Rw3G011800

Glucose-induced degradation protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005099
Physical Location & Seq
Forward (+)
11627345 .. 11629293
1949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G011800.1

Sequence Viewer

Length: 546 bp
ATGCCGGTCAGAGCGGCAGAGACCTCCGCGCCTCCTCAACACCATTCAGGTTCAAACTGCGGACAGACATCTCCCCCACCCTTCACACTTTTAAGCGTTGGCCAGGGCTTCTCGGGTACTCAAAATGTTTCGAGTCTGCAGAAAGAAGAAGCTTGGAGAGTAAATGTTCGGATACAAGGGTGTGATCTTGAGAATGGTTATCTGTGTGGAACCATGGAAGCACTTAATGTTCCCATGGCTGACACACCAGTCGTCACCTTTTGGGAAGGGGAGATTGTTGACACCAAGAATTATACTTTCTTCACTGAGAAGTGGGAAGCAACACCAGAGGATGATATAAGTCACTGGACCAAATTTCCTTCTTTTTCTGCGCTGTTGAGCGAAGTGGAAGTTGATGGTGGCAAATCATTAGATCTCAGCAACTATCCATACCTAATCACGAGATGGAAGGAGCAATATTTTGTGAATGTTGGCACAGACTGTGGTTTGACTAGAGCCGGCTTTTTCTATGTGTGTTTCTCAGCCCGTTCCAGAAGCTTGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vac_ImportDeg

Protein Analysis

181

Amino Acids

20.11

Weight (kDa)

4.81

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vac_ImportDeg PF09783 31 - 178 5.3e-35 Vacuolar import and degradation protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 248
AccBSI CCGCTC 1 cut(s) 14
AccII CGCG 1 cut(s) 29
AciI CCGC 3 cut(s) 14, 27, 60
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 1 cut(s) 353
AfaI GTAC 1 cut(s) 118
AgsI TTSAA 1 cut(s) 54
AjnI CCWGG 1 cut(s) 102
AluBI AGCT 3 cut(s) 152, 537, 543
AluI AGCT 3 cut(s) 152, 537, 543
Alw26I GTCTC 1 cut(s) 14
Ama87I CYCGRG 1 cut(s) 112
AoxI GGCC 1 cut(s) 100
ApoI RAATTY 1 cut(s) 353
AspLEI GCGC 2 cut(s) 31, 373
AspS9I GGNCC 1 cut(s) 348
AsuHPI GGTGA 1 cut(s) 247
AvaI CYCGRG 1 cut(s) 112
AvaII GGWCC 1 cut(s) 348
BaeI ACNNNNGTAYC 2 cut(s) 164, 197
BalI TGGCCA 1 cut(s) 102
BauI CACGAG 1 cut(s) 439
BccI CCATC 2 cut(s) 389, 438
BciT130I CCWGG 1 cut(s) 104
BciVI GTATCC 1 cut(s) 165
BcoDI GTCTC 1 cut(s) 14
BfaI CTAG 1 cut(s) 492
BfmI CTRYAG 1 cut(s) 137
BfuI GTATCC 1 cut(s) 165
BglII AGATCT 1 cut(s) 412
BisI GCNGC 1 cut(s) 15
BlsI GCNGC 1 cut(s) 16
Bme1390I CCNGG 1 cut(s) 104
Bme18I GGWCC 1 cut(s) 348
BmeT110I CYCGRG 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 348
BmiI GGNNCC 1 cut(s) 211
BmrFI CCNGG 1 cut(s) 104
BpuEI CTTGAG 1 cut(s) 209
BsaI GGTCTC 1 cut(s) 14
BsaJI CCNNGG 3 cut(s) 103, 213, 234
Bse118I RCCGGY 2 cut(s) 4, 497
Bse1I ACTGG 2 cut(s) 248, 350
BseBI CCWGG 1 cut(s) 104
BseDI CCNNGG 3 cut(s) 103, 213, 234
BseGI GGATG 1 cut(s) 337
BseMII CTCAG 3 cut(s) 297, 430, 534
BseNI ACTGG 2 cut(s) 248, 350
BseRI GAGGAG 1 cut(s) 24
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 112
BsiSI CCGG 2 cut(s) 5, 498
BsmAI GTCTC 1 cut(s) 14
BsnI GGCC 1 cut(s) 102
Bso31I GGTCTC 1 cut(s) 14
BsoBI CYCGRG 1 cut(s) 112
Bsp143I GATC 2 cut(s) 184, 412
Bsp19I CCATGG 2 cut(s) 213, 234
BspACI CCGC 3 cut(s) 14, 27, 60
BspANI GGCC 1 cut(s) 102
BspCNI CTCAG 3 cut(s) 298, 429, 533
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 1 cut(s) 211
BspMAI CTGCAG 1 cut(s) 141
BspTNI GGTCTC 1 cut(s) 14
BsrBI CCGCTC 1 cut(s) 14
BsrFI RCCGGY 2 cut(s) 4, 497
BsrI ACTGG 2 cut(s) 248, 350
BssAI RCCGGY 2 cut(s) 4, 497
BssECI CCNNGG 3 cut(s) 103, 213, 234
BssMI GATC 2 cut(s) 184, 412
BssSI CACGAG 1 cut(s) 439
BssT1I CCWWGG 2 cut(s) 213, 234
Bst2BI CACGAG 1 cut(s) 439
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 1 cut(s) 482
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 3 cut(s) 306, 416, 520
BstDSI CCRYGG 2 cut(s) 213, 234
BstF5I GGATG 1 cut(s) 337
BstFNI CGCG 1 cut(s) 29
BstHHI GCGC 2 cut(s) 31, 373
BstKTI GATC 2 cut(s) 187, 415
BstMAI GTCTC 1 cut(s) 14
BstMBI GATC 2 cut(s) 184, 412
BstNI CCWGG 1 cut(s) 104
BstSCI CCNGG 1 cut(s) 102
BstSFI CTRYAG 1 cut(s) 137
BstUI CGCG 1 cut(s) 29
BstX2I RGATCY 1 cut(s) 412
BstYI RGATCY 1 cut(s) 412
BsuI GTATCC 1 cut(s) 165
BsuRI GGCC 1 cut(s) 102
BtgI CCRYGG 2 cut(s) 213, 234
BtsCI GGATG 1 cut(s) 337
BtsIMutI CAGTG 2 cut(s) 303, 343
Cac8I GCNNGC 1 cut(s) 499
CfoI GCGC 2 cut(s) 31, 373
Cfr10I RCCGGY 2 cut(s) 4, 497
Cfr13I GGNCC 1 cut(s) 348
Csp6I GTAC 1 cut(s) 117
CviAII CATG 2 cut(s) 214, 235
CviJI RGCY 9 cut(s) 102, 108, 152, 239, 497, 501, 524, 537, 543
CviKI_1 RGCY 9 cut(s) 102, 108, 152, 239, 497, 501, 524, 537, 543
CviQI GTAC 1 cut(s) 117
DdeI CTNAG 3 cut(s) 306, 416, 520
DpnI GATC 2 cut(s) 186, 414
DpnII GATC 2 cut(s) 184, 412
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
EaeI YGGCCR 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 213, 234
Eco31I GGTCTC 1 cut(s) 14
Eco47I GGWCC 1 cut(s) 348
Eco88I CYCGRG 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 102
EcoT14I CCWWGG 2 cut(s) 213, 234
ErhI CCWWGG 2 cut(s) 213, 234
FaeI CATG 2 cut(s) 217, 238
FaiI YATR 6 cut(s) 215, 236, 294, 338, 430, 510
FatI CATG 2 cut(s) 213, 234
Fnu4HI GCNGC 1 cut(s) 15
FokI GGATG 1 cut(s) 344
Fsp4HI GCNGC 1 cut(s) 15
FspBI CTAG 1 cut(s) 492
GlaI GCGC 2 cut(s) 30, 372
GluI GCNGC 1 cut(s) 15
HaeIII GGCC 1 cut(s) 102
HapII CCGG 2 cut(s) 5, 498
HhaI GCGC 2 cut(s) 31, 373
Hin1II CATG 2 cut(s) 217, 238
Hin6I GCGC 2 cut(s) 29, 371
HinP1I GCGC 2 cut(s) 29, 371
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HindIII AAGCTT 2 cut(s) 150, 535
HinfI GANTC 1 cut(s) 133
HpaII CCGG 2 cut(s) 5, 498
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 2 cut(s) 11, 171
Hpy188III TCNNGA 3 cut(s) 188, 439, 531
Hpy8I GTNNAC 1 cut(s) 280
HpyAV CCTTC 4 cut(s) 91, 260, 369, 442
HpyCH4III ACNGT 1 cut(s) 482
HpyCH4V TGCA 1 cut(s) 139
HpyF3I CTNAG 3 cut(s) 306, 416, 520
Hsp92II CATG 2 cut(s) 217, 238
HspAI GCGC 2 cut(s) 29, 371
KroI GCCGGC 1 cut(s) 497
KroNI GCCGGC 1 cut(s) 499
Kzo9I GATC 2 cut(s) 184, 412
LmnI GCTCC 1 cut(s) 451
LpnPI CCDG 8 cut(s) 18, 33, 89, 116, 261, 331, 339, 511
MaeI CTAG 1 cut(s) 492
MaeIII GTNAC 2 cut(s) 253, 341
MalI GATC 2 cut(s) 186, 414
MbiI CCGCTC 1 cut(s) 14
MboI GATC 2 cut(s) 184, 412
MboII GAAGA 2 cut(s) 158, 292
MflI RGATCY 1 cut(s) 412
MlsI TGGCCA 1 cut(s) 102
MluCI AATT 2 cut(s) 289, 353
MluNI TGGCCA 1 cut(s) 102
MlyI GAGTC 1 cut(s) 142
MnlI CCTC 4 cut(s) 34, 42, 45, 322
Mox20I TGGCCA 1 cut(s) 102
MroNI GCCGGC 1 cut(s) 497
MscI TGGCCA 1 cut(s) 102
MseI TTAA 2 cut(s) 92, 225
Msp20I TGGCCA 1 cut(s) 102
MspI CCGG 2 cut(s) 5, 498
MspR9I CCNGG 1 cut(s) 104
MvaI CCWGG 1 cut(s) 104
MvnI CGCG 1 cut(s) 29
NaeI GCCGGC 1 cut(s) 499
NcoI CCATGG 2 cut(s) 213, 234
NdeII GATC 2 cut(s) 184, 412
NgoMIV GCCGGC 1 cut(s) 497
NlaIII CATG 2 cut(s) 217, 238
NlaIV GGNNCC 1 cut(s) 211
NmuCI GTSAC 2 cut(s) 253, 341
PdiI GCCGGC 1 cut(s) 499
PkrI GCNGC 1 cut(s) 16
PleI GAGTC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 1 cut(s) 348
PstI CTGCAG 1 cut(s) 141
PsuI RGATCY 1 cut(s) 412
RsaI GTAC 1 cut(s) 118
RsaNI GTAC 1 cut(s) 117
SaqAI TTAA 2 cut(s) 92, 225
SatI GCNGC 1 cut(s) 15
Sau3AI GATC 2 cut(s) 184, 412
Sau96I GGNCC 1 cut(s) 348
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 1 cut(s) 104
SetI ASST 7 cut(s) 26, 52, 154, 260, 435, 539, 545
SfcI CTRYAG 1 cut(s) 137
SinI GGWCC 1 cut(s) 348
SmlI CTYRAG 2 cut(s) 188, 538
SmoI CTYRAG 2 cut(s) 188, 538
Sse9I AATT 2 cut(s) 289, 353
SsiI CCGC 3 cut(s) 14, 27, 60
SspI AATATT 1 cut(s) 458
SspMI CTAG 1 cut(s) 492
StyD4I CCNGG 1 cut(s) 102
StyI CCWWGG 2 cut(s) 213, 234
TaaI ACNGT 1 cut(s) 482
TaqI TCGA 1 cut(s) 131
TasI AATT 2 cut(s) 289, 353
TauI GCSGC 1 cut(s) 17
Tru1I TTAA 2 cut(s) 92, 225
Tru9I TTAA 2 cut(s) 92, 225
TscAI CASTG 2 cut(s) 310, 350
TseFI GTSAC 2 cut(s) 253, 341
Tsp45I GTSAC 2 cut(s) 253, 341
TspRI CASTG 2 cut(s) 310, 350
VpaK11BI GGWCC 1 cut(s) 348
XapI RAATTY 1 cut(s) 353
XspI CTAG 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.