Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002312

Physical Location & Seq

Genomic Coordinates Forward (+)

52983302 .. 52987228

Gene Structure 3927 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1429300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2094 bp

ATGGCTTCAGTGGGACAACCACCGCCGGCGCCGGCGCCTCTTAAGCGGCGGGAGGCTCCTGCAGCATCAAGAGAAGATGATAAGATGATCATCACTCCGCTAGGTGCCGGCAATGAAGTCGGCCGCTCTTGCGTGCACATGACCTTCAAAGGGAAAACTGTCTTGTTTGATTGTGGAATTCACCCCGCTTATTCGGGCATGGCTGCTTTGCCCTACTTTGATGAGATTGATCCGTCCACAATTGATGTCCTCCTCGTAACGCACTTTCACTTGGATCATGCTGCATCCCTACCTTACTTTCTTGAGAAGACCACATTCAAAGGCCGAGTTTTTATGACTTATGCTACAAAGGTTATCTACAAGTTGCTTTTGACGGATTATATAAAAGTGAGCAAAGTTTCAGTTGAAGATATGTTGTTTGACGAGCAAGACATAAATTCCTCAATGGATAGAATTGAGGTTATTGATTTCCATCAGACAGTAGAGGTTGAGGGCATTCGATTTTGGTGCTACGCTGCCGGTCATGTCCTTGGTGCTGCCATGTTTATGGTTGATATAGCCGGTGTTAGAGTCCTTTATACTGGAGATTATTCACGTGAAGAAGACCGGCATCTCCGTGCTGCTGAGACCCCACTGTTCTCCCCTGATGTATGCATTATTGAATCGACTTACGGTGTCCAGCACCATCAACCTCGGCACATCCGTGAGAAGCGGTTCACTGATGTTATCCACACAACCATCTCTCAGGGGGGTCGTGTCTTGATTCCTGCATATGCACTTGGCCGTGCCCAAGAACTTCTCCTCATCCTTGATGAGTATTGGTCAAACCATCCTGAGCTCCATAATATTCCCATTTATTATGCTTCTCCACTTGCCAAAAGATGTTTGTCTGTATACGAGACATACACCCTTTCTATGAACGATAGGATCCGCAATGCGAAGTCAAATCCTTTCATTTTTAAGTACATATCACCACTAAAGAGCATTGAGAATTTCAAAGATGTAGGCCCATCGGTGGTGATGGCAAGCCCTGGTGGTCTTCAGAGTGGACTGTCAAGGCAGCTATTTGATAAGTGGTGCACTGATAAGAAAAATTCCTGCGTTATTCCAGGTTATATGGTTGAAGGGACTCTAGCCAAAACCATCATCAATGAACCAAAAGAGGTAACTAGTATGAGTGGACTCTCGGTACCTCTCAACATGCAGGTCCATCACATTTCATTCTCCGCCCACGCGGACTCTGTTCAGACTACTGCATTTTTGGACGAGCTGAGGCCTCCTAACATAATCCTAGTTCATGGAGGAGCTAATGAGATGGGAAGGCTAAAACAGAAGCTCGTGACCCAGTTTGCTGATTGGAACACCAAAATCCTGACCCCAAAGAATTGTCAGTCTGTTGAATTGTATTTTAACTCCCAGAAAATGGCAAAAGCTATTGGAAGGCTTGCTGAGGAGACCCCTGAAGTTCGTGAAACTGTCAGCGGTTTGCTGGTAAAAAAGGGCTTCAGTTATCAAATAATGGCTTCCGATGATCTCCATGTCTTCTCACAGCTGTGCACAGCAAATGTCACTCAACGGATTACTATTCCATATGCTAGTGGCTTCACTGTGATAAAACATCGGCTTGAGCAAATATACGGGAGTGTAGAGTCTTCAGTGGATGAGGAATCTGGAGTGGCAACATTGCGAGTGCATGATCGGGTGACTTTGAAGCAGGATACAGATAAACACATATCACTGCATTGGCCTTCGGATCCTATAAGTGACATGGTGTCAGACTCCATTGTTGCTCTTATTTTGAATATCAATCGAGAAGTCCCCAAGGTAGTTGTTGAATCAGAGGATATAAAAACTGAGGAAGAAAATGGGAAGAAGGTGGAAAAGGTCATTCGTGCATTTCTTGTTTCACTCTTTGGCGACGTGAAGCCTGGAGAAAATGGGAAACTAGTGATTAGTGTTGATGGGAATGTGGCGCAGCTTGATAAACAGAGCGGGGATGTAGAGAGTGAAAACGAAGGTCTCAAGGAAAGAGTAAAAACAGCATTTCGTCGAATCCAAAGTGCGGTGAAGCCAATCCCTCTCCATGCAACGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016073 GO:0016180 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

Beta-Casp CPSF73-100_C Lactamase_B Lactamase_B_6 RMMBL

Protein Analysis

698

Amino Acids

77.81

Weight (kDa)

6.2

Isoelectric Point (pI)

42.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lactamase_B	PF00753	44 - 204	8e-18	Metallo-beta-lactamase superfamily
Anti-Pycsar_Apyc1	PF23023	46 - 203	2.3e-08	Anti-Pycsar protein Apyc1
Lactamase_B_6	PF16661	47 - 209	4.7e-17	Metallo-beta-lactamase superfamily domain
Lactamase_B_2	PF12706	53 - 223	8.1e-07	Beta-lactamase superfamily domain
Beta-Casp	PF10996	263 - 382	1.4e-29	Beta-Casp domain
RMMBL	PF07521	397 - 457	1.6e-12	Zn-dependent metallo-hydrolase RNA specificity domain
CPSF73-100_C	PF11718	492 - 691	5.9e-51	Pre-mRNA 3'-end-processing endonuclease polyadenylation factor C-term

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002312)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61010 AT1G61010 AT1G61010 AT1G61010 AT1G61010
fragaria_vesca	FvH4_4g07000 FvH4_4g07000 FvH4_5g28761 FvH4_5g28770 FvH4_7g26770
malus_domestica	MD08G1237400.v1.1 MD15G1429300.v1.1
prunus_persica	Prupe.1G574800_v2.0.a1 Prupe.1G574800_v2.0.a1 Prupe.1G574900_v2.0.a1 Prupe.I006100_v2.0.a1 Prupe.I006100_v2.0.a1 Prupe.I006200_v2.0.a1
pyrus_communis	pycom08g20590 pycom08g20600 pycom15g37980 pycom15g37990
rosa_chinensis	RchiOBHm_Chr1g0363271 RchiOBHm_Chr4g0399131 RchiOBHm_Chr7g0220981
rosa_laevigata	RLG00000002186 RLG00000009298
rosa_multiflora	Rmu_sc0002002.1_g000015 Rmu_sc0002105.1_g000022
rosa_roxburghii	Rroxscaffold_3G00238830 Rroxscaffold_5G00344090
rosa_rugosa	Rorug04G0014100 Rorug07G0199100
rosa_samantha	Rh1DG309200 Rh4BG089900 Rh4DG084500 Rh7AG339000 Rh7BG329800 Rh7CG357100 Rh7DG337800
rosa_wichuraiana	Rw4G007590 Rw7G028840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1195
Acc65I	GGTACC	1 cut(s)	1189
AccB1I	GGYRCC	4 cut(s)	28, 34, 104, 1189
AccB7I	CCANNNNNTGG	1 cut(s)	1423
AccBSI	CCGCTC	2 cut(s)	126, 1992
AccI	GTMKAC	1 cut(s)	894
AccII	CGCG	1 cut(s)	1235
AclWI	GGATC	6 cut(s)	224, 282, 922, 935, 1748, 1761
AcoI	YGGCCR	2 cut(s)	121, 781
AcsI	RAATTY	4 cut(s)	177, 436, 991, 1093
AcuI	CTGAAG	4 cut(s)	1025, 1482, 1489, 1638
AcvI	CACGTG	1 cut(s)	596
AcyI	GRCGYC	2 cut(s)	29, 35
AfaI	GTAC	2 cut(s)	965, 1191
AfiI	CCNNNNNNNGG	4 cut(s)	150, 1015, 1423, 2062
AflII	CTTAAG	1 cut(s)	41
AhdI	GACNNNNNGTC	1 cut(s)	1771
AhlI	ACTAGT	2 cut(s)	1169, 1945
AjiI	CACGTC	1 cut(s)	1921
AjnI	CCWGG	3 cut(s)	1030, 1108, 1927
AleI	CACNNNNGTG	2 cut(s)	702, 1552
AluBI	AGCT	8 cut(s)	838, 1063, 1270, 1307, 1336, 1433, 1552, 1978
AluI	AGCT	8 cut(s)	838, 1063, 1270, 1307, 1336, 1433, 1552, 1978
Alw21I	GWGCWC	4 cut(s)	138, 840, 1082, 1559
Alw26I	GTCTC	4 cut(s)	620, 893, 1448, 2024
Alw44I	GTGCAC	3 cut(s)	134, 1078, 1555
AlwI	GGATC	6 cut(s)	224, 282, 922, 935, 1748, 1761
AoxI	GGCC	6 cut(s)	121, 322, 781, 1006, 1274, 1745
ApaLI	GTGCAC	3 cut(s)	134, 1078, 1555
ApeKI	GCWGC	8 cut(s)	62, 203, 281, 515, 536, 620, 1060, 1975
ApoI	RAATTY	4 cut(s)	177, 436, 991, 1093
Asp700I	GAANNNNTTC	1 cut(s)	713
Asp718I	GGTACC	1 cut(s)	1189
AspLEI	GCGC	3 cut(s)	31, 37, 1975
AspS9I	GGNCC	2 cut(s)	1007, 1207
AsuHPI	GGTGA	5 cut(s)	173, 963, 1030, 1714, 2077
AvaII	GGWCC	1 cut(s)	1207
BaeGI	GKGCMC	4 cut(s)	138, 790, 1082, 1559
BamHI	GGATCC	2 cut(s)	927, 1753
BanI	GGYRCC	4 cut(s)	28, 34, 104, 1189
BanII	GRGCYC	1 cut(s)	840
BauI	CACGAG	1 cut(s)	1337
BbrPI	CACGTG	1 cut(s)	596
BbsI	GAAGAC	5 cut(s)	314, 609, 1031, 1534, 1644
Bbv12I	GWGCWC	4 cut(s)	138, 840, 1082, 1559
BbvCI	CCTCAGC	2 cut(s)	1271, 1449
BbvI	GCAGC	8 cut(s)	74, 190, 268, 502, 523, 607, 1072, 1987
BceAI	ACGGC	1 cut(s)	768
BcgI	CGANNNNNNTGC	4 cut(s)	100, 134, 489, 523
BciT130I	CCWGG	3 cut(s)	1032, 1110, 1929
BciVI	GTATCC	1 cut(s)	1711
BclI	TGATCA	1 cut(s)	87
BcoDI	GTCTC	4 cut(s)	620, 893, 1448, 2024
BcuI	ACTAGT	2 cut(s)	1169, 1945
BfaI	CTAG	6 cut(s)	101, 1133, 1170, 1292, 1596, 1946
BfmI	CTRYAG	1 cut(s)	60
BfoI	RGCGCY	2 cut(s)	32, 38
BfrI	CTTAAG	1 cut(s)	41
BfuAI	ACCTGC	1 cut(s)	1195
BfuI	GTATCC	1 cut(s)	1711
Bme1390I	CCNGG	3 cut(s)	1032, 1110, 1929
Bme18I	GGWCC	1 cut(s)	1207
BmeRI	GACNNNNNGTC	1 cut(s)	1771
BmgBI	CACGTC	1 cut(s)	1921
BmgT120I	GGNCC	2 cut(s)	1007, 1207
BmiI	GGNNCC	7 cut(s)	30, 36, 57, 106, 929, 1191, 1755
BmrFI	CCNGG	3 cut(s)	1032, 1110, 1929
BmrI	ACTGGG	1 cut(s)	1339
BmsI	GCATC	3 cut(s)	74, 293, 619
BmuI	ACTGGG	1 cut(s)	1339
BpiI	GAAGAC	5 cut(s)	314, 609, 1031, 1534, 1644
BpmI	CTGGAG	3 cut(s)	603, 1692, 1950
Bpu10I	CCTNAGC	3 cut(s)	834, 1271, 1449
BpuEI	CTTGAG	3 cut(s)	323, 1646, 2006
BsaAI	YACGTR	1 cut(s)	596
BsaBI	GATNNNNATC	2 cut(s)	89, 471
BsaHI	GRCGYC	2 cut(s)	29, 35
BsaI	GGTCTC	3 cut(s)	620, 1448, 2024
BsaJI	CCNNGG	4 cut(s)	529, 692, 1030, 1821
Bsc4I	CCNNNNNNNGG	4 cut(s)	150, 1015, 1423, 2062
Bse118I	RCCGGY	6 cut(s)	25, 31, 107, 518, 560, 606
Bse1I	ACTGG	2 cut(s)	586, 1345
Bse3DI	GCAATG	3 cut(s)	118, 940, 1682
Bse8I	GATNNNNATC	2 cut(s)	89, 471
BseBI	CCWGG	3 cut(s)	1032, 1110, 1929
BseDI	CCNNGG	4 cut(s)	529, 692, 1030, 1821
BseGI	GGATG	6 cut(s)	284, 699, 804, 829, 1666, 2002
BseJI	GATNNNNATC	2 cut(s)	89, 471
BseLI	CCNNNNNNNGG	4 cut(s)	150, 1015, 1423, 2062
BseMI	GCAATG	3 cut(s)	118, 940, 1682
BseMII	CTCAG	6 cut(s)	615, 758, 825, 1262, 1440, 1845
BseNI	ACTGG	2 cut(s)	586, 1345
BseRI	GAGGAG	4 cut(s)	242, 791, 1317, 1466
BseSI	GKGCMC	4 cut(s)	138, 790, 1082, 1559
BseX3I	CGGCCG	1 cut(s)	121
BseXI	GCAGC	8 cut(s)	74, 190, 268, 502, 523, 607, 1072, 1987
Bsh1236I	CGCG	1 cut(s)	1235
Bsh1285I	CGRYCG	1 cut(s)	124
BshFI	GGCC	6 cut(s)	123, 324, 783, 1008, 1276, 1747
BshNI	GGYRCC	4 cut(s)	28, 34, 104, 1189
BsiEI	CGRYCG	1 cut(s)	124
BsiHKAI	GWGCWC	4 cut(s)	138, 840, 1082, 1559
BsiSI	CCGG	6 cut(s)	26, 32, 108, 519, 561, 607
BslFI	GGGAC	3 cut(s)	27, 1141, 1802
BslI	CCNNNNNNNGG	4 cut(s)	150, 1015, 1423, 2062
BsmAI	GTCTC	4 cut(s)	620, 893, 1448, 2024
BsmFI	GGGAC	3 cut(s)	27, 1141, 1802
BsmI	GAATGC	1 cut(s)	495
BsnI	GGCC	6 cut(s)	123, 324, 783, 1008, 1276, 1747
Bso31I	GGTCTC	3 cut(s)	620, 1448, 2024
Bsp1286I	GDGCHC	5 cut(s)	138, 790, 840, 1082, 1559
Bsp143I	GATC	7 cut(s)	87, 229, 274, 927, 1531, 1696, 1753
BspANI	GGCC	6 cut(s)	123, 324, 783, 1008, 1276, 1747
BspCNI	CTCAG	6 cut(s)	616, 757, 826, 1263, 1441, 1846
BspFNI	CGCG	1 cut(s)	1235
BspLI	GGNNCC	7 cut(s)	30, 36, 57, 106, 929, 1191, 1755
BspMAI	CTGCAG	1 cut(s)	64
BspMI	ACCTGC	1 cut(s)	1195
BspPI	GGATC	6 cut(s)	224, 282, 922, 935, 1748, 1761
BspT107I	GGYRCC	4 cut(s)	28, 34, 104, 1189
BspTI	CTTAAG	1 cut(s)	41
BspTNI	GGTCTC	3 cut(s)	620, 1448, 2024
BsrBI	CCGCTC	2 cut(s)	126, 1992
BsrDI	GCAATG	3 cut(s)	118, 940, 1682
BsrFI	RCCGGY	6 cut(s)	25, 31, 107, 518, 560, 606
BsrI	ACTGG	2 cut(s)	586, 1345
BssAI	RCCGGY	6 cut(s)	25, 31, 107, 518, 560, 606
BssECI	CCNNGG	4 cut(s)	529, 692, 1030, 1821
BssMI	GATC	7 cut(s)	87, 229, 274, 927, 1531, 1696, 1753
BssNAI	GTATAC	1 cut(s)	895
BssNI	GRCGYC	2 cut(s)	29, 35
BssSI	CACGAG	1 cut(s)	1337
BssT1I	CCWWGG	2 cut(s)	529, 1821
Bst1107I	GTATAC	1 cut(s)	895
Bst2BI	CACGAG	1 cut(s)	1337
Bst2UI	CCWGG	3 cut(s)	1032, 1110, 1929
Bst4CI	ACNGT	7 cut(s)	160, 481, 636, 674, 1053, 1477, 1609
BstACI	GRCGYC	2 cut(s)	29, 35
BstAFI	CTTAAG	1 cut(s)	41
BstBAI	YACGTR	1 cut(s)	596
BstC8I	GCNNGC	6 cut(s)	27, 33, 109, 134, 1027, 1446
BstDEI	CTNAG	6 cut(s)	624, 744, 834, 1271, 1449, 1854
BstF5I	GGATG	6 cut(s)	284, 699, 804, 829, 1666, 2002
BstFNI	CGCG	1 cut(s)	1235
BstH2I	RGCGCY	2 cut(s)	32, 38
BstHHI	GCGC	3 cut(s)	31, 37, 1975
BstKTI	GATC	7 cut(s)	90, 232, 277, 930, 1534, 1699, 1756
BstMAI	GTCTC	4 cut(s)	620, 893, 1448, 2024
BstMBI	GATC	7 cut(s)	87, 229, 274, 927, 1531, 1696, 1753
BstMCI	CGRYCG	1 cut(s)	124
BstMWI	GCNNNNNNNGC	3 cut(s)	43, 62, 129
BstNI	CCWGG	3 cut(s)	1032, 1110, 1929
BstNSI	RCATGY	1 cut(s)	1204
BstSCI	CCNGG	3 cut(s)	1030, 1108, 1927
BstSFI	CTRYAG	1 cut(s)	60
BstSLI	GKGCMC	4 cut(s)	138, 790, 1082, 1559
BstUI	CGCG	1 cut(s)	1235
BstV1I	GCAGC	8 cut(s)	74, 190, 268, 502, 523, 607, 1072, 1987
BstV2I	GAAGAC	5 cut(s)	314, 609, 1031, 1534, 1644
BstX2I	RGATCY	2 cut(s)	927, 1753
BstXI	CCANNNNNNTGG	1 cut(s)	547
BstYI	RGATCY	2 cut(s)	927, 1753
BstZ17I	GTATAC	1 cut(s)	895
BstZI	CGGCCG	1 cut(s)	121
BsuI	GTATCC	1 cut(s)	1711
BsuRI	GGCC	6 cut(s)	123, 324, 783, 1008, 1276, 1747
BtrI	CACGTC	1 cut(s)	1921
BtsCI	GGATG	6 cut(s)	284, 699, 804, 829, 1666, 2002
BtsI	GCAGTG	1 cut(s)	1736
BtsIMutI	CAGTG	7 cut(s)	15, 632, 717, 1080, 1605, 1662, 1736
BveI	ACCTGC	1 cut(s)	1195
Cac8I	GCNNGC	6 cut(s)	27, 33, 109, 134, 1027, 1446
CfoI	GCGC	3 cut(s)	31, 37, 1975
Cfr10I	RCCGGY	6 cut(s)	25, 31, 107, 518, 560, 606
Cfr13I	GGNCC	2 cut(s)	1007, 1207
Csp6I	GTAC	2 cut(s)	964, 1190
CviQI	GTAC	2 cut(s)	964, 1190
DdeI	CTNAG	6 cut(s)	624, 744, 834, 1271, 1449, 1854
DinI	GGCGCC	2 cut(s)	30, 36
DpnI	GATC	7 cut(s)	89, 231, 276, 929, 1533, 1698, 1755
DpnII	GATC	7 cut(s)	87, 229, 274, 927, 1531, 1696, 1753
DriI	GACNNNNNGTC	1 cut(s)	1771
EaeI	YGGCCR	2 cut(s)	121, 781
EagI	CGGCCG	1 cut(s)	121
Eam1105I	GACNNNNNGTC	1 cut(s)	1771
EciI	GGCGGA	1 cut(s)	1216
Ecl136II	GAGCTC	1 cut(s)	838
EclXI	CGGCCG	1 cut(s)	121
Eco130I	CCWWGG	2 cut(s)	529, 1821
Eco147I	AGGCCT	1 cut(s)	1276
Eco24I	GRGCYC	1 cut(s)	840
Eco31I	GGTCTC	3 cut(s)	620, 1448, 2024
Eco47I	GGWCC	1 cut(s)	1207
Eco52I	CGGCCG	1 cut(s)	121
Eco53kI	GAGCTC	1 cut(s)	838
Eco57I	CTGAAG	4 cut(s)	1025, 1482, 1489, 1638
Eco72I	CACGTG	1 cut(s)	596
EcoICRI	GAGCTC	1 cut(s)	838
EcoRI	GAATTC	1 cut(s)	177
EcoRII	CCWGG	3 cut(s)	1030, 1108, 1927
EcoT14I	CCWWGG	2 cut(s)	529, 1821
EcoT22I	ATGCAT	1 cut(s)	656
EcoT38I	GRGCYC	1 cut(s)	840
EgeI	GGCGCC	2 cut(s)	30, 36
EheI	GGCGCC	2 cut(s)	30, 36
ErhI	CCWWGG	2 cut(s)	529, 1821
FaqI	GGGAC	3 cut(s)	27, 1141, 1802
FauI	CCCGC	3 cut(s)	42, 193, 1985
FauNDI	CATATG	2 cut(s)	772, 1591
FbaI	TGATCA	1 cut(s)	87
FblI	GTMKAC	1 cut(s)	894
FokI	GGATG	6 cut(s)	271, 686, 791, 816, 1673, 2009
FriOI	GRGCYC	1 cut(s)	840
FspBI	CTAG	6 cut(s)	101, 1133, 1170, 1292, 1596, 1946
GlaI	GCGC	3 cut(s)	30, 36, 1974
GsuI	CTGGAG	3 cut(s)	603, 1692, 1950
HaeII	RGCGCY	2 cut(s)	32, 38
HaeIII	GGCC	6 cut(s)	123, 324, 783, 1008, 1276, 1747
HapII	CCGG	6 cut(s)	26, 32, 108, 519, 561, 607
HhaI	GCGC	3 cut(s)	31, 37, 1975
Hin1I	GRCGYC	2 cut(s)	29, 35
Hin6I	GCGC	3 cut(s)	29, 35, 1973
HinP1I	GCGC	3 cut(s)	29, 35, 1973
HpaII	CCGG	6 cut(s)	26, 32, 108, 519, 561, 607
HphI	GGTGA	5 cut(s)	173, 963, 1030, 1714, 2077
Hpy166II	GTNNAC	8 cut(s)	136, 237, 717, 895, 1049, 1080, 1181, 1557
Hpy188I	TCNGA	7 cut(s)	477, 1044, 1248, 1528, 1753, 1777, 1840
Hpy188III	TCNNGA	9 cut(s)	69, 302, 760, 833, 1339, 1372, 1469, 1671, 1811
Hpy8I	GTNNAC	8 cut(s)	136, 237, 717, 895, 1049, 1080, 1181, 1557
Hpy99I	CGWCG	2 cut(s)	1922, 2052
HpyAV	CCTTC	7 cut(s)	154, 1118, 1314, 1434, 1758, 1867, 2009
HpyCH4III	ACNGT	7 cut(s)	160, 481, 636, 674, 1053, 1477, 1609
HpyCH4IV	ACGT	3 cut(s)	595, 1920, 2090
HpyF10VI	GCNNNNNNNGC	3 cut(s)	43, 62, 129
HpyF3I	CTNAG	6 cut(s)	624, 744, 834, 1271, 1449, 1854
HpySE526I	ACGT	3 cut(s)	595, 1920, 2090
Hsp92I	GRCGYC	2 cut(s)	29, 35
HspAI	GCGC	3 cut(s)	29, 35, 1973
KasI	GGCGCC	2 cut(s)	28, 34
KpnI	GGTACC	1 cut(s)	1193
KroI	GCCGGC	3 cut(s)	25, 31, 107
KroNI	GCCGGC	3 cut(s)	27, 33, 109
Ksp22I	TGATCA	1 cut(s)	87
Kzo9I	GATC	7 cut(s)	87, 229, 274, 927, 1531, 1696, 1753
LmnI	GCTCC	3 cut(s)	61, 843, 1304
Lsp1109I	GCAGC	8 cut(s)	74, 190, 268, 502, 523, 607, 1072, 1987
LweI	GCATC	3 cut(s)	74, 293, 619
MaeI	CTAG	6 cut(s)	101, 1133, 1170, 1292, 1596, 1946
MaeII	ACGT	3 cut(s)	595, 1920, 2090
MaeIII	GTNAC	6 cut(s)	256, 1165, 1339, 1567, 1702, 1763
MalI	GATC	7 cut(s)	89, 231, 276, 929, 1533, 1698, 1755
MbiI	CCGCTC	2 cut(s)	126, 1992
MboI	GATC	7 cut(s)	87, 229, 274, 927, 1531, 1696, 1753
MfeI	CAATTG	1 cut(s)	240
MflI	RGATCY	2 cut(s)	927, 1753
MhlI	GDGCHC	5 cut(s)	138, 790, 840, 1082, 1559
MluCI	AATT	8 cut(s)	177, 240, 436, 453, 991, 1093, 1384, 1400
Mly113I	GGCGCC	2 cut(s)	29, 35
MlyI	GAGTC	6 cut(s)	579, 1123, 1176, 1232, 1658, 1772
Mph1103I	ATGCAT	1 cut(s)	656
MreI	CGCCGGCG	2 cut(s)	25, 31
MroNI	GCCGGC	3 cut(s)	25, 31, 107
MroXI	GAANNNNTTC	1 cut(s)	713
MseI	TTAA	3 cut(s)	42, 960, 1410
MslI	CAYNNNNRTG	4 cut(s)	545, 615, 702, 1552
MspA1I	CMGCKG	2 cut(s)	1482, 1552
MspCI	CTTAAG	1 cut(s)	41
MspI	CCGG	6 cut(s)	26, 32, 108, 519, 561, 607
MspR9I	CCNGG	3 cut(s)	1032, 1110, 1929
MunI	CAATTG	1 cut(s)	240
Mva1269I	GAATGC	1 cut(s)	495
MvaI	CCWGG	3 cut(s)	1032, 1110, 1929
MvnI	CGCG	1 cut(s)	1235
MwoI	GCNNNNNNNGC	3 cut(s)	43, 62, 129
NaeI	GCCGGC	3 cut(s)	27, 33, 109
NarI	GGCGCC	2 cut(s)	29, 35
NdeI	CATATG	2 cut(s)	772, 1591
NdeII	GATC	7 cut(s)	87, 229, 274, 927, 1531, 1696, 1753
NgoMIV	GCCGGC	3 cut(s)	25, 31, 107
NlaIV	GGNNCC	7 cut(s)	30, 36, 57, 106, 929, 1191, 1755
NmeAIII	GCCGAG	2 cut(s)	350, 673
NmuCI	GTSAC	4 cut(s)	1339, 1567, 1702, 1763
NsiI	ATGCAT	1 cut(s)	656
NspI	RCATGY	1 cut(s)	1204
OliI	CACNNNNGTG	2 cut(s)	702, 1552
PceI	AGGCCT	1 cut(s)	1276
PcsI	WCGNNNNNNNCGW	1 cut(s)	700
PctI	GAATGC	1 cut(s)	495
PdiI	GCCGGC	3 cut(s)	27, 33, 109
PdmI	GAANNNNTTC	1 cut(s)	713
PfeI	GAWTC	5 cut(s)	662, 763, 1667, 1835, 2052
PflMI	CCANNNNNTGG	1 cut(s)	1423
PleI	GAGTC	6 cut(s)	578, 1123, 1176, 1232, 1657, 1772
PluTI	GGCGCC	2 cut(s)	32, 38
PmaCI	CACGTG	1 cut(s)	596
PmlI	CACGTG	1 cut(s)	596
PpsI	GAGTC	6 cut(s)	578, 1123, 1176, 1232, 1657, 1772
Ppu21I	YACGTR	1 cut(s)	596
Psp124BI	GAGCTC	1 cut(s)	840
Psp6I	CCWGG	3 cut(s)	1030, 1108, 1927
PspCI	CACGTG	1 cut(s)	596
PspGI	CCWGG	3 cut(s)	1030, 1108, 1927
PspN4I	GGNNCC	7 cut(s)	30, 36, 57, 106, 929, 1191, 1755
PspPI	GGNCC	2 cut(s)	1007, 1207
PstI	CTGCAG	1 cut(s)	64
PsuI	RGATCY	2 cut(s)	927, 1753
PvuII	CAGCTG	1 cut(s)	1552
RsaI	GTAC	2 cut(s)	965, 1191
RsaNI	GTAC	2 cut(s)	964, 1190
RseI	CAYNNNNRTG	4 cut(s)	545, 615, 702, 1552
SacI	GAGCTC	1 cut(s)	840
SaqAI	TTAA	3 cut(s)	42, 960, 1410
Sau3AI	GATC	7 cut(s)	87, 229, 274, 927, 1531, 1696, 1753
Sau96I	GGNCC	2 cut(s)	1007, 1207
SchI	GAGTC	6 cut(s)	579, 1123, 1176, 1232, 1658, 1772
ScrFI	CCNGG	3 cut(s)	1032, 1110, 1929
SduI	GDGCHC	5 cut(s)	138, 790, 840, 1082, 1559
SfaNI	GCATC	3 cut(s)	74, 293, 619
SfcI	CTRYAG	1 cut(s)	60
SfoI	GGCGCC	2 cut(s)	30, 36
SgrAI	CRCCGGYG	2 cut(s)	25, 31
SinI	GGWCC	1 cut(s)	1207
SmiMI	CAYNNNNRTG	4 cut(s)	545, 615, 702, 1552
SmlI	CTYRAG	4 cut(s)	41, 302, 1625, 2021
SmoI	CTYRAG	4 cut(s)	41, 302, 1625, 2021
SpeI	ACTAGT	2 cut(s)	1169, 1945
Sse9I	AATT	8 cut(s)	177, 240, 436, 453, 991, 1093, 1384, 1400
SseBI	AGGCCT	1 cut(s)	1276
SspDI	GGCGCC	2 cut(s)	28, 34
SspI	AATATT	1 cut(s)	847
SspMI	CTAG	6 cut(s)	101, 1133, 1170, 1292, 1596, 1946
SstI	GAGCTC	1 cut(s)	840
StuI	AGGCCT	1 cut(s)	1276
StyD4I	CCNGG	3 cut(s)	1030, 1108, 1927
StyI	CCWWGG	2 cut(s)	529, 1821
TaaI	ACNGT	7 cut(s)	160, 481, 636, 674, 1053, 1477, 1609
TaiI	ACGT	3 cut(s)	598, 1923, 2093
TaqI	TCGA	4 cut(s)	499, 665, 1810, 2050
TasI	AATT	8 cut(s)	177, 240, 436, 453, 991, 1093, 1384, 1400
TatI	WGTACW	1 cut(s)	963
TauI	GCSGC	2 cut(s)	49, 126
TfiI	GAWTC	5 cut(s)	662, 763, 1667, 1835, 2052
Tru1I	TTAA	3 cut(s)	42, 960, 1410
Tru9I	TTAA	3 cut(s)	42, 960, 1410
TscAI	CASTG	7 cut(s)	15, 639, 724, 1087, 1612, 1662, 1743
TseFI	GTSAC	4 cut(s)	1339, 1567, 1702, 1763
TseI	GCWGC	8 cut(s)	62, 203, 281, 515, 536, 620, 1060, 1975
Tsp45I	GTSAC	4 cut(s)	1339, 1567, 1702, 1763
TspDTI	ATGAA	6 cut(s)	129, 932, 943, 1167, 1209, 1286
TspGWI	ACGGA	5 cut(s)	222, 389, 605, 692, 1591
TspRI	CASTG	7 cut(s)	15, 639, 724, 1087, 1612, 1662, 1743
Van91I	CCANNNNNTGG	1 cut(s)	1423
Vha464I	CTTAAG	1 cut(s)	41
VneI	GTGCAC	3 cut(s)	134, 1078, 1555
VpaK11BI	GGWCC	1 cut(s)	1207
XapI	RAATTY	4 cut(s)	177, 436, 991, 1093
XceI	RCATGY	1 cut(s)	1204
XmiI	GTMKAC	1 cut(s)	894
XmnI	GAANNNNTTC	1 cut(s)	713
XspI	CTAG	6 cut(s)	101, 1133, 1170, 1292, 1596, 1946
Zsp2I	ATGCAT	1 cut(s)	656

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.