RchiOBHm_Chr3g0466001

Glucose-induced degradation protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0005099
Physical Location & Seq
Forward (+)
12594688 .. 12597216
2529 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43211

Sequence Viewer

Length: 621 bp
ATGGTAGTATGGTATATTCCGGTCAGAGCGGCAGAGACCTCCGCGCCTCCTCAACACCATTCAGGTTCAAACTGCGGACAGACATCTCCCCCACCCTTCACACTTTTAAGCGTTGGCCAGGGCTTCTCGGGTACTCAAAATGTTTCGAGTCTGCAGAAAGAAGAAGCTTGGAGAGTAAATGTTCGGATACAAGGGTGTGATCTTGAGAATGGTTATCTGTGTGGAACCATGGAAGCACTTAATGTTCCCATGGCTGACACACCAGTCGTCACCTTTTGGGAAGGGGAGATTGTTGACACCAAGAATTATACTTTCTTCACTGAGAAGTGGGAAGCAACACACGATGATGATATAAGGCACTGGACCAAATTTCCTTCTTTTTCTGCTCTTTTGAGCCGAGTGGAAGTTGATGGTGGCAAATCATTAGATCTCAGCAACTATAAATACATATTCATGAGATGGAAGGAGCAATACTTTGTGAATGTTGGCACAGACTGTGGTTTGACTATAGCCGGCTTTTACTATGTGTGTTTCTCATGTAGTGATGGCTCCATCAACGGCTTTTATTATGATCCTAATAGCAGCCCGTTCCAGAAGCTTGAGCTGAAAATCCACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vac_ImportDeg

Protein Analysis

206

Amino Acids

23.28

Weight (kDa)

5.17

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vac_ImportDeg PF09783 36 - 203 2.5e-51 Vacuolar import and degradation protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 263
AccBSI CCGCTC 1 cut(s) 29
AccII CGCG 1 cut(s) 44
AciI CCGC 3 cut(s) 29, 42, 75
AclWI GGATC 1 cut(s) 566
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 1 cut(s) 368
AfaI GTAC 1 cut(s) 133
AgsI TTSAA 1 cut(s) 69
AjnI CCWGG 1 cut(s) 117
AluBI AGCT 3 cut(s) 167, 598, 604
AluI AGCT 3 cut(s) 167, 598, 604
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 1 cut(s) 566
Ama87I CYCGRG 1 cut(s) 127
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 1 cut(s) 582
ApoI RAATTY 1 cut(s) 368
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 1 cut(s) 363
AsuHPI GGTGA 1 cut(s) 262
AvaI CYCGRG 1 cut(s) 127
AvaII GGWCC 1 cut(s) 363
BaeI ACNNNNGTAYC 2 cut(s) 179, 212
BalI TGGCCA 1 cut(s) 117
BarI GAAGNNNNNNTAC 2 cut(s) 455, 487
BbvI GCAGC 1 cut(s) 594
BccI CCATC 4 cut(s) 404, 453, 539, 560
BceAI ACGGC 1 cut(s) 574
BciT130I CCWGG 1 cut(s) 119
BciVI GTATCC 1 cut(s) 180
BcoDI GTCTC 1 cut(s) 29
BfmI CTRYAG 2 cut(s) 152, 507
BfuI GTATCC 1 cut(s) 180
BglII AGATCT 1 cut(s) 427
BisI GCNGC 2 cut(s) 30, 583
BlsI GCNGC 2 cut(s) 31, 584
Bme1390I CCNGG 1 cut(s) 119
Bme18I GGWCC 1 cut(s) 363
BmeT110I CYCGRG 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 363
BmiI GGNNCC 2 cut(s) 226, 550
BmrFI CCNGG 1 cut(s) 119
BpuEI CTTGAG 2 cut(s) 224, 620
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 3 cut(s) 118, 228, 249
BsaWI WCCGGW 1 cut(s) 19
Bse118I RCCGGY 1 cut(s) 512
Bse1I ACTGG 2 cut(s) 263, 365
BseBI CCWGG 1 cut(s) 119
BseDI CCNNGG 3 cut(s) 118, 228, 249
BseMII CTCAG 2 cut(s) 312, 445
BseNI ACTGG 2 cut(s) 263, 365
BseRI GAGGAG 1 cut(s) 39
BseXI GCAGC 1 cut(s) 594
Bsh1236I CGCG 1 cut(s) 44
BshFI GGCC 1 cut(s) 117
BsiHKCI CYCGRG 1 cut(s) 127
BsiSI CCGG 2 cut(s) 20, 513
BsmAI GTCTC 1 cut(s) 29
BsnI GGCC 1 cut(s) 117
Bso31I GGTCTC 1 cut(s) 29
BsoBI CYCGRG 1 cut(s) 127
Bsp143I GATC 3 cut(s) 199, 427, 571
Bsp19I CCATGG 2 cut(s) 228, 249
BspACI CCGC 3 cut(s) 29, 42, 75
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 2 cut(s) 313, 444
BspFNI CGCG 1 cut(s) 44
BspHI TCATGA 1 cut(s) 453
BspLI GGNNCC 2 cut(s) 226, 550
BspMAI CTGCAG 1 cut(s) 156
BspPI GGATC 1 cut(s) 566
BspTNI GGTCTC 1 cut(s) 29
BsrBI CCGCTC 1 cut(s) 29
BsrFI RCCGGY 1 cut(s) 512
BsrI ACTGG 2 cut(s) 263, 365
BssAI RCCGGY 1 cut(s) 512
BssECI CCNNGG 3 cut(s) 118, 228, 249
BssMI GATC 3 cut(s) 199, 427, 571
BssT1I CCWWGG 2 cut(s) 228, 249
Bst2UI CCWGG 1 cut(s) 119
Bst4CI ACNGT 1 cut(s) 497
BstC8I GCNNGC 1 cut(s) 514
BstDEI CTNAG 2 cut(s) 321, 431
BstDSI CCRYGG 2 cut(s) 228, 249
BstFNI CGCG 1 cut(s) 44
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 3 cut(s) 202, 430, 574
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 3 cut(s) 199, 427, 571
BstNI CCWGG 1 cut(s) 119
BstSCI CCNGG 1 cut(s) 117
BstSFI CTRYAG 2 cut(s) 152, 507
BstUI CGCG 1 cut(s) 44
BstV1I GCAGC 1 cut(s) 594
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
BsuI GTATCC 1 cut(s) 180
BsuRI GGCC 1 cut(s) 117
BtgI CCRYGG 2 cut(s) 228, 249
BtsIMutI CAGTG 2 cut(s) 318, 358
Cac8I GCNNGC 1 cut(s) 514
CciI TCATGA 1 cut(s) 453
CfoI GCGC 1 cut(s) 46
Cfr10I RCCGGY 1 cut(s) 512
Cfr13I GGNCC 1 cut(s) 363
Csp6I GTAC 1 cut(s) 132
CviAII CATG 4 cut(s) 229, 250, 454, 537
CviQI GTAC 1 cut(s) 132
DdeI CTNAG 2 cut(s) 321, 431
DpnI GATC 3 cut(s) 201, 429, 573
DpnII GATC 3 cut(s) 199, 427, 571
DrdI GACNNNNNNGTC 1 cut(s) 263
DseDI GACNNNNNNGTC 1 cut(s) 263
EaeI YGGCCR 1 cut(s) 115
Eco130I CCWWGG 2 cut(s) 228, 249
Eco31I GGTCTC 1 cut(s) 29
Eco47I GGWCC 1 cut(s) 363
Eco88I CYCGRG 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 117
EcoT14I CCWWGG 2 cut(s) 228, 249
ErhI CCWWGG 2 cut(s) 228, 249
FaeI CATG 4 cut(s) 232, 253, 457, 540
FatI CATG 4 cut(s) 228, 249, 453, 536
Fnu4HI GCNGC 2 cut(s) 30, 583
Fsp4HI GCNGC 2 cut(s) 30, 583
GlaI GCGC 1 cut(s) 45
GluI GCNGC 2 cut(s) 30, 583
HaeIII GGCC 1 cut(s) 117
HapII CCGG 2 cut(s) 20, 513
HhaI GCGC 1 cut(s) 46
Hin1II CATG 4 cut(s) 232, 253, 457, 540
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 2 cut(s) 165, 596
HinfI GANTC 1 cut(s) 148
HpaII CCGG 2 cut(s) 20, 513
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 2 cut(s) 26, 186
Hpy188III TCNNGA 3 cut(s) 203, 454, 592
Hpy8I GTNNAC 1 cut(s) 295
HpyAV CCTTC 4 cut(s) 106, 275, 384, 457
HpyCH4III ACNGT 1 cut(s) 497
HpyCH4V TGCA 1 cut(s) 154
HpyF3I CTNAG 2 cut(s) 321, 431
Hsp92II CATG 4 cut(s) 232, 253, 457, 540
HspAI GCGC 1 cut(s) 44
KroI GCCGGC 1 cut(s) 512
KroNI GCCGGC 1 cut(s) 514
Kzo9I GATC 3 cut(s) 199, 427, 571
LmnI GCTCC 2 cut(s) 466, 554
LpnPI CCDG 8 cut(s) 33, 48, 104, 131, 276, 346, 526, 605
Lsp1109I GCAGC 1 cut(s) 594
MaeIII GTNAC 1 cut(s) 268
MalI GATC 3 cut(s) 201, 429, 573
MbiI CCGCTC 1 cut(s) 29
MboI GATC 3 cut(s) 199, 427, 571
MboII GAAGA 2 cut(s) 173, 307
MflI RGATCY 1 cut(s) 427
MlsI TGGCCA 1 cut(s) 117
MluCI AATT 2 cut(s) 304, 368
MluNI TGGCCA 1 cut(s) 117
MlyI GAGTC 1 cut(s) 157
MnlI CCTC 3 cut(s) 49, 57, 60
Mox20I TGGCCA 1 cut(s) 117
MroNI GCCGGC 1 cut(s) 512
MscI TGGCCA 1 cut(s) 117
MseI TTAA 2 cut(s) 107, 240
MslI CAYNNNNRTG 2 cut(s) 345, 452
Msp20I TGGCCA 1 cut(s) 117
MspI CCGG 2 cut(s) 20, 513
MspR9I CCNGG 1 cut(s) 119
MvaI CCWGG 1 cut(s) 119
MvnI CGCG 1 cut(s) 44
NaeI GCCGGC 1 cut(s) 514
NcoI CCATGG 2 cut(s) 228, 249
NdeII GATC 3 cut(s) 199, 427, 571
NgoMIV GCCGGC 1 cut(s) 512
NlaIII CATG 4 cut(s) 232, 253, 457, 540
NlaIV GGNNCC 2 cut(s) 226, 550
NmeAIII GCCGAG 1 cut(s) 422
NmuCI GTSAC 1 cut(s) 268
PagI TCATGA 1 cut(s) 453
PdiI GCCGGC 1 cut(s) 514
PkrI GCNGC 2 cut(s) 31, 584
PleI GAGTC 1 cut(s) 156
PpsI GAGTC 1 cut(s) 156
Psp6I CCWGG 1 cut(s) 117
PspGI CCWGG 1 cut(s) 117
PspN4I GGNNCC 2 cut(s) 226, 550
PspPI GGNCC 1 cut(s) 363
PstI CTGCAG 1 cut(s) 156
PsuI RGATCY 1 cut(s) 427
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
RseI CAYNNNNRTG 2 cut(s) 345, 452
SaqAI TTAA 2 cut(s) 107, 240
SatI GCNGC 2 cut(s) 30, 583
Sau3AI GATC 3 cut(s) 199, 427, 571
Sau96I GGNCC 1 cut(s) 363
SchI GAGTC 1 cut(s) 157
ScrFI CCNGG 1 cut(s) 119
SetI ASST 6 cut(s) 41, 67, 169, 275, 600, 606
SfcI CTRYAG 2 cut(s) 152, 507
SinI GGWCC 1 cut(s) 363
SmiMI CAYNNNNRTG 2 cut(s) 345, 452
SmlI CTYRAG 2 cut(s) 203, 599
SmoI CTYRAG 2 cut(s) 203, 599
Sse9I AATT 2 cut(s) 304, 368
SsiI CCGC 3 cut(s) 29, 42, 75
StyD4I CCNGG 1 cut(s) 117
StyI CCWWGG 2 cut(s) 228, 249
TaaI ACNGT 1 cut(s) 497
TaqI TCGA 1 cut(s) 146
TasI AATT 2 cut(s) 304, 368
TauI GCSGC 1 cut(s) 32
Tru1I TTAA 2 cut(s) 107, 240
Tru9I TTAA 2 cut(s) 107, 240
TscAI CASTG 2 cut(s) 325, 365
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 1 cut(s) 582
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 1 cut(s) 442
TspRI CASTG 2 cut(s) 325, 365
VpaK11BI GGWCC 1 cut(s) 363
XapI RAATTY 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.