MD12G1146200.v1.1

Glucose-induced degradation protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005099
Physical Location & Seq
Reverse (-)
22503932 .. 22506427
2496 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1146200.v1.1.491

Sequence Viewer

Length: 747 bp
ATGACCGCGGGTATTTTGCCCATCCCTAACTCTGGATGGCGTATTGGATTGGAGGTAGCATCTGAGTCGCCTTACTCCGTTCCGATTGAATTAATCAAAGAAATGCCGGTGAGAGTGGCGGAGACGTCCGCGCCTTCTCAACTTTCAGGTTCAAATTGTGGACAGACAGCTCCTCCAGCTTGCACACTTTTAAGCGTTGGACAGGCCTTCTCCGGTACGCAGAACGTTTCTAGTCCGCAGAAAGATGAAGCGTGGAGAGTGAATGTTCGGATACAGGGGTGTGACCTTGAGTATGGTTATCTATGTGGAACCATGGAAGCTCTTAACGTTCCTATGGCAGACACACCGGTAGTTACCTTTTGGGAAGGAGAGATCGTTGACACCAAGAATTATACTTTCTTCACTGGAAAATGGGAAGCAACGCCAGAGGATGATATAAGGCACTGGACCAAGTTTCCTTCTTTTTCCTCTCTTCTGAGCCAAGTGGAAGTTGATGGTGGCAAATCATTAGATCTGAGTAATTATCCATACATATTCATGAGATGGAAAGAGCAATACTTTGTGAATGTTGGCACAGACTGTGGGCTGACTATAGCTGGCTTTTACTATGTGTGTTTTTCTTGTAGCGATGGCTCCATCAATGGCTTCTATTATGATCCTAACAGCAGCCCTTTTCAGAAGCTTGAGCTGAAATCCACTAACGAGGAACGATCAGGTTTCAGCTTTTCATCATATGAACTGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vac_ImportDeg

Protein Analysis

249

Amino Acids

27.53

Weight (kDa)

4.53

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vac_ImportDeg PF09783 64 - 232 4.7e-50 Vacuolar import and degradation protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 128
AccII CGCG 2 cut(s) 8, 131
AciI CCGC 5 cut(s) 6, 8, 119, 129, 236
AclI AACGTT 2 cut(s) 225, 327
AclWI GGATC 1 cut(s) 650
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 1 cut(s) 217
AfiI CCNNNNNNNGG 1 cut(s) 32
AgeI ACCGGT 1 cut(s) 346
AgsI TTSAA 2 cut(s) 89, 153
AluBI AGCT 7 cut(s) 170, 179, 320, 596, 682, 688, 723
AluI AGCT 7 cut(s) 170, 179, 320, 596, 682, 688, 723
Alw26I GTCTC 1 cut(s) 116
AlwI GGATC 1 cut(s) 650
AoxI GGCC 1 cut(s) 204
ApeKI GCWGC 1 cut(s) 666
AseI ATTAAT 1 cut(s) 92
AsiGI ACCGGT 1 cut(s) 346
AspLEI GCGC 1 cut(s) 133
AspS9I GGNCC 1 cut(s) 447
AsuHPI GGTGA 1 cut(s) 121
AvaII GGWCC 1 cut(s) 447
BaeI ACNNNNGTAYC 2 cut(s) 263, 296
BbvI GCAGC 1 cut(s) 678
BccI CCATC 6 cut(s) 29, 30, 488, 537, 623, 644
BcgI CGANNNNNNTGC 2 cut(s) 48, 82
BciVI GTATCC 1 cut(s) 264
BcoDI GTCTC 1 cut(s) 116
BfaI CTAG 1 cut(s) 231
BfmI CTRYAG 1 cut(s) 591
BfuI GTATCC 1 cut(s) 264
BglII AGATCT 1 cut(s) 511
BisI GCNGC 1 cut(s) 667
BlsI GCNGC 1 cut(s) 668
Bme18I GGWCC 1 cut(s) 447
BmgT120I GGNCC 1 cut(s) 447
BmiI GGNNCC 2 cut(s) 310, 634
BmsI GCATC 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 159
BpuEI CTTGAG 2 cut(s) 308, 704
BsaHI GRCGYC 1 cut(s) 125
BsaJI CCNNGG 2 cut(s) 6, 312
BsaWI WCCGGW 2 cut(s) 212, 346
BsaXI ACNNNNNCTCC 4 cut(s) 157, 187, 360, 390
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse118I RCCGGY 2 cut(s) 106, 346
Bse1I ACTGG 2 cut(s) 409, 449
BseDI CCNNGG 2 cut(s) 6, 312
BseGI GGATG 3 cut(s) 21, 41, 436
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMII CTCAG 3 cut(s) 54, 467, 506
BseNI ACTGG 2 cut(s) 409, 449
BseRI GAGGAG 1 cut(s) 162
BseXI GCAGC 1 cut(s) 678
Bsh1236I CGCG 2 cut(s) 8, 131
BshFI GGCC 1 cut(s) 206
BshTI ACCGGT 1 cut(s) 346
BsiSI CCGG 3 cut(s) 107, 213, 347
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 1 cut(s) 116
BsmBI CGTCTC 1 cut(s) 116
BsnI GGCC 1 cut(s) 206
Bsp143I GATC 4 cut(s) 372, 511, 655, 710
Bsp19I CCATGG 1 cut(s) 312
BspACI CCGC 5 cut(s) 6, 8, 119, 129, 236
BspANI GGCC 1 cut(s) 206
BspCNI CTCAG 3 cut(s) 55, 468, 507
BspFNI CGCG 2 cut(s) 8, 131
BspHI TCATGA 1 cut(s) 537
BspLI GGNNCC 2 cut(s) 310, 634
BspPI GGATC 1 cut(s) 650
BsrFI RCCGGY 2 cut(s) 106, 346
BsrI ACTGG 2 cut(s) 409, 449
BssAI RCCGGY 2 cut(s) 106, 346
BssECI CCNNGG 2 cut(s) 6, 312
BssMI GATC 4 cut(s) 372, 511, 655, 710
BssNI GRCGYC 1 cut(s) 125
BssT1I CCWWGG 1 cut(s) 312
Bst4CI ACNGT 1 cut(s) 581
Bst6I CTCTTC 1 cut(s) 477
BstACI GRCGYC 1 cut(s) 125
BstC8I GCNNGC 2 cut(s) 181, 598
BstDEI CTNAG 3 cut(s) 63, 476, 515
BstDSI CCRYGG 2 cut(s) 6, 312
BstF5I GGATG 3 cut(s) 21, 41, 436
BstFNI CGCG 2 cut(s) 8, 131
BstHHI GCGC 1 cut(s) 133
BstKTI GATC 4 cut(s) 375, 514, 658, 713
BstMAI GTCTC 1 cut(s) 116
BstMBI GATC 4 cut(s) 372, 511, 655, 710
BstMWI GCNNNNNNNGC 1 cut(s) 176
BstSFI CTRYAG 1 cut(s) 591
BstUI CGCG 2 cut(s) 8, 131
BstV1I GCAGC 1 cut(s) 678
BstX2I RGATCY 1 cut(s) 511
BstYI RGATCY 1 cut(s) 511
BsuI GTATCC 1 cut(s) 264
BsuRI GGCC 1 cut(s) 206
BtgI CCRYGG 2 cut(s) 6, 312
BtgZI GCGATG 1 cut(s) 642
BtsCI GGATG 3 cut(s) 21, 41, 436
BtsIMutI CAGTG 2 cut(s) 402, 442
Cac8I GCNNGC 2 cut(s) 181, 598
CciI TCATGA 1 cut(s) 537
CfoI GCGC 1 cut(s) 133
Cfr10I RCCGGY 2 cut(s) 106, 346
Cfr13I GGNCC 1 cut(s) 447
Cfr42I CCGCGG 1 cut(s) 9
Csp6I GTAC 1 cut(s) 216
CspAI ACCGGT 1 cut(s) 346
CviAII CATG 2 cut(s) 313, 538
CviQI GTAC 1 cut(s) 216
DdeI CTNAG 3 cut(s) 63, 476, 515
DpnI GATC 4 cut(s) 374, 513, 657, 712
DpnII GATC 4 cut(s) 372, 511, 655, 710
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
EciI GGCGGA 1 cut(s) 134
Eco130I CCWWGG 1 cut(s) 312
Eco147I AGGCCT 1 cut(s) 206
Eco47I GGWCC 1 cut(s) 447
EcoT14I CCWWGG 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 312
Esp3I CGTCTC 1 cut(s) 116
FaeI CATG 2 cut(s) 316, 541
FatI CATG 2 cut(s) 312, 537
FauNDI CATATG 1 cut(s) 733
Fnu4HI GCNGC 1 cut(s) 667
FokI GGATG 3 cut(s) 8, 48, 443
Fsp4HI GCNGC 1 cut(s) 667
FspBI CTAG 1 cut(s) 231
GlaI GCGC 1 cut(s) 132
GluI GCNGC 1 cut(s) 667
GsuI CTGGAG 1 cut(s) 159
HaeIII GGCC 1 cut(s) 206
HapII CCGG 3 cut(s) 107, 213, 347
HhaI GCGC 1 cut(s) 133
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 2 cut(s) 316, 541
Hin6I GCGC 1 cut(s) 131
HinP1I GCGC 1 cut(s) 131
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HindIII AAGCTT 1 cut(s) 680
HinfI GANTC 1 cut(s) 65
HpaII CCGG 3 cut(s) 107, 213, 347
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 2 cut(s) 161, 379
Hpy188I TCNGA 6 cut(s) 64, 84, 270, 477, 516, 678
Hpy188III TCNNGA 2 cut(s) 33, 538
Hpy8I GTNNAC 2 cut(s) 161, 379
HpyAV CCTTC 4 cut(s) 144, 217, 359, 468
HpyCH4III ACNGT 1 cut(s) 581
HpyCH4IV ACGT 3 cut(s) 125, 225, 327
HpyCH4V TGCA 1 cut(s) 183
HpyF10VI GCNNNNNNNGC 1 cut(s) 176
HpyF3I CTNAG 3 cut(s) 63, 476, 515
HpySE526I ACGT 3 cut(s) 125, 225, 327
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 2 cut(s) 316, 541
HspAI GCGC 1 cut(s) 131
KspI CCGCGG 1 cut(s) 9
Kzo9I GATC 4 cut(s) 372, 511, 655, 710
LmnI GCTCC 2 cut(s) 175, 638
Lsp1109I GCAGC 1 cut(s) 678
LweI GCATC 1 cut(s) 68
MaeI CTAG 1 cut(s) 231
MaeII ACGT 3 cut(s) 125, 225, 327
MaeIII GTNAC 2 cut(s) 281, 352
MalI GATC 4 cut(s) 374, 513, 657, 712
MboI GATC 4 cut(s) 372, 511, 655, 710
MboII GAAGA 2 cut(s) 391, 464
MflI RGATCY 1 cut(s) 511
MluCI AATT 4 cut(s) 89, 154, 388, 520
MlyI GAGTC 1 cut(s) 74
MmeI TCCRAC 1 cut(s) 178
MnlI CCTC 5 cut(s) 46, 183, 421, 478, 697
MseI TTAA 3 cut(s) 92, 191, 324
MslI CAYNNNNRTG 1 cut(s) 536
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 3 cut(s) 107, 213, 347
MvnI CGCG 2 cut(s) 8, 131
MwoI GCNNNNNNNGC 1 cut(s) 176
NcoI CCATGG 1 cut(s) 312
NdeI CATATG 1 cut(s) 733
NdeII GATC 4 cut(s) 372, 511, 655, 710
NlaIII CATG 2 cut(s) 316, 541
NlaIV GGNNCC 2 cut(s) 310, 634
NmuCI GTSAC 1 cut(s) 281
PagI TCATGA 1 cut(s) 537
PceI AGGCCT 1 cut(s) 206
PinAI ACCGGT 1 cut(s) 346
PkrI GCNGC 1 cut(s) 668
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
PshBI ATTAAT 1 cut(s) 92
Psp1406I AACGTT 2 cut(s) 225, 327
PspN4I GGNNCC 2 cut(s) 310, 634
PspPI GGNCC 1 cut(s) 447
PsuI RGATCY 1 cut(s) 511
RsaI GTAC 1 cut(s) 217
RsaNI GTAC 1 cut(s) 216
RseI CAYNNNNRTG 1 cut(s) 536
SacII CCGCGG 1 cut(s) 9
SaqAI TTAA 3 cut(s) 92, 191, 324
SatI GCNGC 1 cut(s) 667
Sau3AI GATC 4 cut(s) 372, 511, 655, 710
Sau96I GGNCC 1 cut(s) 447
SchI GAGTC 1 cut(s) 74
SfaNI GCATC 1 cut(s) 68
SfcI CTRYAG 1 cut(s) 591
Sfr303I CCGCGG 1 cut(s) 9
SgrBI CCGCGG 1 cut(s) 9
SinI GGWCC 1 cut(s) 447
SmiMI CAYNNNNRTG 1 cut(s) 536
SmlI CTYRAG 2 cut(s) 287, 683
SmoI CTYRAG 2 cut(s) 287, 683
Sse9I AATT 4 cut(s) 89, 154, 388, 520
SseBI AGGCCT 1 cut(s) 206
SsiI CCGC 5 cut(s) 6, 8, 119, 129, 236
SspMI CTAG 1 cut(s) 231
StuI AGGCCT 1 cut(s) 206
StyI CCWWGG 1 cut(s) 312
TaaI ACNGT 1 cut(s) 581
TaiI ACGT 3 cut(s) 128, 228, 330
TasI AATT 4 cut(s) 89, 154, 388, 520
Tru1I TTAA 3 cut(s) 92, 191, 324
Tru9I TTAA 3 cut(s) 92, 191, 324
TscAI CASTG 2 cut(s) 409, 449
TseFI GTSAC 1 cut(s) 281
TseI GCWGC 1 cut(s) 666
Tsp45I GTSAC 1 cut(s) 281
TspDTI ATGAA 3 cut(s) 261, 526, 717
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 2 cut(s) 409, 449
VpaK11BI GGWCC 1 cut(s) 447
VspI ATTAAT 1 cut(s) 92
XspI CTAG 1 cut(s) 231
ZraI GACGTC 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.