Rw3G011760

Glucose-induced degradation protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005099
Physical Location & Seq
Forward (+)
11582175 .. 11587235
5061 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G011760.1

Sequence Viewer

Length: 603 bp
ATGTCCCTAGAATTAGAGGACATGACGCCATCTCAAGGTTACCAGAGCATGTGGCCGACCATGTTGCCGCCAATGCCGCTACCACCACCGAGGGGCTTCTCCGGTACTCAAAATGTTTCGAGTCTGCAGAAAGATGAAGCTTGGAGAGTAAATGTTCGGATACAAGGGTGTGATCTTGAGAATGGTTATCTATGTGGAACCATGGAAGCAAATAATGTTCCCATGGCTGACACACCGGTCGTCACCTTTTGGGAAGGGGAGATTGTTGACACCAAGAATTATACTTTTTTCACTAAGAAGTGGGAAGCAACACGAGACGATGATATAAGGCACTGGACCAAATTTCCTTCTTTTTCTGCTCTATTGAGCCTAGTGGAAGTTGATGGTGGCAACTCATTAGATCTCAGCAACTATCCATACATATTCATGAGATGGAAGGAGCAATACTTAGTGCATGTTGGCACAGACTGTGGTTTGACTATAGCCGGCTTTTACTATGTGTGTTTCTCATGTAGTGATGGCTCCATCAACAGCTTCTATTATGATCCTAATAGCAGCCCGTTCCAGAAGCTTGAGCTGAAATCCACAAATGGGGGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vac_ImportDeg

Protein Analysis

200

Amino Acids

22.72

Weight (kDa)

4.64

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vac_ImportDeg PF09783 31 - 195 2.6e-49 Vacuolar import and degradation protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 236
AciI CCGC 2 cut(s) 68, 77
AclWI GGATC 1 cut(s) 539
AcoI YGGCCR 1 cut(s) 53
AcsI RAATTY 1 cut(s) 341
AcyI GRCGYC 1 cut(s) 26
AfaI GTAC 1 cut(s) 106
AfiI CCNNNNNNNGG 3 cut(s) 35, 92, 591
AgeI ACCGGT 1 cut(s) 235
AluBI AGCT 4 cut(s) 140, 534, 571, 577
AluI AGCT 4 cut(s) 140, 534, 571, 577
Alw26I GTCTC 1 cut(s) 309
AlwI GGATC 1 cut(s) 539
AoxI GGCC 1 cut(s) 53
ApeKI GCWGC 1 cut(s) 555
ApoI RAATTY 1 cut(s) 341
AsiGI ACCGGT 1 cut(s) 235
AspS9I GGNCC 1 cut(s) 336
AsuHPI GGTGA 1 cut(s) 235
AvaII GGWCC 1 cut(s) 336
BaeI ACNNNNGTAYC 2 cut(s) 152, 185
BarI GAAGNNNNNNTAC 2 cut(s) 428, 460
BauI CACGAG 1 cut(s) 312
BbvI GCAGC 1 cut(s) 567
BccI CCATC 5 cut(s) 37, 377, 426, 512, 533
BcgI CGANNNNNNTGC 2 cut(s) 46, 80
BciVI GTATCC 1 cut(s) 153
BcoDI GTCTC 1 cut(s) 309
BfaI CTAG 2 cut(s) 8, 371
BfmI CTRYAG 2 cut(s) 125, 480
BfuI GTATCC 1 cut(s) 153
BglII AGATCT 1 cut(s) 400
BisI GCNGC 3 cut(s) 68, 77, 556
BlsI GCNGC 3 cut(s) 69, 78, 557
Bme18I GGWCC 1 cut(s) 336
BmgT120I GGNCC 1 cut(s) 336
BmiI GGNNCC 2 cut(s) 199, 523
BpuEI CTTGAG 3 cut(s) 18, 197, 593
BsaHI GRCGYC 1 cut(s) 26
BsaJI CCNNGG 3 cut(s) 89, 201, 222
BsaWI WCCGGW 2 cut(s) 101, 235
Bsc4I CCNNNNNNNGG 3 cut(s) 35, 92, 591
Bse118I RCCGGY 2 cut(s) 235, 485
Bse1I ACTGG 1 cut(s) 338
BseDI CCNNGG 3 cut(s) 89, 201, 222
BseLI CCNNNNNNNGG 3 cut(s) 35, 92, 591
BseMII CTCAG 1 cut(s) 418
BseNI ACTGG 1 cut(s) 338
BseXI GCAGC 1 cut(s) 567
Bsh1285I CGRYCG 1 cut(s) 240
BshFI GGCC 1 cut(s) 55
BshTI ACCGGT 1 cut(s) 235
BsiEI CGRYCG 1 cut(s) 240
BsiSI CCGG 3 cut(s) 102, 236, 486
BslI CCNNNNNNNGG 3 cut(s) 35, 92, 591
BsmAI GTCTC 1 cut(s) 309
BsmBI CGTCTC 1 cut(s) 309
BsnI GGCC 1 cut(s) 55
Bsp143I GATC 3 cut(s) 172, 400, 544
Bsp19I CCATGG 2 cut(s) 201, 222
BspACI CCGC 2 cut(s) 68, 77
BspANI GGCC 1 cut(s) 55
BspCNI CTCAG 1 cut(s) 417
BspHI TCATGA 1 cut(s) 426
BspLI GGNNCC 2 cut(s) 199, 523
BspMAI CTGCAG 1 cut(s) 129
BspPI GGATC 1 cut(s) 539
BsrFI RCCGGY 2 cut(s) 235, 485
BsrI ACTGG 1 cut(s) 338
BssAI RCCGGY 2 cut(s) 235, 485
BssECI CCNNGG 3 cut(s) 89, 201, 222
BssMI GATC 3 cut(s) 172, 400, 544
BssNI GRCGYC 1 cut(s) 26
BssSI CACGAG 1 cut(s) 312
BssT1I CCWWGG 2 cut(s) 201, 222
Bst2BI CACGAG 1 cut(s) 312
Bst4CI ACNGT 1 cut(s) 470
BstACI GRCGYC 1 cut(s) 26
BstC8I GCNNGC 1 cut(s) 487
BstDEI CTNAG 3 cut(s) 294, 404, 448
BstDSI CCRYGG 2 cut(s) 201, 222
BstEII GGTNACC 1 cut(s) 38
BstKTI GATC 3 cut(s) 175, 403, 547
BstMAI GTCTC 1 cut(s) 309
BstMBI GATC 3 cut(s) 172, 400, 544
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 2 cut(s) 73, 76
BstNSI RCATGY 2 cut(s) 52, 458
BstPI GGTNACC 1 cut(s) 38
BstSFI CTRYAG 2 cut(s) 125, 480
BstV1I GCAGC 1 cut(s) 567
BstX2I RGATCY 1 cut(s) 400
BstYI RGATCY 1 cut(s) 400
BsuI GTATCC 1 cut(s) 153
BsuRI GGCC 1 cut(s) 55
BtgI CCRYGG 2 cut(s) 201, 222
BtsIMutI CAGTG 1 cut(s) 331
Cac8I GCNNGC 1 cut(s) 487
CciI TCATGA 1 cut(s) 426
Cfr10I RCCGGY 2 cut(s) 235, 485
Cfr13I GGNCC 1 cut(s) 336
CseI GACGC 1 cut(s) 34
Csp6I GTAC 1 cut(s) 105
CspAI ACCGGT 1 cut(s) 235
CviAII CATG 8 cut(s) 22, 49, 61, 202, 223, 427, 455, 510
CviQI GTAC 1 cut(s) 105
DdeI CTNAG 3 cut(s) 294, 404, 448
DpnI GATC 3 cut(s) 174, 402, 546
DpnII GATC 3 cut(s) 172, 400, 544
DrdI GACNNNNNNGTC 1 cut(s) 236
DseDI GACNNNNNNGTC 1 cut(s) 236
EaeI YGGCCR 1 cut(s) 53
Eco130I CCWWGG 2 cut(s) 201, 222
Eco47I GGWCC 1 cut(s) 336
Eco91I GGTNACC 1 cut(s) 38
EcoO65I GGTNACC 1 cut(s) 38
EcoT14I CCWWGG 2 cut(s) 201, 222
ErhI CCWWGG 2 cut(s) 201, 222
Esp3I CGTCTC 1 cut(s) 309
FaeI CATG 8 cut(s) 25, 52, 64, 205, 226, 430, 458, 513
FatI CATG 8 cut(s) 21, 48, 60, 201, 222, 426, 454, 509
Fnu4HI GCNGC 3 cut(s) 68, 77, 556
Fsp4HI GCNGC 3 cut(s) 68, 77, 556
FspBI CTAG 2 cut(s) 8, 371
GluI GCNGC 3 cut(s) 68, 77, 556
HaeIII GGCC 1 cut(s) 55
HapII CCGG 3 cut(s) 102, 236, 486
HgaI GACGC 1 cut(s) 34
Hin1I GRCGYC 1 cut(s) 26
Hin1II CATG 8 cut(s) 25, 52, 64, 205, 226, 430, 458, 513
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HindIII AAGCTT 2 cut(s) 138, 569
HinfI GANTC 1 cut(s) 121
HpaII CCGG 3 cut(s) 102, 236, 486
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 1 cut(s) 159
Hpy188III TCNNGA 3 cut(s) 176, 427, 565
Hpy8I GTNNAC 1 cut(s) 268
HpyAV CCTTC 3 cut(s) 248, 357, 430
HpyCH4III ACNGT 1 cut(s) 470
HpyCH4V TGCA 2 cut(s) 127, 454
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 76
HpyF3I CTNAG 3 cut(s) 294, 404, 448
Hsp92I GRCGYC 1 cut(s) 26
Hsp92II CATG 8 cut(s) 25, 52, 64, 205, 226, 430, 458, 513
KroI GCCGGC 1 cut(s) 485
KroNI GCCGGC 1 cut(s) 487
Kzo9I GATC 3 cut(s) 172, 400, 544
LmnI GCTCC 2 cut(s) 439, 527
LpnPI CCDG 6 cut(s) 56, 115, 249, 319, 499, 578
Lsp1109I GCAGC 1 cut(s) 567
MaeI CTAG 2 cut(s) 8, 371
MaeIII GTNAC 2 cut(s) 38, 241
MalI GATC 3 cut(s) 174, 402, 546
MboI GATC 3 cut(s) 172, 400, 544
MflI RGATCY 1 cut(s) 400
MluCI AATT 3 cut(s) 11, 277, 341
MlyI GAGTC 1 cut(s) 130
MnlI CCTC 2 cut(s) 10, 84
MroNI GCCGGC 1 cut(s) 485
MslI CAYNNNNRTG 1 cut(s) 425
MspI CCGG 3 cut(s) 102, 236, 486
MwoI GCNNNNNNNGC 2 cut(s) 73, 76
NaeI GCCGGC 1 cut(s) 487
NcoI CCATGG 2 cut(s) 201, 222
NdeII GATC 3 cut(s) 172, 400, 544
NgoMIV GCCGGC 1 cut(s) 485
NlaIII CATG 8 cut(s) 25, 52, 64, 205, 226, 430, 458, 513
NlaIV GGNNCC 2 cut(s) 199, 523
NmuCI GTSAC 1 cut(s) 241
NspI RCATGY 2 cut(s) 52, 458
PagI TCATGA 1 cut(s) 426
PdiI GCCGGC 1 cut(s) 487
PinAI ACCGGT 1 cut(s) 235
PkrI GCNGC 3 cut(s) 69, 78, 557
PleI GAGTC 1 cut(s) 129
PpsI GAGTC 1 cut(s) 129
PspEI GGTNACC 1 cut(s) 38
PspN4I GGNNCC 2 cut(s) 199, 523
PspPI GGNCC 1 cut(s) 336
PstI CTGCAG 1 cut(s) 129
PsuI RGATCY 1 cut(s) 400
RsaI GTAC 1 cut(s) 106
RsaNI GTAC 1 cut(s) 105
RseI CAYNNNNRTG 1 cut(s) 425
SatI GCNGC 3 cut(s) 68, 77, 556
Sau3AI GATC 3 cut(s) 172, 400, 544
Sau96I GGNCC 1 cut(s) 336
SchI GAGTC 1 cut(s) 130
SetI ASST 6 cut(s) 40, 142, 248, 536, 573, 579
SfcI CTRYAG 2 cut(s) 125, 480
SinI GGWCC 1 cut(s) 336
SmiMI CAYNNNNRTG 1 cut(s) 425
SmlI CTYRAG 3 cut(s) 33, 176, 572
SmoI CTYRAG 3 cut(s) 33, 176, 572
Sse9I AATT 3 cut(s) 11, 277, 341
SsiI CCGC 2 cut(s) 68, 77
SspMI CTAG 2 cut(s) 8, 371
StyI CCWWGG 2 cut(s) 201, 222
TaaI ACNGT 1 cut(s) 470
TaqI TCGA 1 cut(s) 119
TasI AATT 3 cut(s) 11, 277, 341
TauI GCSGC 2 cut(s) 70, 79
TscAI CASTG 1 cut(s) 338
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 1 cut(s) 555
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 2 cut(s) 150, 415
TspRI CASTG 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 336
XapI RAATTY 1 cut(s) 341
XceI RCATGY 2 cut(s) 52, 458
XspI CTAG 2 cut(s) 8, 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.