Belongs to the syntaxin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 3 cut(s) | 71, 97, 272 |
| AatII | GACGTC | 2 cut(s) | 144, 276 |
| Acc36I | ACCTGC | 1 cut(s) | 687 |
| AccB1I | GGYRCC | 1 cut(s) | 104 |
| AccI | GTMKAC | 2 cut(s) | 89, 183 |
| AccII | CGCG | 5 cut(s) | 31, 58, 63, 180, 486 |
| AccIII | TCCGGA | 2 cut(s) | 36, 417 |
| AclI | AACGTT | 1 cut(s) | 575 |
| AclWI | GGATC | 5 cut(s) | 249, 404, 622, 631, 886 |
| AcuI | CTGAAG | 1 cut(s) | 172 |
| AcyI | GRCGYC | 4 cut(s) | 105, 141, 273, 654 |
| AdeI | CACNNNGTG | 1 cut(s) | 697 |
| AfaI | GTAC | 3 cut(s) | 482, 495, 506 |
| AgeI | ACCGGT | 1 cut(s) | 330 |
| AgsI | TTSAA | 2 cut(s) | 451, 904 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 145 |
| AjiI | CACGTC | 2 cut(s) | 73, 763 |
| AjnI | CCWGG | 6 cut(s) | 315, 630, 639, 693, 702, 815 |
| AluBI | AGCT | 7 cut(s) | 163, 172, 427, 447, 688, 805, 900 |
| AluI | AGCT | 7 cut(s) | 163, 172, 427, 447, 688, 805, 900 |
| Alw21I | GWGCWC | 3 cut(s) | 165, 174, 306 |
| Alw26I | GTCTC | 2 cut(s) | 482, 520 |
| AlwI | GGATC | 5 cut(s) | 249, 404, 622, 631, 886 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 688 |
| Ama87I | CYCGRG | 1 cut(s) | 173 |
| Aor13HI | TCCGGA | 2 cut(s) | 36, 417 |
| AoxI | GGCC | 6 cut(s) | 294, 368, 412, 610, 813, 819 |
| ApeKI | GCWGC | 4 cut(s) | 688, 799, 802, 868 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 116, 148, 747, 779 |
| AsiGI | ACCGGT | 1 cut(s) | 330 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 576 |
| AspLEI | GCGC | 5 cut(s) | 63, 107, 180, 325, 730 |
| AspS9I | GGNCC | 7 cut(s) | 247, 310, 333, 368, 385, 610, 819 |
| AsuC2I | CCSGG | 1 cut(s) | 372 |
| AsuHPI | GGTGA | 2 cut(s) | 268, 799 |
| AvaI | CYCGRG | 1 cut(s) | 173 |
| AvaII | GGWCC | 4 cut(s) | 247, 310, 333, 385 |
| BanI | GGYRCC | 1 cut(s) | 104 |
| BanII | GRGCYC | 2 cut(s) | 165, 174 |
| BauI | CACGAG | 2 cut(s) | 206, 872 |
| BbsI | GAAGAC | 1 cut(s) | 545 |
| Bbv12I | GWGCWC | 3 cut(s) | 165, 174, 306 |
| BbvCI | CCTCAGC | 1 cut(s) | 896 |
| BbvI | GCAGC | 4 cut(s) | 675, 811, 814, 855 |
| BccI | CCATC | 1 cut(s) | 76 |
| BceAI | ACGGC | 5 cut(s) | 108, 224, 427, 641, 892 |
| BciT130I | CCWGG | 6 cut(s) | 317, 632, 641, 695, 704, 817 |
| BcnI | CCSGG | 1 cut(s) | 372 |
| BcoDI | GTCTC | 2 cut(s) | 482, 520 |
| BfmI | CTRYAG | 2 cut(s) | 581, 680 |
| BfoI | RGCGCY | 2 cut(s) | 108, 326 |
| BfuAI | ACCTGC | 1 cut(s) | 687 |
| BglII | AGATCT | 1 cut(s) | 468 |
| Bme1390I | CCNGG | 7 cut(s) | 317, 372, 632, 641, 695, 704, 817 |
| Bme18I | GGWCC | 4 cut(s) | 247, 310, 333, 385 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 145 |
| BmeT110I | CYCGRG | 1 cut(s) | 173 |
| BmgBI | CACGTC | 2 cut(s) | 73, 763 |
| BmgT120I | GGNCC | 7 cut(s) | 247, 310, 333, 368, 385, 610, 819 |
| BmiI | GGNNCC | 6 cut(s) | 22, 35, 106, 353, 611, 677 |
| BmrFI | CCNGG | 7 cut(s) | 317, 372, 632, 641, 695, 704, 817 |
| BpiI | GAAGAC | 1 cut(s) | 545 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 105, 137 |
| BpmI | CTGGAG | 2 cut(s) | 179, 338 |
| Bpu10I | CCTNAGC | 1 cut(s) | 896 |
| BpuEI | CTTGAG | 4 cut(s) | 149, 272, 290, 413 |
| BpuMI | CCSGG | 1 cut(s) | 372 |
| BsaBI | GATNNNNATC | 1 cut(s) | 890 |
| BsaHI | GRCGYC | 4 cut(s) | 105, 141, 273, 654 |
| BsaI | GGTCTC | 1 cut(s) | 482 |
| BsaJI | CCNNGG | 8 cut(s) | 56, 297, 371, 381, 438, 810, 816, 908 |
| BsaWI | WCCGGW | 5 cut(s) | 36, 330, 348, 387, 417 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 500, 530 |
| Bse118I | RCCGGY | 1 cut(s) | 330 |
| Bse1I | ACTGG | 1 cut(s) | 196 |
| Bse3DI | GCAATG | 1 cut(s) | 928 |
| Bse8I | GATNNNNATC | 1 cut(s) | 890 |
| BseAI | TCCGGA | 2 cut(s) | 36, 417 |
| BseBI | CCWGG | 6 cut(s) | 317, 632, 641, 695, 704, 817 |
| BseDI | CCNNGG | 8 cut(s) | 56, 297, 371, 381, 438, 810, 816, 908 |
| BseJI | GATNNNNATC | 1 cut(s) | 890 |
| BseMI | GCAATG | 1 cut(s) | 928 |
| BseMII | CTCAG | 2 cut(s) | 472, 910 |
| BseNI | ACTGG | 1 cut(s) | 196 |
| BseRI | GAGGAG | 2 cut(s) | 59, 542 |
| BseXI | GCAGC | 4 cut(s) | 675, 811, 814, 855 |
| BsgI | GTGCAG | 2 cut(s) | 674, 743 |
| Bsh1236I | CGCG | 5 cut(s) | 31, 58, 63, 180, 486 |
| Bsh1285I | CGRYCG | 1 cut(s) | 331 |
| BshFI | GGCC | 6 cut(s) | 296, 370, 414, 612, 815, 821 |
| BshNI | GGYRCC | 1 cut(s) | 104 |
| BshTI | ACCGGT | 1 cut(s) | 330 |
| BsiEI | CGRYCG | 1 cut(s) | 331 |
| BsiHKAI | GWGCWC | 3 cut(s) | 165, 174, 306 |
| BsiHKCI | CYCGRG | 1 cut(s) | 173 |
| BsiSI | CCGG | 9 cut(s) | 18, 37, 111, 331, 349, 371, 388, 418, 519 |
| BsmAI | GTCTC | 2 cut(s) | 482, 520 |
| BsmBI | CGTCTC | 1 cut(s) | 520 |
| BsnI | GGCC | 6 cut(s) | 296, 370, 414, 612, 815, 821 |
| Bso31I | GGTCTC | 1 cut(s) | 482 |
| BsoBI | CYCGRG | 1 cut(s) | 173 |
| Bsp1286I | GDGCHC | 3 cut(s) | 165, 174, 306 |
| Bsp13I | TCCGGA | 2 cut(s) | 36, 417 |
| Bsp143I | GATC | 9 cut(s) | 67, 254, 396, 468, 627, 636, 741, 885, 891 |
| Bsp19I | CCATGG | 1 cut(s) | 438 |
| BspANI | GGCC | 6 cut(s) | 296, 370, 414, 612, 815, 821 |
| BspCNI | CTCAG | 2 cut(s) | 471, 909 |
| BspEI | TCCGGA | 2 cut(s) | 36, 417 |
| BspFNI | CGCG | 5 cut(s) | 31, 58, 63, 180, 486 |
| BspHI | TCATGA | 1 cut(s) | 765 |
| BspLI | GGNNCC | 6 cut(s) | 22, 35, 106, 353, 611, 677 |
| BspMAI | CTGCAG | 2 cut(s) | 585, 684 |
| BspMI | ACCTGC | 1 cut(s) | 687 |
| BspPI | GGATC | 5 cut(s) | 249, 404, 622, 631, 886 |
| BspQI | GCTCTTC | 1 cut(s) | 206 |
| BspT107I | GGYRCC | 1 cut(s) | 104 |
| BspTNI | GGTCTC | 1 cut(s) | 482 |
| BsrDI | GCAATG | 1 cut(s) | 928 |
| BsrFI | RCCGGY | 1 cut(s) | 330 |
| BsrI | ACTGG | 1 cut(s) | 196 |
| BssAI | RCCGGY | 1 cut(s) | 330 |
| BssECI | CCNNGG | 8 cut(s) | 56, 297, 371, 381, 438, 810, 816, 908 |
| BssMI | GATC | 9 cut(s) | 67, 254, 396, 468, 627, 636, 741, 885, 891 |
| BssNI | GRCGYC | 4 cut(s) | 105, 141, 273, 654 |
| BssSI | CACGAG | 2 cut(s) | 206, 872 |
| BssT1I | CCWWGG | 3 cut(s) | 297, 438, 810 |
| Bst2BI | CACGAG | 2 cut(s) | 206, 872 |
| Bst2UI | CCWGG | 6 cut(s) | 317, 632, 641, 695, 704, 817 |
| Bst4CI | ACNGT | 2 cut(s) | 493, 514 |
| Bst6I | CTCTTC | 1 cut(s) | 206 |
| BstACI | GRCGYC | 4 cut(s) | 105, 141, 273, 654 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 688 |
| BstC8I | GCNNGC | 5 cut(s) | 356, 368, 726, 797, 972 |
| BstDEI | CTNAG | 3 cut(s) | 458, 544, 896 |
| BstDSI | CCRYGG | 3 cut(s) | 56, 438, 908 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 290 |
| BstFNI | CGCG | 5 cut(s) | 31, 58, 63, 180, 486 |
| BstH2I | RGCGCY | 2 cut(s) | 108, 326 |
| BstHHI | GCGC | 5 cut(s) | 63, 107, 180, 325, 730 |
| BstKTI | GATC | 9 cut(s) | 70, 257, 399, 471, 630, 639, 744, 888, 894 |
| BstMAI | GTCTC | 2 cut(s) | 482, 520 |
| BstMBI | GATC | 9 cut(s) | 67, 254, 396, 468, 627, 636, 741, 885, 891 |
| BstMCI | CGRYCG | 1 cut(s) | 331 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 55, 169, 688, 736 |
| BstNI | CCWGG | 6 cut(s) | 317, 632, 641, 695, 704, 817 |
| BstSCI | CCNGG | 7 cut(s) | 315, 370, 630, 639, 693, 702, 815 |
| BstSFI | CTRYAG | 2 cut(s) | 581, 680 |
| BstUI | CGCG | 5 cut(s) | 31, 58, 63, 180, 486 |
| BstV1I | GCAGC | 4 cut(s) | 675, 811, 814, 855 |
| BstV2I | GAAGAC | 1 cut(s) | 545 |
| BstX2I | RGATCY | 3 cut(s) | 396, 468, 636 |
| BstYI | RGATCY | 3 cut(s) | 396, 468, 636 |
| BsuRI | GGCC | 6 cut(s) | 296, 370, 414, 612, 815, 821 |
| BtgI | CCRYGG | 3 cut(s) | 56, 438, 908 |
| BtrI | CACGTC | 2 cut(s) | 73, 763 |
| BveI | ACCTGC | 1 cut(s) | 687 |
| Cac8I | GCNNGC | 5 cut(s) | 356, 368, 726, 797, 972 |
| CaiI | CAGNNNCTG | 1 cut(s) | 688 |
| CciI | TCATGA | 1 cut(s) | 765 |
| CfoI | GCGC | 5 cut(s) | 63, 107, 180, 325, 730 |
| Cfr10I | RCCGGY | 1 cut(s) | 330 |
| Cfr13I | GGNCC | 7 cut(s) | 247, 310, 333, 368, 385, 610, 819 |
| Cfr42I | CCGCGG | 1 cut(s) | 59 |
| CpoI | CGGWCCG | 1 cut(s) | 385 |
| CseI | GACGC | 4 cut(s) | 169, 229, 275, 662 |
| CsiI | ACCWGGT | 1 cut(s) | 693 |
| Csp6I | GTAC | 3 cut(s) | 481, 494, 505 |
| CspAI | ACCGGT | 1 cut(s) | 330 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 910, 945 |
| CspI | CGGWCCG | 1 cut(s) | 385 |
| CviAII | CATG | 5 cut(s) | 262, 439, 667, 709, 766 |
| CviQI | GTAC | 3 cut(s) | 481, 494, 505 |
| DdeI | CTNAG | 3 cut(s) | 458, 544, 896 |
| DinI | GGCGCC | 1 cut(s) | 106 |
| DpnI | GATC | 9 cut(s) | 69, 256, 398, 470, 629, 638, 743, 887, 893 |
| DpnII | GATC | 9 cut(s) | 67, 254, 396, 468, 627, 636, 741, 885, 891 |
| DraIII | CACNNNGTG | 1 cut(s) | 697 |
| DrdI | GACNNNNNNGTC | 3 cut(s) | 71, 97, 272 |
| DriI | GACNNNNNGTC | 1 cut(s) | 145 |
| DseDI | GACNNNNNNGTC | 3 cut(s) | 71, 97, 272 |
| Eam1104I | CTCTTC | 1 cut(s) | 206 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 145 |
| EarI | CTCTTC | 1 cut(s) | 206 |
| EciI | GGCGGA | 2 cut(s) | 302, 951 |
| Ecl136II | GAGCTC | 2 cut(s) | 163, 172 |
| Eco130I | CCWWGG | 3 cut(s) | 297, 438, 810 |
| Eco24I | GRGCYC | 2 cut(s) | 165, 174 |
| Eco31I | GGTCTC | 1 cut(s) | 482 |
| Eco47I | GGWCC | 4 cut(s) | 247, 310, 333, 385 |
| Eco53kI | GAGCTC | 2 cut(s) | 163, 172 |
| Eco57I | CTGAAG | 1 cut(s) | 172 |
| Eco88I | CYCGRG | 1 cut(s) | 173 |
| EcoICRI | GAGCTC | 2 cut(s) | 163, 172 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 290 |
| EcoO109I | RGGNCCY | 2 cut(s) | 247, 819 |
| EcoRII | CCWGG | 6 cut(s) | 315, 630, 639, 693, 702, 815 |
| EcoT14I | CCWWGG | 3 cut(s) | 297, 438, 810 |
| EcoT38I | GRGCYC | 2 cut(s) | 165, 174 |
| EgeI | GGCGCC | 1 cut(s) | 106 |
| EheI | GGCGCC | 1 cut(s) | 106 |
| ErhI | CCWWGG | 3 cut(s) | 297, 438, 810 |
| Esp3I | CGTCTC | 1 cut(s) | 520 |
| FaeI | CATG | 5 cut(s) | 265, 442, 670, 712, 769 |
| FaiI | YATR | 5 cut(s) | 263, 440, 668, 710, 767 |
| FatI | CATG | 5 cut(s) | 261, 438, 666, 708, 765 |
| FauI | CCCGC | 4 cut(s) | 88, 266, 359, 845 |
| FblI | GTMKAC | 2 cut(s) | 89, 183 |
| FriOI | GRGCYC | 2 cut(s) | 165, 174 |
| GlaI | GCGC | 5 cut(s) | 62, 106, 179, 324, 729 |
| GsuI | CTGGAG | 2 cut(s) | 179, 338 |
| HaeII | RGCGCY | 2 cut(s) | 108, 326 |
| HaeIII | GGCC | 6 cut(s) | 296, 370, 414, 612, 815, 821 |
| HapII | CCGG | 9 cut(s) | 18, 37, 111, 331, 349, 371, 388, 418, 519 |
| HgaI | GACGC | 4 cut(s) | 169, 229, 275, 662 |
| HhaI | GCGC | 5 cut(s) | 63, 107, 180, 325, 730 |
| Hin1I | GRCGYC | 4 cut(s) | 105, 141, 273, 654 |
| Hin1II | CATG | 5 cut(s) | 265, 442, 670, 712, 769 |
| Hin6I | GCGC | 5 cut(s) | 61, 105, 178, 323, 728 |
| HinP1I | GCGC | 5 cut(s) | 61, 105, 178, 323, 728 |
| HincII | GTYRAC | 3 cut(s) | 90, 118, 409 |
| HindII | GTYRAC | 3 cut(s) | 90, 118, 409 |
| HinfI | GANTC | 4 cut(s) | 114, 146, 391, 434 |
| HpaII | CCGG | 9 cut(s) | 18, 37, 111, 331, 349, 371, 388, 418, 519 |
| HphI | GGTGA | 2 cut(s) | 268, 799 |
| Hpy166II | GTNNAC | 5 cut(s) | 90, 118, 184, 409, 846 |
| Hpy188I | TCNGA | 5 cut(s) | 254, 385, 396, 478, 885 |
| Hpy188III | TCNNGA | 8 cut(s) | 37, 79, 289, 418, 530, 766, 874, 889 |
| Hpy8I | GTNNAC | 5 cut(s) | 90, 118, 184, 409, 846 |
| Hpy99I | CGWCG | 8 cut(s) | 68, 94, 143, 146, 407, 536, 656, 749 |
| HpyAV | CCTTC | 3 cut(s) | 34, 286, 580 |
| HpyCH4III | ACNGT | 2 cut(s) | 493, 514 |
| HpyCH4IV | ACGT | 5 cut(s) | 72, 141, 273, 575, 762 |
| HpyCH4V | TGCA | 4 cut(s) | 583, 682, 691, 724 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 55, 169, 688, 736 |
| HpyF3I | CTNAG | 3 cut(s) | 458, 544, 896 |
| HpySE526I | ACGT | 5 cut(s) | 72, 141, 273, 575, 762 |
| Hsp92I | GRCGYC | 4 cut(s) | 105, 141, 273, 654 |
| Hsp92II | CATG | 5 cut(s) | 265, 442, 670, 712, 769 |
| HspAI | GCGC | 5 cut(s) | 61, 105, 178, 323, 728 |
| KasI | GGCGCC | 1 cut(s) | 104 |
| Kpn2I | TCCGGA | 2 cut(s) | 36, 417 |
| KspI | CCGCGG | 1 cut(s) | 59 |
| Kzo9I | GATC | 9 cut(s) | 67, 254, 396, 468, 627, 636, 741, 885, 891 |
| LguI | GCTCTTC | 1 cut(s) | 206 |
| Lsp1109I | GCAGC | 4 cut(s) | 675, 811, 814, 855 |
| MabI | ACCWGGT | 1 cut(s) | 693 |
| MaeII | ACGT | 5 cut(s) | 72, 141, 273, 575, 762 |
| MaeIII | GTNAC | 1 cut(s) | 274 |
| MalI | GATC | 9 cut(s) | 69, 256, 398, 470, 629, 638, 743, 887, 893 |
| MboI | GATC | 9 cut(s) | 67, 254, 396, 468, 627, 636, 741, 885, 891 |
| MboII | GAAGA | 6 cut(s) | 124, 223, 433, 478, 550, 841 |
| MflI | RGATCY | 3 cut(s) | 396, 468, 636 |
| MhlI | GDGCHC | 3 cut(s) | 165, 174, 306 |
| MluCI | AATT | 1 cut(s) | 913 |
| Mly113I | GGCGCC | 1 cut(s) | 105 |
| MlyI | GAGTC | 4 cut(s) | 123, 155, 385, 428 |
| MmeI | TCCRAC | 1 cut(s) | 360 |
| MroI | TCCGGA | 2 cut(s) | 36, 417 |
| MroXI | GAANNNNTTC | 1 cut(s) | 576 |
| MslI | CAYNNNNRTG | 2 cut(s) | 80, 713 |
| MspA1I | CMGCKG | 2 cut(s) | 58, 499 |
| MspI | CCGG | 9 cut(s) | 18, 37, 111, 331, 349, 371, 388, 418, 519 |
| MspR9I | CCNGG | 7 cut(s) | 317, 372, 632, 641, 695, 704, 817 |
| MvaI | CCWGG | 6 cut(s) | 317, 632, 641, 695, 704, 817 |
| MvnI | CGCG | 5 cut(s) | 31, 58, 63, 180, 486 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 55, 169, 688, 736 |
| NarI | GGCGCC | 1 cut(s) | 105 |
| NciI | CCSGG | 1 cut(s) | 372 |
| NcoI | CCATGG | 1 cut(s) | 438 |
| NdeII | GATC | 9 cut(s) | 67, 254, 396, 468, 627, 636, 741, 885, 891 |
| NlaIII | CATG | 5 cut(s) | 265, 442, 670, 712, 769 |
| NlaIV | GGNNCC | 6 cut(s) | 22, 35, 106, 353, 611, 677 |
| NmuCI | GTSAC | 1 cut(s) | 274 |
| PaeR7I | CTCGAG | 1 cut(s) | 173 |
| PagI | TCATGA | 1 cut(s) | 765 |
| PciSI | GCTCTTC | 1 cut(s) | 206 |
| PcsI | WCGNNNNNNNCGW | 5 cut(s) | 141, 147, 237, 750, 759 |
| PdmI | GAANNNNTTC | 1 cut(s) | 576 |
| PfoI | TCCNGGA | 3 cut(s) | 630, 639, 702 |
| PinAI | ACCGGT | 1 cut(s) | 330 |
| PleI | GAGTC | 4 cut(s) | 122, 154, 385, 428 |
| PluTI | GGCGCC | 1 cut(s) | 108 |
| PpsI | GAGTC | 4 cut(s) | 122, 154, 385, 428 |
| PpuMI | RGGWCCY | 1 cut(s) | 247 |
| Psp124BI | GAGCTC | 2 cut(s) | 165, 174 |
| Psp1406I | AACGTT | 1 cut(s) | 575 |
| Psp5II | RGGWCCY | 1 cut(s) | 247 |
| Psp6I | CCWGG | 6 cut(s) | 315, 630, 639, 693, 702, 815 |
| PspGI | CCWGG | 6 cut(s) | 315, 630, 639, 693, 702, 815 |
| PspN4I | GGNNCC | 6 cut(s) | 22, 35, 106, 353, 611, 677 |
| PspPI | GGNCC | 7 cut(s) | 247, 310, 333, 368, 385, 610, 819 |
| PspPPI | RGGWCCY | 1 cut(s) | 247 |
| PspXI | VCTCGAGB | 1 cut(s) | 173 |
| PstI | CTGCAG | 2 cut(s) | 585, 684 |
| PstNI | CAGNNNCTG | 1 cut(s) | 688 |
| PsuI | RGATCY | 3 cut(s) | 396, 468, 636 |
| RsaI | GTAC | 3 cut(s) | 482, 495, 506 |
| RsaNI | GTAC | 3 cut(s) | 481, 494, 505 |
| RseI | CAYNNNNRTG | 2 cut(s) | 80, 713 |
| Rsr2I | CGGWCCG | 1 cut(s) | 385 |
| RsrII | CGGWCCG | 1 cut(s) | 385 |
| SacI | GAGCTC | 2 cut(s) | 165, 174 |
| SacII | CCGCGG | 1 cut(s) | 59 |
| SalI | GTCGAC | 1 cut(s) | 88 |
| SapI | GCTCTTC | 1 cut(s) | 206 |
| Sau3AI | GATC | 9 cut(s) | 67, 254, 396, 468, 627, 636, 741, 885, 891 |
| Sau96I | GGNCC | 7 cut(s) | 247, 310, 333, 368, 385, 610, 819 |
| SbfI | CCTGCAGG | 1 cut(s) | 684 |
| SchI | GAGTC | 4 cut(s) | 123, 155, 385, 428 |
| ScrFI | CCNGG | 7 cut(s) | 317, 372, 632, 641, 695, 704, 817 |
| SdaI | CCTGCAGG | 1 cut(s) | 684 |
| SduI | GDGCHC | 3 cut(s) | 165, 174, 306 |
| SexAI | ACCWGGT | 1 cut(s) | 693 |
| SfcI | CTRYAG | 2 cut(s) | 581, 680 |
| SfoI | GGCGCC | 1 cut(s) | 106 |
| Sfr274I | CTCGAG | 1 cut(s) | 173 |
| Sfr303I | CCGCGG | 1 cut(s) | 59 |
| SgrBI | CCGCGG | 1 cut(s) | 59 |
| SinI | GGWCC | 4 cut(s) | 247, 310, 333, 385 |
| SlaI | CTCGAG | 1 cut(s) | 173 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 80, 713 |
| SmlI | CTYRAG | 5 cut(s) | 164, 173, 287, 305, 428 |
| SmoI | CTYRAG | 5 cut(s) | 164, 173, 287, 305, 428 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 684 |
| Sse9I | AATT | 1 cut(s) | 913 |
| SspDI | GGCGCC | 1 cut(s) | 104 |
| SstI | GAGCTC | 2 cut(s) | 165, 174 |
| StyD4I | CCNGG | 7 cut(s) | 315, 370, 630, 639, 693, 702, 815 |
| StyI | CCWWGG | 3 cut(s) | 297, 438, 810 |
| TaaI | ACNGT | 2 cut(s) | 493, 514 |
| TaiI | ACGT | 5 cut(s) | 75, 144, 276, 578, 765 |
| TaqI | TCGA | 9 cut(s) | 78, 89, 123, 144, 174, 327, 621, 744, 753 |
| TasI | AATT | 1 cut(s) | 913 |
| TauI | GCSGC | 6 cut(s) | 58, 61, 110, 344, 967, 970 |
| TseFI | GTSAC | 1 cut(s) | 274 |
| TseI | GCWGC | 4 cut(s) | 688, 799, 802, 868 |
| Tsp45I | GTSAC | 1 cut(s) | 274 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| TspGWI | ACGGA | 2 cut(s) | 139, 391 |
| VpaK11BI | GGWCC | 4 cut(s) | 247, 310, 333, 385 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 290 |
| XhoI | CTCGAG | 1 cut(s) | 173 |
| XmiI | GTMKAC | 2 cut(s) | 89, 183 |
| XmnI | GAANNNNTTC | 1 cut(s) | 576 |
| ZraI | GACGTC | 2 cut(s) | 142, 274 |