RLG00000002186

Cleavage and polyadenylation specificity factor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0002312
Physical Location & Seq
Forward (+)
26919974 .. 26922871
2898 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002186

Sequence Viewer

Length: 2079 bp
ATGGGTTCGACAGGGGGAGTAAAGAGGCGCGAGGCATCATCGTCTTCGGCGGCAAAAGAGGAAGATAAGCTGATTGTGACTCCATTAGGAGCCGGCAATGAAGTTGGTCGCTCTTGCGTTTACATGTCCTACAAGGGCAAAACTGTCTTGTTTGATTGCGGCATCCATCCCGCTTACTCCGGCATGGCTGCTCTCCCTTACTTTGATGAGATTGATCCTTCCACTATTGATGTCCTTCTCATTACCCATTTTCACTTGGATCATGCTGCATCCCTGCCTTACTTCCTTGAGAAGACCACATTCAAAGGCCGAGTCTTTATGACTTATGCAACCAAGGCTATCTACAAGTTGCTTTTGACAGACTATGTGAAAGTCAGCAAAGTTTCAGTGGAAGATATGTTGTTCGATGAGCAGGACATAAATGCTTCTATGGATAAAATTGAGGTTATTGATTTCCACCAAACAGTAGAAGTTGAGGGCATTCGGTTTTGGTGCTACACTGCAGGTCATGTCCTTGGTGCTGCTATGTTTATGGTTGATATTGCTGGTGTTAGGGTCCTCTACACTGGAGATTATTCACGTGAAGAAGACCGACACCTCCGTGCTGCTGAGACCCCACAGTTCTCCCCCGATATATGCATTATTGAGTCCACATATGGTGTCCAGCACCATCAACCTCGAAACATCCGTGAGAAGCGATTCACTGAAGCTATACACACTGCCCTCTCTCGAGGGGGTCGTGTCTTAATTCCTGCATTTGCACTTGGCCGTGCCCAAGAACTTCTCCTGATCCTTGATGAGTATTGGGCAAATCATCCAGAGCTCCAAAATATACCTATATATTATGCTTCCCCACTGGCAAAAAGATGTCTGTCTGTTTATGAGACATACACCCTTTCAATGAATGATAGGATCCGCAATGCGAAGTCAAATCCCTTCATCTTCAAGTACATATCACCACTAAAGAGCATTGAGAATTTCAAAGATGTAGGCCCATCGGTCGTGATGGCAAGCCCTGGTGGTCTTCAAAGCGGGCTGTCAAGGCAGCTATTTGATATGTGGTGCTCTGATAAGAGAAATGCATGTGTGATTCCGGGTTATGTGGTTGAGGGGACACTTGCTAAGACAATCATCAATGAACCGAAAGAGGTTACCCTTATGAATGGGTTAGCTGCTCCGCTCAACATGTCGGTCCATTACATTTCATTCTCTGCTCATGCAGATTCTGTTCAGACAAGTGCATTTTTGGAGGAGCTGAGGCCTCCTAACATAATCCTTGTTCATGGAGAAGCTCATGAGATGGGAAGGCTGAAAGAGAAGCTCATGACTCAGTTTCGTGATCGCAACACAAAAATCCTCACACCAAAGAATTGTCAGTCTGTTGAAATGTATTTTAACTCACAAAAAATGGCTAAAGCTATTGGAAGGCTTGCTGAGAGGACCCCGGAAGTTGGTGAAAAAGTCAGCGGTTTACTGGTAAAAAAAGGTTTCAGTTATCAGATAATGGCTTCTGAAGATCTTCATGTTTTTTCACAGCTGTCCACGGCAAATGTCACTCAACGTATTACTATCCCTTATACTACTGGCTTCATGGTGATAAAACATCGGCTGAGGCAAATATATGAAAGTGTAGAATCTTCTGTGGATGAGGAATCTGGAGTGCCGACATTGCAAGTGCATGATCGAGTGACTGTGAGGCAGGATACAGATAAACATGTTTCACTGCATTGGATGTCAGATCCTATAAGTGACATGGTGTCAGACTCTATCGTAGCTCTAATTTTGAATATCAACAGAGAAGTCCCCAAGGTAGTCATCGAATCAGAGGACATTAAGACAGAGGAAGAGAATGGCAGGAAAACAGAAAAGGTCATTCATGCACTTCTTGTATCACTCTTTGGGGATGTGAAGCCTGGAGAAAATGGGAAGTTGGTGATCAGTGTTGATGGAAATGTGGCACAACTTGATAAACAGAGTGGGGACGTAGAGAGTGAAAATGAAGGCCTCAAGGAAAGAGTAAAGACAGCATTTCGTCGAATCCAAAGTGCAGTGAAGCCAATTTCTCTCTCTGCATCTTAG

Protein Analysis

693

Amino Acids

77.38

Weight (kDa)

6.19

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B PF00753 35 - 198 5.7e-20 Metallo-beta-lactamase superfamily
Anti-Pycsar_Apyc1 PF23023 42 - 198 9.1e-09 Anti-Pycsar protein Apyc1
Lactamase_B_6 PF16661 44 - 205 8.8e-18 Metallo-beta-lactamase superfamily domain
Lactamase_B_2 PF12706 48 - 198 4.6e-07 Beta-lactamase superfamily domain
Beta-Casp PF10996 258 - 377 9.3e-29 Beta-Casp domain
RMMBL PF07521 393 - 445 7.5e-13 Zn-dependent metallo-hydrolase RNA specificity domain
CPSF73-100_C PF11718 487 - 686 1.2e-51 Pre-mRNA 3'-end-processing endonuclease polyadenylation factor C-term
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 494
AccBSI CCGCTC 1 cut(s) 1180
AccII CGCG 1 cut(s) 30
AciI CCGC 7 cut(s) 50, 159, 171, 916, 1032, 1178, 1467
AclWI GGATC 6 cut(s) 209, 267, 784, 907, 920, 1733
AcoI YGGCCR 1 cut(s) 766
AcsI RAATTY 1 cut(s) 976
AcuI CTGAAG 2 cut(s) 726, 1533
AcvI CACGTG 1 cut(s) 581
AfaI GTAC 1 cut(s) 950
AfiI CCNNNNNNNGG 1 cut(s) 1451
AflIII ACRYGT 3 cut(s) 123, 1185, 1714
AgsI TTSAA 7 cut(s) 304, 900, 946, 982, 1028, 1385, 1786
AhdI GACNNNNNGTC 1 cut(s) 1756
AjnI CCWGG 2 cut(s) 1015, 1912
AleI CACNNNNGTG 1 cut(s) 600
Alw21I GWGCWC 2 cut(s) 825, 1067
Alw26I GTCTC 2 cut(s) 605, 878
AlwI GGATC 6 cut(s) 209, 267, 784, 907, 920, 1733
AlwNI CAGNNNCTG 1 cut(s) 1226
Ama87I CYCGRG 1 cut(s) 729
AoxI GGCC 5 cut(s) 307, 766, 991, 1259, 2002
ApeKI GCWGC 6 cut(s) 188, 266, 521, 605, 1045, 1172
ApoI RAATTY 1 cut(s) 976
ArsI GACNNNNNNTTYG 2 cut(s) 297, 329
Asp700I GAANNNNTTC 2 cut(s) 698, 1518
AspLEI GCGC 1 cut(s) 30
AspS9I GGNCC 4 cut(s) 556, 992, 1192, 1440
AsuC2I CCSGG 2 cut(s) 1095, 1445
AsuHPI GGTGA 4 cut(s) 948, 1466, 1606, 1945
AvaI CYCGRG 1 cut(s) 729
AvaII GGWCC 3 cut(s) 556, 1192, 1440
BaeGI GKGCMC 1 cut(s) 775
BamHI GGATCC 1 cut(s) 912
BanII GRGCYC 1 cut(s) 825
BbrPI CACGTG 1 cut(s) 581
BbsI GAAGAC 4 cut(s) 36, 299, 594, 1016
Bbv12I GWGCWC 2 cut(s) 825, 1067
BbvCI CCTCAGC 2 cut(s) 1256, 1610
BbvI GCAGC 6 cut(s) 175, 253, 508, 592, 1057, 1159
BccI CCATC 6 cut(s) 174, 678, 1000, 1003, 1294, 1940
BceAI ACGGC 2 cut(s) 753, 1560
BciT130I CCWGG 2 cut(s) 1017, 1914
BciVI GTATCC 1 cut(s) 1696
BclI TGATCA 1 cut(s) 1935
BcnI CCSGG 2 cut(s) 1095, 1445
BcoDI GTCTC 2 cut(s) 605, 878
BfmI CTRYAG 1 cut(s) 501
BfuAI ACCTGC 1 cut(s) 494
BfuI GTATCC 1 cut(s) 1696
BglII AGATCT 1 cut(s) 1516
BisI GCNGC 8 cut(s) 51, 160, 189, 267, 522, 606, 1046, 1173
BlsI GCNGC 8 cut(s) 52, 161, 190, 268, 523, 607, 1047, 1174
Bme1390I CCNGG 4 cut(s) 1017, 1095, 1445, 1914
Bme18I GGWCC 3 cut(s) 556, 1192, 1440
BmeRI GACNNNNNGTC 1 cut(s) 1756
BmeT110I CYCGRG 1 cut(s) 729
BmgT120I GGNCC 4 cut(s) 556, 992, 1192, 1440
BmiI GGNNCC 4 cut(s) 91, 557, 914, 1442
BmrFI CCNGG 4 cut(s) 1017, 1095, 1445, 1914
BmsI GCATC 3 cut(s) 44, 171, 278
BpiI GAAGAC 4 cut(s) 36, 299, 594, 1016
BpmI CTGGAG 3 cut(s) 588, 1677, 1935
Bpu10I CCTNAGC 2 cut(s) 1256, 1610
BpuEI CTTGAG 2 cut(s) 308, 1991
BpuMI CCSGG 2 cut(s) 1095, 1445
BsaAI YACGTR 1 cut(s) 581
BsaI GGTCTC 1 cut(s) 605
BsaJI CCNNGG 6 cut(s) 333, 514, 1015, 1443, 1542, 1806
Bsc4I CCNNNNNNNGG 1 cut(s) 1451
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 4 cut(s) 571, 861, 1479, 1588
Bse3DI GCAATG 3 cut(s) 103, 925, 1667
BseBI CCWGG 2 cut(s) 1017, 1914
BseDI CCNNGG 6 cut(s) 333, 514, 1015, 1443, 1542, 1806
BseGI GGATG 8 cut(s) 162, 166, 269, 684, 814, 1651, 1737, 1909
BseLI CCNNNNNNNGG 1 cut(s) 1451
BseMI GCAATG 3 cut(s) 103, 925, 1667
BseMII CTCAG 5 cut(s) 600, 1247, 1343, 1425, 1601
BseNI ACTGG 4 cut(s) 571, 861, 1479, 1588
BseRI GAGGAG 1 cut(s) 1265
BseSI GKGCMC 1 cut(s) 775
BseXI GCAGC 6 cut(s) 175, 253, 508, 592, 1057, 1159
BsgI GTGCAG 1 cut(s) 2067
Bsh1236I CGCG 1 cut(s) 30
Bsh1285I CGRYCG 1 cut(s) 1002
BshFI GGCC 5 cut(s) 309, 768, 993, 1261, 2004
BsiEI CGRYCG 1 cut(s) 1002
BsiHKAI GWGCWC 2 cut(s) 825, 1067
BsiHKCI CYCGRG 1 cut(s) 729
BsiSI CCGG 4 cut(s) 93, 180, 1094, 1445
BslFI GGGAC 3 cut(s) 1126, 1787, 1994
BslI CCNNNNNNNGG 1 cut(s) 1451
BsmAI GTCTC 2 cut(s) 605, 878
BsmFI GGGAC 3 cut(s) 1126, 1787, 1994
BsmI GAATGC 1 cut(s) 480
BsnI GGCC 5 cut(s) 309, 768, 993, 1261, 2004
Bso31I GGTCTC 1 cut(s) 605
BsoBI CYCGRG 1 cut(s) 729
Bsp1286I GDGCHC 3 cut(s) 775, 825, 1067
Bsp143I GATC 9 cut(s) 214, 259, 789, 912, 1339, 1516, 1681, 1738, 1935
BspACI CCGC 7 cut(s) 50, 159, 171, 916, 1032, 1178, 1467
BspANI GGCC 5 cut(s) 309, 768, 993, 1261, 2004
BspCNI CTCAG 5 cut(s) 601, 1248, 1342, 1426, 1602
BspFNI CGCG 1 cut(s) 30
BspHI TCATGA 2 cut(s) 1294, 1323
BspLI GGNNCC 4 cut(s) 91, 557, 914, 1442
BspMAI CTGCAG 1 cut(s) 505
BspMI ACCTGC 1 cut(s) 494
BspPI GGATC 6 cut(s) 209, 267, 784, 907, 920, 1733
BspTNI GGTCTC 1 cut(s) 605
BsrBI CCGCTC 1 cut(s) 1180
BsrDI GCAATG 3 cut(s) 103, 925, 1667
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 4 cut(s) 571, 861, 1479, 1588
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 6 cut(s) 333, 514, 1015, 1443, 1542, 1806
BssMI GATC 9 cut(s) 214, 259, 789, 912, 1339, 1516, 1681, 1738, 1935
BssT1I CCWWGG 3 cut(s) 333, 514, 1806
Bst2UI CCWGG 2 cut(s) 1017, 1914
Bst4CI ACNGT 4 cut(s) 145, 466, 621, 1693
Bst6I CTCTTC 1 cut(s) 1839
BstBAI YACGTR 1 cut(s) 581
BstC8I GCNNGC 4 cut(s) 94, 1012, 1034, 1431
BstDEI CTNAG 7 cut(s) 609, 1122, 1256, 1329, 1434, 1610, 2076
BstDSI CCRYGG 1 cut(s) 1542
BstEII GGTNACC 1 cut(s) 1150
BstF5I GGATG 8 cut(s) 162, 166, 269, 684, 814, 1651, 1737, 1909
BstFNI CGCG 1 cut(s) 30
BstHHI GCGC 1 cut(s) 30
BstKTI GATC 9 cut(s) 217, 262, 792, 915, 1342, 1519, 1684, 1741, 1938
BstMAI GTCTC 2 cut(s) 605, 878
BstMBI GATC 9 cut(s) 214, 259, 789, 912, 1339, 1516, 1681, 1738, 1935
BstMCI CGRYCG 1 cut(s) 1002
BstMWI GCNNNNNNNGC 3 cut(s) 335, 1042, 1669
BstNI CCWGG 2 cut(s) 1017, 1914
BstNSI RCATGY 4 cut(s) 127, 1086, 1189, 1718
BstPI GGTNACC 1 cut(s) 1150
BstSCI CCNGG 4 cut(s) 1015, 1093, 1443, 1912
BstSFI CTRYAG 1 cut(s) 501
BstSLI GKGCMC 1 cut(s) 775
BstUI CGCG 1 cut(s) 30
BstV1I GCAGC 6 cut(s) 175, 253, 508, 592, 1057, 1159
BstV2I GAAGAC 4 cut(s) 36, 299, 594, 1016
BstX2I RGATCY 3 cut(s) 912, 1516, 1738
BstYI RGATCY 3 cut(s) 912, 1516, 1738
BsuI GTATCC 1 cut(s) 1696
BsuRI GGCC 5 cut(s) 309, 768, 993, 1261, 2004
BtgI CCRYGG 1 cut(s) 1542
BtsCI GGATG 8 cut(s) 162, 166, 269, 684, 814, 1651, 1737, 1909
BtsI GCAGTG 4 cut(s) 498, 717, 1721, 2055
BtsIMutI CAGTG 9 cut(s) 393, 498, 564, 702, 717, 854, 1721, 1945, 2055
BveI ACCTGC 1 cut(s) 494
Cac8I GCNNGC 4 cut(s) 94, 1012, 1034, 1431
CaiI CAGNNNCTG 1 cut(s) 1226
CciI TCATGA 2 cut(s) 1294, 1323
CfoI GCGC 1 cut(s) 30
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 4 cut(s) 556, 992, 1192, 1440
Csp6I GTAC 1 cut(s) 949
CviQI GTAC 1 cut(s) 949
DdeI CTNAG 7 cut(s) 609, 1122, 1256, 1329, 1434, 1610, 2076
DpnI GATC 9 cut(s) 216, 261, 791, 914, 1341, 1518, 1683, 1740, 1937
DpnII GATC 9 cut(s) 214, 259, 789, 912, 1339, 1516, 1681, 1738, 1935
DriI GACNNNNNGTC 1 cut(s) 1756
EaeI YGGCCR 1 cut(s) 766
Eam1104I CTCTTC 1 cut(s) 1839
Eam1105I GACNNNNNGTC 1 cut(s) 1756
EarI CTCTTC 1 cut(s) 1839
Ecl136II GAGCTC 1 cut(s) 823
Eco130I CCWWGG 3 cut(s) 333, 514, 1806
Eco147I AGGCCT 2 cut(s) 1261, 2004
Eco24I GRGCYC 1 cut(s) 825
Eco31I GGTCTC 1 cut(s) 605
Eco47I GGWCC 3 cut(s) 556, 1192, 1440
Eco53kI GAGCTC 1 cut(s) 823
Eco57I CTGAAG 2 cut(s) 726, 1533
Eco72I CACGTG 1 cut(s) 581
Eco88I CYCGRG 1 cut(s) 729
Eco91I GGTNACC 1 cut(s) 1150
EcoICRI GAGCTC 1 cut(s) 823
EcoO109I RGGNCCY 2 cut(s) 556, 1440
EcoO65I GGTNACC 1 cut(s) 1150
EcoRII CCWGG 2 cut(s) 1015, 1912
EcoT14I CCWWGG 3 cut(s) 333, 514, 1806
EcoT22I ATGCAT 2 cut(s) 641, 1084
EcoT38I GRGCYC 1 cut(s) 825
ErhI CCWWGG 3 cut(s) 333, 514, 1806
FaqI GGGAC 3 cut(s) 1126, 1787, 1994
FauI CCCGC 2 cut(s) 178, 1025
FauNDI CATATG 1 cut(s) 655
FbaI TGATCA 1 cut(s) 1935
Fnu4HI GCNGC 8 cut(s) 51, 160, 189, 267, 522, 606, 1046, 1173
FokI GGATG 8 cut(s) 149, 153, 256, 671, 801, 1658, 1744, 1916
FriOI GRGCYC 1 cut(s) 825
Fsp4HI GCNGC 8 cut(s) 51, 160, 189, 267, 522, 606, 1046, 1173
GlaI GCGC 1 cut(s) 29
GluI GCNGC 8 cut(s) 51, 160, 189, 267, 522, 606, 1046, 1173
GsuI CTGGAG 3 cut(s) 588, 1677, 1935
HaeIII GGCC 5 cut(s) 309, 768, 993, 1261, 2004
HapII CCGG 4 cut(s) 93, 180, 1094, 1445
HhaI GCGC 1 cut(s) 30
Hin6I GCGC 1 cut(s) 28
HinP1I GCGC 1 cut(s) 28
HpaII CCGG 4 cut(s) 93, 180, 1094, 1445
HphI GGTGA 4 cut(s) 948, 1466, 1606, 1945
Hpy166II GTNNAC 4 cut(s) 121, 651, 1472, 1542
Hpy188I TCNGA 7 cut(s) 1069, 1233, 1500, 1513, 1738, 1762, 1825
Hpy188III TCNNGA 8 cut(s) 729, 787, 818, 1003, 1295, 1324, 1337, 1656
Hpy8I GTNNAC 4 cut(s) 121, 651, 1472, 1542
Hpy99I CGWCG 1 cut(s) 2037
HpyAV CCTTC 6 cut(s) 228, 245, 946, 1299, 1419, 1994
HpyCH4III ACNGT 4 cut(s) 145, 466, 621, 1693
HpyCH4IV ACGT 3 cut(s) 580, 1561, 1983
HpyF10VI GCNNNNNNNGC 3 cut(s) 335, 1042, 1669
HpyF3I CTNAG 7 cut(s) 609, 1122, 1256, 1329, 1434, 1610, 2076
HpySE526I ACGT 3 cut(s) 580, 1561, 1983
HspAI GCGC 1 cut(s) 28
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
Ksp22I TGATCA 1 cut(s) 1935
Kzo9I GATC 9 cut(s) 214, 259, 789, 912, 1339, 1516, 1681, 1738, 1935
LmnI GCTCC 4 cut(s) 89, 828, 1180, 1252
Lsp1109I GCAGC 6 cut(s) 175, 253, 508, 592, 1057, 1159
LweI GCATC 3 cut(s) 44, 171, 278
MaeII ACGT 3 cut(s) 580, 1561, 1983
MaeIII GTNAC 5 cut(s) 76, 1150, 1552, 1687, 1748
MalI GATC 9 cut(s) 216, 261, 791, 914, 1341, 1518, 1683, 1740, 1937
MbiI CCGCTC 1 cut(s) 1180
MboI GATC 9 cut(s) 214, 259, 789, 912, 1339, 1516, 1681, 1738, 1935
MflI RGATCY 3 cut(s) 912, 1516, 1738
MhlI GDGCHC 3 cut(s) 775, 825, 1067
MluCI AATT 6 cut(s) 438, 747, 976, 1369, 1779, 2058
MlyI GAGTC 5 cut(s) 73, 321, 656, 1321, 1757
Mph1103I ATGCAT 2 cut(s) 641, 1084
MroNI GCCGGC 1 cut(s) 92
MroXI GAANNNNTTC 2 cut(s) 698, 1518
MseI TTAA 3 cut(s) 746, 1395, 1833
MslI CAYNNNNRTG 1 cut(s) 600
MspA1I CMGCKG 2 cut(s) 1467, 1537
MspI CCGG 4 cut(s) 93, 180, 1094, 1445
MspR9I CCNGG 4 cut(s) 1017, 1095, 1445, 1914
Mva1269I GAATGC 1 cut(s) 480
MvaI CCWGG 2 cut(s) 1017, 1914
MvnI CGCG 1 cut(s) 30
MwoI GCNNNNNNNGC 3 cut(s) 335, 1042, 1669
NaeI GCCGGC 1 cut(s) 94
NciI CCSGG 2 cut(s) 1095, 1445
NdeI CATATG 1 cut(s) 655
NdeII GATC 9 cut(s) 214, 259, 789, 912, 1339, 1516, 1681, 1738, 1935
NgoMIV GCCGGC 1 cut(s) 92
NlaIV GGNNCC 4 cut(s) 91, 557, 914, 1442
NmeAIII GCCGAG 1 cut(s) 335
NmuCI GTSAC 4 cut(s) 76, 1552, 1687, 1748
NsiI ATGCAT 2 cut(s) 641, 1084
NspI RCATGY 4 cut(s) 127, 1086, 1189, 1718
OliI CACNNNNGTG 1 cut(s) 600
PaeR7I CTCGAG 1 cut(s) 729
PagI TCATGA 2 cut(s) 1294, 1323
PceI AGGCCT 2 cut(s) 1261, 2004
PciI ACATGT 3 cut(s) 123, 1185, 1714
PcsI WCGNNNNNNNCGW 2 cut(s) 685, 736
PctI GAATGC 1 cut(s) 480
PdiI GCCGGC 1 cut(s) 94
PdmI GAANNNNTTC 2 cut(s) 698, 1518
PfeI GAWTC 7 cut(s) 699, 1090, 1223, 1634, 1652, 1820, 2037
PkrI GCNGC 8 cut(s) 52, 161, 190, 268, 523, 607, 1047, 1174
PleI GAGTC 5 cut(s) 73, 320, 655, 1321, 1757
PmaCI CACGTG 1 cut(s) 581
PmlI CACGTG 1 cut(s) 581
PpsI GAGTC 5 cut(s) 73, 320, 655, 1321, 1757
Ppu21I YACGTR 1 cut(s) 581
PpuMI RGGWCCY 2 cut(s) 556, 1440
PscI ACATGT 3 cut(s) 123, 1185, 1714
Psp124BI GAGCTC 1 cut(s) 825
Psp5II RGGWCCY 2 cut(s) 556, 1440
Psp6I CCWGG 2 cut(s) 1015, 1912
PspCI CACGTG 1 cut(s) 581
PspEI GGTNACC 1 cut(s) 1150
PspGI CCWGG 2 cut(s) 1015, 1912
PspN4I GGNNCC 4 cut(s) 91, 557, 914, 1442
PspPI GGNCC 4 cut(s) 556, 992, 1192, 1440
PspPPI RGGWCCY 2 cut(s) 556, 1440
PstI CTGCAG 1 cut(s) 505
PstNI CAGNNNCTG 1 cut(s) 1226
PsuI RGATCY 3 cut(s) 912, 1516, 1738
PvuII CAGCTG 1 cut(s) 1537
RsaI GTAC 1 cut(s) 950
RsaNI GTAC 1 cut(s) 949
RseI CAYNNNNRTG 1 cut(s) 600
SacI GAGCTC 1 cut(s) 825
SaqAI TTAA 3 cut(s) 746, 1395, 1833
SatI GCNGC 8 cut(s) 51, 160, 189, 267, 522, 606, 1046, 1173
Sau3AI GATC 9 cut(s) 214, 259, 789, 912, 1339, 1516, 1681, 1738, 1935
Sau96I GGNCC 4 cut(s) 556, 992, 1192, 1440
SchI GAGTC 5 cut(s) 73, 321, 656, 1321, 1757
ScrFI CCNGG 4 cut(s) 1017, 1095, 1445, 1914
SduI GDGCHC 3 cut(s) 775, 825, 1067
SfaNI GCATC 3 cut(s) 44, 171, 278
SfcI CTRYAG 1 cut(s) 501
Sfr274I CTCGAG 1 cut(s) 729
SinI GGWCC 3 cut(s) 556, 1192, 1440
SlaI CTCGAG 1 cut(s) 729
SmiMI CAYNNNNRTG 1 cut(s) 600
SmlI CTYRAG 3 cut(s) 287, 729, 2006
SmoI CTYRAG 3 cut(s) 287, 729, 2006
Sse9I AATT 6 cut(s) 438, 747, 976, 1369, 1779, 2058
SseBI AGGCCT 2 cut(s) 1261, 2004
SsiI CCGC 7 cut(s) 50, 159, 171, 916, 1032, 1178, 1467
SstI GAGCTC 1 cut(s) 825
StuI AGGCCT 2 cut(s) 1261, 2004
StyD4I CCNGG 4 cut(s) 1015, 1093, 1443, 1912
StyI CCWWGG 3 cut(s) 333, 514, 1806
TaaI ACNGT 4 cut(s) 145, 466, 621, 1693
TaiI ACGT 3 cut(s) 583, 1564, 1986
TaqI TCGA 7 cut(s) 8, 405, 679, 730, 1684, 1818, 2035
TaqII GACCGA 3 cut(s) 606, 988, 1180
TasI AATT 6 cut(s) 438, 747, 976, 1369, 1779, 2058
TatI WGTACW 1 cut(s) 948
TauI GCSGC 2 cut(s) 53, 162
TfiI GAWTC 7 cut(s) 699, 1090, 1223, 1634, 1652, 1820, 2037
Tru1I TTAA 3 cut(s) 746, 1395, 1833
Tru9I TTAA 3 cut(s) 746, 1395, 1833
TscAI CASTG 9 cut(s) 393, 505, 571, 709, 724, 861, 1728, 1945, 2055
TseFI GTSAC 4 cut(s) 76, 1552, 1687, 1748
TseI GCWGC 6 cut(s) 188, 266, 521, 605, 1045, 1172
Tsp45I GTSAC 4 cut(s) 76, 1552, 1687, 1748
TspGWI ACGGA 2 cut(s) 590, 677
TspRI CASTG 9 cut(s) 393, 505, 571, 709, 724, 861, 1728, 1945, 2055
VpaK11BI GGWCC 3 cut(s) 556, 1192, 1440
XapI RAATTY 1 cut(s) 976
XceI RCATGY 4 cut(s) 127, 1086, 1189, 1718
XhoI CTCGAG 1 cut(s) 729
XmnI GAANNNNTTC 2 cut(s) 698, 1518
Zsp2I ATGCAT 2 cut(s) 641, 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.