Rh3DG147100

Glucose-induced degradation protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0005099
Physical Location & Seq
Forward (+)
12594230 .. 12646692
52463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG147100.1

Sequence Viewer

Length: 675 bp
ATGGCTATGGTGATCGATAGGCAAGATATTCCGGTCAGAGCGGCAGAGACCTCCGCGCCTCCTCAACACCATTCAGGTTCAAACTGCGGACAGACATCTCCCCCACCCTTCACACTTTTAAGCGTTGGCCAGGGCTTCTCGGGTACTCAAAATGTTTCGAGTCTGCAGAAAGAAGAAGCTTGGAGAGTAAATGTTCGGATACAAGGGTGTGATCTTGAGAATGGTTATCTGTGTGGAACCATGGAAGCACTTAATGTTCCCATGGCTGACACACCAGTCGTCACCTTTTGGGAAGGGGAGATTGTTGACACCAAGAATTATACTTTCTTCACTGAGAAGTGGGAAGCAACACACGATGATGATATAAGGCACTGGACCAAATTTCCTTCTTTTTCTGCTCTTTTGAGCCGAGTGGAAGTTGATGGTGGCAAATCATTAGATCTCAGCAACTATAAATACATATTCATGAGATGGAAGGAGCAATACTTTGTGAATGTTGGCACAGACTGTGGTTTGACTATAGCCGGCTTTTACTATGTGTGTTTCTCATGTAGTGATGGCTCCATCAACGGCTTTTATTATGATCCTAATAGCAGCCCGTTCCAGAAGCTTGAGCTGAAATCCACAAATGAGGGAAGATTAGGATTCAGTTTTTCGTCCTACGAACTGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vac_ImportDeg

Protein Analysis

224

Amino Acids

25.21

Weight (kDa)

4.9

Isoelectric Point (pI)

36.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vac_ImportDeg PF09783 40 - 208 2.7e-51 Vacuolar import and degradation protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 275
AccBSI CCGCTC 1 cut(s) 41
AccII CGCG 1 cut(s) 56
AciI CCGC 3 cut(s) 41, 54, 87
AclWI GGATC 1 cut(s) 578
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 1 cut(s) 380
AfaI GTAC 1 cut(s) 145
AgsI TTSAA 1 cut(s) 81
AjnI CCWGG 1 cut(s) 129
AluBI AGCT 3 cut(s) 179, 610, 616
AluI AGCT 3 cut(s) 179, 610, 616
Alw26I GTCTC 1 cut(s) 41
AlwI GGATC 1 cut(s) 578
Ama87I CYCGRG 1 cut(s) 139
AoxI GGCC 1 cut(s) 127
ApeKI GCWGC 1 cut(s) 594
ApoI RAATTY 1 cut(s) 380
AspLEI GCGC 1 cut(s) 58
AspS9I GGNCC 1 cut(s) 375
AsuHPI GGTGA 2 cut(s) 22, 274
AvaI CYCGRG 1 cut(s) 139
AvaII GGWCC 1 cut(s) 375
BaeI ACNNNNGTAYC 2 cut(s) 191, 224
BalI TGGCCA 1 cut(s) 129
BarI GAAGNNNNNNTAC 2 cut(s) 467, 499
BbvI GCAGC 1 cut(s) 606
BccI CCATC 4 cut(s) 416, 465, 551, 572
BceAI ACGGC 1 cut(s) 586
BciT130I CCWGG 1 cut(s) 131
BciVI GTATCC 1 cut(s) 192
BcoDI GTCTC 1 cut(s) 41
BfmI CTRYAG 2 cut(s) 164, 519
BfuI GTATCC 1 cut(s) 192
BglII AGATCT 1 cut(s) 439
BisI GCNGC 2 cut(s) 42, 595
BlsI GCNGC 2 cut(s) 43, 596
Bme1390I CCNGG 1 cut(s) 131
Bme18I GGWCC 1 cut(s) 375
BmeT110I CYCGRG 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 375
BmiI GGNNCC 2 cut(s) 238, 562
BmrFI CCNGG 1 cut(s) 131
BpuEI CTTGAG 2 cut(s) 236, 632
Bsa29I ATCGAT 1 cut(s) 15
BsaI GGTCTC 1 cut(s) 41
BsaJI CCNNGG 3 cut(s) 130, 240, 261
BsaWI WCCGGW 1 cut(s) 31
Bse118I RCCGGY 1 cut(s) 524
Bse1I ACTGG 2 cut(s) 275, 377
BseBI CCWGG 1 cut(s) 131
BseCI ATCGAT 1 cut(s) 15
BseDI CCNNGG 3 cut(s) 130, 240, 261
BseMII CTCAG 2 cut(s) 324, 457
BseNI ACTGG 2 cut(s) 275, 377
BseRI GAGGAG 1 cut(s) 51
BseXI GCAGC 1 cut(s) 606
Bsh1236I CGCG 1 cut(s) 56
BshFI GGCC 1 cut(s) 129
BshVI ATCGAT 1 cut(s) 15
BsiHKCI CYCGRG 1 cut(s) 139
BsiSI CCGG 2 cut(s) 32, 525
BsmAI GTCTC 1 cut(s) 41
BsnI GGCC 1 cut(s) 129
Bso31I GGTCTC 1 cut(s) 41
BsoBI CYCGRG 1 cut(s) 139
Bsp143I GATC 4 cut(s) 12, 211, 439, 583
Bsp19I CCATGG 2 cut(s) 240, 261
BspACI CCGC 3 cut(s) 41, 54, 87
BspANI GGCC 1 cut(s) 129
BspCNI CTCAG 2 cut(s) 325, 456
BspDI ATCGAT 1 cut(s) 15
BspFNI CGCG 1 cut(s) 56
BspHI TCATGA 1 cut(s) 465
BspLI GGNNCC 2 cut(s) 238, 562
BspMAI CTGCAG 1 cut(s) 168
BspPI GGATC 1 cut(s) 578
BspTNI GGTCTC 1 cut(s) 41
BsrBI CCGCTC 1 cut(s) 41
BsrFI RCCGGY 1 cut(s) 524
BsrI ACTGG 2 cut(s) 275, 377
BssAI RCCGGY 1 cut(s) 524
BssECI CCNNGG 3 cut(s) 130, 240, 261
BssMI GATC 4 cut(s) 12, 211, 439, 583
BssT1I CCWWGG 2 cut(s) 240, 261
Bst2UI CCWGG 1 cut(s) 131
Bst4CI ACNGT 1 cut(s) 509
BstC8I GCNNGC 1 cut(s) 526
BstDEI CTNAG 2 cut(s) 333, 443
BstDSI CCRYGG 2 cut(s) 240, 261
BstFNI CGCG 1 cut(s) 56
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 4 cut(s) 15, 214, 442, 586
BstMAI GTCTC 1 cut(s) 41
BstMBI GATC 4 cut(s) 12, 211, 439, 583
BstNI CCWGG 1 cut(s) 131
BstSCI CCNGG 1 cut(s) 129
BstSFI CTRYAG 2 cut(s) 164, 519
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 1 cut(s) 606
BstX2I RGATCY 1 cut(s) 439
BstYI RGATCY 1 cut(s) 439
Bsu15I ATCGAT 1 cut(s) 15
BsuI GTATCC 1 cut(s) 192
BsuRI GGCC 1 cut(s) 129
BsuTUI ATCGAT 1 cut(s) 15
BtgI CCRYGG 2 cut(s) 240, 261
BtsIMutI CAGTG 2 cut(s) 330, 370
Cac8I GCNNGC 1 cut(s) 526
CciI TCATGA 1 cut(s) 465
CfoI GCGC 1 cut(s) 58
Cfr10I RCCGGY 1 cut(s) 524
Cfr13I GGNCC 1 cut(s) 375
ClaI ATCGAT 1 cut(s) 15
Csp6I GTAC 1 cut(s) 144
CviAII CATG 4 cut(s) 241, 262, 466, 549
CviQI GTAC 1 cut(s) 144
DdeI CTNAG 2 cut(s) 333, 443
DpnI GATC 4 cut(s) 14, 213, 441, 585
DpnII GATC 4 cut(s) 12, 211, 439, 583
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 1 cut(s) 127
Eco130I CCWWGG 2 cut(s) 240, 261
Eco31I GGTCTC 1 cut(s) 41
Eco47I GGWCC 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 139
EcoRII CCWGG 1 cut(s) 129
EcoT14I CCWWGG 2 cut(s) 240, 261
ErhI CCWWGG 2 cut(s) 240, 261
FaeI CATG 4 cut(s) 244, 265, 469, 552
FatI CATG 4 cut(s) 240, 261, 465, 548
Fnu4HI GCNGC 2 cut(s) 42, 595
Fsp4HI GCNGC 2 cut(s) 42, 595
GlaI GCGC 1 cut(s) 57
GluI GCNGC 2 cut(s) 42, 595
HaeIII GGCC 1 cut(s) 129
HapII CCGG 2 cut(s) 32, 525
HhaI GCGC 1 cut(s) 58
Hin1II CATG 4 cut(s) 244, 265, 469, 552
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HindIII AAGCTT 2 cut(s) 177, 608
HinfI GANTC 2 cut(s) 160, 645
HpaII CCGG 2 cut(s) 32, 525
HphI GGTGA 2 cut(s) 22, 274
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 2 cut(s) 38, 198
Hpy188III TCNNGA 3 cut(s) 215, 466, 604
Hpy8I GTNNAC 1 cut(s) 307
HpyAV CCTTC 4 cut(s) 118, 287, 396, 469
HpyCH4III ACNGT 1 cut(s) 509
HpyCH4V TGCA 1 cut(s) 166
HpyF3I CTNAG 2 cut(s) 333, 443
Hsp92II CATG 4 cut(s) 244, 265, 469, 552
HspAI GCGC 1 cut(s) 56
KroI GCCGGC 1 cut(s) 524
KroNI GCCGGC 1 cut(s) 526
Kzo9I GATC 4 cut(s) 12, 211, 439, 583
LmnI GCTCC 2 cut(s) 478, 566
LpnPI CCDG 8 cut(s) 45, 60, 116, 143, 288, 358, 538, 617
Lsp1109I GCAGC 1 cut(s) 606
MaeIII GTNAC 1 cut(s) 280
MalI GATC 4 cut(s) 14, 213, 441, 585
MbiI CCGCTC 1 cut(s) 41
MboI GATC 4 cut(s) 12, 211, 439, 583
MboII GAAGA 3 cut(s) 185, 319, 648
MflI RGATCY 1 cut(s) 439
MlsI TGGCCA 1 cut(s) 129
MluCI AATT 2 cut(s) 316, 380
MluNI TGGCCA 1 cut(s) 129
MlyI GAGTC 1 cut(s) 169
MnlI CCTC 4 cut(s) 61, 69, 72, 625
Mox20I TGGCCA 1 cut(s) 129
MroNI GCCGGC 1 cut(s) 524
MscI TGGCCA 1 cut(s) 129
MseI TTAA 2 cut(s) 119, 252
MslI CAYNNNNRTG 2 cut(s) 357, 464
Msp20I TGGCCA 1 cut(s) 129
MspI CCGG 2 cut(s) 32, 525
MspR9I CCNGG 1 cut(s) 131
MvaI CCWGG 1 cut(s) 131
MvnI CGCG 1 cut(s) 56
NaeI GCCGGC 1 cut(s) 526
NcoI CCATGG 2 cut(s) 240, 261
NdeII GATC 4 cut(s) 12, 211, 439, 583
NgoMIV GCCGGC 1 cut(s) 524
NlaIII CATG 4 cut(s) 244, 265, 469, 552
NlaIV GGNNCC 2 cut(s) 238, 562
NmeAIII GCCGAG 1 cut(s) 434
NmuCI GTSAC 1 cut(s) 280
PagI TCATGA 1 cut(s) 465
PdiI GCCGGC 1 cut(s) 526
PfeI GAWTC 1 cut(s) 645
PkrI GCNGC 2 cut(s) 43, 596
PleI GAGTC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 168
Psp6I CCWGG 1 cut(s) 129
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 2 cut(s) 238, 562
PspPI GGNCC 1 cut(s) 375
PstI CTGCAG 1 cut(s) 168
PsuI RGATCY 1 cut(s) 439
RsaI GTAC 1 cut(s) 145
RsaNI GTAC 1 cut(s) 144
RseI CAYNNNNRTG 2 cut(s) 357, 464
SaqAI TTAA 2 cut(s) 119, 252
SatI GCNGC 2 cut(s) 42, 595
Sau3AI GATC 4 cut(s) 12, 211, 439, 583
Sau96I GGNCC 1 cut(s) 375
SchI GAGTC 1 cut(s) 169
ScrFI CCNGG 1 cut(s) 131
SetI ASST 6 cut(s) 53, 79, 181, 287, 612, 618
SfcI CTRYAG 2 cut(s) 164, 519
SinI GGWCC 1 cut(s) 375
SmiMI CAYNNNNRTG 2 cut(s) 357, 464
SmlI CTYRAG 2 cut(s) 215, 611
SmoI CTYRAG 2 cut(s) 215, 611
Sse9I AATT 2 cut(s) 316, 380
SsiI CCGC 3 cut(s) 41, 54, 87
StyD4I CCNGG 1 cut(s) 129
StyI CCWWGG 2 cut(s) 240, 261
TaaI ACNGT 1 cut(s) 509
TaqI TCGA 2 cut(s) 15, 158
TasI AATT 2 cut(s) 316, 380
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 1 cut(s) 645
Tru1I TTAA 2 cut(s) 119, 252
Tru9I TTAA 2 cut(s) 119, 252
TscAI CASTG 2 cut(s) 337, 377
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 1 cut(s) 594
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 1 cut(s) 454
TspRI CASTG 2 cut(s) 337, 377
VpaK11BI GGWCC 1 cut(s) 375
XapI RAATTY 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.