MD12G1041800.v1.1

Belongs to the syntaxin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001517
Physical Location & Seq
Forward (+)
4637528 .. 4639583
2056 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1041800.v1.1.491

Sequence Viewer

Length: 879 bp
ATGAACGATTTGCTCTCCAGCTCCTTCTCCGAGGACTCGCCGGACCACCATGTCATTGAAATGGCGGCGACATCCACCGAACTCAACAAGTTTTTCCAGGACGTGGAGTCGGTCCAGAACGAGCTCAATGAGCTTCAAAAGATCAATAAGAGCTTGCAAAGCGCACACGAGCAGAGCAAGAGTCTCCACAACTCCAAGGCAGTCAAGGACCTCCGATCCCGCATGGACGCGGACGTCCATACCGCCCTTAAGACCGCTAAGGTTCTTAAGGTGCAGTTGGATGCATTGGACAAGTCCAACGCCGCCAATCGGAGCTTGCCTGGCTGCGGTCCTGGCTCCTCATCTGACCGGACACGGACGTCGGTGGTCAACGGGCTGAGGAAGAAGCTAAAGGATTCCATGGATAGCTTTAACGACTTGAGGAACAAGATCTCATCGGAGCATAGAGAGACCGTTCAGCGTAGGTACTTCACGGTCACCGGAGATAACCCCGACGACAAAACCCTAGACCTTCTCATCTCTACAGGTGAGAGCGAGACATTTTTACAGAAGGCAATCCAAGAGCAAGGAAGATGGAGGGTTTTGGACACCATACAAGAGATTCAAGAGAGACATGATGGTGTGAAGGCCATGGAAAGGAATCTGAATGAGTTGCACCAGGTGTTCATGGACATGGCAGTTCTTGTGCAAGCTCAAGGTGACCAGTTGGACGACATTCAGGGTCATGTGGAGAGAGCTAACTCGTACGTGCATGCTGGCACCCAGCAGTTGCAGAAGGCCAGGTTCTTCCAGAAAAACAGTCGTAAATGGACTTGTTATGGCATCATACTCTTGCTCATCATCGTTGGGATTATATTGGCCGCGACGCTGCCTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000149 GO:0001101 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0006605 GO:0006612 GO:0006810 GO:0006833 GO:0006886 GO:0006887 GO:0006906 GO:0006950 GO:0006952 GO:0006996 GO:0008104 GO:0008150 GO:0009504 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010119 GO:0010148 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016043 GO:0016050 GO:0016192 GO:0022406 GO:0030054 GO:0031201 GO:0031224 GO:0031347 GO:0031348 GO:0031984 GO:0032507 GO:0032940 GO:0032991 GO:0033036 GO:0033993 GO:0034613 GO:0042044 GO:0042221 GO:0042886 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043495 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045185 GO:0046903 GO:0046907 GO:0048278 GO:0048284 GO:0048519 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051235 GO:0051640 GO:0051641 GO:0051649 GO:0051651 GO:0051704 GO:0051707 GO:0055044 GO:0061024 GO:0061025 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0072657 GO:0072658 GO:0072659 GO:0072660 GO:0080134 GO:0090150 GO:0090174 GO:0097305 GO:0098542 GO:0098791 GO:0098796 GO:0140056 GO:1901700 GO:1990778
KEGG Pathways
Metabolic & Signaling
ko04130 ko04721 map04130 map04721
Pfam Domains
Protein Families
SNARE Syntaxin

Protein Analysis

293

Amino Acids

32.93

Weight (kDa)

6.56

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syntaxin PF00804 26 - 186 8.4e-51 Syntaxin
SNARE PF05739 235 - 286 5.2e-15 SNARE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 50, 233, 358
AatII GACGTC 2 cut(s) 237, 362
AccB1I GGYRCC 1 cut(s) 758
AccB7I CCANNNNNTGG 1 cut(s) 103
AccII CGCG 2 cut(s) 230, 863
AciI CCGC 8 cut(s) 65, 220, 230, 243, 255, 303, 327, 861
AclWI GGATC 1 cut(s) 210
AcoI YGGCCR 1 cut(s) 858
AcyI GRCGYC 2 cut(s) 234, 359
AdeI CACNNNGTG 1 cut(s) 661
AfaI GTAC 2 cut(s) 467, 746
AfiI CCNNNNNNNGG 4 cut(s) 103, 309, 326, 636
AflII CTTAAG 2 cut(s) 248, 266
AgsI TTSAA 3 cut(s) 59, 137, 605
AhdI GACNNNNNGTC 1 cut(s) 106
AjiI CACGTC 1 cut(s) 103
AjnI CCWGG 5 cut(s) 96, 319, 331, 657, 779
AluBI AGCT 9 cut(s) 21, 124, 133, 153, 315, 388, 408, 692, 737
AluI AGCT 9 cut(s) 21, 124, 133, 153, 315, 388, 408, 692, 737
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 4 cut(s) 188, 443, 530, 604
AlwI GGATC 1 cut(s) 210
AoxI GGCC 3 cut(s) 627, 777, 858
ApeKI GCWGC 2 cut(s) 324, 868
AspLEI GCGC 1 cut(s) 164
AspS9I GGNCC 4 cut(s) 43, 112, 208, 329
AsuHPI GGTGA 3 cut(s) 469, 539, 710
AvaII GGWCC 4 cut(s) 43, 112, 208, 329
BanI GGYRCC 1 cut(s) 758
BanII GRGCYC 1 cut(s) 126
BauI CACGAG 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 126
BbvCI CCTCAGC 1 cut(s) 377
BbvI GCAGC 2 cut(s) 311, 855
BccI CCATC 2 cut(s) 567, 611
BciT130I CCWGG 5 cut(s) 98, 321, 333, 659, 781
BcoDI GTCTC 4 cut(s) 188, 443, 530, 604
BfaI CTAG 1 cut(s) 506
BfmI CTRYAG 1 cut(s) 522
BfrI CTTAAG 2 cut(s) 248, 266
BglII AGATCT 1 cut(s) 429
BisI GCNGC 5 cut(s) 66, 303, 325, 861, 869
BlsI GCNGC 5 cut(s) 67, 304, 326, 862, 870
Bme1390I CCNGG 5 cut(s) 98, 321, 333, 659, 781
Bme18I GGWCC 4 cut(s) 43, 112, 208, 329
BmeRI GACNNNNNGTC 1 cut(s) 106
BmgBI CACGTC 1 cut(s) 103
BmgT120I GGNCC 4 cut(s) 43, 112, 208, 329
BmiI GGNNCC 2 cut(s) 337, 760
BmrFI CCNGG 5 cut(s) 98, 321, 333, 659, 781
BmsI GCATC 2 cut(s) 271, 831
Bpu10I CCTNAGC 2 cut(s) 258, 377
BpuEI CTTGAG 2 cut(s) 439, 678
BsaAI YACGTR 1 cut(s) 748
BsaHI GRCGYC 2 cut(s) 234, 359
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 4 cut(s) 30, 195, 399, 630
BsaWI WCCGGW 2 cut(s) 348, 479
Bsc4I CCNNNNNNNGG 4 cut(s) 103, 309, 326, 636
Bse1I ACTGG 1 cut(s) 703
BseBI CCWGG 5 cut(s) 98, 321, 333, 659, 781
BseDI CCNNGG 4 cut(s) 30, 195, 399, 630
BseGI GGATG 2 cut(s) 71, 286
BseLI CCNNNNNNNGG 4 cut(s) 103, 309, 326, 636
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 1 cut(s) 703
BseRI GAGGAG 1 cut(s) 328
BseXI GCAGC 2 cut(s) 311, 855
BseYI CCCAGC 1 cut(s) 762
BsgI GTGCAG 1 cut(s) 293
Bsh1236I CGCG 2 cut(s) 230, 863
BshFI GGCC 3 cut(s) 629, 779, 860
BshNI GGYRCC 1 cut(s) 758
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 3 cut(s) 41, 349, 480
BsiWI CGTACG 1 cut(s) 744
BslI CCNNNNNNNGG 4 cut(s) 103, 309, 326, 636
BsmAI GTCTC 4 cut(s) 188, 443, 530, 604
BsnI GGCC 3 cut(s) 629, 779, 860
Bso31I GGTCTC 1 cut(s) 443
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 3 cut(s) 141, 215, 429
Bsp19I CCATGG 2 cut(s) 399, 630
BspACI CCGC 8 cut(s) 65, 220, 230, 243, 255, 303, 327, 861
BspANI GGCC 3 cut(s) 629, 779, 860
BspCNI CTCAG 1 cut(s) 369
BspFNI CGCG 2 cut(s) 230, 863
BspLI GGNNCC 2 cut(s) 337, 760
BspPI GGATC 1 cut(s) 210
BspT107I GGYRCC 1 cut(s) 758
BspTI CTTAAG 2 cut(s) 248, 266
BspTNI GGTCTC 1 cut(s) 443
BsrI ACTGG 1 cut(s) 703
BssECI CCNNGG 4 cut(s) 30, 195, 399, 630
BssMI GATC 3 cut(s) 141, 215, 429
BssNI GRCGYC 2 cut(s) 234, 359
BssSI CACGAG 1 cut(s) 167
BssT1I CCWWGG 3 cut(s) 195, 399, 630
Bst2BI CACGAG 1 cut(s) 167
Bst2UI CCWGG 5 cut(s) 98, 321, 333, 659, 781
Bst4CI ACNGT 3 cut(s) 454, 475, 800
BstACI GRCGYC 2 cut(s) 234, 359
BstAFI CTTAAG 2 cut(s) 248, 266
BstBAI YACGTR 1 cut(s) 748
BstC8I GCNNGC 5 cut(s) 155, 317, 690, 753, 757
BstDEI CTNAG 2 cut(s) 258, 377
BstDSI CCRYGG 2 cut(s) 399, 630
BstEII GGTNACC 2 cut(s) 475, 698
BstF5I GGATG 2 cut(s) 71, 286
BstFNI CGCG 2 cut(s) 230, 863
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 3 cut(s) 144, 218, 432
BstMAI GTCTC 4 cut(s) 188, 443, 530, 604
BstMBI GATC 3 cut(s) 141, 215, 429
BstMWI GCNNNNNNNGC 4 cut(s) 130, 159, 321, 333
BstNI CCWGG 5 cut(s) 98, 321, 333, 659, 781
BstNSI RCATGY 1 cut(s) 755
BstPI GGTNACC 2 cut(s) 475, 698
BstSCI CCNGG 5 cut(s) 96, 319, 331, 657, 779
BstSFI CTRYAG 1 cut(s) 522
BstUI CGCG 2 cut(s) 230, 863
BstV1I GCAGC 2 cut(s) 311, 855
BstX2I RGATCY 1 cut(s) 429
BstYI RGATCY 1 cut(s) 429
BsuRI GGCC 3 cut(s) 629, 779, 860
BtgI CCRYGG 2 cut(s) 399, 630
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 2 cut(s) 71, 286
Cac8I GCNNGC 5 cut(s) 155, 317, 690, 753, 757
CfoI GCGC 1 cut(s) 164
Cfr13I GGNCC 4 cut(s) 43, 112, 208, 329
CseI GACGC 2 cut(s) 236, 874
CsiI ACCWGGT 1 cut(s) 657
Csp6I GTAC 2 cut(s) 466, 745
CviAII CATG 9 cut(s) 50, 223, 400, 614, 631, 667, 673, 725, 752
CviQI GTAC 2 cut(s) 466, 745
DdeI CTNAG 2 cut(s) 258, 377
DpnI GATC 3 cut(s) 143, 217, 431
DpnII GATC 3 cut(s) 141, 215, 429
DraIII CACNNNGTG 1 cut(s) 661
DrdI GACNNNNNNGTC 3 cut(s) 50, 233, 358
DriI GACNNNNNGTC 1 cut(s) 106
DseDI GACNNNNNNGTC 3 cut(s) 50, 233, 358
EaeI YGGCCR 1 cut(s) 858
Eam1105I GACNNNNNGTC 1 cut(s) 106
Ecl136II GAGCTC 1 cut(s) 124
Eco130I CCWWGG 3 cut(s) 195, 399, 630
Eco24I GRGCYC 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 443
Eco47I GGWCC 4 cut(s) 43, 112, 208, 329
Eco53kI GAGCTC 1 cut(s) 124
Eco91I GGTNACC 2 cut(s) 475, 698
EcoICRI GAGCTC 1 cut(s) 124
EcoO109I RGGNCCY 1 cut(s) 208
EcoO65I GGTNACC 2 cut(s) 475, 698
EcoRII CCWGG 5 cut(s) 96, 319, 331, 657, 779
EcoT14I CCWWGG 3 cut(s) 195, 399, 630
EcoT22I ATGCAT 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 3 cut(s) 195, 399, 630
FaeI CATG 9 cut(s) 53, 226, 403, 617, 634, 670, 676, 728, 755
FatI CATG 9 cut(s) 49, 222, 399, 613, 630, 666, 672, 724, 751
FauI CCCGC 1 cut(s) 227
Fnu4HI GCNGC 5 cut(s) 66, 303, 325, 861, 869
FokI GGATG 2 cut(s) 58, 293
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 5 cut(s) 66, 303, 325, 861, 869
FspBI CTAG 1 cut(s) 506
GlaI GCGC 1 cut(s) 163
GluI GCNGC 5 cut(s) 66, 303, 325, 861, 869
GsaI CCCAGC 1 cut(s) 766
HaeIII GGCC 3 cut(s) 629, 779, 860
HapII CCGG 3 cut(s) 41, 349, 480
HgaI GACGC 2 cut(s) 236, 874
HhaI GCGC 1 cut(s) 164
Hin1I GRCGYC 2 cut(s) 234, 359
Hin1II CATG 9 cut(s) 53, 226, 403, 617, 634, 670, 676, 728, 755
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 6 cut(s) 35, 107, 181, 395, 601, 640
HpaII CCGG 3 cut(s) 41, 349, 480
HphI GGTGA 3 cut(s) 469, 539, 710
Hpy166II GTNNAC 1 cut(s) 370
Hpy188I TCNGA 6 cut(s) 31, 215, 312, 346, 439, 645
Hpy188III TCNNGA 3 cut(s) 115, 605, 790
Hpy8I GTNNAC 1 cut(s) 370
Hpy99I CGWCG 3 cut(s) 364, 497, 868
HpyAV CCTTC 5 cut(s) 34, 521, 544, 619, 769
HpyCH4III ACNGT 3 cut(s) 454, 475, 800
HpyCH4IV ACGT 4 cut(s) 102, 234, 359, 747
HpyCH4V TGCA 7 cut(s) 157, 274, 284, 655, 688, 751, 772
HpyF10VI GCNNNNNNNGC 4 cut(s) 130, 159, 321, 333
HpyF3I CTNAG 2 cut(s) 258, 377
HpySE526I ACGT 4 cut(s) 102, 234, 359, 747
Hsp92I GRCGYC 2 cut(s) 234, 359
Hsp92II CATG 9 cut(s) 53, 226, 403, 617, 634, 670, 676, 728, 755
HspAI GCGC 1 cut(s) 162
Kzo9I GATC 3 cut(s) 141, 215, 429
LmnI GCTCC 4 cut(s) 26, 312, 341, 439
Lsp1109I GCAGC 2 cut(s) 311, 855
LweI GCATC 2 cut(s) 271, 831
MabI ACCWGGT 1 cut(s) 657
MaeI CTAG 1 cut(s) 506
MaeII ACGT 4 cut(s) 102, 234, 359, 747
MaeIII GTNAC 2 cut(s) 475, 698
MalI GATC 3 cut(s) 143, 217, 431
MboI GATC 3 cut(s) 141, 215, 429
MboII GAAGA 3 cut(s) 394, 582, 778
MflI RGATCY 1 cut(s) 429
MhlI GDGCHC 1 cut(s) 126
MlyI GAGTC 3 cut(s) 29, 116, 190
MmeI TCCRAC 3 cut(s) 258, 321, 687
MnlI CCTC 6 cut(s) 25, 221, 349, 372, 414, 570
Mph1103I ATGCAT 1 cut(s) 286
MseI TTAA 3 cut(s) 249, 267, 411
MslI CAYNNNNRTG 3 cut(s) 59, 618, 671
MspCI CTTAAG 2 cut(s) 248, 266
MspI CCGG 3 cut(s) 41, 349, 480
MspR9I CCNGG 5 cut(s) 98, 321, 333, 659, 781
MvaI CCWGG 5 cut(s) 98, 321, 333, 659, 781
MvnI CGCG 2 cut(s) 230, 863
MwoI GCNNNNNNNGC 4 cut(s) 130, 159, 321, 333
NcoI CCATGG 2 cut(s) 399, 630
NdeII GATC 3 cut(s) 141, 215, 429
NlaIII CATG 9 cut(s) 53, 226, 403, 617, 634, 670, 676, 728, 755
NlaIV GGNNCC 2 cut(s) 337, 760
NmuCI GTSAC 2 cut(s) 475, 698
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 1 cut(s) 755
PaeI GCATGC 1 cut(s) 755
PfeI GAWTC 3 cut(s) 395, 601, 640
Pfl23II CGTACG 1 cut(s) 744
PflMI CCANNNNNTGG 1 cut(s) 103
PfoI TCCNGGA 1 cut(s) 96
PkrI GCNGC 5 cut(s) 67, 304, 326, 862, 870
PleI GAGTC 3 cut(s) 29, 115, 189
PpsI GAGTC 3 cut(s) 29, 115, 189
Ppu21I YACGTR 1 cut(s) 748
PpuMI RGGWCCY 1 cut(s) 208
Psp124BI GAGCTC 1 cut(s) 126
Psp5II RGGWCCY 1 cut(s) 208
Psp6I CCWGG 5 cut(s) 96, 319, 331, 657, 779
PspEI GGTNACC 2 cut(s) 475, 698
PspFI CCCAGC 1 cut(s) 762
PspGI CCWGG 5 cut(s) 96, 319, 331, 657, 779
PspLI CGTACG 1 cut(s) 744
PspN4I GGNNCC 2 cut(s) 337, 760
PspPI GGNCC 4 cut(s) 43, 112, 208, 329
PspPPI RGGWCCY 1 cut(s) 208
PsuI RGATCY 1 cut(s) 429
RsaI GTAC 2 cut(s) 467, 746
RsaNI GTAC 2 cut(s) 466, 745
RseI CAYNNNNRTG 3 cut(s) 59, 618, 671
SacI GAGCTC 1 cut(s) 126
SaqAI TTAA 3 cut(s) 249, 267, 411
SatI GCNGC 5 cut(s) 66, 303, 325, 861, 869
Sau3AI GATC 3 cut(s) 141, 215, 429
Sau96I GGNCC 4 cut(s) 43, 112, 208, 329
SchI GAGTC 3 cut(s) 29, 116, 190
ScrFI CCNGG 5 cut(s) 98, 321, 333, 659, 781
SduI GDGCHC 1 cut(s) 126
SexAI ACCWGGT 1 cut(s) 657
SfaNI GCATC 2 cut(s) 271, 831
SfcI CTRYAG 1 cut(s) 522
SinI GGWCC 4 cut(s) 43, 112, 208, 329
SmiMI CAYNNNNRTG 3 cut(s) 59, 618, 671
SmlI CTYRAG 4 cut(s) 248, 266, 418, 693
SmoI CTYRAG 4 cut(s) 248, 266, 418, 693
SphI GCATGC 1 cut(s) 755
SsiI CCGC 8 cut(s) 65, 220, 230, 243, 255, 303, 327, 861
SspMI CTAG 1 cut(s) 506
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 5 cut(s) 96, 319, 331, 657, 779
StyI CCWWGG 3 cut(s) 195, 399, 630
TaaI ACNGT 3 cut(s) 454, 475, 800
TaiI ACGT 4 cut(s) 105, 237, 362, 750
TaqII GACCGA 1 cut(s) 100
TauI GCSGC 3 cut(s) 68, 305, 863
TfiI GAWTC 3 cut(s) 395, 601, 640
Tru1I TTAA 3 cut(s) 249, 267, 411
Tru9I TTAA 3 cut(s) 249, 267, 411
TseFI GTSAC 2 cut(s) 475, 698
TseI GCWGC 2 cut(s) 324, 868
Tsp45I GTSAC 2 cut(s) 475, 698
TspDTI ATGAA 2 cut(s) 17, 655
TspGWI ACGGA 1 cut(s) 370
Van91I CCANNNNNTGG 1 cut(s) 103
Vha464I CTTAAG 2 cut(s) 248, 266
VpaK11BI GGWCC 4 cut(s) 43, 112, 208, 329
XceI RCATGY 1 cut(s) 755
XspI CTAG 1 cut(s) 506
ZraI GACGTC 2 cut(s) 235, 360
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.