FvH4_6g13220

Glucose-induced degradation protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005099
Physical Location & Seq
Forward (+)
8051446 .. 8053945
2500 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g13220.t1

Sequence Viewer

Length: 648 bp
ATGCCGGTCAGGGCGGCAGAGACCTCCGCGCCTCCTCCACATCATTCAGGTTCCACCTGTGGACAGACATCTCCTCCACCCTTTACCCTTTTAAGCGTTGGCCAGGGCTTCTCGGGTACTCAGAATGTTTCGAGTCTGCAAAAAGATGAGGCTTGGAGAGTAAATGTTCGGATACAAGGGTGTGATCTTGAGAATGGTTATTTATGTGGAACCATGGAAGCACTTAATGTTCCCATGGCTGACACACCAGTTGTCACCTTTTGGGAAGGGGAGATTGTTGACACCAAGAATTATACTTTCTTCACTGAAAAGTGGGAAGCAACACAAGATGATGATATAAGGCACTGGACCAAATTTCCTTCTTTTTCTGCTCTGTTGAGCCAAGTGGAGGTTGATGGTGGCAAATCATTAGATCTCAGCAACTATCAGTACGTATTCATGAGATGGAAGGAACAATACTTTGTGAATGTTGGCACAGACTGTGGTTTGACTATAGCCGGCTTTTACTATGTGTGTTTCTCATGTAGTGATGGCTCCATCAACGGCTACTATTATGATCCTAATAGCAGTCCGTTCCAGAAACTTGAGCTGAAATCCACAAATGAGGGAAGATTTGGAGTCAGTTTTTCGTCATATGAACTGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vac_ImportDeg

Protein Analysis

216

Amino Acids

24.03

Weight (kDa)

4.6

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vac_ImportDeg PF09783 31 - 199 1.3e-51 Vacuolar import and degradation protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 29
AciI CCGC 2 cut(s) 14, 27
AclWI GGATC 1 cut(s) 551
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 1 cut(s) 353
AfaI GTAC 2 cut(s) 118, 431
AfiI CCNNNNNNNGG 1 cut(s) 388
AjnI CCWGG 1 cut(s) 102
AluBI AGCT 1 cut(s) 589
AluI AGCT 1 cut(s) 589
Alw26I GTCTC 1 cut(s) 14
AlwI GGATC 1 cut(s) 551
Ama87I CYCGRG 1 cut(s) 112
AoxI GGCC 1 cut(s) 100
ApoI RAATTY 1 cut(s) 353
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 348
AsuHPI GGTGA 1 cut(s) 247
AvaI CYCGRG 1 cut(s) 112
AvaII GGWCC 1 cut(s) 348
BaeI ACNNNNGTAYC 2 cut(s) 164, 197
BalI TGGCCA 1 cut(s) 102
BarI GAAGNNNNNNTAC 2 cut(s) 440, 472
BccI CCATC 4 cut(s) 389, 438, 524, 545
BceAI ACGGC 1 cut(s) 559
BciT130I CCWGG 1 cut(s) 104
BciVI GTATCC 1 cut(s) 165
BcoDI GTCTC 1 cut(s) 14
BfmI CTRYAG 1 cut(s) 492
BfuI GTATCC 1 cut(s) 165
BglII AGATCT 1 cut(s) 412
BisI GCNGC 1 cut(s) 15
BlsI GCNGC 1 cut(s) 16
Bme1390I CCNGG 1 cut(s) 104
Bme18I GGWCC 1 cut(s) 348
BmeT110I CYCGRG 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 348
BmiI GGNNCC 3 cut(s) 52, 211, 535
BmrFI CCNGG 1 cut(s) 104
BpuEI CTTGAG 2 cut(s) 209, 605
BsaAI YACGTR 1 cut(s) 433
BsaI GGTCTC 1 cut(s) 14
BsaJI CCNNGG 3 cut(s) 103, 213, 234
BsaXI ACNNNNNCTCC 2 cut(s) 58, 88
Bsc4I CCNNNNNNNGG 1 cut(s) 388
Bse118I RCCGGY 2 cut(s) 4, 497
Bse1I ACTGG 2 cut(s) 248, 350
BseBI CCWGG 1 cut(s) 104
BseDI CCNNGG 3 cut(s) 103, 213, 234
BseLI CCNNNNNNNGG 1 cut(s) 388
BseMII CTCAG 2 cut(s) 134, 430
BseNI ACTGG 2 cut(s) 248, 350
BseRI GAGGAG 2 cut(s) 24, 63
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 112
BsiSI CCGG 2 cut(s) 5, 498
BslI CCNNNNNNNGG 1 cut(s) 388
BsmAI GTCTC 1 cut(s) 14
BsnI GGCC 1 cut(s) 102
Bso31I GGTCTC 1 cut(s) 14
BsoBI CYCGRG 1 cut(s) 112
Bsp143I GATC 3 cut(s) 184, 412, 556
Bsp19I CCATGG 2 cut(s) 213, 234
BspACI CCGC 2 cut(s) 14, 27
BspANI GGCC 1 cut(s) 102
BspCNI CTCAG 2 cut(s) 133, 429
BspFNI CGCG 1 cut(s) 29
BspHI TCATGA 1 cut(s) 438
BspLI GGNNCC 3 cut(s) 52, 211, 535
BspPI GGATC 1 cut(s) 551
BspTNI GGTCTC 1 cut(s) 14
BsrFI RCCGGY 2 cut(s) 4, 497
BsrI ACTGG 2 cut(s) 248, 350
BssAI RCCGGY 2 cut(s) 4, 497
BssECI CCNNGG 3 cut(s) 103, 213, 234
BssMI GATC 3 cut(s) 184, 412, 556
BssT1I CCWWGG 2 cut(s) 213, 234
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 1 cut(s) 482
BstBAI YACGTR 1 cut(s) 433
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 2 cut(s) 120, 416
BstDSI CCRYGG 2 cut(s) 213, 234
BstFNI CGCG 1 cut(s) 29
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 3 cut(s) 187, 415, 559
BstMAI GTCTC 1 cut(s) 14
BstMBI GATC 3 cut(s) 184, 412, 556
BstNI CCWGG 1 cut(s) 104
BstSCI CCNGG 1 cut(s) 102
BstSFI CTRYAG 1 cut(s) 492
BstSNI TACGTA 1 cut(s) 433
BstUI CGCG 1 cut(s) 29
BstX2I RGATCY 1 cut(s) 412
BstYI RGATCY 1 cut(s) 412
BsuI GTATCC 1 cut(s) 165
BsuRI GGCC 1 cut(s) 102
BtgI CCRYGG 2 cut(s) 213, 234
BtsIMutI CAGTG 2 cut(s) 303, 343
Cac8I GCNNGC 1 cut(s) 499
CciI TCATGA 1 cut(s) 438
CfoI GCGC 1 cut(s) 31
Cfr10I RCCGGY 2 cut(s) 4, 497
Cfr13I GGNCC 1 cut(s) 348
Csp6I GTAC 2 cut(s) 117, 430
CviAII CATG 4 cut(s) 214, 235, 439, 522
CviQI GTAC 2 cut(s) 117, 430
DdeI CTNAG 2 cut(s) 120, 416
DpnI GATC 3 cut(s) 186, 414, 558
DpnII GATC 3 cut(s) 184, 412, 556
EaeI YGGCCR 1 cut(s) 100
Eco105I TACGTA 1 cut(s) 433
Eco130I CCWWGG 2 cut(s) 213, 234
Eco31I GGTCTC 1 cut(s) 14
Eco47I GGWCC 1 cut(s) 348
Eco88I CYCGRG 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 102
EcoT14I CCWWGG 2 cut(s) 213, 234
ErhI CCWWGG 2 cut(s) 213, 234
FaeI CATG 4 cut(s) 217, 238, 442, 525
FatI CATG 4 cut(s) 213, 234, 438, 521
FauNDI CATATG 1 cut(s) 634
Fnu4HI GCNGC 1 cut(s) 15
Fsp4HI GCNGC 1 cut(s) 15
GlaI GCGC 1 cut(s) 30
GluI GCNGC 1 cut(s) 15
HaeIII GGCC 1 cut(s) 102
HapII CCGG 2 cut(s) 5, 498
HhaI GCGC 1 cut(s) 31
Hin1II CATG 4 cut(s) 217, 238, 442, 525
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HinfI GANTC 2 cut(s) 133, 618
HpaII CCGG 2 cut(s) 5, 498
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 2 cut(s) 62, 280
Hpy188I TCNGA 2 cut(s) 123, 171
Hpy188III TCNNGA 3 cut(s) 188, 439, 577
Hpy8I GTNNAC 2 cut(s) 62, 280
HpyAV CCTTC 3 cut(s) 260, 369, 442
HpyCH4III ACNGT 1 cut(s) 482
HpyCH4IV ACGT 1 cut(s) 432
HpyCH4V TGCA 2 cut(s) 139, 643
HpyF3I CTNAG 2 cut(s) 120, 416
HpySE526I ACGT 1 cut(s) 432
Hsp92II CATG 4 cut(s) 217, 238, 442, 525
HspAI GCGC 1 cut(s) 29
KroI GCCGGC 1 cut(s) 497
KroNI GCCGGC 1 cut(s) 499
Kzo9I GATC 3 cut(s) 184, 412, 556
LmnI GCTCC 1 cut(s) 539
LpnPI CCDG 9 cut(s) 18, 33, 70, 89, 116, 261, 331, 511, 590
MaeII ACGT 1 cut(s) 432
MaeIII GTNAC 1 cut(s) 253
MalI GATC 3 cut(s) 186, 414, 558
MboI GATC 3 cut(s) 184, 412, 556
MboII GAAGA 2 cut(s) 292, 621
MflI RGATCY 1 cut(s) 412
MlsI TGGCCA 1 cut(s) 102
MluCI AATT 2 cut(s) 289, 353
MluNI TGGCCA 1 cut(s) 102
MlyI GAGTC 2 cut(s) 142, 627
MnlI CCTC 7 cut(s) 34, 42, 45, 84, 142, 382, 598
Mox20I TGGCCA 1 cut(s) 102
MroNI GCCGGC 1 cut(s) 497
MscI TGGCCA 1 cut(s) 102
MseI TTAA 2 cut(s) 92, 225
Msp20I TGGCCA 1 cut(s) 102
MspI CCGG 2 cut(s) 5, 498
MspR9I CCNGG 1 cut(s) 104
MvaI CCWGG 1 cut(s) 104
MvnI CGCG 1 cut(s) 29
NaeI GCCGGC 1 cut(s) 499
NcoI CCATGG 2 cut(s) 213, 234
NdeI CATATG 1 cut(s) 634
NdeII GATC 3 cut(s) 184, 412, 556
NgoMIV GCCGGC 1 cut(s) 497
NlaIII CATG 4 cut(s) 217, 238, 442, 525
NlaIV GGNNCC 3 cut(s) 52, 211, 535
NmuCI GTSAC 1 cut(s) 253
PagI TCATGA 1 cut(s) 438
PdiI GCCGGC 1 cut(s) 499
PkrI GCNGC 1 cut(s) 16
PleI GAGTC 2 cut(s) 141, 626
PpsI GAGTC 2 cut(s) 141, 626
Ppu21I YACGTR 1 cut(s) 433
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 3 cut(s) 52, 211, 535
PspPI GGNCC 1 cut(s) 348
PsuI RGATCY 1 cut(s) 412
RsaI GTAC 2 cut(s) 118, 431
RsaNI GTAC 2 cut(s) 117, 430
SaqAI TTAA 2 cut(s) 92, 225
SatI GCNGC 1 cut(s) 15
Sau3AI GATC 3 cut(s) 184, 412, 556
Sau96I GGNCC 1 cut(s) 348
SchI GAGTC 2 cut(s) 142, 627
ScrFI CCNGG 1 cut(s) 104
SetI ASST 7 cut(s) 26, 52, 59, 260, 393, 435, 591
SfcI CTRYAG 1 cut(s) 492
SinI GGWCC 1 cut(s) 348
SmlI CTYRAG 2 cut(s) 188, 584
SmoI CTYRAG 2 cut(s) 188, 584
SnaBI TACGTA 1 cut(s) 433
Sse9I AATT 2 cut(s) 289, 353
SsiI CCGC 2 cut(s) 14, 27
StyD4I CCNGG 1 cut(s) 102
StyI CCWWGG 2 cut(s) 213, 234
TaaI ACNGT 1 cut(s) 482
TaiI ACGT 1 cut(s) 435
TaqI TCGA 1 cut(s) 131
TasI AATT 2 cut(s) 289, 353
TauI GCSGC 1 cut(s) 17
Tru1I TTAA 2 cut(s) 92, 225
Tru9I TTAA 2 cut(s) 92, 225
TscAI CASTG 2 cut(s) 310, 350
TseFI GTSAC 1 cut(s) 253
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 1 cut(s) 427
TspGWI ACGGA 1 cut(s) 561
TspRI CASTG 2 cut(s) 310, 350
VpaK11BI GGWCC 1 cut(s) 348
XapI RAATTY 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.