(s) 1266
Sse9I AATT 4 cut(s) 120, 429, 658, 1051
SseBI AGGCCT 1 cut(s) 943
SsiI CCGC 4 cut(s) 598, 714, 860, 1149
SspI AATATT 1 cut(s) 514
SspMI CTAG 1 cut(s) 851
StuI AGGCCT 1 cut(s) 943
StyD4I CCNGG 3 cut(s) 697, 775, 1125
StyI CCWWGG 2 cut(s) 15, 196
TaaI ACNGT 2 cut(s) 148, 303
TaiI ACGT 1 cut(s) 1268
TaqI TCGA 4 cut(s) 87, 274, 361, 412
TaqII GACCGA 1 cut(s) 670
TasI AATT 4 cut(s) 120, 429, 658, 1051
TatI WGTACW 1 cut(s) 630
TfiI GAWTC 3 cut(s) 329, 381, 772
Tru1I TTAA 3 cut(s) 102, 428, 627
Tru9I TTAA 3 cut(s) 102, 428, 627
TscAI CASTG 3 cut(s) 187, 391, 406
TseFI GTSAC 2 cut(s) 832, 1234
TseI GCWGC 5 cut(s) 203, 287, 727, 854, 1216
Tsp45I GTSAC 2 cut(s) 832, 1234
TspDTI ATGAA 6 cut(s) 599, 834, 857, 876, 953, 1193
TspGWI ACGGA 2 cut(s) 272, 359
TspRI CASTG 3 cut(s) 187, 391, 406
VpaK11BI GGWCC 1 cut(s) 238
VspI ATTAAT 1 cut(s) 102
XapI RAATTY 1 cut(s) 658
XceI RCATGY 2 cut(s) 768, 871
XhoI CTCGAG 1 cut(s) 411
XmiI GTMKAC 1 cut(s) 1259
XmnI GAANNNNTTC 2 cut(s) 380, 1200
XspI CTAG 1 cut(s) 851
Zsp2I ATGCAT 2 cut(s) 323, 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.