MD04G1133400.v1.1

Glucose-induced degradation protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005099
Physical Location & Seq
Reverse (-)
22004442 .. 22007044
2603 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1133400.v1.1.491

Sequence Viewer

Length: 645 bp
ATGCCGGTGAGAGTGGCGGAAGCCTCCGCGCCTTCTCAAACCTCAGGTTCAAATGGTGGACAGACAGCTCCTCCAGCCTGCACACTTTTAAGCGTTGGACAGGCCTTCTCCGGTACGCAGAATGTTTGTAGTATGCAGAAAGATGAAGCGTGGAGAGTGAATGTTCGGATACAGGGGTGTGACCTTGAACATGGTTATCTATGTGGAACCATGGAAGCTCTTAATGTTCCTATGGCAGACACACCGGTAGTTACCTTTTGGGAAGGAGAGATTGTTGACACCAAGAATTATACTTTCTTCACTGGAAAATGGGAGGCAACGCCAGAGGATGATATAAGGCACTGGACCAAGTTTCCTTCTTTTTCTGCTCTGCTGAGCCAAGTGGAAGTTGATGGCGGCAAATCATTAGATCTGAGTAATTATCCATACATATTCATGAGATGGAAAGAGCAATATTTTGTGAATGTTGGCACAGACTGTGGGCTAACAATAGCTGGCTTTTACTATGTCTGTTTCTCTTGTAGCGATGGCTCCATCAATGGCTTCTATTATGATCCTAAAAGCAGCCCTTTTCAGAAGCTTGAGCTGAAATCCACCAACGAGGGACGATCAGGTTTCAGCTTTTCATCATATGAACTGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vac_ImportDeg

Protein Analysis

215

Amino Acids

23.74

Weight (kDa)

4.76

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vac_ImportDeg PF09783 30 - 198 8.4e-51 Vacuolar import and degradation protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 29
AciI CCGC 3 cut(s) 17, 27, 396
AclWI GGATC 1 cut(s) 548
AfaI GTAC 1 cut(s) 115
AgeI ACCGGT 1 cut(s) 244
AgsI TTSAA 2 cut(s) 51, 188
AluBI AGCT 6 cut(s) 68, 218, 494, 580, 586, 621
AluI AGCT 6 cut(s) 68, 218, 494, 580, 586, 621
AlwI GGATC 1 cut(s) 548
AoxI GGCC 1 cut(s) 102
ApeKI GCWGC 1 cut(s) 564
AsiGI ACCGGT 1 cut(s) 244
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 345
AsuHPI GGTGA 1 cut(s) 19
AvaII GGWCC 1 cut(s) 345
AxyI CCTNAGG 1 cut(s) 43
BaeI ACNNNNGTAYC 2 cut(s) 161, 194
BbvI GCAGC 1 cut(s) 576
BccI CCATC 4 cut(s) 386, 435, 521, 542
BciVI GTATCC 1 cut(s) 162
BfuI GTATCC 1 cut(s) 162
BglII AGATCT 1 cut(s) 409
BisI GCNGC 2 cut(s) 397, 565
BlpI GCTNAGC 1 cut(s) 374
BlsI GCNGC 2 cut(s) 398, 566
Bme18I GGWCC 1 cut(s) 345
BmgT120I GGNCC 1 cut(s) 345
BmiI GGNNCC 2 cut(s) 208, 532
BpmI CTGGAG 1 cut(s) 57
Bpu1102I GCTNAGC 1 cut(s) 374
BpuEI CTTGAG 1 cut(s) 602
BsaJI CCNNGG 1 cut(s) 210
BsaWI WCCGGW 2 cut(s) 110, 244
BsaXI ACNNNNNCTCC 4 cut(s) 55, 85, 258, 288
Bse118I RCCGGY 2 cut(s) 4, 244
Bse1I ACTGG 2 cut(s) 307, 347
Bse21I CCTNAGG 1 cut(s) 43
BseDI CCNNGG 1 cut(s) 210
BseGI GGATG 1 cut(s) 334
BseMII CTCAG 3 cut(s) 57, 365, 404
BseNI ACTGG 2 cut(s) 307, 347
BseRI GAGGAG 1 cut(s) 60
BseXI GCAGC 1 cut(s) 576
BsgI GTGCAG 1 cut(s) 64
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 1 cut(s) 104
BshTI ACCGGT 1 cut(s) 244
BsiSI CCGG 3 cut(s) 5, 111, 245
BslFI GGGAC 1 cut(s) 618
BsmFI GGGAC 1 cut(s) 618
BsnI GGCC 1 cut(s) 104
Bsp143I GATC 3 cut(s) 409, 553, 608
Bsp1720I GCTNAGC 1 cut(s) 374
Bsp19I CCATGG 1 cut(s) 210
BspACI CCGC 3 cut(s) 17, 27, 396
BspANI GGCC 1 cut(s) 104
BspCNI CTCAG 3 cut(s) 56, 366, 405
BspFNI CGCG 1 cut(s) 29
BspHI TCATGA 1 cut(s) 435
BspLI GGNNCC 2 cut(s) 208, 532
BspPI GGATC 1 cut(s) 548
BsrFI RCCGGY 2 cut(s) 4, 244
BsrI ACTGG 2 cut(s) 307, 347
BssAI RCCGGY 2 cut(s) 4, 244
BssECI CCNNGG 1 cut(s) 210
BssMI GATC 3 cut(s) 409, 553, 608
BssT1I CCWWGG 1 cut(s) 210
Bst4CI ACNGT 1 cut(s) 479
BstC8I GCNNGC 2 cut(s) 79, 496
BstDEI CTNAG 3 cut(s) 43, 374, 413
BstDSI CCRYGG 1 cut(s) 210
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 1 cut(s) 29
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 3 cut(s) 412, 556, 611
BstMBI GATC 3 cut(s) 409, 553, 608
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 1 cut(s) 576
BstX2I RGATCY 1 cut(s) 409
BstYI RGATCY 1 cut(s) 409
Bsu36I CCTNAGG 1 cut(s) 43
BsuI GTATCC 1 cut(s) 162
BsuRI GGCC 1 cut(s) 104
BtgI CCRYGG 1 cut(s) 210
BtgZI GCGATG 1 cut(s) 540
BtsCI GGATG 1 cut(s) 334
BtsIMutI CAGTG 2 cut(s) 300, 340
Cac8I GCNNGC 2 cut(s) 79, 496
CciI TCATGA 1 cut(s) 435
CfoI GCGC 1 cut(s) 31
Cfr10I RCCGGY 2 cut(s) 4, 244
Cfr13I GGNCC 1 cut(s) 345
Csp6I GTAC 1 cut(s) 114
CspAI ACCGGT 1 cut(s) 244
CviAII CATG 3 cut(s) 191, 211, 436
CviQI GTAC 1 cut(s) 114
DdeI CTNAG 3 cut(s) 43, 374, 413
DpnI GATC 3 cut(s) 411, 555, 610
DpnII GATC 3 cut(s) 409, 553, 608
EciI GGCGGA 1 cut(s) 32
Eco130I CCWWGG 1 cut(s) 210
Eco147I AGGCCT 1 cut(s) 104
Eco47I GGWCC 1 cut(s) 345
Eco81I CCTNAGG 1 cut(s) 43
EcoT14I CCWWGG 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 210
FaeI CATG 3 cut(s) 194, 214, 439
FalI AAGNNNNNCTT 2 cut(s) 553, 585
FaqI GGGAC 1 cut(s) 618
FatI CATG 3 cut(s) 190, 210, 435
FauNDI CATATG 1 cut(s) 631
Fnu4HI GCNGC 2 cut(s) 397, 565
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 2 cut(s) 397, 565
GlaI GCGC 1 cut(s) 30
GluI GCNGC 2 cut(s) 397, 565
GsuI CTGGAG 1 cut(s) 57
HaeIII GGCC 1 cut(s) 104
HapII CCGG 3 cut(s) 5, 111, 245
HhaI GCGC 1 cut(s) 31
Hin1II CATG 3 cut(s) 194, 214, 439
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 578
HpaII CCGG 3 cut(s) 5, 111, 245
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 2 cut(s) 59, 277
Hpy188I TCNGA 3 cut(s) 168, 414, 576
Hpy188III TCNNGA 1 cut(s) 436
Hpy8I GTNNAC 2 cut(s) 59, 277
HpyAV CCTTC 4 cut(s) 42, 115, 257, 366
HpyCH4III ACNGT 1 cut(s) 479
HpyCH4V TGCA 2 cut(s) 81, 136
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpyF3I CTNAG 3 cut(s) 43, 374, 413
Hsp92II CATG 3 cut(s) 194, 214, 439
HspAI GCGC 1 cut(s) 29
Kzo9I GATC 3 cut(s) 409, 553, 608
LmnI GCTCC 2 cut(s) 73, 536
Lsp1109I GCAGC 1 cut(s) 576
MaeIII GTNAC 2 cut(s) 179, 250
MalI GATC 3 cut(s) 411, 555, 610
MboI GATC 3 cut(s) 409, 553, 608
MboII GAAGA 1 cut(s) 289
MflI RGATCY 1 cut(s) 409
MluCI AATT 2 cut(s) 286, 418
MmeI TCCRAC 1 cut(s) 76
MnlI CCTC 6 cut(s) 34, 52, 81, 307, 319, 595
MseI TTAA 2 cut(s) 89, 222
MslI CAYNNNNRTG 1 cut(s) 434
MspI CCGG 3 cut(s) 5, 111, 245
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 1 cut(s) 74
NcoI CCATGG 1 cut(s) 210
NdeI CATATG 1 cut(s) 631
NdeII GATC 3 cut(s) 409, 553, 608
NlaIII CATG 3 cut(s) 194, 214, 439
NlaIV GGNNCC 2 cut(s) 208, 532
NmuCI GTSAC 1 cut(s) 179
PagI TCATGA 1 cut(s) 435
PceI AGGCCT 1 cut(s) 104
PinAI ACCGGT 1 cut(s) 244
PkrI GCNGC 2 cut(s) 398, 566
PspN4I GGNNCC 2 cut(s) 208, 532
PspPI GGNCC 1 cut(s) 345
PsuI RGATCY 1 cut(s) 409
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
RseI CAYNNNNRTG 1 cut(s) 434
SaqAI TTAA 2 cut(s) 89, 222
SatI GCNGC 2 cut(s) 397, 565
Sau3AI GATC 3 cut(s) 409, 553, 608
Sau96I GGNCC 1 cut(s) 345
SinI GGWCC 1 cut(s) 345
SmiMI CAYNNNNRTG 1 cut(s) 434
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
Sse9I AATT 2 cut(s) 286, 418
SseBI AGGCCT 1 cut(s) 104
SsiI CCGC 3 cut(s) 17, 27, 396
SspI AATATT 1 cut(s) 455
StuI AGGCCT 1 cut(s) 104
StyI CCWWGG 1 cut(s) 210
TaaI ACNGT 1 cut(s) 479
TasI AATT 2 cut(s) 286, 418
TauI GCSGC 1 cut(s) 399
Tru1I TTAA 2 cut(s) 89, 222
Tru9I TTAA 2 cut(s) 89, 222
TscAI CASTG 2 cut(s) 307, 347
TseFI GTSAC 1 cut(s) 179
TseI GCWGC 1 cut(s) 564
Tsp45I GTSAC 1 cut(s) 179
TspDTI ATGAA 3 cut(s) 159, 424, 615
TspRI CASTG 2 cut(s) 307, 347
VpaK11BI GGWCC 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.