FvH4_6g20470

Belongs to the syntaxin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001517
Physical Location & Seq
Forward (+)
14105358 .. 14107508
2151 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g20470.t1

Sequence Viewer

Length: 882 bp
ATGAACGACTTGTTGTCCAACTCCTTCTCCCAGGCCTCGCCGGACCACCATGTCATCGAGATGACCTCCTTCGATGCCTCCAGCGACCTCAAGAAGTTCTTTGAGGACGTCGACTTGATCCAGACCGAGCTCAATGACTTGCAGAAGCTCAACACCAGCCTCAAGAGCGCGCACGAGCAGAGCAAGAGCCTCCACAAGGCCAAGGAGGTCAAGGACCTCCGGACCCGGATGGACGCTGACGTCAACCTTGCCCTTAAGGCGGTCAAGCTCCTTAAGGTTCGGTTGGAAACGCTGGACCGGTCCAACACCTCCGCCAGGAGCTTGCCTGGCTGCGGTCCGGGGTCGTCCTCGGACCGGACAAGGACGTCTGTGGTGAGTGGGTTGAGGAAGAAGCTTAAGGATTCTATGGAGAGCTTTAATGACTTGAGGCAGAAGATTTCCTCCGAGTACAAGGAGACCATTCAGAGAAGGTACTTTACTGTGACCGGAGAGAATCCGGACGACAAAACCATAGACCTTCTCATATCTACCGGCGAAAGTGAGACATTTTTGCAGAAGGCAATCCAACAGCAAGGAAGAGGCCAAGTCCTAGACACTATATACGAGATCCAGGAGAGGCACAATGGTGTGAAAGCCATGGAGAGGAATCTGAACGAGTTGCACCAGGTGTTTCTGGACATGGCAGTCCTGGTTCAAGCTCAGGGTGAGCAGATCGACAACATCGAGAGTCACGTAGATCGAGCTAGTTCGTTCGTGCAACACGGCAACCAGCAGTTGCAGAAGGCCAGATTCTTGCAGAAAAACACACGCAAATGGACCTGTTATGGTATCCTCATCTTGCTCATCATCATCGGTATCATATTAGCCTCCACATTGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000149 GO:0001101 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0006605 GO:0006612 GO:0006810 GO:0006833 GO:0006886 GO:0006887 GO:0006906 GO:0006950 GO:0006952 GO:0006996 GO:0008104 GO:0008150 GO:0009504 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010119 GO:0010148 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016043 GO:0016050 GO:0016192 GO:0022406 GO:0030054 GO:0031201 GO:0031224 GO:0031347 GO:0031348 GO:0031984 GO:0032507 GO:0032940 GO:0032991 GO:0033036 GO:0033993 GO:0034613 GO:0042044 GO:0042221 GO:0042886 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043495 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045185 GO:0046903 GO:0046907 GO:0048278 GO:0048284 GO:0048519 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051235 GO:0051640 GO:0051641 GO:0051649 GO:0051651 GO:0051704 GO:0051707 GO:0055044 GO:0061024 GO:0061025 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0072657 GO:0072658 GO:0072659 GO:0072660 GO:0080134 GO:0090150 GO:0090174 GO:0097305 GO:0098542 GO:0098791 GO:0098796 GO:0140056 GO:1901700 GO:1990778
KEGG Pathways
Metabolic & Signaling
ko04130 ko04721 map04130 map04721
Pfam Domains
Protein Families
SNARE Syntaxin

Protein Analysis

294

Amino Acids

33.15

Weight (kDa)

7.1

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syntaxin PF00804 28 - 188 2e-50 Syntaxin
SNARE PF05739 236 - 288 9.2e-20 SNARE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 4 cut(s) 50, 239, 364, 683
AatII GACGTC 3 cut(s) 111, 243, 368
AccI GTMKAC 1 cut(s) 111
AccII CGCG 1 cut(s) 170
AccIII TCCGGA 2 cut(s) 219, 496
AciI CCGC 3 cut(s) 260, 312, 333
AclWI GGATC 2 cut(s) 112, 601
AcyI GRCGYC 3 cut(s) 108, 240, 365
AdeI CACNNNGTG 1 cut(s) 667
AfaI GTAC 2 cut(s) 449, 473
AfiI CCNNNNNNNGG 6 cut(s) 196, 259, 315, 332, 354, 642
AflII CTTAAG 3 cut(s) 254, 272, 395
AgeI ACCGGT 1 cut(s) 297
AgsI TTSAA 1 cut(s) 695
AhdI GACNNNNNGTC 1 cut(s) 13
AjnI CCWGG 6 cut(s) 30, 314, 325, 609, 663, 687
AleI CACNNNNGTG 1 cut(s) 624
AluBI AGCT 8 cut(s) 130, 148, 268, 321, 394, 414, 698, 743
AluI AGCT 8 cut(s) 130, 148, 268, 321, 394, 414, 698, 743
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 2 cut(s) 449, 536
AlwI GGATC 2 cut(s) 112, 601
Aor13HI TCCGGA 2 cut(s) 219, 496
AoxI GGCC 4 cut(s) 33, 198, 580, 783
ApeKI GCWGC 1 cut(s) 330
AsiGI ACCGGT 1 cut(s) 297
AspLEI GCGC 2 cut(s) 170, 172
AspS9I GGNCC 8 cut(s) 43, 214, 222, 295, 300, 335, 352, 816
AsuC2I CCSGG 2 cut(s) 226, 339
AsuHPI GGTGA 2 cut(s) 385, 716
AvaII GGWCC 8 cut(s) 43, 214, 222, 295, 300, 335, 352, 816
BanII GRGCYC 1 cut(s) 132
BarI GAAGNNNNNNTAC 2 cut(s) 460, 492
BauI CACGAG 1 cut(s) 173
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 1 cut(s) 317
BccI CCATC 1 cut(s) 223
BceAI ACGGC 1 cut(s) 778
BciT130I CCWGG 6 cut(s) 32, 316, 327, 611, 665, 689
BciVI GTATCC 1 cut(s) 839
BcnI CCSGG 2 cut(s) 226, 339
BcoDI GTCTC 2 cut(s) 449, 536
BfaI CTAG 2 cut(s) 590, 744
BfrI CTTAAG 3 cut(s) 254, 272, 395
BfuI GTATCC 1 cut(s) 839
BglI GCCNNNNNGGC 1 cut(s) 257
BisI GCNGC 1 cut(s) 331
BlsI GCNGC 1 cut(s) 332
Bme1390I CCNGG 8 cut(s) 32, 226, 316, 327, 339, 611, 665, 689
Bme18I GGWCC 8 cut(s) 43, 214, 222, 295, 300, 335, 352, 816
BmeRI GACNNNNNGTC 1 cut(s) 13
BmgT120I GGNCC 8 cut(s) 43, 214, 222, 295, 300, 335, 352, 816
BmiI GGNNCC 1 cut(s) 224
BmrFI CCNGG 8 cut(s) 32, 226, 316, 327, 339, 611, 665, 689
BmsI GCATC 1 cut(s) 64
BplI GAGNNNNNCTC 2 cut(s) 50, 82
BpmI CTGGAG 1 cut(s) 64
Bpu10I CCTNAGC 1 cut(s) 699
BpuEI CTTGAG 3 cut(s) 74, 146, 445
BpuMI CCSGG 2 cut(s) 226, 339
BsaAI YACGTR 1 cut(s) 733
BsaHI GRCGYC 3 cut(s) 108, 240, 365
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 5 cut(s) 30, 201, 338, 348, 636
BsaWI WCCGGW 5 cut(s) 219, 297, 354, 485, 496
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 6 cut(s) 196, 259, 315, 332, 354, 642
Bse118I RCCGGY 2 cut(s) 297, 530
BseAI TCCGGA 2 cut(s) 219, 496
BseBI CCWGG 6 cut(s) 32, 316, 327, 611, 665, 689
BseDI CCNNGG 5 cut(s) 30, 201, 338, 348, 636
BseGI GGATG 1 cut(s) 234
BseLI CCNNNNNNNGG 6 cut(s) 196, 259, 315, 332, 354, 642
BseMII CTCAG 1 cut(s) 713
BsePI GCGCGC 1 cut(s) 168
BseXI GCAGC 1 cut(s) 317
Bsh1236I CGCG 1 cut(s) 170
BshFI GGCC 4 cut(s) 35, 200, 582, 785
BshTI ACCGGT 1 cut(s) 297
BsiHKAI GWGCWC 1 cut(s) 132
BsiSI CCGG 9 cut(s) 41, 220, 226, 298, 338, 355, 486, 497, 531
BslI CCNNNNNNNGG 6 cut(s) 196, 259, 315, 332, 354, 642
BsmAI GTCTC 2 cut(s) 449, 536
BsnI GGCC 4 cut(s) 35, 200, 582, 785
Bso31I GGTCTC 1 cut(s) 449
Bsp1286I GDGCHC 1 cut(s) 132
Bsp13I TCCGGA 2 cut(s) 219, 496
Bsp143I GATC 4 cut(s) 117, 606, 711, 736
Bsp19I CCATGG 1 cut(s) 636
BspACI CCGC 3 cut(s) 260, 312, 333
BspANI GGCC 4 cut(s) 35, 200, 582, 785
BspCNI CTCAG 1 cut(s) 712
BspEI TCCGGA 2 cut(s) 219, 496
BspFNI CGCG 1 cut(s) 170
BspLI GGNNCC 1 cut(s) 224
BspPI GGATC 2 cut(s) 112, 601
BspTI CTTAAG 3 cut(s) 254, 272, 395
BspTNI GGTCTC 1 cut(s) 449
BsrFI RCCGGY 2 cut(s) 297, 530
BssAI RCCGGY 2 cut(s) 297, 530
BssECI CCNNGG 5 cut(s) 30, 201, 338, 348, 636
BssHII GCGCGC 1 cut(s) 168
BssMI GATC 4 cut(s) 117, 606, 711, 736
BssNI GRCGYC 3 cut(s) 108, 240, 365
BssSI CACGAG 1 cut(s) 173
BssT1I CCWWGG 2 cut(s) 201, 636
Bst2BI CACGAG 1 cut(s) 173
Bst2UI CCWGG 6 cut(s) 32, 316, 327, 611, 665, 689
Bst4CI ACNGT 1 cut(s) 481
Bst6I CTCTTC 1 cut(s) 571
BstACI GRCGYC 3 cut(s) 108, 240, 365
BstAFI CTTAAG 3 cut(s) 254, 272, 395
BstBAI YACGTR 1 cut(s) 733
BstC8I GCNNGC 2 cut(s) 170, 323
BstDEI CTNAG 1 cut(s) 699
BstDSI CCRYGG 1 cut(s) 636
BstENI CCTNNNNNAGG 2 cut(s) 194, 313
BstF5I GGATG 1 cut(s) 234
BstFNI CGCG 1 cut(s) 170
BstHHI GCGC 2 cut(s) 170, 172
BstKTI GATC 4 cut(s) 120, 609, 714, 739
BstMAI GTCTC 2 cut(s) 449, 536
BstMBI GATC 4 cut(s) 117, 606, 711, 736
BstMWI GCNNNNNNNGC 3 cut(s) 165, 257, 327
BstNI CCWGG 6 cut(s) 32, 316, 327, 611, 665, 689
BstSCI CCNGG 8 cut(s) 30, 224, 314, 325, 337, 609, 663, 687
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 1 cut(s) 317
BstX2I RGATCY 1 cut(s) 606
BstYI RGATCY 1 cut(s) 606
BsuI GTATCC 1 cut(s) 839
BsuRI GGCC 4 cut(s) 35, 200, 582, 785
BtgI CCRYGG 1 cut(s) 636
BtsCI GGATG 1 cut(s) 234
Cac8I GCNNGC 2 cut(s) 170, 323
CfoI GCGC 2 cut(s) 170, 172
Cfr10I RCCGGY 2 cut(s) 297, 530
Cfr13I GGNCC 8 cut(s) 43, 214, 222, 295, 300, 335, 352, 816
CpoI CGGWCCG 2 cut(s) 335, 352
CseI GACGC 1 cut(s) 242
CsiI ACCWGGT 1 cut(s) 663
Csp6I GTAC 2 cut(s) 448, 472
CspAI ACCGGT 1 cut(s) 297
CspI CGGWCCG 2 cut(s) 335, 352
CviAII CATG 3 cut(s) 50, 637, 679
CviQI GTAC 2 cut(s) 448, 472
DdeI CTNAG 1 cut(s) 699
DpnI GATC 4 cut(s) 119, 608, 713, 738
DpnII GATC 4 cut(s) 117, 606, 711, 736
DraIII CACNNNGTG 1 cut(s) 667
DrdI GACNNNNNNGTC 4 cut(s) 50, 239, 364, 683
DriI GACNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 4 cut(s) 50, 239, 364, 683
Eam1104I CTCTTC 1 cut(s) 571
Eam1105I GACNNNNNGTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 571
EciI GGCGGA 1 cut(s) 301
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 2 cut(s) 201, 636
Eco147I AGGCCT 1 cut(s) 35
Eco24I GRGCYC 1 cut(s) 132
Eco31I GGTCTC 1 cut(s) 449
Eco47I GGWCC 8 cut(s) 43, 214, 222, 295, 300, 335, 352, 816
Eco53kI GAGCTC 1 cut(s) 130
EcoICRI GAGCTC 1 cut(s) 130
EcoNI CCTNNNNNAGG 2 cut(s) 194, 313
EcoO109I RGGNCCY 1 cut(s) 214
EcoRII CCWGG 6 cut(s) 30, 314, 325, 609, 663, 687
EcoT14I CCWWGG 2 cut(s) 201, 636
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 2 cut(s) 201, 636
FaeI CATG 3 cut(s) 53, 640, 682
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FatI CATG 3 cut(s) 49, 636, 678
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 1 cut(s) 331
FokI GGATG 1 cut(s) 241
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 1 cut(s) 331
FspBI CTAG 2 cut(s) 590, 744
GlaI GCGC 2 cut(s) 169, 171
GluI GCNGC 1 cut(s) 331
GsuI CTGGAG 1 cut(s) 64
HaeIII GGCC 4 cut(s) 35, 200, 582, 785
HapII CCGG 9 cut(s) 41, 220, 226, 298, 338, 355, 486, 497, 531
HgaI GACGC 1 cut(s) 242
HhaI GCGC 2 cut(s) 170, 172
Hin1I GRCGYC 3 cut(s) 108, 240, 365
Hin1II CATG 3 cut(s) 53, 640, 682
Hin6I GCGC 2 cut(s) 168, 170
HinP1I GCGC 2 cut(s) 168, 170
HincII GTYRAC 2 cut(s) 112, 244
HindII GTYRAC 2 cut(s) 112, 244
HindIII AAGCTT 1 cut(s) 392
HinfI GANTC 5 cut(s) 401, 493, 646, 727, 789
HpaII CCGG 9 cut(s) 41, 220, 226, 298, 338, 355, 486, 497, 531
HphI GGTGA 2 cut(s) 385, 716
Hpy166II GTNNAC 2 cut(s) 112, 244
Hpy188I TCNGA 4 cut(s) 352, 445, 465, 651
Hpy188III TCNNGA 8 cut(s) 58, 91, 121, 163, 220, 497, 674, 724
Hpy8I GTNNAC 2 cut(s) 112, 244
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 6 cut(s) 34, 79, 462, 527, 550, 775
HpyCH4III ACNGT 1 cut(s) 481
HpyCH4IV ACGT 4 cut(s) 108, 240, 365, 732
HpyCH4V TGCA 6 cut(s) 142, 553, 661, 757, 778, 796
HpyF10VI GCNNNNNNNGC 3 cut(s) 165, 257, 327
HpyF3I CTNAG 1 cut(s) 699
HpySE526I ACGT 4 cut(s) 108, 240, 365, 732
Hsp92I GRCGYC 3 cut(s) 108, 240, 365
Hsp92II CATG 3 cut(s) 53, 640, 682
HspAI GCGC 2 cut(s) 168, 170
Kpn2I TCCGGA 2 cut(s) 219, 496
Kzo9I GATC 4 cut(s) 117, 606, 711, 736
LmnI GCTCC 2 cut(s) 273, 318
Lsp1109I GCAGC 1 cut(s) 317
LweI GCATC 1 cut(s) 64
MabI ACCWGGT 1 cut(s) 663
MaeI CTAG 2 cut(s) 590, 744
MaeII ACGT 4 cut(s) 108, 240, 365, 732
MaeIII GTNAC 2 cut(s) 481, 728
MalI GATC 4 cut(s) 119, 608, 713, 738
MboI GATC 4 cut(s) 117, 606, 711, 736
MboII GAAGA 3 cut(s) 400, 445, 588
MflI RGATCY 1 cut(s) 606
MhlI GDGCHC 1 cut(s) 132
MlyI GAGTC 1 cut(s) 736
MmeI TCCRAC 4 cut(s) 42, 264, 327, 589
MroI TCCGGA 2 cut(s) 219, 496
MseI TTAA 5 cut(s) 255, 273, 396, 417, 880
MslI CAYNNNNRTG 3 cut(s) 59, 624, 811
MspCI CTTAAG 3 cut(s) 254, 272, 395
MspI CCGG 9 cut(s) 41, 220, 226, 298, 338, 355, 486, 497, 531
MspR9I CCNGG 8 cut(s) 32, 226, 316, 327, 339, 611, 665, 689
MvaI CCWGG 6 cut(s) 32, 316, 327, 611, 665, 689
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 3 cut(s) 165, 257, 327
NciI CCSGG 2 cut(s) 226, 339
NcoI CCATGG 1 cut(s) 636
NdeII GATC 4 cut(s) 117, 606, 711, 736
NlaIII CATG 3 cut(s) 53, 640, 682
NlaIV GGNNCC 1 cut(s) 224
NmuCI GTSAC 2 cut(s) 481, 728
OliI CACNNNNGTG 1 cut(s) 624
PauI GCGCGC 1 cut(s) 168
PceI AGGCCT 1 cut(s) 35
PcsI WCGNNNNNNNCGW 2 cut(s) 720, 729
PfeI GAWTC 4 cut(s) 401, 493, 646, 789
PfoI TCCNGGA 1 cut(s) 609
PinAI ACCGGT 1 cut(s) 297
PkrI GCNGC 1 cut(s) 332
PleI GAGTC 1 cut(s) 735
PpsI GAGTC 1 cut(s) 735
Ppu21I YACGTR 1 cut(s) 733
PpuMI RGGWCCY 1 cut(s) 214
Psp124BI GAGCTC 1 cut(s) 132
Psp5II RGGWCCY 1 cut(s) 214
Psp6I CCWGG 6 cut(s) 30, 314, 325, 609, 663, 687
PspGI CCWGG 6 cut(s) 30, 314, 325, 609, 663, 687
PspN4I GGNNCC 1 cut(s) 224
PspPI GGNCC 8 cut(s) 43, 214, 222, 295, 300, 335, 352, 816
PspPPI RGGWCCY 1 cut(s) 214
PsuI RGATCY 1 cut(s) 606
PteI GCGCGC 1 cut(s) 168
RsaI GTAC 2 cut(s) 449, 473
RsaNI GTAC 2 cut(s) 448, 472
RseI CAYNNNNRTG 3 cut(s) 59, 624, 811
Rsr2I CGGWCCG 2 cut(s) 335, 352
RsrII CGGWCCG 2 cut(s) 335, 352
SacI GAGCTC 1 cut(s) 132
SalI GTCGAC 1 cut(s) 110
SaqAI TTAA 5 cut(s) 255, 273, 396, 417, 880
SatI GCNGC 1 cut(s) 331
Sau3AI GATC 4 cut(s) 117, 606, 711, 736
Sau96I GGNCC 8 cut(s) 43, 214, 222, 295, 300, 335, 352, 816
SchI GAGTC 1 cut(s) 736
ScrFI CCNGG 8 cut(s) 32, 226, 316, 327, 339, 611, 665, 689
SduI GDGCHC 1 cut(s) 132
SexAI ACCWGGT 1 cut(s) 663
SfaNI GCATC 1 cut(s) 64
SinI GGWCC 8 cut(s) 43, 214, 222, 295, 300, 335, 352, 816
SmiMI CAYNNNNRTG 3 cut(s) 59, 624, 811
SmlI CTYRAG 6 cut(s) 89, 161, 254, 272, 395, 424
SmoI CTYRAG 6 cut(s) 89, 161, 254, 272, 395, 424
SseBI AGGCCT 1 cut(s) 35
SsiI CCGC 3 cut(s) 260, 312, 333
SspMI CTAG 2 cut(s) 590, 744
SstI GAGCTC 1 cut(s) 132
StuI AGGCCT 1 cut(s) 35
StyD4I CCNGG 8 cut(s) 30, 224, 314, 325, 337, 609, 663, 687
StyI CCWWGG 2 cut(s) 201, 636
TaaI ACNGT 1 cut(s) 481
TaiI ACGT 4 cut(s) 111, 243, 368, 735
TaqI TCGA 6 cut(s) 57, 72, 111, 714, 723, 739
TaqII GACCGA 1 cut(s) 140
TatI WGTACW 1 cut(s) 447
TfiI GAWTC 4 cut(s) 401, 493, 646, 789
Tru1I TTAA 5 cut(s) 255, 273, 396, 417, 880
Tru9I TTAA 5 cut(s) 255, 273, 396, 417, 880
TseFI GTSAC 2 cut(s) 481, 728
TseI GCWGC 1 cut(s) 330
Tsp45I GTSAC 2 cut(s) 481, 728
TspDTI ATGAA 1 cut(s) 17
Vha464I CTTAAG 3 cut(s) 254, 272, 395
VpaK11BI GGWCC 8 cut(s) 43, 214, 222, 295, 300, 335, 352, 816
XagI CCTNNNNNAGG 2 cut(s) 194, 313
XmiI GTMKAC 1 cut(s) 111
XspI CTAG 2 cut(s) 590, 744
ZraI GACGTC 3 cut(s) 109, 241, 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.