MD12G1041700.v1.1

Belongs to the syntaxin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001517
Physical Location & Seq
Forward (+)
4632174 .. 4633930
1757 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1041700.v1.1.491

Sequence Viewer

Length: 1005 bp
ATGAACGATTTGTTCTCCGGCTCCTTCTCGCGGTTCCGGAGCGAGGAGCAGTCGCCGCGGCGCGATGATCACGTGATCGAGATGGGGTCGACGGCGGCGGCGAGGTCGGGCACCGCCGGAGTCAACCTCGACAAGTTCTTCGCCGACGTCGAGTCCGTCAAGGACGAGCTCAAGGAGCTCGAGCGCGTGTACTTCAGCCTCCAGTCCTCCCACGAGCAGAGCAAGACGCTCCACAACGCCTCCGCCGTCAAGGACCTCCGATCCCGCATGGACGCTGACGTCACTCTCGCTCTGAAGAAGGCCAAGGTGCTGAAGGTCCGCCTCGAGGCGCTAGACCGATCCAACGCCGCGAACCGGAGCCTGCCGAACTGCGGGCCGGGGTCGTCGTCGGACCGGACGCGGATCTCCGTCGTCAACGGCCTCCGCAAGAAGCTCAAGGACTCCATGGATAGCTTCAACGCTCTGCGGCAGAAGATCTCGTCCGAGTACCGCGAGACCGTACAGCGGAGGTACTACACCGTCTCCGGCCAAAATCCCGACGAGAAAACATTAGACCTCCTCATCTCCACCGGAGAGAGCGAAACGTTTCTGCAGAAGGCAATACAGGAGCAAGGGAGGGGCCAAGTCCTTGACACGATTCAGGAGATCCAGGAGCGACACGACGCCGTAAAGGACATGGAGAGGAACCTGCAGGAGCTGCACCAGGTGTTCCTGGACATGGCCGTGCTGGTGCAGGCGCAGGGGGAGCAGATCGACGACATCGAAAACCACGTCATGAGAGCCAACTCGTTCGTCAACAGTGGCACCCAGCAGCTCACCAAGGCCAGGGCCTCCCAGAAGAACACCCGCAAGTGGTCTTGCTTCGCCATTATTCTGCTGCTGGTTATTATTCTGATCGTGATCCTCAGCTTGAAGCCCTGGAATTGGGGTGGCGGTAGCAATAACAACAATAATAATATCAATAATCCGACGCCCGCCGCGCCACCGCCACCGCAACCGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000149 GO:0001101 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0006605 GO:0006612 GO:0006810 GO:0006833 GO:0006886 GO:0006887 GO:0006906 GO:0006950 GO:0006952 GO:0006996 GO:0008104 GO:0008150 GO:0009504 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010119 GO:0010148 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016043 GO:0016050 GO:0016192 GO:0022406 GO:0030054 GO:0031201 GO:0031224 GO:0031347 GO:0031348 GO:0031984 GO:0032507 GO:0032940 GO:0032991 GO:0033036 GO:0033993 GO:0034613 GO:0042044 GO:0042221 GO:0042886 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043495 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045185 GO:0046903 GO:0046907 GO:0048278 GO:0048284 GO:0048519 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051235 GO:0051640 GO:0051641 GO:0051649 GO:0051651 GO:0051704 GO:0051707 GO:0055044 GO:0061024 GO:0061025 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0072657 GO:0072658 GO:0072659 GO:0072660 GO:0080134 GO:0090150 GO:0090174 GO:0097305 GO:0098542 GO:0098791 GO:0098796 GO:0140056 GO:1901700 GO:1990778
KEGG Pathways
Metabolic & Signaling
ko04130 ko04721 map04130 map04721
Pfam Domains
Protein Families
SNARE Syntaxin

Protein Analysis

335

Amino Acids

37.47

Weight (kDa)

8.43

Isoelectric Point (pI)

50.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syntaxin PF00804 42 - 201 3.5e-51 Syntaxin
SNARE PF05739 249 - 301 2.4e-18 SNARE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 278
AatII GACGTC 2 cut(s) 150, 282
AbsI CCTCGAGG 1 cut(s) 323
Acc36I ACCTGC 1 cut(s) 696
AccB1I GGYRCC 2 cut(s) 110, 803
AccI GTMKAC 1 cut(s) 89
AccII CGCG 8 cut(s) 31, 58, 63, 186, 350, 400, 492, 980
AccIII TCCGGA 1 cut(s) 36
AclI AACGTT 1 cut(s) 584
AclWI GGATC 5 cut(s) 255, 333, 410, 640, 895
AcoI YGGCCR 2 cut(s) 526, 720
AcuI CTGAAG 3 cut(s) 178, 314, 332
AcvI CACGTG 1 cut(s) 73
AcyI GRCGYC 4 cut(s) 147, 279, 663, 971
AdeI CACNNNGTG 1 cut(s) 706
AfaI GTAC 4 cut(s) 191, 488, 501, 512
AgsI TTSAA 2 cut(s) 457, 913
AhdI GACNNNNNGTC 1 cut(s) 151
AjiI CACGTC 1 cut(s) 772
AjnI CCWGG 5 cut(s) 648, 702, 711, 824, 917
AluBI AGCT 7 cut(s) 169, 178, 433, 453, 697, 814, 909
AluI AGCT 7 cut(s) 169, 178, 433, 453, 697, 814, 909
Alw21I GWGCWC 2 cut(s) 171, 180
Alw26I GTCTC 2 cut(s) 488, 526
AlwI GGATC 5 cut(s) 255, 333, 410, 640, 895
AlwNI CAGNNNCTG 1 cut(s) 697
Ama87I CYCGRG 2 cut(s) 179, 323
Aor13HI TCCGGA 1 cut(s) 36
AoxI GGCC 8 cut(s) 300, 374, 418, 526, 619, 720, 822, 828
ApeKI GCWGC 3 cut(s) 697, 811, 877
ArsI GACNNNNNNTTYG 4 cut(s) 122, 154, 756, 788
Asp700I GAANNNNTTC 1 cut(s) 585
AspLEI GCGC 5 cut(s) 63, 186, 331, 739, 982
AspS9I GGNCC 6 cut(s) 253, 316, 374, 391, 619, 828
AsuC2I CCSGG 1 cut(s) 378
AsuHPI GGTGA 1 cut(s) 808
AvaI CYCGRG 2 cut(s) 179, 323
AvaII GGWCC 3 cut(s) 253, 316, 391
BaeGI GKGCMC 1 cut(s) 113
BanI GGYRCC 2 cut(s) 110, 803
BanII GRGCYC 2 cut(s) 171, 180
BauI CACGAG 1 cut(s) 212
BbrPI CACGTG 1 cut(s) 73
Bbv12I GWGCWC 2 cut(s) 171, 180
BbvCI CCTCAGC 1 cut(s) 905
BbvI GCAGC 3 cut(s) 684, 823, 864
BccI CCATC 1 cut(s) 76
BceAI ACGGC 5 cut(s) 108, 230, 433, 650, 707
BciT130I CCWGG 5 cut(s) 650, 704, 713, 826, 919
BclI TGATCA 1 cut(s) 67
BcnI CCSGG 1 cut(s) 378
BcoDI GTCTC 2 cut(s) 488, 526
BfaI CTAG 1 cut(s) 332
BfmI CTRYAG 2 cut(s) 590, 689
BfoI RGCGCY 1 cut(s) 332
BfuAI ACCTGC 1 cut(s) 696
BglII AGATCT 1 cut(s) 474
Bme1390I CCNGG 6 cut(s) 378, 650, 704, 713, 826, 919
Bme18I GGWCC 3 cut(s) 253, 316, 391
BmeRI GACNNNNNGTC 1 cut(s) 151
BmeT110I CYCGRG 2 cut(s) 179, 323
BmgBI CACGTC 1 cut(s) 772
BmgT120I GGNCC 6 cut(s) 253, 316, 374, 391, 619, 828
BmiI GGNNCC 7 cut(s) 22, 35, 112, 359, 620, 686, 805
BmrFI CCNGG 6 cut(s) 378, 650, 704, 713, 826, 919
BplI GAGNNNNNCTC 2 cut(s) 111, 143
BpmI CTGGAG 1 cut(s) 185
Bpu10I CCTNAGC 1 cut(s) 905
BpuEI CTTGAG 2 cut(s) 155, 419
BpuMI CCSGG 1 cut(s) 378
BsaAI YACGTR 1 cut(s) 73
BsaBI GATNNNNATC 1 cut(s) 899
BsaHI GRCGYC 4 cut(s) 147, 279, 663, 971
BsaI GGTCTC 1 cut(s) 488
BsaJI CCNNGG 7 cut(s) 56, 303, 377, 444, 819, 825, 917
BsaWI WCCGGW 4 cut(s) 36, 354, 393, 569
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 506, 536
Bse1I ACTGG 1 cut(s) 202
Bse8I GATNNNNATC 1 cut(s) 899
BseAI TCCGGA 1 cut(s) 36
BseBI CCWGG 5 cut(s) 650, 704, 713, 826, 919
BseDI CCNNGG 7 cut(s) 56, 303, 377, 444, 819, 825, 917
BseJI GATNNNNATC 1 cut(s) 899
BseMII CTCAG 1 cut(s) 919
BseNI ACTGG 1 cut(s) 202
BseRI GAGGAG 2 cut(s) 59, 548
BseSI GKGCMC 1 cut(s) 113
BseXI GCAGC 3 cut(s) 684, 823, 864
BseYI CCCAGC 1 cut(s) 807
BsgI GTGCAG 2 cut(s) 683, 752
Bsh1236I CGCG 8 cut(s) 31, 58, 63, 186, 350, 400, 492, 980
BshFI GGCC 8 cut(s) 302, 376, 420, 528, 621, 722, 824, 830
BshNI GGYRCC 2 cut(s) 110, 803
BsiHKAI GWGCWC 2 cut(s) 171, 180
BsiHKCI CYCGRG 2 cut(s) 179, 323
BsiSI CCGG 8 cut(s) 18, 37, 117, 355, 377, 394, 525, 570
BsmAI GTCTC 2 cut(s) 488, 526
BsmBI CGTCTC 1 cut(s) 526
BsnI GGCC 8 cut(s) 302, 376, 420, 528, 621, 722, 824, 830
Bso31I GGTCTC 1 cut(s) 488
BsoBI CYCGRG 2 cut(s) 179, 323
Bsp1286I GDGCHC 3 cut(s) 113, 171, 180
Bsp13I TCCGGA 1 cut(s) 36
Bsp19I CCATGG 1 cut(s) 444
BspANI GGCC 8 cut(s) 302, 376, 420, 528, 621, 722, 824, 830
BspCNI CTCAG 1 cut(s) 918
BspEI TCCGGA 1 cut(s) 36
BspFNI CGCG 8 cut(s) 31, 58, 63, 186, 350, 400, 492, 980
BspHI TCATGA 1 cut(s) 774
BspLI GGNNCC 7 cut(s) 22, 35, 112, 359, 620, 686, 805
BspMAI CTGCAG 2 cut(s) 594, 693
BspMI ACCTGC 1 cut(s) 696
BspPI GGATC 5 cut(s) 255, 333, 410, 640, 895
BspT107I GGYRCC 2 cut(s) 110, 803
BspTNI GGTCTC 1 cut(s) 488
BsrI ACTGG 1 cut(s) 202
BssECI CCNNGG 7 cut(s) 56, 303, 377, 444, 819, 825, 917
BssNI GRCGYC 4 cut(s) 147, 279, 663, 971
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 3 cut(s) 303, 444, 819
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 5 cut(s) 650, 704, 713, 826, 919
Bst4CI ACNGT 3 cut(s) 499, 520, 800
BstACI GRCGYC 4 cut(s) 147, 279, 663, 971
BstAPI GCANNNNNTGC 1 cut(s) 697
BstBAI YACGTR 1 cut(s) 73
BstC8I GCNNGC 4 cut(s) 362, 374, 735, 975
BstDEI CTNAG 1 cut(s) 905
BstDSI CCRYGG 2 cut(s) 56, 444
BstFNI CGCG 8 cut(s) 31, 58, 63, 186, 350, 400, 492, 980
BstH2I RGCGCY 1 cut(s) 332
BstHHI GCGC 5 cut(s) 63, 186, 331, 739, 982
BstMAI GTCTC 2 cut(s) 488, 526
BstMWI GCNNNNNNNGC 5 cut(s) 55, 175, 697, 745, 979
BstNI CCWGG 5 cut(s) 650, 704, 713, 826, 919
BstSCI CCNGG 6 cut(s) 376, 648, 702, 711, 824, 917
BstSFI CTRYAG 2 cut(s) 590, 689
BstSLI GKGCMC 1 cut(s) 113
BstUI CGCG 8 cut(s) 31, 58, 63, 186, 350, 400, 492, 980
BstV1I GCAGC 3 cut(s) 684, 823, 864
BstX2I RGATCY 3 cut(s) 402, 474, 645
BstYI RGATCY 3 cut(s) 402, 474, 645
BsuRI GGCC 8 cut(s) 302, 376, 420, 528, 621, 722, 824, 830
BtgI CCRYGG 2 cut(s) 56, 444
BtgZI GCGATG 1 cut(s) 78
BtrI CACGTC 1 cut(s) 772
BtsIMutI CAGTG 1 cut(s) 805
BveI ACCTGC 1 cut(s) 696
Cac8I GCNNGC 4 cut(s) 362, 374, 735, 975
CaiI CAGNNNCTG 1 cut(s) 697
CciI TCATGA 1 cut(s) 774
CfoI GCGC 5 cut(s) 63, 186, 331, 739, 982
Cfr13I GGNCC 6 cut(s) 253, 316, 374, 391, 619, 828
Cfr42I CCGCGG 1 cut(s) 59
CpoI CGGWCCG 1 cut(s) 391
CseI GACGC 5 cut(s) 235, 281, 406, 671, 979
CsiI ACCWGGT 1 cut(s) 702
Csp6I GTAC 4 cut(s) 190, 487, 500, 511
CspI CGGWCCG 1 cut(s) 391
CviAII CATG 5 cut(s) 268, 445, 676, 718, 775
CviQI GTAC 4 cut(s) 190, 487, 500, 511
DdeI CTNAG 1 cut(s) 905
DraIII CACNNNGTG 1 cut(s) 706
DrdI GACNNNNNNGTC 1 cut(s) 278
DriI GACNNNNNGTC 1 cut(s) 151
DseDI GACNNNNNNGTC 1 cut(s) 278
EaeI YGGCCR 2 cut(s) 526, 720
Eam1105I GACNNNNNGTC 1 cut(s) 151
EciI GGCGGA 2 cut(s) 232, 308
Ecl136II GAGCTC 2 cut(s) 169, 178
Eco130I CCWWGG 3 cut(s) 303, 444, 819
Eco24I GRGCYC 2 cut(s) 171, 180
Eco31I GGTCTC 1 cut(s) 488
Eco47I GGWCC 3 cut(s) 253, 316, 391
Eco53kI GAGCTC 2 cut(s) 169, 178
Eco57I CTGAAG 3 cut(s) 178, 314, 332
Eco72I CACGTG 1 cut(s) 73
Eco88I CYCGRG 2 cut(s) 179, 323
EcoICRI GAGCTC 2 cut(s) 169, 178
EcoO109I RGGNCCY 2 cut(s) 253, 828
EcoRII CCWGG 5 cut(s) 648, 702, 711, 824, 917
EcoT14I CCWWGG 3 cut(s) 303, 444, 819
EcoT38I GRGCYC 2 cut(s) 171, 180
ErhI CCWWGG 3 cut(s) 303, 444, 819
Esp3I CGTCTC 1 cut(s) 526
FaeI CATG 5 cut(s) 271, 448, 679, 721, 778
FaiI YATR 5 cut(s) 269, 446, 677, 719, 776
FatI CATG 5 cut(s) 267, 444, 675, 717, 774
FauI CCCGC 4 cut(s) 272, 365, 854, 982
FbaI TGATCA 1 cut(s) 67
FblI GTMKAC 1 cut(s) 89
FriOI GRGCYC 2 cut(s) 171, 180
FspBI CTAG 1 cut(s) 332
GlaI GCGC 5 cut(s) 62, 185, 330, 738, 981
GsaI CCCAGC 1 cut(s) 811
GsuI CTGGAG 1 cut(s) 185
HaeII RGCGCY 1 cut(s) 332
HaeIII GGCC 8 cut(s) 302, 376, 420, 528, 621, 722, 824, 830
HapII CCGG 8 cut(s) 18, 37, 117, 355, 377, 394, 525, 570
HgaI GACGC 5 cut(s) 235, 281, 406, 671, 979
HhaI GCGC 5 cut(s) 63, 186, 331, 739, 982
Hin1I GRCGYC 4 cut(s) 147, 279, 663, 971
Hin1II CATG 5 cut(s) 271, 448, 679, 721, 778
Hin6I GCGC 5 cut(s) 61, 184, 329, 737, 980
HinP1I GCGC 5 cut(s) 61, 184, 329, 737, 980
HincII GTYRAC 4 cut(s) 90, 124, 415, 796
HindII GTYRAC 4 cut(s) 90, 124, 415, 796
HinfI GANTC 4 cut(s) 120, 152, 440, 637
HpaII CCGG 8 cut(s) 18, 37, 117, 355, 377, 394, 525, 570
HphI GGTGA 1 cut(s) 808
Hpy166II GTNNAC 5 cut(s) 90, 124, 190, 415, 796
Hpy188I TCNGA 6 cut(s) 260, 294, 391, 484, 894, 969
Hpy188III TCNNGA 6 cut(s) 37, 79, 536, 641, 775, 898
Hpy8I GTNNAC 5 cut(s) 90, 124, 190, 415, 796
HpyAV CCTTC 4 cut(s) 34, 292, 307, 589
HpyCH4III ACNGT 3 cut(s) 499, 520, 800
HpyCH4IV ACGT 5 cut(s) 72, 147, 279, 584, 771
HpyCH4V TGCA 4 cut(s) 592, 691, 700, 733
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 175, 697, 745, 979
HpyF3I CTNAG 1 cut(s) 905
HpySE526I ACGT 5 cut(s) 72, 147, 279, 584, 771
Hsp92I GRCGYC 4 cut(s) 147, 279, 663, 971
Hsp92II CATG 5 cut(s) 271, 448, 679, 721, 778
HspAI GCGC 5 cut(s) 61, 184, 329, 737, 980
Kpn2I TCCGGA 1 cut(s) 36
Ksp22I TGATCA 1 cut(s) 67
KspI CCGCGG 1 cut(s) 59
Lsp1109I GCAGC 3 cut(s) 684, 823, 864
MabI ACCWGGT 1 cut(s) 702
MaeI CTAG 1 cut(s) 332
MaeII ACGT 5 cut(s) 72, 147, 279, 584, 771
MaeIII GTNAC 1 cut(s) 280
MboII GAAGA 4 cut(s) 130, 307, 484, 850
MflI RGATCY 3 cut(s) 402, 474, 645
MhlI GDGCHC 3 cut(s) 113, 171, 180
MluCI AATT 1 cut(s) 922
MlyI GAGTC 3 cut(s) 129, 161, 434
MmeI TCCRAC 3 cut(s) 366, 369, 992
MroI TCCGGA 1 cut(s) 36
MroXI GAANNNNTTC 1 cut(s) 585
MslI CAYNNNNRTG 1 cut(s) 722
MspA1I CMGCKG 2 cut(s) 58, 505
MspI CCGG 8 cut(s) 18, 37, 117, 355, 377, 394, 525, 570
MspR9I CCNGG 6 cut(s) 378, 650, 704, 713, 826, 919
MvaI CCWGG 5 cut(s) 650, 704, 713, 826, 919
MvnI CGCG 8 cut(s) 31, 58, 63, 186, 350, 400, 492, 980
MwoI GCNNNNNNNGC 5 cut(s) 55, 175, 697, 745, 979
NciI CCSGG 1 cut(s) 378
NcoI CCATGG 1 cut(s) 444
NlaIII CATG 5 cut(s) 271, 448, 679, 721, 778
NlaIV GGNNCC 7 cut(s) 22, 35, 112, 359, 620, 686, 805
NmuCI GTSAC 1 cut(s) 280
PaeR7I CTCGAG 2 cut(s) 179, 323
PagI TCATGA 1 cut(s) 774
PcsI WCGNNNNNNNCGW 6 cut(s) 98, 147, 153, 243, 759, 768
PdmI GAANNNNTTC 1 cut(s) 585
PfeI GAWTC 1 cut(s) 637
PfoI TCCNGGA 2 cut(s) 648, 711
PleI GAGTC 3 cut(s) 128, 160, 434
PmaCI CACGTG 1 cut(s) 73
PmlI CACGTG 1 cut(s) 73
PpsI GAGTC 3 cut(s) 128, 160, 434
Ppu21I YACGTR 1 cut(s) 73
PpuMI RGGWCCY 1 cut(s) 253
Psp124BI GAGCTC 2 cut(s) 171, 180
Psp1406I AACGTT 1 cut(s) 584
Psp5II RGGWCCY 1 cut(s) 253
Psp6I CCWGG 5 cut(s) 648, 702, 711, 824, 917
PspCI CACGTG 1 cut(s) 73
PspFI CCCAGC 1 cut(s) 807
PspGI CCWGG 5 cut(s) 648, 702, 711, 824, 917
PspN4I GGNNCC 7 cut(s) 22, 35, 112, 359, 620, 686, 805
PspPI GGNCC 6 cut(s) 253, 316, 374, 391, 619, 828
PspPPI RGGWCCY 1 cut(s) 253
PspXI VCTCGAGB 2 cut(s) 179, 323
PstI CTGCAG 2 cut(s) 594, 693
PstNI CAGNNNCTG 1 cut(s) 697
PsuI RGATCY 3 cut(s) 402, 474, 645
RsaI GTAC 4 cut(s) 191, 488, 501, 512
RsaNI GTAC 4 cut(s) 190, 487, 500, 511
RseI CAYNNNNRTG 1 cut(s) 722
Rsr2I CGGWCCG 1 cut(s) 391
RsrII CGGWCCG 1 cut(s) 391
SacI GAGCTC 2 cut(s) 171, 180
SacII CCGCGG 1 cut(s) 59
SalI GTCGAC 1 cut(s) 88
Sau96I GGNCC 6 cut(s) 253, 316, 374, 391, 619, 828
SbfI CCTGCAGG 1 cut(s) 693
SchI GAGTC 3 cut(s) 129, 161, 434
ScrFI CCNGG 6 cut(s) 378, 650, 704, 713, 826, 919
SdaI CCTGCAGG 1 cut(s) 693
SduI GDGCHC 3 cut(s) 113, 171, 180
SexAI ACCWGGT 1 cut(s) 702
SfcI CTRYAG 2 cut(s) 590, 689
Sfr274I CTCGAG 2 cut(s) 179, 323
Sfr303I CCGCGG 1 cut(s) 59
SgrBI CCGCGG 1 cut(s) 59
SinI GGWCC 3 cut(s) 253, 316, 391
SlaI CTCGAG 2 cut(s) 179, 323
SmiMI CAYNNNNRTG 1 cut(s) 722
SmlI CTYRAG 4 cut(s) 170, 179, 323, 434
SmoI CTYRAG 4 cut(s) 170, 179, 323, 434
Sse8387I CCTGCAGG 1 cut(s) 693
Sse9I AATT 1 cut(s) 922
SspMI CTAG 1 cut(s) 332
SstI GAGCTC 2 cut(s) 171, 180
StyD4I CCNGG 6 cut(s) 376, 648, 702, 711, 824, 917
StyI CCWWGG 3 cut(s) 303, 444, 819
TaaI ACNGT 3 cut(s) 499, 520, 800
TaiI ACGT 5 cut(s) 75, 150, 282, 587, 774
TaqI TCGA 8 cut(s) 78, 89, 129, 150, 180, 324, 753, 762
TaqII GACCGA 1 cut(s) 351
TasI AATT 1 cut(s) 922
TatI WGTACW 1 cut(s) 189
TauI GCSGC 7 cut(s) 58, 61, 98, 101, 350, 469, 980
TfiI GAWTC 1 cut(s) 637
TscAI CASTG 1 cut(s) 805
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 3 cut(s) 697, 811, 877
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 145, 397
TspRI CASTG 1 cut(s) 805
VpaK11BI GGWCC 3 cut(s) 253, 316, 391
XhoI CTCGAG 2 cut(s) 179, 323
XmiI GTMKAC 1 cut(s) 89
XmnI GAANNNNTTC 1 cut(s) 585
XspI CTAG 1 cut(s) 332
ZraI GACGTC 2 cut(s) 148, 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.