MD14G1074300.v1.1

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002314
Physical Location & Seq
Forward (+)
8278380 .. 8281051
2672 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1074300.v1.1.491

Sequence Viewer

Length: 1617 bp
ATGGCGCGGTATCAGCGGCACCAGCACCACCAGTGGAGAAAAGTTGAAAAGCCTTGTGCAGCCGCAGAGGAAGATGAAGAATCACAGACCACCACCTTTCTAATGGAGTTTGCAAACTCTGGTTACTATTACAAGAGAACAAGTCCCAAGCTTCTCTATCTCCTCTTCATCTCTTTCCTCTCTTGCAGCTTCATCTTAGCCCCTCATCTTTTCAGCTCCACCACTACTTTCTCTCTCCTTTATTCAACGCCTGTGGAAGATATGGATGTAAATGTTCCCTTGTGTTCTTCAATCTCTAACGGAACTATATGTTGCGATCGAAGCAATGTTCGTTCAGATATTTGTGTAATGAAAGGGGATGTAAGAACACACTCTGCTTCCTCCACTATCTTCCTCTACCGATCTACAGAAGGAAGTAGCTTCCAAAATCGTGTTTCTGATGTTGTTGAGGAAAATGAAGAAGATGAAACAGGGGAACTAGAGCATGAAAAGGTCAAACCGTATACACGGAAATGGGAAACAAGTGTCATGGACACCATTGATGAATTACAGCTCATTGCGAAGAAAGACACTCTAGGCATGCACCACCGGTGTGATGTGCAGCACGATGTTCCGGCAGTGTTTTTCTCAACCGGGGGATATACCGGTAATGTCTATCATGAATTTAATGATGGGATTATGCCATTGTTCATTACTTCCCAGCGGTTCAACAAGAAGGTTGTGTTTGTCATTCTCGATTACCATAATTGGTGGGTGACGAAATATGGTGACATTGTTTCTCAACTATCAGATTATCCGCCTATAGACTTTAGTGGAGATACAAGAACTCATTGCTTCCCAGAGGTCACAGTTGGTTTGAGGATTCACGACGAGCTCACTGTGGATTCTTCACTGATGGAGGGAAATGTGAGCATTGTTGACTTTCGAAATCTTCTAGACCGAGCCTACTGGCCTCGAATTAAAAGTCTTATTGAAGACGAGGAACGGGAAGCACAGGAAAAGCTTTCTGCATCTTTGGCATCTAAAAGCTCTTTCGAACCTGAGAGTGCAGTGCAAGAAGATCAGTTCAAGAAGCCTAAACTGGTCATCATATCTCGAAATGGATCAAGAGCGATAACAAATGAGAATTTAATGGTGAGAATGGCCGCGCAAATTGGGTTTGAAGTTAACGTTTTGAGACCTGACCGCACGACAGAGTTAGCAAAGATTTATCGGGCCCTTAATGCGAGTGATGTGATGATTGGGGTTCATGGTGCTGCCATGACACACTTTATGTTCATGAAGCCTGGGTCTGTGTTTATCCAAGTTGTTCCCCTTGGCACTGAGTGGGCAGCGAAGGAGTATTATGGGGAACCTGCAAGGAAGCTCGGCTTAAAATACATCGGCTACCAAATTCTAACTCGGGAGAGCTCGTTATACGCCAAATATGATAAGGATGATCCTGTTCTTAGGGATCCAGAGAGTGTAAACAAAATGGGATGGGAATACACAAAGAAAATCTATCTTGATGGCCAAACTGTGAGATTAGACCTCGGAAGATTTCGAAAGCGATTGGTTCGTGCTTACGGCTACATCTTCCACAGGTTGAACTATCACCCCCATCTTCAATCACGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

539

Amino Acids

61.94

Weight (kDa)

6.16

Isoelectric Point (pI)

34.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 349 - 434 5.4e-14 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1363
AccB1I GGYRCC 1 cut(s) 18
AccI GTMKAC 1 cut(s) 503
AccII CGCG 2 cut(s) 7, 1148
AciI CCGC 7 cut(s) 7, 16, 63, 703, 797, 1146, 1186
AclI AACGTT 1 cut(s) 1170
AclWI GGATC 4 cut(s) 1111, 1433, 1448, 1461
AcoI YGGCCR 2 cut(s) 1143, 1510
AcsI RAATTY 3 cut(s) 662, 1126, 1392
AdeI CACNNNGTG 1 cut(s) 1326
AgeI ACCGGT 2 cut(s) 588, 644
AgsI TTSAA 9 cut(s) 47, 246, 291, 709, 974, 1069, 1163, 1588, 1607
AjnI CCWGG 1 cut(s) 1285
AleI CACNNNNGTG 1 cut(s) 591
Alw21I GWGCWC 2 cut(s) 876, 1412
Alw26I GTCTC 1 cut(s) 1171
AlwI GGATC 4 cut(s) 1111, 1433, 1448, 1461
Ama87I CYCGRG 1 cut(s) 1401
AoxI GGCC 4 cut(s) 950, 1143, 1215, 1510
ApaI GGGCCC 1 cut(s) 1219
ApeKI GCWGC 5 cut(s) 59, 186, 601, 1256, 1331
ApoI RAATTY 3 cut(s) 662, 1126, 1392
ArsI GACNNNNNNTTYG 2 cut(s) 949, 981
AsiGI ACCGGT 2 cut(s) 588, 644
AspLEI GCGC 2 cut(s) 7, 1150
AspS9I GGNCC 2 cut(s) 1215, 1216
AsuC2I CCSGG 1 cut(s) 634
AsuHPI GGTGA 4 cut(s) 766, 779, 1147, 1586
AsuII TTCGAA 3 cut(s) 925, 1035, 1543
AvaI CYCGRG 1 cut(s) 1401
BaeGI GKGCMC 1 cut(s) 1219
BalI TGGCCA 1 cut(s) 1512
BamHI GGATCC 1 cut(s) 1453
BanI GGYRCC 1 cut(s) 18
BanII GRGCYC 3 cut(s) 876, 1219, 1412
BbsI GAAGAC 1 cut(s) 981
Bbv12I GWGCWC 2 cut(s) 876, 1412
BbvI GCAGC 5 cut(s) 71, 198, 613, 1243, 1343
BccI CCATC 5 cut(s) 665, 889, 1473, 1502, 1608
BceAI ACGGC 1 cut(s) 1582
BciT130I CCWGG 1 cut(s) 1287
BcnI CCSGG 1 cut(s) 634
BcoDI GTCTC 1 cut(s) 1171
BfaI CTAG 3 cut(s) 479, 575, 935
BfmI CTRYAG 2 cut(s) 405, 801
BfuAI ACCTGC 1 cut(s) 1363
BisI GCNGC 8 cut(s) 17, 60, 63, 187, 602, 1146, 1257, 1332
BlsI GCNGC 8 cut(s) 18, 61, 64, 188, 603, 1147, 1258, 1333
Bme1390I CCNGG 2 cut(s) 634, 1287
BmeT110I CYCGRG 1 cut(s) 1401
BmgT120I GGNCC 2 cut(s) 1215, 1216
BmiI GGNNCC 4 cut(s) 20, 1217, 1353, 1455
BmrFI CCNGG 2 cut(s) 634, 1287
BmsI GCATC 2 cut(s) 1019, 1028
BpiI GAAGAC 1 cut(s) 981
Bpu14I TTCGAA 3 cut(s) 925, 1035, 1543
BpuMI CCSGG 1 cut(s) 634
BsaI GGTCTC 1 cut(s) 1171
BsaJI CCNNGG 4 cut(s) 633, 1286, 1315, 1531
BsaWI WCCGGW 2 cut(s) 588, 644
BsaXI ACNNNNNCTCC 2 cut(s) 1397, 1427
Bse118I RCCGGY 2 cut(s) 588, 644
Bse1I ACTGG 3 cut(s) 31, 953, 1086
Bse3DI GCAATG 3 cut(s) 331, 555, 829
BseBI CCWGG 1 cut(s) 1287
BseDI CCNNGG 4 cut(s) 633, 1286, 1315, 1531
BseGI GGATG 4 cut(s) 271, 364, 1441, 1484
BseMI GCAATG 3 cut(s) 331, 555, 829
BseMII CTCAG 2 cut(s) 1032, 1314
BseNI ACTGG 3 cut(s) 31, 953, 1086
BseRI GAGGAG 1 cut(s) 152
BseSI GKGCMC 1 cut(s) 1219
BseXI GCAGC 5 cut(s) 71, 198, 613, 1243, 1343
BseYI CCCAGC 1 cut(s) 699
BsgI GTGCAG 3 cut(s) 78, 620, 1068
Bsh1236I CGCG 2 cut(s) 7, 1148
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 4 cut(s) 952, 1145, 1217, 1512
BshNI GGYRCC 1 cut(s) 18
BshTI ACCGGT 2 cut(s) 588, 644
BsiEI CGRYCG 1 cut(s) 319
BsiHKAI GWGCWC 2 cut(s) 876, 1412
BsiHKCI CYCGRG 1 cut(s) 1401
BsiSI CCGG 4 cut(s) 589, 614, 633, 645
BslFI GGGAC 1 cut(s) 129
BsmAI GTCTC 1 cut(s) 1171
BsmFI GGGAC 1 cut(s) 129
BsnI GGCC 4 cut(s) 952, 1145, 1217, 1512
Bso31I GGTCTC 1 cut(s) 1171
BsoBI CYCGRG 1 cut(s) 1401
Bsp119I TTCGAA 3 cut(s) 925, 1035, 1543
Bsp120I GGGCCC 1 cut(s) 1215
Bsp1286I GDGCHC 3 cut(s) 876, 1219, 1412
Bsp143I GATC 6 cut(s) 316, 401, 1060, 1103, 1438, 1453
BspACI CCGC 7 cut(s) 7, 16, 63, 703, 797, 1146, 1186
BspANI GGCC 4 cut(s) 952, 1145, 1217, 1512
BspCNI CTCAG 2 cut(s) 1033, 1315
BspFNI CGCG 2 cut(s) 7, 1148
BspHI TCATGA 2 cut(s) 658, 1278
BspLI GGNNCC 4 cut(s) 20, 1217, 1353, 1455
BspMI ACCTGC 1 cut(s) 1363
BspPI GGATC 4 cut(s) 1111, 1433, 1448, 1461
BspT104I TTCGAA 3 cut(s) 925, 1035, 1543
BspT107I GGYRCC 1 cut(s) 18
BspTNI GGTCTC 1 cut(s) 1171
BsrDI GCAATG 3 cut(s) 331, 555, 829
BsrFI RCCGGY 2 cut(s) 588, 644
BsrI ACTGG 3 cut(s) 31, 953, 1086
BssAI RCCGGY 2 cut(s) 588, 644
BssECI CCNNGG 4 cut(s) 633, 1286, 1315, 1531
BssMI GATC 6 cut(s) 316, 401, 1060, 1103, 1438, 1453
BssNAI GTATAC 1 cut(s) 504
BssT1I CCWWGG 1 cut(s) 1315
Bst1107I GTATAC 1 cut(s) 504
Bst2UI CCWGG 1 cut(s) 1287
Bst4CI ACNGT 5 cut(s) 501, 850, 880, 1519, 1614
Bst6I CTCTTC 1 cut(s) 170
BstBI TTCGAA 3 cut(s) 925, 1035, 1543
BstC8I GCNNGC 1 cut(s) 581
BstDEI CTNAG 4 cut(s) 196, 1041, 1323, 1448
BstF5I GGATG 4 cut(s) 271, 364, 1441, 1484
BstFNI CGCG 2 cut(s) 7, 1148
BstHHI GCGC 2 cut(s) 7, 1150
BstKTI GATC 6 cut(s) 319, 404, 1063, 1106, 1441, 1456
BstMAI GTCTC 1 cut(s) 1171
BstMBI GATC 6 cut(s) 316, 401, 1060, 1103, 1438, 1453
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 5 cut(s) 13, 22, 321, 1016, 1223
BstNI CCWGG 1 cut(s) 1287
BstNSI RCATGY 1 cut(s) 583
BstSCI CCNGG 2 cut(s) 632, 1285
BstSFI CTRYAG 2 cut(s) 405, 801
BstSLI GKGCMC 1 cut(s) 1219
BstUI CGCG 2 cut(s) 7, 1148
BstV1I GCAGC 5 cut(s) 71, 198, 613, 1243, 1343
BstV2I GAAGAC 1 cut(s) 981
BstX2I RGATCY 1 cut(s) 1453
BstYI RGATCY 1 cut(s) 1453
BstZ17I GTATAC 1 cut(s) 504
BsuRI GGCC 4 cut(s) 952, 1145, 1217, 1512
BtsCI GGATG 4 cut(s) 271, 364, 1441, 1484
BtsI GCAGTG 2 cut(s) 624, 1056
BtsIMutI CAGTG 6 cut(s) 38, 624, 876, 890, 1056, 1320
BveI ACCTGC 1 cut(s) 1363
Cac8I GCNNGC 1 cut(s) 581
CciI TCATGA 2 cut(s) 658, 1278
CfoI GCGC 2 cut(s) 7, 1150
Cfr10I RCCGGY 2 cut(s) 588, 644
Cfr13I GGNCC 2 cut(s) 1215, 1216
CspAI ACCGGT 2 cut(s) 588, 644
CspCI CAANNNNNGTGG 2 cut(s) 234, 269
CviAII CATG 7 cut(s) 485, 529, 580, 659, 1250, 1261, 1279
DdeI CTNAG 4 cut(s) 196, 1041, 1323, 1448
DpnI GATC 6 cut(s) 318, 403, 1062, 1105, 1440, 1455
DpnII GATC 6 cut(s) 316, 401, 1060, 1103, 1438, 1453
DraIII CACNNNGTG 1 cut(s) 1326
EaeI YGGCCR 2 cut(s) 1143, 1510
Eam1104I CTCTTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 170
EciI GGCGGA 1 cut(s) 786
Ecl136II GAGCTC 2 cut(s) 874, 1410
Eco130I CCWWGG 1 cut(s) 1315
Eco24I GRGCYC 3 cut(s) 876, 1219, 1412
Eco31I GGTCTC 1 cut(s) 1171
Eco53kI GAGCTC 2 cut(s) 874, 1410
Eco88I CYCGRG 1 cut(s) 1401
EcoICRI GAGCTC 2 cut(s) 874, 1410
EcoO109I RGGNCCY 1 cut(s) 1216
EcoRII CCWGG 1 cut(s) 1285
EcoT14I CCWWGG 1 cut(s) 1315
EcoT38I GRGCYC 3 cut(s) 876, 1219, 1412
ErhI CCWWGG 1 cut(s) 1315
FaeI CATG 7 cut(s) 488, 532, 583, 662, 1253, 1264, 1282
FalI AAGNNNNNCTT 2 cut(s) 1355, 1387
FaqI GGGAC 1 cut(s) 129
FatI CATG 7 cut(s) 484, 528, 579, 658, 1249, 1260, 1278
FblI GTMKAC 1 cut(s) 503
Fnu4HI GCNGC 8 cut(s) 17, 60, 63, 187, 602, 1146, 1257, 1332
FokI GGATG 4 cut(s) 278, 371, 1448, 1491
FriOI GRGCYC 3 cut(s) 876, 1219, 1412
Fsp4HI GCNGC 8 cut(s) 17, 60, 63, 187, 602, 1146, 1257, 1332
FspBI CTAG 3 cut(s) 479, 575, 935
GlaI GCGC 2 cut(s) 6, 1149
GluI GCNGC 8 cut(s) 17, 60, 63, 187, 602, 1146, 1257, 1332
GsaI CCCAGC 1 cut(s) 703
HaeIII GGCC 4 cut(s) 952, 1145, 1217, 1512
HapII CCGG 4 cut(s) 589, 614, 633, 645
HhaI GCGC 2 cut(s) 7, 1150
Hin1II CATG 7 cut(s) 488, 532, 583, 662, 1253, 1264, 1282
Hin6I GCGC 2 cut(s) 5, 1148
HinP1I GCGC 2 cut(s) 5, 1148
HincII GTYRAC 2 cut(s) 919, 1168
HindII GTYRAC 2 cut(s) 919, 1168
HindIII AAGCTT 2 cut(s) 149, 1001
HinfI GANTC 3 cut(s) 80, 862, 884
HpaI GTTAAC 1 cut(s) 1168
HpaII CCGG 4 cut(s) 589, 614, 633, 645
HphI GGTGA 4 cut(s) 766, 779, 1147, 1586
Hpy166II GTNNAC 4 cut(s) 504, 919, 1168, 1468
Hpy188I TCNGA 4 cut(s) 337, 439, 790, 1535
Hpy8I GTNNAC 4 cut(s) 504, 919, 1168, 1468
Hpy99I CGWCG 1 cut(s) 872
HpyAV CCTTC 3 cut(s) 404, 709, 1330
HpyCH4III ACNGT 5 cut(s) 501, 850, 880, 1519, 1614
HpyCH4IV ACGT 1 cut(s) 1170
HpyCH4V TGCA 9 cut(s) 59, 113, 186, 583, 601, 1010, 1049, 1054, 1358
HpyF10VI GCNNNNNNNGC 5 cut(s) 13, 22, 321, 1016, 1223
HpyF3I CTNAG 4 cut(s) 196, 1041, 1323, 1448
HpySE526I ACGT 1 cut(s) 1170
Hsp92II CATG 7 cut(s) 488, 532, 583, 662, 1253, 1264, 1282
HspAI GCGC 2 cut(s) 5, 1148
KspAI GTTAAC 1 cut(s) 1168
Kzo9I GATC 6 cut(s) 316, 401, 1060, 1103, 1438, 1453
LmnI GCTCC 1 cut(s) 221
Lsp1109I GCAGC 5 cut(s) 71, 198, 613, 1243, 1343
LweI GCATC 2 cut(s) 1019, 1028
MaeI CTAG 3 cut(s) 479, 575, 935
MaeII ACGT 1 cut(s) 1170
MaeIII GTNAC 4 cut(s) 122, 754, 767, 844
MalI GATC 6 cut(s) 318, 403, 1062, 1105, 1440, 1455
MboI GATC 6 cut(s) 316, 401, 1060, 1103, 1438, 1453
MflI RGATCY 1 cut(s) 1453
MhlI GDGCHC 3 cut(s) 876, 1219, 1412
MlsI TGGCCA 1 cut(s) 1512
MluCI AATT 7 cut(s) 545, 662, 745, 957, 1126, 1152, 1392
MluNI TGGCCA 1 cut(s) 1512
Mox20I TGGCCA 1 cut(s) 1512
MscI TGGCCA 1 cut(s) 1512
MseI TTAA 6 cut(s) 666, 960, 1130, 1167, 1221, 1373
MslI CAYNNNNRTG 2 cut(s) 511, 591
Msp20I TGGCCA 1 cut(s) 1512
MspA1I CMGCKG 2 cut(s) 16, 703
MspI CCGG 4 cut(s) 589, 614, 633, 645
MspR9I CCNGG 2 cut(s) 634, 1287
MvaI CCWGG 1 cut(s) 1287
MvnI CGCG 2 cut(s) 7, 1148
MwoI GCNNNNNNNGC 5 cut(s) 13, 22, 321, 1016, 1223
NciI CCSGG 1 cut(s) 634
NdeII GATC 6 cut(s) 316, 401, 1060, 1103, 1438, 1453
NlaIII CATG 7 cut(s) 488, 532, 583, 662, 1253, 1264, 1282
NlaIV GGNNCC 4 cut(s) 20, 1217, 1353, 1455
NmeAIII GCCGAG 1 cut(s) 1347
NmuCI GTSAC 3 cut(s) 754, 767, 844
NspI RCATGY 1 cut(s) 583
NspV TTCGAA 3 cut(s) 925, 1035, 1543
OliI CACNNNNGTG 1 cut(s) 591
PaeI GCATGC 1 cut(s) 583
PagI TCATGA 2 cut(s) 658, 1278
PfeI GAWTC 3 cut(s) 80, 862, 884
PinAI ACCGGT 2 cut(s) 588, 644
PkrI GCNGC 8 cut(s) 18, 61, 64, 188, 603, 1147, 1258, 1333
Ple19I CGATCG 1 cut(s) 319
Psp124BI GAGCTC 2 cut(s) 876, 1412
Psp1406I AACGTT 1 cut(s) 1170
Psp6I CCWGG 1 cut(s) 1285
PspFI CCCAGC 1 cut(s) 699
PspGI CCWGG 1 cut(s) 1285
PspN4I GGNNCC 4 cut(s) 20, 1217, 1353, 1455
PspOMI GGGCCC 1 cut(s) 1215
PspPI GGNCC 2 cut(s) 1215, 1216
PsuI RGATCY 1 cut(s) 1453
PvuI CGATCG 1 cut(s) 319
RseI CAYNNNNRTG 2 cut(s) 511, 591
SacI GAGCTC 2 cut(s) 876, 1412
SaqAI TTAA 6 cut(s) 666, 960, 1130, 1167, 1221, 1373
SatI GCNGC 8 cut(s) 17, 60, 63, 187, 602, 1146, 1257, 1332
Sau3AI GATC 6 cut(s) 316, 401, 1060, 1103, 1438, 1453
Sau96I GGNCC 2 cut(s) 1215, 1216
ScrFI CCNGG 2 cut(s) 634, 1287
SduI GDGCHC 3 cut(s) 876, 1219, 1412
SfaNI GCATC 2 cut(s) 1019, 1028
SfcI CTRYAG 2 cut(s) 405, 801
SfuI TTCGAA 3 cut(s) 925, 1035, 1543
SgrAI CRCCGGYG 1 cut(s) 588
SmiMI CAYNNNNRTG 2 cut(s) 511, 591
SphI GCATGC 1 cut(s) 583
Sse9I AATT 7 cut(s) 545, 662, 745, 957, 1126, 1152, 1392
SsiI CCGC 7 cut(s) 7, 16, 63, 703, 797, 1146, 1186
SspMI CTAG 3 cut(s) 479, 575, 935
SstI GAGCTC 2 cut(s) 876, 1412
StyD4I CCNGG 2 cut(s) 632, 1285
StyI CCWWGG 1 cut(s) 1315
TaaI ACNGT 5 cut(s) 501, 850, 880, 1519, 1614
TaiI ACGT 1 cut(s) 1173
TaqI TCGA 7 cut(s) 319, 735, 925, 955, 1035, 1096, 1543
TaqII GACCGA 1 cut(s) 954
TasI AATT 7 cut(s) 545, 662, 745, 957, 1126, 1152, 1392
TauI GCSGC 3 cut(s) 19, 65, 1148
TfiI GAWTC 3 cut(s) 80, 862, 884
Tru1I TTAA 6 cut(s) 666, 960, 1130, 1167, 1221, 1373
Tru9I TTAA 6 cut(s) 666, 960, 1130, 1167, 1221, 1373
TscAI CASTG 6 cut(s) 38, 624, 883, 897, 1056, 1327
TseFI GTSAC 3 cut(s) 754, 767, 844
TseI GCWGC 5 cut(s) 59, 186, 601, 1256, 1331
Tsp45I GTSAC 3 cut(s) 754, 767, 844
TspGWI ACGGA 2 cut(s) 315, 523
TspRI CASTG 6 cut(s) 38, 624, 883, 897, 1056, 1327
XapI RAATTY 3 cut(s) 662, 1126, 1392
XbaI TCTAGA 1 cut(s) 934
XceI RCATGY 1 cut(s) 583
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 503
XspI CTAG 3 cut(s) 479, 575, 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.