FvH4_6g23970

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002314
Physical Location & Seq
Forward (+)
18028027 .. 18032562
4536 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g23970.t1

Sequence Viewer

Length: 1581 bp
ATGGTATACCACCAGCGCAAAAAAGGTGAAAAGCCTAGGGAGGAAGAAGATGATGAGGAGTGTCAGACCCTTGCTATGGAGTGTGCGAGCTCTAGTTATTACAAAAGATCAACTTCAAAGCTTTTCACCTTTCTCTTCATCTGTATTCTCTCTTGTAGCTTCATATTAGCCCCTCATCTGATATTTTCCTCTGTCCCCACTTTCTCCCTTTCAAAACCACCGGAAGGAGAAAATGAAGGCCCGAGTACTGCTGCTGTTACCGACGCAAAGGCTCCCTTGTATTCTTCGATGTCTAATGCTGCAGGAGCTATTTGTTGTGATAGAAGCAGTATGCGTTCAGATGTTTGTACCATGAAAGGGGATGTAAGAACACACTCGCCTTCCTCTTCTATCTTCGTTTACGCATCGCAAGACCAAGCAGTGAAGAATGAGGAGGACCTCCAACACGAAAAGATCAAACCCTACACCAGAAAATGGGAAACAATTATTATGGACACCATTGTTGAGTTGGACCTCATTGCAAAGAAACGTACTCTGATCATGAACCATCATAACTGTGATGTCCAACATGATGTTCCGGCAGTGTTTTTCTCAACCGGGGGTTACACCGGCAACGTCTACCATGAATTTAATGATGGGATTATGCCACTGTACATTACTTCCCAACATTTCAACAAGAAGGTTGTGTTCGTCATTCTTGAATTTCACAATTGGTGGGTCATGAAATATTCGGACATTCTCTCCCATCTCTCAGATTATCCTGTAATAGATTTCCATACAGATGAGAGAGTTCATTGCTTCCCTGAGGCCATCGTTGGTTTGAAAATTCACGATGAGCTCACTGTGGATTCTTCATTGATGGAAGGGAATAAGAGCATCGTCGATTTCCGAGAACTCCTTGACAGAGCTTACCAGCCTCAGATTAGAAGTCTAATGCATATGCATGAACATGCAAAGGTTTCTGATGTTCTTTCGAAATCGAAAAGGCCATCCAGAATCAGGAGAAATGTGCAAGAGCGTGCATTCAATAACGAGCCGAAGTTGGTTATCATATCTCGGAATGGATCAAGGGCGATAACCAATGAGAATTTGCTGGTGAAAATGGCTGAGAAAATCGGGTTTCATGTCCAAGTTTTGAGGCCGGATAGCAGGACAATGTTAGCAGAGATTTATTGGGCTCTAAATTCGAGCGATGTCATGATTGGGGTTCATGGTGCTGCCATGACACATTTTCTGTTTATGAAGCCTGGTTCTGTGTTTATCCAAGTCATTCCTCTCGGAATTGAATGGGCAGCAGAGGCATACTATGGAGAACCTGCAAAGAAGCTTGGGTTAAACTACATTGGATACCAAATCCTTACTAAAGAGAGCTCGTTATGTGACAAATATAACAAGGATGATCCTGTTCTTAAAGATCCAAGGAGTGTAACCAGAAAGGGATGGCAATACACAAAGATGATCTATCTTGAGGGTCAGACGGTGAGACTAGACCTCCCAAGATTTCGGAAACAATTGGTTGGCGCTTACAATTACACCATTGATAGATTGAAACAGCAGCGTTCCAATATTCAGTCACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

527

Amino Acids

60.28

Weight (kDa)

7.64

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 327 - 421 5.7e-14 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1324
AccB7I CCANNNNNTGG 1 cut(s) 474
AccI GTMKAC 2 cut(s) 6, 618
AclWI GGATC 3 cut(s) 1072, 1394, 1409
AcsI RAATTY 5 cut(s) 626, 701, 825, 1087, 1183
AfaI GTAC 4 cut(s) 247, 349, 532, 653
AfiI CCNNNNNNNGG 5 cut(s) 76, 224, 357, 474, 999
AgsI TTSAA 8 cut(s) 117, 213, 673, 701, 823, 1027, 1286, 1549
AjnI CCWGG 1 cut(s) 1246
AjuI GAANNNNNNNTTGG 2 cut(s) 558, 590
AluBI AGCT 8 cut(s) 90, 121, 159, 308, 838, 908, 1327, 1371
AluI AGCT 8 cut(s) 90, 121, 159, 308, 838, 908, 1327, 1371
Alw21I GWGCWC 3 cut(s) 92, 840, 1373
Alw26I GTCTC 1 cut(s) 1477
AlwI GGATC 3 cut(s) 1072, 1394, 1409
Ama87I CYCGRG 1 cut(s) 241
AoxI GGCC 4 cut(s) 238, 807, 986, 1139
ApeKI GCWGC 5 cut(s) 251, 299, 1217, 1292, 1555
ApoI RAATTY 5 cut(s) 626, 701, 825, 1087, 1183
AspA2I CCTAGG 1 cut(s) 35
AspLEI GCGC 2 cut(s) 18, 1523
AspS9I GGNCC 3 cut(s) 239, 436, 511
AsuC2I CCSGG 1 cut(s) 598
AsuHPI GGTGA 4 cut(s) 38, 118, 1108, 1492
AsuII TTCGAA 1 cut(s) 974
AvaI CYCGRG 1 cut(s) 241
AvaII GGWCC 2 cut(s) 436, 511
AvrII CCTAGG 1 cut(s) 35
AxyI CCTNAGG 1 cut(s) 804
BanII GRGCYC 4 cut(s) 92, 840, 1180, 1373
Bbv12I GWGCWC 3 cut(s) 92, 840, 1373
BbvI GCAGC 5 cut(s) 238, 286, 1204, 1304, 1567
BccI CCATC 7 cut(s) 555, 629, 753, 818, 853, 997, 1434
BciT130I CCWGG 1 cut(s) 1248
BciVI GTATCC 1 cut(s) 1340
BclI TGATCA 1 cut(s) 537
BcnI CCSGG 1 cut(s) 598
BcoDI GTCTC 1 cut(s) 1477
BfaI CTAG 3 cut(s) 36, 93, 1487
BfmI CTRYAG 1 cut(s) 300
BfoI RGCGCY 1 cut(s) 1524
BfuAI ACCTGC 1 cut(s) 1324
BfuI GTATCC 1 cut(s) 1340
BisI GCNGC 5 cut(s) 252, 300, 1218, 1293, 1556
BlnI CCTAGG 1 cut(s) 35
BlsI GCNGC 5 cut(s) 253, 301, 1219, 1294, 1557
BmcAI AGTACT 1 cut(s) 247
Bme1390I CCNGG 2 cut(s) 598, 1248
Bme18I GGWCC 2 cut(s) 436, 511
BmeT110I CYCGRG 1 cut(s) 241
BmgT120I GGNCC 3 cut(s) 239, 436, 511
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 2 cut(s) 598, 1248
BmsI GCATC 2 cut(s) 413, 885
Bpu14I TTCGAA 1 cut(s) 974
BpuEI CTTGAG 1 cut(s) 1487
BpuMI CCSGG 1 cut(s) 598
BsaJI CCNNGG 3 cut(s) 35, 597, 1418
BsaWI WCCGGW 1 cut(s) 220
BsaXI ACNNNNNCTCC 4 cut(s) 725, 755, 1476, 1506
Bsc4I CCNNNNNNNGG 5 cut(s) 76, 224, 357, 474, 999
Bse118I RCCGGY 1 cut(s) 608
Bse21I CCTNAGG 1 cut(s) 804
Bse3DI GCAATG 2 cut(s) 516, 793
BseBI CCWGG 1 cut(s) 1248
BseDI CCNNGG 3 cut(s) 35, 597, 1418
BseGI GGATG 4 cut(s) 367, 989, 1402, 1445
BseLI CCNNNNNNNGG 5 cut(s) 76, 224, 357, 474, 999
BseMI GCAATG 2 cut(s) 516, 793
BseMII CTCAG 4 cut(s) 765, 795, 932, 1098
BseRI GAGGAG 2 cut(s) 71, 446
BseXI GCAGC 5 cut(s) 238, 286, 1204, 1304, 1567
BshFI GGCC 4 cut(s) 240, 809, 988, 1141
BsiHKAI GWGCWC 3 cut(s) 92, 840, 1373
BsiHKCI CYCGRG 1 cut(s) 241
BsiSI CCGG 5 cut(s) 221, 578, 597, 609, 1142
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 5 cut(s) 76, 224, 357, 474, 999
BsmAI GTCTC 1 cut(s) 1477
BsmFI GGGAC 1 cut(s) 179
BsmI GAATGC 1 cut(s) 1022
BsnI GGCC 4 cut(s) 240, 809, 988, 1141
BsoBI CYCGRG 1 cut(s) 241
Bsp119I TTCGAA 1 cut(s) 974
Bsp1286I GDGCHC 4 cut(s) 92, 840, 1180, 1373
Bsp1407I TGTACA 1 cut(s) 651
Bsp143I GATC 7 cut(s) 107, 453, 537, 1064, 1399, 1414, 1458
BspANI GGCC 4 cut(s) 240, 809, 988, 1141
BspCNI CTCAG 4 cut(s) 764, 796, 931, 1099
BspHI TCATGA 3 cut(s) 540, 720, 1197
BspLI GGNNCC 1 cut(s) 273
BspMAI CTGCAG 1 cut(s) 304
BspMI ACCTGC 1 cut(s) 1324
BspPI GGATC 3 cut(s) 1072, 1394, 1409
BspT104I TTCGAA 1 cut(s) 974
BsrDI GCAATG 2 cut(s) 516, 793
BsrFI RCCGGY 1 cut(s) 608
BsrGI TGTACA 1 cut(s) 651
BssAI RCCGGY 1 cut(s) 608
BssECI CCNNGG 3 cut(s) 35, 597, 1418
BssMI GATC 7 cut(s) 107, 453, 537, 1064, 1399, 1414, 1458
BssNAI GTATAC 1 cut(s) 7
BssT1I CCWWGG 2 cut(s) 35, 1418
Bst1107I GTATAC 1 cut(s) 7
Bst2UI CCWGG 1 cut(s) 1248
Bst4CI ACNGT 4 cut(s) 557, 651, 844, 1480
Bst6I CTCTTC 2 cut(s) 140, 391
BstAUI TGTACA 1 cut(s) 651
BstBI TTCGAA 1 cut(s) 974
BstC8I GCNNGC 2 cut(s) 88, 1020
BstDEI CTNAG 4 cut(s) 751, 804, 918, 1107
BstF5I GGATG 4 cut(s) 367, 989, 1402, 1445
BstH2I RGCGCY 1 cut(s) 1524
BstHHI GCGC 2 cut(s) 18, 1523
BstKTI GATC 7 cut(s) 110, 456, 540, 1067, 1402, 1417, 1461
BstMAI GTCTC 1 cut(s) 1477
BstMBI GATC 7 cut(s) 107, 453, 537, 1064, 1399, 1414, 1458
BstMWI GCNNNNNNNGC 2 cut(s) 305, 1298
BstNI CCWGG 1 cut(s) 1248
BstNSI RCATGY 1 cut(s) 953
BstSCI CCNGG 2 cut(s) 596, 1246
BstSFI CTRYAG 1 cut(s) 300
BstV1I GCAGC 5 cut(s) 238, 286, 1204, 1304, 1567
BstX2I RGATCY 1 cut(s) 1414
BstYI RGATCY 1 cut(s) 1414
BstZ17I GTATAC 1 cut(s) 7
Bsu36I CCTNAGG 1 cut(s) 804
BsuI GTATCC 1 cut(s) 1340
BsuRI GGCC 4 cut(s) 240, 809, 988, 1141
BtgZI GCGATG 2 cut(s) 390, 1206
BtsCI GGATG 4 cut(s) 367, 989, 1402, 1445
BtsI GCAGTG 2 cut(s) 426, 588
BtsIMutI CAGTG 4 cut(s) 426, 588, 647, 840
BveI ACCTGC 1 cut(s) 1324
Cac8I GCNNGC 2 cut(s) 88, 1020
CciI TCATGA 3 cut(s) 540, 720, 1197
CfoI GCGC 2 cut(s) 18, 1523
Cfr10I RCCGGY 1 cut(s) 608
Cfr13I GGNCC 3 cut(s) 239, 436, 511
CseI GACGC 1 cut(s) 272
Csp6I GTAC 4 cut(s) 246, 348, 531, 652
CviQI GTAC 4 cut(s) 246, 348, 531, 652
DdeI CTNAG 4 cut(s) 751, 804, 918, 1107
DpnI GATC 7 cut(s) 109, 455, 539, 1066, 1401, 1416, 1460
DpnII GATC 7 cut(s) 107, 453, 537, 1064, 1399, 1414, 1458
Eam1104I CTCTTC 2 cut(s) 140, 391
EarI CTCTTC 2 cut(s) 140, 391
Ecl136II GAGCTC 3 cut(s) 90, 838, 1371
Eco130I CCWWGG 2 cut(s) 35, 1418
Eco24I GRGCYC 4 cut(s) 92, 840, 1180, 1373
Eco47I GGWCC 2 cut(s) 436, 511
Eco53kI GAGCTC 3 cut(s) 90, 838, 1371
Eco81I CCTNAGG 1 cut(s) 804
Eco88I CYCGRG 1 cut(s) 241
EcoICRI GAGCTC 3 cut(s) 90, 838, 1371
EcoO109I RGGNCCY 1 cut(s) 436
EcoRII CCWGG 1 cut(s) 1246
EcoT14I CCWWGG 2 cut(s) 35, 1418
EcoT22I ATGCAT 2 cut(s) 939, 945
EcoT38I GRGCYC 4 cut(s) 92, 840, 1180, 1373
ErhI CCWWGG 2 cut(s) 35, 1418
FalI AAGNNNNNCTT 4 cut(s) 97, 129, 260, 292
FaqI GGGAC 1 cut(s) 179
FauNDI CATATG 1 cut(s) 939
FbaI TGATCA 1 cut(s) 537
FblI GTMKAC 2 cut(s) 6, 618
Fnu4HI GCNGC 5 cut(s) 252, 300, 1218, 1293, 1556
FokI GGATG 4 cut(s) 374, 976, 1409, 1452
FriOI GRGCYC 4 cut(s) 92, 840, 1180, 1373
Fsp4HI GCNGC 5 cut(s) 252, 300, 1218, 1293, 1556
FspBI CTAG 3 cut(s) 36, 93, 1487
GlaI GCGC 2 cut(s) 17, 1522
GluI GCNGC 5 cut(s) 252, 300, 1218, 1293, 1556
HaeII RGCGCY 1 cut(s) 1524
HaeIII GGCC 4 cut(s) 240, 809, 988, 1141
HapII CCGG 5 cut(s) 221, 578, 597, 609, 1142
HgaI GACGC 1 cut(s) 272
HhaI GCGC 2 cut(s) 18, 1523
Hin6I GCGC 2 cut(s) 16, 1521
HinP1I GCGC 2 cut(s) 16, 1521
HindIII AAGCTT 2 cut(s) 119, 1325
HinfI GANTC 2 cut(s) 848, 996
HpaII CCGG 5 cut(s) 221, 578, 597, 609, 1142
HphI GGTGA 4 cut(s) 38, 118, 1108, 1492
Hpy166II GTNNAC 3 cut(s) 7, 400, 619
Hpy188III TCNNGA 8 cut(s) 541, 698, 721, 830, 993, 1000, 1198, 1466
Hpy8I GTNNAC 3 cut(s) 7, 400, 619
Hpy99I CGWCG 2 cut(s) 266, 884
HpyAV CCTTC 5 cut(s) 218, 230, 390, 673, 857
HpyCH4III ACNGT 4 cut(s) 557, 651, 844, 1480
HpyCH4IV ACGT 2 cut(s) 529, 615
HpyCH4V TGCA 8 cut(s) 302, 521, 937, 943, 953, 1012, 1022, 1319
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 1298
HpyF3I CTNAG 4 cut(s) 751, 804, 918, 1107
HpySE526I ACGT 2 cut(s) 529, 615
HspAI GCGC 2 cut(s) 16, 1521
Ksp22I TGATCA 1 cut(s) 537
Kzo9I GATC 7 cut(s) 107, 453, 537, 1064, 1399, 1414, 1458
LmnI GCTCC 2 cut(s) 277, 305
Lsp1109I GCAGC 5 cut(s) 238, 286, 1204, 1304, 1567
LweI GCATC 2 cut(s) 413, 885
MaeI CTAG 3 cut(s) 36, 93, 1487
MaeII ACGT 2 cut(s) 529, 615
MaeIII GTNAC 5 cut(s) 256, 602, 1379, 1426, 1572
MalI GATC 7 cut(s) 109, 455, 539, 1066, 1401, 1416, 1460
MboI GATC 7 cut(s) 107, 453, 537, 1064, 1399, 1414, 1458
MboII GAAGA 8 cut(s) 56, 59, 127, 276, 378, 385, 436, 843
MfeI CAATTG 2 cut(s) 709, 1511
MflI RGATCY 1 cut(s) 1414
MhlI GDGCHC 4 cut(s) 92, 840, 1180, 1373
MmeI TCCRAC 3 cut(s) 466, 489, 589
Mph1103I ATGCAT 2 cut(s) 939, 945
MseI TTAA 3 cut(s) 630, 1334, 1410
MslI CAYNNNNRTG 5 cut(s) 555, 780, 942, 948, 1454
MspI CCGG 5 cut(s) 221, 578, 597, 609, 1142
MspR9I CCNGG 2 cut(s) 598, 1248
MunI CAATTG 2 cut(s) 709, 1511
Mva1269I GAATGC 1 cut(s) 1022
MvaI CCWGG 1 cut(s) 1248
MwoI GCNNNNNNNGC 2 cut(s) 305, 1298
NciI CCSGG 1 cut(s) 598
NdeI CATATG 1 cut(s) 939
NdeII GATC 7 cut(s) 107, 453, 537, 1064, 1399, 1414, 1458
NlaIV GGNNCC 1 cut(s) 273
NmuCI GTSAC 2 cut(s) 1379, 1572
NsiI ATGCAT 2 cut(s) 939, 945
NspI RCATGY 1 cut(s) 953
NspV TTCGAA 1 cut(s) 974
PagI TCATGA 3 cut(s) 540, 720, 1197
PctI GAATGC 1 cut(s) 1022
PfeI GAWTC 2 cut(s) 848, 996
PflMI CCANNNNNTGG 1 cut(s) 474
PkrI GCNGC 5 cut(s) 253, 301, 1219, 1294, 1557
PpuMI RGGWCCY 1 cut(s) 436
Psp124BI GAGCTC 3 cut(s) 92, 840, 1373
Psp5II RGGWCCY 1 cut(s) 436
Psp6I CCWGG 1 cut(s) 1246
PspGI CCWGG 1 cut(s) 1246
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 3 cut(s) 239, 436, 511
PspPPI RGGWCCY 1 cut(s) 436
PstI CTGCAG 1 cut(s) 304
PsuI RGATCY 1 cut(s) 1414
RsaI GTAC 4 cut(s) 247, 349, 532, 653
RsaNI GTAC 4 cut(s) 246, 348, 531, 652
RseI CAYNNNNRTG 5 cut(s) 555, 780, 942, 948, 1454
SacI GAGCTC 3 cut(s) 92, 840, 1373
SaqAI TTAA 3 cut(s) 630, 1334, 1410
SatI GCNGC 5 cut(s) 252, 300, 1218, 1293, 1556
Sau3AI GATC 7 cut(s) 107, 453, 537, 1064, 1399, 1414, 1458
Sau96I GGNCC 3 cut(s) 239, 436, 511
ScaI AGTACT 1 cut(s) 247
ScrFI CCNGG 2 cut(s) 598, 1248
SduI GDGCHC 4 cut(s) 92, 840, 1180, 1373
SfaNI GCATC 2 cut(s) 413, 885
SfcI CTRYAG 1 cut(s) 300
SfuI TTCGAA 1 cut(s) 974
SinI GGWCC 2 cut(s) 436, 511
SmiMI CAYNNNNRTG 5 cut(s) 555, 780, 942, 948, 1454
SmlI CTYRAG 1 cut(s) 1466
SmoI CTYRAG 1 cut(s) 1466
SspI AATATT 2 cut(s) 728, 1567
SspMI CTAG 3 cut(s) 36, 93, 1487
SstI GAGCTC 3 cut(s) 92, 840, 1373
StyD4I CCNGG 2 cut(s) 596, 1246
StyI CCWWGG 2 cut(s) 35, 1418
TaaI ACNGT 4 cut(s) 557, 651, 844, 1480
TaiI ACGT 2 cut(s) 532, 618
TaqI TCGA 5 cut(s) 287, 882, 974, 980, 1187
TatI WGTACW 2 cut(s) 245, 651
TfiI GAWTC 2 cut(s) 848, 996
Tru1I TTAA 3 cut(s) 630, 1334, 1410
Tru9I TTAA 3 cut(s) 630, 1334, 1410
TscAI CASTG 4 cut(s) 426, 588, 654, 847
TseFI GTSAC 2 cut(s) 1379, 1572
TseI GCWGC 5 cut(s) 251, 299, 1217, 1292, 1555
Tsp45I GTSAC 2 cut(s) 1379, 1572
TspRI CASTG 4 cut(s) 426, 588, 654, 847
Van91I CCANNNNNTGG 1 cut(s) 474
VpaK11BI GGWCC 2 cut(s) 436, 511
XapI RAATTY 5 cut(s) 626, 701, 825, 1087, 1183
XceI RCATGY 1 cut(s) 953
XcmI CCANNNNNNNNNTGG 1 cut(s) 505
XmaJI CCTAGG 1 cut(s) 35
XmiI GTMKAC 2 cut(s) 6, 618
XspI CTAG 3 cut(s) 36, 93, 1487
ZrmI AGTACT 1 cut(s) 247
Zsp2I ATGCAT 2 cut(s) 939, 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.