Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002312

Physical Location & Seq

Genomic Coordinates Forward (+)

30474151 .. 30478017

Gene Structure 3867 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1237400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2091 bp

ATGGCTTCAATGGGACAACCCCCGCCGCCGCTGCCTCTTAAGCGGCGGGAGGCTCCTGCAGTATCAAGAGAAGATGATAAAATGATCATTACTCCACTCGGTGCTGGCAATGAAGTCGGCCGCTCTTGCGTGCACATGACCTTCAAAGGGAAAACTGTCATGTTTGATTGTGGAATTCACCCTGCTTATTCGGGCATGGCTGCCTTGCCTTACTTTGATGAGATTGATCCTTCCACAATTGATGTCCTCCTCGTAACCCACTTTCACTTGGATCATGCTGCATCCCTACCTTACTTTCTTGAGAAGACCACATTCAAAGGCCGAGTTTTTATGACTTATGCTACAAAGGCTATCTACAAGTTGCTTTTGACGGATTATATAAAAGTGAGCAAAGTTTCAGTTGAAGATATGTTGTTTGATGAGCAAGACATAAATTCCTCGATGGATAAAATTGAGGTTATTGATTTCCATCAGACAGTAGAGATTGAGGGCATTCGATTTTGGTGCTACACTGCCGGTCATGTCCTTGGTGCTGCCATGTTTATGGTTGATATAGCTGGTGTTAGAGTTCTTTATACTGGAGATTATTCACGTGAAGAAGACCGGCATCTCCGTGCTGCTGAGACCCCAGAGTTCTCCCCTGATGTGTGCATTATTGAATCCACTTATGGTGTCCAGCACCATCAACCTAGGCACATCCGTGAGAAGCGGTTCACTGATGTTATCCACACAACCGTCGCTCAAGGGGGTCGTGTCTTGATTCCTGTATTTGCACTTGGTCGTGCCCAAGAACTTCTCCTGATCCTTGATGAGTATTGGTCAAACCATCCTGAGCTCCACAATATTCCTATTTATTATGCTTCCCCACTTGCTAAAAGATGTTTGTCTGTATACGAGACATACACCCTTTCTATGAATGATAGGATCCGCAATGCGAAGTCAAATCCTTTCATTTTCAAGCACATATCACCACTAAAGAGCATTGAGAATCTCAAAGATGTAGGTCCATCGGTGGTGATGGCAAGCCCTGGTGGTCTTCAGAGTGGACTGTCAAGGCAGCTCTTTGATAAATGGTGCACTGATAAGAAAAATTCATGCGTTATTCCAGGTTATGTGGTAGAAGGGACTCTAGCCAAAACCATCATCAATGAACCAAAAGAGGTAACTAGTATGACTGGACTCTCGGTACCTCTCAACATGCAGGTGCATTACATTTCATTCTCTGCCCACGCAGACTCTGTTCAGACTAGTGCATTTTTGGAGGAGCTGAGGCCTCCTAACATAATCCTAGTTCATGGAGAAGCTAATGAGATGGGAAGGCTAAAACAGAAGCTTGTGACCCAGTTTGCTGATCGGAACACCAAAATCCTGACCCCAAAGAATTGTCAGTCTGTTGAAATGTATTTTAACTCCCAGAAAATGGCAAAAGCTATTGGAAGGCTTGCTGAGAAGACCCCTGAAGTTGGTGAAAGTGTCAGCGGTTTACTGGTAAAAAAAGGCTTCAGTTATCAAATAATGGCTTCCGATGATCTTCATGTCTTCTCACAGCTGTCCACAGCAAATGTCACTCAACGGATTACTATTCCATATGCTAGTGGCTTCACTGTGATAAAGCATCGGCTTAAGCAAATATACGAGAGTGTAGAGTCTTCAGTGGATGAGGAATCTGGAGTGCCAACACTGCGAGTGCATGATCGGGTGACTTTGAAGCAGGATACAGATAAACACATATCACTGCATTGGGCGTCAGATCCTATAAGTGACATGGTGTCAGACTCCGTTGTTGCTCTTATTCTGAATATCAATCGAGAAGTCCCCAAGGTAGTTGTTGAATCAGAGGATATAAAAACTGAGGAAGAGAATGTGAAGAAGGCGGAAAAGGTTATCCATGCACTTCTTGTTTCACTCTTTGGCGACGTGAAGCCTGGAGAAAACGGGAAACTGGTGATTAGTGTTGATGGGAATGTGGCGCAGCTTGATAAACAGAGCGGGGATGTAGAGAGTGAAAACGAAGGTCTCAAGGAAAGAGTAAAAACAGCATTTCGTCGAATCCAAAGTGCGGTGAAGCCAATCCCTCTCCGTGCAACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016073 GO:0016180 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

Beta-Casp CPSF73-100_C Lactamase_B Lactamase_B_6 RMMBL

Protein Analysis

697

Amino Acids

77.76

Weight (kDa)

6.26

Isoelectric Point (pI)

42.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lactamase_B	PF00753	43 - 202	3.2e-18	Metallo-beta-lactamase superfamily
Anti-Pycsar_Apyc1	PF23023	45 - 202	7.1e-09	Anti-Pycsar protein Apyc1
Lactamase_B_6	PF16661	46 - 209	3.1e-17	Metallo-beta-lactamase superfamily domain
Lactamase_B_2	PF12706	53 - 204	3.7e-06	Beta-lactamase superfamily domain
Beta-Casp	PF10996	262 - 381	8.9e-30	Beta-Casp domain
RMMBL	PF07521	396 - 448	1.1e-13	Zn-dependent metallo-hydrolase RNA specificity domain
CPSF73-100_C	PF11718	491 - 690	1e-51	Pre-mRNA 3'-end-processing endonuclease polyadenylation factor C-term

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002312)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61010 AT1G61010 AT1G61010 AT1G61010 AT1G61010
fragaria_vesca	FvH4_4g07000 FvH4_4g07000 FvH4_5g28761 FvH4_5g28770 FvH4_7g26770
malus_domestica	MD08G1237400.v1.1 MD15G1429300.v1.1
prunus_persica	Prupe.1G574800_v2.0.a1 Prupe.1G574800_v2.0.a1 Prupe.1G574900_v2.0.a1 Prupe.I006100_v2.0.a1 Prupe.I006100_v2.0.a1 Prupe.I006200_v2.0.a1
pyrus_communis	pycom08g20590 pycom08g20600 pycom15g37980 pycom15g37990
rosa_chinensis	RchiOBHm_Chr1g0363271 RchiOBHm_Chr4g0399131 RchiOBHm_Chr7g0220981
rosa_laevigata	RLG00000002186 RLG00000009298
rosa_multiflora	Rmu_sc0002002.1_g000015 Rmu_sc0002105.1_g000022
rosa_roxburghii	Rroxscaffold_3G00238830 Rroxscaffold_5G00344090
rosa_rugosa	Rorug04G0014100 Rorug07G0199100
rosa_samantha	Rh1DG309200 Rh4BG089900 Rh4DG084500 Rh7AG339000 Rh7BG329800 Rh7CG357100 Rh7DG337800
rosa_wichuraiana	Rw4G007590 Rw7G028840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1192
Acc36I	ACCTGC	1 cut(s)	1192
Acc65I	GGTACC	1 cut(s)	1186
AccB1I	GGYRCC	1 cut(s)	1186
AccB7I	CCANNNNNTGG	1 cut(s)	1420
AccBSI	CCGCTC	2 cut(s)	123, 1989
AccI	GTMKAC	1 cut(s)	891
AclWI	GGATC	6 cut(s)	221, 279, 796, 919, 932, 1745
AcoI	YGGCCR	1 cut(s)	118
AcsI	RAATTY	3 cut(s)	174, 433, 1090
AcuI	CTGAAG	4 cut(s)	1022, 1479, 1486, 1635
AcvI	CACGTG	1 cut(s)	593
AcyI	GRCGYC	1 cut(s)	1745
AdeI	CACNNNGTG	1 cut(s)	101
AfaI	GTAC	1 cut(s)	1188
AfiI	CCNNNNNNNGG	4 cut(s)	147, 1420, 1463, 2059
AflII	CTTAAG	2 cut(s)	38, 1622
AgsI	TTSAA	9 cut(s)	9, 145, 316, 404, 659, 958, 1397, 1708, 1832
AhdI	GACNNNNNGTC	1 cut(s)	1768
AhlI	ACTAGT	2 cut(s)	1166, 1247
AjiI	CACGTC	1 cut(s)	1918
AjnI	CCWGG	3 cut(s)	1027, 1105, 1924
AleI	CACNNNNGTG	1 cut(s)	699
AluBI	AGCT	9 cut(s)	557, 835, 1060, 1267, 1304, 1333, 1430, 1549, 1975
AluI	AGCT	9 cut(s)	557, 835, 1060, 1267, 1304, 1333, 1430, 1549, 1975
Alw21I	GWGCWC	3 cut(s)	135, 837, 1079
Alw26I	GTCTC	3 cut(s)	617, 890, 2021
Alw44I	GTGCAC	2 cut(s)	131, 1075
AlwI	GGATC	6 cut(s)	221, 279, 796, 919, 932, 1745
AlwNI	CAGNNNCTG	1 cut(s)	1238
AoxI	GGCC	3 cut(s)	118, 319, 1271
ApaLI	GTGCAC	2 cut(s)	131, 1075
ApeKI	GCWGC	7 cut(s)	31, 200, 278, 533, 617, 1057, 1972
ApoI	RAATTY	3 cut(s)	174, 433, 1090
Asp700I	GAANNNNTTC	1 cut(s)	710
Asp718I	GGTACC	1 cut(s)	1186
AspA2I	CCTAGG	1 cut(s)	689
AspLEI	GCGC	1 cut(s)	1972
AspS9I	GGNCC	1 cut(s)	1004
AsuHPI	GGTGA	7 cut(s)	170, 960, 1027, 1478, 1711, 1957, 2074
AvaII	GGWCC	1 cut(s)	1004
AvrII	CCTAGG	1 cut(s)	689
BaeGI	GKGCMC	3 cut(s)	135, 787, 1079
BamHI	GGATCC	1 cut(s)	924
BanI	GGYRCC	1 cut(s)	1186
BanII	GRGCYC	1 cut(s)	837
BbrPI	CACGTG	1 cut(s)	593
BbsI	GAAGAC	6 cut(s)	311, 606, 1028, 1457, 1531, 1641
Bbv12I	GWGCWC	3 cut(s)	135, 837, 1079
BbvCI	CCTCAGC	1 cut(s)	1268
BbvI	GCAGC	7 cut(s)	18, 187, 265, 520, 604, 1069, 1984
BccI	CCATC	9 cut(s)	436, 477, 690, 834, 1012, 1015, 1148, 1306, 1952
BcgI	CGANNNNNNTGC	4 cut(s)	97, 131, 486, 520
BciT130I	CCWGG	3 cut(s)	1029, 1107, 1926
BciVI	GTATCC	1 cut(s)	1708
BclI	TGATCA	1 cut(s)	84
BcoDI	GTCTC	3 cut(s)	617, 890, 2021
BcuI	ACTAGT	2 cut(s)	1166, 1247
BfaI	CTAG	6 cut(s)	690, 1130, 1167, 1248, 1289, 1593
BfmI	CTRYAG	1 cut(s)	57
BfrI	CTTAAG	2 cut(s)	38, 1622
BfuAI	ACCTGC	1 cut(s)	1192
BfuI	GTATCC	1 cut(s)	1708
BlnI	CCTAGG	1 cut(s)	689
Bme1390I	CCNGG	3 cut(s)	1029, 1107, 1926
Bme18I	GGWCC	1 cut(s)	1004
BmeRI	GACNNNNNGTC	1 cut(s)	1768
BmgBI	CACGTC	1 cut(s)	1918
BmgT120I	GGNCC	1 cut(s)	1004
BmiI	GGNNCC	3 cut(s)	54, 926, 1188
BmrFI	CCNGG	3 cut(s)	1029, 1107, 1926
BmrI	ACTGGG	1 cut(s)	1336
BmsI	GCATC	3 cut(s)	290, 616, 1624
BmuI	ACTGGG	1 cut(s)	1336
BpiI	GAAGAC	6 cut(s)	311, 606, 1028, 1457, 1531, 1641
BpmI	CTGGAG	3 cut(s)	600, 1689, 1947
Bpu10I	CCTNAGC	2 cut(s)	831, 1268
BpuEI	CTTGAG	3 cut(s)	320, 726, 2003
BsaAI	YACGTR	1 cut(s)	593
BsaBI	GATNNNNATC	1 cut(s)	468
BsaHI	GRCGYC	1 cut(s)	1745
BsaI	GGTCTC	2 cut(s)	617, 2021
BsaJI	CCNNGG	4 cut(s)	526, 689, 1027, 1818
Bsc4I	CCNNNNNNNGG	4 cut(s)	147, 1420, 1463, 2059
Bse118I	RCCGGY	2 cut(s)	515, 603
Bse1I	ACTGG	5 cut(s)	583, 1180, 1342, 1491, 1947
Bse3DI	GCAATG	2 cut(s)	115, 937
Bse8I	GATNNNNATC	1 cut(s)	468
BseBI	CCWGG	3 cut(s)	1029, 1107, 1926
BseDI	CCNNGG	4 cut(s)	526, 689, 1027, 1818
BseGI	GGATG	5 cut(s)	281, 696, 826, 1663, 1999
BseJI	GATNNNNATC	1 cut(s)	468
BseLI	CCNNNNNNNGG	4 cut(s)	147, 1420, 1463, 2059
BseMI	GCAATG	2 cut(s)	115, 937
BseMII	CTCAG	5 cut(s)	612, 822, 1259, 1437, 1842
BseNI	ACTGG	5 cut(s)	583, 1180, 1342, 1491, 1947
BseRI	GAGGAG	2 cut(s)	239, 1277
BseSI	GKGCMC	3 cut(s)	135, 787, 1079
BseX3I	CGGCCG	1 cut(s)	118
BseXI	GCAGC	7 cut(s)	18, 187, 265, 520, 604, 1069, 1984
Bsh1285I	CGRYCG	1 cut(s)	121
BshFI	GGCC	3 cut(s)	120, 321, 1273
BshNI	GGYRCC	1 cut(s)	1186
BsiEI	CGRYCG	1 cut(s)	121
BsiHKAI	GWGCWC	3 cut(s)	135, 837, 1079
BsiSI	CCGG	2 cut(s)	516, 604
BslFI	GGGAC	3 cut(s)	27, 1138, 1799
BslI	CCNNNNNNNGG	4 cut(s)	147, 1420, 1463, 2059
BsmAI	GTCTC	3 cut(s)	617, 890, 2021
BsmFI	GGGAC	3 cut(s)	27, 1138, 1799
BsmI	GAATGC	1 cut(s)	492
BsnI	GGCC	3 cut(s)	120, 321, 1273
Bso31I	GGTCTC	2 cut(s)	617, 2021
Bsp1286I	GDGCHC	4 cut(s)	135, 787, 837, 1079
Bsp143I	GATC	9 cut(s)	84, 226, 271, 801, 924, 1351, 1528, 1693, 1750
BspANI	GGCC	3 cut(s)	120, 321, 1273
BspCNI	CTCAG	5 cut(s)	613, 823, 1260, 1438, 1843
BspLI	GGNNCC	3 cut(s)	54, 926, 1188
BspMAI	CTGCAG	1 cut(s)	61
BspMI	ACCTGC	1 cut(s)	1192
BspPI	GGATC	6 cut(s)	221, 279, 796, 919, 932, 1745
BspT107I	GGYRCC	1 cut(s)	1186
BspTI	CTTAAG	2 cut(s)	38, 1622
BspTNI	GGTCTC	2 cut(s)	617, 2021
BsrBI	CCGCTC	2 cut(s)	123, 1989
BsrDI	GCAATG	2 cut(s)	115, 937
BsrFI	RCCGGY	2 cut(s)	515, 603
BsrI	ACTGG	5 cut(s)	583, 1180, 1342, 1491, 1947
BssAI	RCCGGY	2 cut(s)	515, 603
BssECI	CCNNGG	4 cut(s)	526, 689, 1027, 1818
BssMI	GATC	9 cut(s)	84, 226, 271, 801, 924, 1351, 1528, 1693, 1750
BssNAI	GTATAC	1 cut(s)	892
BssNI	GRCGYC	1 cut(s)	1745
BssT1I	CCWWGG	3 cut(s)	526, 689, 1818
Bst1107I	GTATAC	1 cut(s)	892
Bst2UI	CCWGG	3 cut(s)	1029, 1107, 1926
Bst4CI	ACNGT	5 cut(s)	157, 478, 736, 1050, 1606
Bst6I	CTCTTC	1 cut(s)	1851
BstACI	GRCGYC	1 cut(s)	1745
BstAFI	CTTAAG	2 cut(s)	38, 1622
BstBAI	YACGTR	1 cut(s)	593
BstC8I	GCNNGC	4 cut(s)	106, 131, 1024, 1443
BstDEI	CTNAG	6 cut(s)	621, 831, 1268, 1446, 1851, 2088
BstF5I	GGATG	5 cut(s)	281, 696, 826, 1663, 1999
BstHHI	GCGC	1 cut(s)	1972
BstKTI	GATC	9 cut(s)	87, 229, 274, 804, 927, 1354, 1531, 1696, 1753
BstMAI	GTCTC	3 cut(s)	617, 890, 2021
BstMBI	GATC	9 cut(s)	84, 226, 271, 801, 924, 1351, 1528, 1693, 1750
BstMCI	CGRYCG	1 cut(s)	121
BstMWI	GCNNNNNNNGC	5 cut(s)	31, 40, 126, 347, 1681
BstNI	CCWGG	3 cut(s)	1029, 1107, 1926
BstNSI	RCATGY	1 cut(s)	1201
BstSCI	CCNGG	3 cut(s)	1027, 1105, 1924
BstSFI	CTRYAG	1 cut(s)	57
BstSLI	GKGCMC	3 cut(s)	135, 787, 1079
BstV1I	GCAGC	7 cut(s)	18, 187, 265, 520, 604, 1069, 1984
BstV2I	GAAGAC	6 cut(s)	311, 606, 1028, 1457, 1531, 1641
BstX2I	RGATCY	2 cut(s)	924, 1750
BstXI	CCANNNNNNTGG	1 cut(s)	544
BstYI	RGATCY	2 cut(s)	924, 1750
BstZ17I	GTATAC	1 cut(s)	892
BstZI	CGGCCG	1 cut(s)	118
BsuI	GTATCC	1 cut(s)	1708
BsuRI	GGCC	3 cut(s)	120, 321, 1273
BtrI	CACGTC	1 cut(s)	1918
BtsCI	GGATG	5 cut(s)	281, 696, 826, 1663, 1999
BtsI	GCAGTG	3 cut(s)	510, 1679, 1733
BtsIMutI	CAGTG	7 cut(s)	510, 714, 1077, 1602, 1659, 1679, 1733
BveI	ACCTGC	1 cut(s)	1192
Cac8I	GCNNGC	4 cut(s)	106, 131, 1024, 1443
CaiI	CAGNNNCTG	1 cut(s)	1238
CfoI	GCGC	1 cut(s)	1972
Cfr10I	RCCGGY	2 cut(s)	515, 603
Cfr13I	GGNCC	1 cut(s)	1004
CseI	GACGC	1 cut(s)	1734
Csp6I	GTAC	1 cut(s)	1187
CviQI	GTAC	1 cut(s)	1187
DdeI	CTNAG	6 cut(s)	621, 831, 1268, 1446, 1851, 2088
DpnI	GATC	9 cut(s)	86, 228, 273, 803, 926, 1353, 1530, 1695, 1752
DpnII	GATC	9 cut(s)	84, 226, 271, 801, 924, 1351, 1528, 1693, 1750
DraIII	CACNNNGTG	1 cut(s)	101
DriI	GACNNNNNGTC	1 cut(s)	1768
EaeI	YGGCCR	1 cut(s)	118
EagI	CGGCCG	1 cut(s)	118
Eam1104I	CTCTTC	1 cut(s)	1851
Eam1105I	GACNNNNNGTC	1 cut(s)	1768
EarI	CTCTTC	1 cut(s)	1851
EciI	GGCGGA	1 cut(s)	1889
Ecl136II	GAGCTC	1 cut(s)	835
EclXI	CGGCCG	1 cut(s)	118
Eco130I	CCWWGG	3 cut(s)	526, 689, 1818
Eco147I	AGGCCT	1 cut(s)	1273
Eco24I	GRGCYC	1 cut(s)	837
Eco31I	GGTCTC	2 cut(s)	617, 2021
Eco47I	GGWCC	1 cut(s)	1004
Eco52I	CGGCCG	1 cut(s)	118
Eco53kI	GAGCTC	1 cut(s)	835
Eco57I	CTGAAG	4 cut(s)	1022, 1479, 1486, 1635
Eco72I	CACGTG	1 cut(s)	593
EcoICRI	GAGCTC	1 cut(s)	835
EcoRI	GAATTC	1 cut(s)	174
EcoRII	CCWGG	3 cut(s)	1027, 1105, 1924
EcoT14I	CCWWGG	3 cut(s)	526, 689, 1818
EcoT38I	GRGCYC	1 cut(s)	837
ErhI	CCWWGG	3 cut(s)	526, 689, 1818
FaqI	GGGAC	3 cut(s)	27, 1138, 1799
FauI	CCCGC	3 cut(s)	30, 39, 1982
FauNDI	CATATG	1 cut(s)	1588
FbaI	TGATCA	1 cut(s)	84
FblI	GTMKAC	1 cut(s)	891
FokI	GGATG	5 cut(s)	268, 683, 813, 1670, 2006
FriOI	GRGCYC	1 cut(s)	837
FspBI	CTAG	6 cut(s)	690, 1130, 1167, 1248, 1289, 1593
GlaI	GCGC	1 cut(s)	1971
GsuI	CTGGAG	3 cut(s)	600, 1689, 1947
HaeIII	GGCC	3 cut(s)	120, 321, 1273
HapII	CCGG	2 cut(s)	516, 604
HgaI	GACGC	1 cut(s)	1734
HhaI	GCGC	1 cut(s)	1972
Hin1I	GRCGYC	1 cut(s)	1745
Hin6I	GCGC	1 cut(s)	1970
HinP1I	GCGC	1 cut(s)	1970
HindIII	AAGCTT	1 cut(s)	1331
HpaII	CCGG	2 cut(s)	516, 604
HphI	GGTGA	7 cut(s)	170, 960, 1027, 1478, 1711, 1957, 2074
Hpy166II	GTNNAC	7 cut(s)	133, 714, 892, 1046, 1077, 1484, 1554
Hpy188I	TCNGA	9 cut(s)	474, 1041, 1245, 1356, 1525, 1750, 1774, 1797, 1837
Hpy188III	TCNNGA	8 cut(s)	66, 299, 757, 799, 830, 1369, 1668, 1808
Hpy8I	GTNNAC	7 cut(s)	133, 714, 892, 1046, 1077, 1484, 1554
Hpy99I	CGWCG	3 cut(s)	740, 1919, 2049
HpyAV	CCTTC	7 cut(s)	151, 240, 1115, 1311, 1431, 1864, 2006
HpyCH4III	ACNGT	5 cut(s)	157, 478, 736, 1050, 1606
HpyCH4IV	ACGT	2 cut(s)	592, 1917
HpyF10VI	GCNNNNNNNGC	5 cut(s)	31, 40, 126, 347, 1681
HpyF3I	CTNAG	6 cut(s)	621, 831, 1268, 1446, 1851, 2088
HpySE526I	ACGT	2 cut(s)	592, 1917
Hsp92I	GRCGYC	1 cut(s)	1745
HspAI	GCGC	1 cut(s)	1970
KpnI	GGTACC	1 cut(s)	1190
Ksp22I	TGATCA	1 cut(s)	84
Kzo9I	GATC	9 cut(s)	84, 226, 271, 801, 924, 1351, 1528, 1693, 1750
LmnI	GCTCC	3 cut(s)	58, 840, 1264
Lsp1109I	GCAGC	7 cut(s)	18, 187, 265, 520, 604, 1069, 1984
LweI	GCATC	3 cut(s)	290, 616, 1624
MaeI	CTAG	6 cut(s)	690, 1130, 1167, 1248, 1289, 1593
MaeII	ACGT	2 cut(s)	592, 1917
MaeIII	GTNAC	6 cut(s)	253, 1162, 1336, 1564, 1699, 1760
MalI	GATC	9 cut(s)	86, 228, 273, 803, 926, 1353, 1530, 1695, 1752
MbiI	CCGCTC	2 cut(s)	123, 1989
MboI	GATC	9 cut(s)	84, 226, 271, 801, 924, 1351, 1528, 1693, 1750
MfeI	CAATTG	1 cut(s)	237
MflI	RGATCY	2 cut(s)	924, 1750
MhlI	GDGCHC	4 cut(s)	135, 787, 837, 1079
MluCI	AATT	6 cut(s)	174, 237, 433, 450, 1090, 1381
MlyI	GAGTC	5 cut(s)	1120, 1173, 1229, 1655, 1769
MroXI	GAANNNNTTC	1 cut(s)	710
MseI	TTAA	3 cut(s)	39, 1407, 1623
MslI	CAYNNNNRTG	4 cut(s)	542, 612, 699, 1202
MspA1I	CMGCKG	3 cut(s)	31, 1479, 1549
MspCI	CTTAAG	2 cut(s)	38, 1622
MspI	CCGG	2 cut(s)	516, 604
MspR9I	CCNGG	3 cut(s)	1029, 1107, 1926
MunI	CAATTG	1 cut(s)	237
Mva1269I	GAATGC	1 cut(s)	492
MvaI	CCWGG	3 cut(s)	1029, 1107, 1926
MwoI	GCNNNNNNNGC	5 cut(s)	31, 40, 126, 347, 1681
NdeI	CATATG	1 cut(s)	1588
NdeII	GATC	9 cut(s)	84, 226, 271, 801, 924, 1351, 1528, 1693, 1750
NlaIV	GGNNCC	3 cut(s)	54, 926, 1188
NmeAIII	GCCGAG	1 cut(s)	347
NmuCI	GTSAC	4 cut(s)	1336, 1564, 1699, 1760
NspI	RCATGY	1 cut(s)	1201
OliI	CACNNNNGTG	1 cut(s)	699
PaqCI	CACCTGC	1 cut(s)	1192
PceI	AGGCCT	1 cut(s)	1273
PctI	GAATGC	1 cut(s)	492
PdmI	GAANNNNTTC	1 cut(s)	710
PfeI	GAWTC	6 cut(s)	659, 760, 988, 1664, 1832, 2049
PflMI	CCANNNNNTGG	1 cut(s)	1420
PleI	GAGTC	5 cut(s)	1120, 1173, 1229, 1654, 1769
PmaCI	CACGTG	1 cut(s)	593
PmlI	CACGTG	1 cut(s)	593
PpsI	GAGTC	5 cut(s)	1120, 1173, 1229, 1654, 1769
Ppu21I	YACGTR	1 cut(s)	593
Psp124BI	GAGCTC	1 cut(s)	837
Psp6I	CCWGG	3 cut(s)	1027, 1105, 1924
PspCI	CACGTG	1 cut(s)	593
PspGI	CCWGG	3 cut(s)	1027, 1105, 1924
PspN4I	GGNNCC	3 cut(s)	54, 926, 1188
PspPI	GGNCC	1 cut(s)	1004
PstI	CTGCAG	1 cut(s)	61
PstNI	CAGNNNCTG	1 cut(s)	1238
PsuI	RGATCY	2 cut(s)	924, 1750
PvuII	CAGCTG	1 cut(s)	1549
RsaI	GTAC	1 cut(s)	1188
RsaNI	GTAC	1 cut(s)	1187
RseI	CAYNNNNRTG	4 cut(s)	542, 612, 699, 1202
SacI	GAGCTC	1 cut(s)	837
SaqAI	TTAA	3 cut(s)	39, 1407, 1623
Sau3AI	GATC	9 cut(s)	84, 226, 271, 801, 924, 1351, 1528, 1693, 1750
Sau96I	GGNCC	1 cut(s)	1004
SchI	GAGTC	5 cut(s)	1120, 1173, 1229, 1655, 1769
ScrFI	CCNGG	3 cut(s)	1029, 1107, 1926
SduI	GDGCHC	4 cut(s)	135, 787, 837, 1079
SfaNI	GCATC	3 cut(s)	290, 616, 1624
SfcI	CTRYAG	1 cut(s)	57
SinI	GGWCC	1 cut(s)	1004
SmiMI	CAYNNNNRTG	4 cut(s)	542, 612, 699, 1202
SmlI	CTYRAG	5 cut(s)	38, 299, 741, 1622, 2018
SmoI	CTYRAG	5 cut(s)	38, 299, 741, 1622, 2018
SpeI	ACTAGT	2 cut(s)	1166, 1247
Sse9I	AATT	6 cut(s)	174, 237, 433, 450, 1090, 1381
SseBI	AGGCCT	1 cut(s)	1273
SspI	AATATT	1 cut(s)	844
SspMI	CTAG	6 cut(s)	690, 1130, 1167, 1248, 1289, 1593
SstI	GAGCTC	1 cut(s)	837
StuI	AGGCCT	1 cut(s)	1273
StyD4I	CCNGG	3 cut(s)	1027, 1105, 1924
StyI	CCWWGG	3 cut(s)	526, 689, 1818
TaaI	ACNGT	5 cut(s)	157, 478, 736, 1050, 1606
TaiI	ACGT	2 cut(s)	595, 1920
TaqI	TCGA	4 cut(s)	440, 496, 1807, 2047
TasI	AATT	6 cut(s)	174, 237, 433, 450, 1090, 1381
TauI	GCSGC	4 cut(s)	28, 31, 46, 123
TfiI	GAWTC	6 cut(s)	659, 760, 988, 1664, 1832, 2049
Tru1I	TTAA	3 cut(s)	39, 1407, 1623
Tru9I	TTAA	3 cut(s)	39, 1407, 1623
TscAI	CASTG	7 cut(s)	517, 721, 1084, 1609, 1659, 1686, 1740
TseFI	GTSAC	4 cut(s)	1336, 1564, 1699, 1760
TseI	GCWGC	7 cut(s)	31, 200, 278, 533, 617, 1057, 1972
Tsp45I	GTSAC	4 cut(s)	1336, 1564, 1699, 1760
TspDTI	ATGAA	8 cut(s)	126, 929, 940, 1083, 1164, 1206, 1283, 1523
TspGWI	ACGGA	6 cut(s)	386, 602, 689, 1588, 1768, 2069
TspRI	CASTG	7 cut(s)	517, 721, 1084, 1609, 1659, 1686, 1740
Van91I	CCANNNNNTGG	1 cut(s)	1420
Vha464I	CTTAAG	2 cut(s)	38, 1622
VneI	GTGCAC	2 cut(s)	131, 1075
VpaK11BI	GGWCC	1 cut(s)	1004
XapI	RAATTY	3 cut(s)	174, 433, 1090
XceI	RCATGY	1 cut(s)	1201
XmaJI	CCTAGG	1 cut(s)	689
XmiI	GTMKAC	1 cut(s)	891
XmnI	GAANNNNTTC	1 cut(s)	710
XspI	CTAG	6 cut(s)	690, 1130, 1167, 1248, 1289, 1593

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.