Rroxscaffold_6G00398530

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002314
Physical Location & Seq
Forward (+)
20382607 .. 20386222
3616 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00398530.1

Sequence Viewer

Length: 1560 bp
ATGGTATACCACCAGCGCAGGAAAGGCGAAAAGCCTACTGAGGATGAAGATGAGGAGGGTCAGAGTCTTGCAATGGAGTGTGCGAGCTCTGGTTACTACAAAAGATCAAGGCCCAAGCTTCTCACCTTTCTCTTCATCTCTCTTCTCTCTTGTAGCTTCATATTAGCCCCTCATCTGATATTTTCCTCTGTCACCACTTTCTCTCTTTCACAATCACCACAAGTAGAAAACGAAGGCCCTAGTATTGCTGCTGTTATAAACGCAAAGGCTCCCTTCTATTCTTCAGTCACTAATGGAGCTATATGTTGTGATAGAAGCAGTATCCGTTCAGATGTTTGTACCATGAAAGGGGATGTAAGAACGCACTCGCCTTCCTCTTCTATCTATGTCTACGAATCGCAAGACGAAGCAGTGAAGAATGAGGAGGATCTCCAACACGAAAAGATCAAACCATACACCAGAAAATGGGAAACAAATATTATGGACACCATTGATGAGTTGGACCTCATTGCAAAGAAAAACACTCTGATCATGAACCATCATAAATGTGATGTCCAACATGATGTTCCGGCAGTGTTTTTTTCAACAGGGGGCTACACCGGCAACGTCTACCATGAATTTAATGATGGGATAATGCCACTGTACATTACTTCCCAACATTTCAACAAGAAGGTTGTGTTCGTCATTCTTGAATTTCACAAATGGTGGGTCATGAAATATTCAGACATTCTTTCCCATCTCTCAGATTATCCTGTAATAAATTTCCATACTGATAAGAGAGTTCATTGCTTCCCTGAAGCCATTGTCGGTTTGAAAATTCACGATGAGCTGACTGTGGATTCTTCATTGATGGAAGGGAATGCGAGCATAGTCGAGTTTCGAAATCTCCTAGACAGAGCTTACCGGCCTCAGTTCAGAAGTCTAATGCATGAACATGCAAAGCTTTCTGTTGTTCCCAAGTTGAAAAAGCCATCAAGAATCAGGAGAAAAGTGCAAGAGCATCCATTCAACAAGCCGAAGCTGGTTATTATATCTCGAAATGGATCAAGGGCAATAACAAATGAGAAGTTGCTGGTGAAAATGGCTGAGAAAATCGGGTTTCAAGTCCAAGTTTTGAGGCCAGATAGCAGGACAATGTTAGCAGAGATTTATTGGGCTCTAAATTCGAGTGACGTCATGATTGGGGTTCATGGTGCTGCCATGACACATTTTCTGTTCATGAAGCCTGGTTCTGTGTTCATTCAAGTCATTCCTCTCGGAACTGAATGGGCAGCAGAGGCATATTATGGAGAACCTGCAAAGAAGCTTGGGTTGAACTACATTGGATACCAAATCCTTACCAAAGAGAGCTCATTATATGACAAGTATGATAAGGATGATCCTGTTCTTAAAGATCCAAGGAGTGTAACCAGAAAGGGATGGCAATACACAAAGACGATCTATCTTGAGGATCAGACAGTGAGACTACACCTCCCAAGATTTCGGAAGCAGTTGGTTGGCGCTTTTGATTACACCGTTGATAGATTGAATCACCAGAGTCCTAATATTCAGTCACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

519

Amino Acids

59.58

Weight (kDa)

8.57

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 314 - 414 1.9e-15 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 257
AatII GACGTC 1 cut(s) 1176
Acc36I ACCTGC 1 cut(s) 1303
AccB7I CCANNNNNTGG 1 cut(s) 465
AccI GTMKAC 3 cut(s) 6, 390, 609
AclWI GGATC 5 cut(s) 435, 1051, 1373, 1388, 1458
AcsI RAATTY 5 cut(s) 617, 692, 760, 816, 1162
AcuI CTGAAG 2 cut(s) 267, 816
AcyI GRCGYC 1 cut(s) 1173
AfaI GTAC 2 cut(s) 340, 644
AfiI CCNNNNNNNGG 2 cut(s) 348, 465
AjnI CCWGG 1 cut(s) 1225
AjuI GAANNNNNNNTTGG 2 cut(s) 549, 581
Alw21I GWGCWC 2 cut(s) 89, 1352
Alw26I GTCTC 1 cut(s) 1456
AlwI GGATC 5 cut(s) 435, 1051, 1373, 1388, 1458
AoxI GGCC 4 cut(s) 110, 235, 905, 1118
ApeKI GCWGC 3 cut(s) 248, 1196, 1271
ApoI RAATTY 5 cut(s) 617, 692, 760, 816, 1162
ArsI GACNNNNNNTTYG 2 cut(s) 693, 725
AspLEI GCGC 2 cut(s) 18, 1502
AspS9I GGNCC 3 cut(s) 111, 236, 502
AsuHPI GGTGA 5 cut(s) 115, 184, 207, 1087, 1523
AsuII TTCGAA 1 cut(s) 880
AvaII GGWCC 1 cut(s) 502
BanII GRGCYC 3 cut(s) 89, 1159, 1352
Bbv12I GWGCWC 2 cut(s) 89, 1352
BbvI GCAGC 3 cut(s) 235, 1183, 1283
BccI CCATC 6 cut(s) 546, 620, 744, 844, 979, 1413
BciT130I CCWGG 1 cut(s) 1227
BciVI GTATCC 2 cut(s) 332, 1319
BclI TGATCA 1 cut(s) 528
BcoDI GTCTC 1 cut(s) 1456
BfaI CTAG 2 cut(s) 240, 890
BfoI RGCGCY 1 cut(s) 1503
BfuAI ACCTGC 1 cut(s) 1303
BfuI GTATCC 2 cut(s) 332, 1319
BisI GCNGC 3 cut(s) 249, 1197, 1272
BlsI GCNGC 3 cut(s) 250, 1198, 1273
Bme1390I CCNGG 1 cut(s) 1227
Bme18I GGWCC 1 cut(s) 502
BmgT120I GGNCC 3 cut(s) 111, 236, 502
BmiI GGNNCC 1 cut(s) 270
BmrFI CCNGG 1 cut(s) 1227
BmsI GCATC 1 cut(s) 1009
Bpu14I TTCGAA 1 cut(s) 880
BpuEI CTTGAG 1 cut(s) 1466
BsaHI GRCGYC 1 cut(s) 1173
BsaJI CCNNGG 1 cut(s) 1397
BsaXI ACNNNNNCTCC 2 cut(s) 1455, 1485
Bsc4I CCNNNNNNNGG 2 cut(s) 348, 465
Bse118I RCCGGY 2 cut(s) 599, 903
Bse3DI GCAATG 3 cut(s) 78, 507, 784
BseBI CCWGG 1 cut(s) 1227
BseDI CCNNGG 1 cut(s) 1397
BseGI GGATG 5 cut(s) 49, 358, 1000, 1381, 1424
BseLI CCNNNNNNNGG 2 cut(s) 348, 465
BseMI GCAATG 3 cut(s) 78, 507, 784
BseMII CTCAG 4 cut(s) 30, 756, 923, 1077
BseRI GAGGAG 2 cut(s) 68, 437
BseXI GCAGC 3 cut(s) 235, 1183, 1283
BshFI GGCC 4 cut(s) 112, 237, 907, 1120
BsiHKAI GWGCWC 2 cut(s) 89, 1352
BsiSI CCGG 3 cut(s) 569, 600, 904
BslI CCNNNNNNNGG 2 cut(s) 348, 465
BsmAI GTCTC 1 cut(s) 1456
BsmI GAATGC 1 cut(s) 865
BsnI GGCC 4 cut(s) 112, 237, 907, 1120
Bsp119I TTCGAA 1 cut(s) 880
Bsp1286I GDGCHC 3 cut(s) 89, 1159, 1352
Bsp1407I TGTACA 1 cut(s) 642
Bsp143I GATC 9 cut(s) 104, 427, 444, 528, 1043, 1378, 1393, 1437, 1450
BspANI GGCC 4 cut(s) 112, 237, 907, 1120
BspCNI CTCAG 4 cut(s) 31, 755, 922, 1078
BspHI TCATGA 4 cut(s) 531, 711, 1176, 1218
BspLI GGNNCC 1 cut(s) 270
BspMI ACCTGC 1 cut(s) 1303
BspPI GGATC 5 cut(s) 435, 1051, 1373, 1388, 1458
BspT104I TTCGAA 1 cut(s) 880
BsrDI GCAATG 3 cut(s) 78, 507, 784
BsrFI RCCGGY 2 cut(s) 599, 903
BsrGI TGTACA 1 cut(s) 642
BssAI RCCGGY 2 cut(s) 599, 903
BssECI CCNNGG 1 cut(s) 1397
BssMI GATC 9 cut(s) 104, 427, 444, 528, 1043, 1378, 1393, 1437, 1450
BssNAI GTATAC 1 cut(s) 7
BssNI GRCGYC 1 cut(s) 1173
BssT1I CCWWGG 1 cut(s) 1397
Bst1107I GTATAC 1 cut(s) 7
Bst2UI CCWGG 1 cut(s) 1227
Bst4CI ACNGT 4 cut(s) 642, 835, 1459, 1516
Bst6I CTCTTC 3 cut(s) 137, 147, 382
BstACI GRCGYC 1 cut(s) 1173
BstAUI TGTACA 1 cut(s) 642
BstBI TTCGAA 1 cut(s) 880
BstC8I GCNNGC 2 cut(s) 85, 865
BstDEI CTNAG 4 cut(s) 39, 742, 909, 1086
BstF5I GGATG 5 cut(s) 49, 358, 1000, 1381, 1424
BstH2I RGCGCY 1 cut(s) 1503
BstHHI GCGC 2 cut(s) 18, 1502
BstKTI GATC 9 cut(s) 107, 430, 447, 531, 1046, 1381, 1396, 1440, 1453
BstMAI GTCTC 1 cut(s) 1456
BstMBI GATC 9 cut(s) 104, 427, 444, 528, 1043, 1378, 1393, 1437, 1450
BstMWI GCNNNNNNNGC 3 cut(s) 24, 600, 1277
BstNI CCWGG 1 cut(s) 1227
BstNSI RCATGY 1 cut(s) 938
BstSCI CCNGG 1 cut(s) 1225
BstV1I GCAGC 3 cut(s) 235, 1183, 1283
BstX2I RGATCY 2 cut(s) 427, 1393
BstYI RGATCY 2 cut(s) 427, 1393
BstZ17I GTATAC 1 cut(s) 7
BsuI GTATCC 2 cut(s) 332, 1319
BsuRI GGCC 4 cut(s) 112, 237, 907, 1120
BtsCI GGATG 5 cut(s) 49, 358, 1000, 1381, 1424
BtsI GCAGTG 2 cut(s) 417, 579
BtsIMutI CAGTG 4 cut(s) 417, 579, 638, 1464
BveI ACCTGC 1 cut(s) 1303
Cac8I GCNNGC 2 cut(s) 85, 865
CciI TCATGA 4 cut(s) 531, 711, 1176, 1218
CfoI GCGC 2 cut(s) 18, 1502
Cfr10I RCCGGY 2 cut(s) 599, 903
Cfr13I GGNCC 3 cut(s) 111, 236, 502
Csp6I GTAC 2 cut(s) 339, 643
CviQI GTAC 2 cut(s) 339, 643
DdeI CTNAG 4 cut(s) 39, 742, 909, 1086
DpnI GATC 9 cut(s) 106, 429, 446, 530, 1045, 1380, 1395, 1439, 1452
DpnII GATC 9 cut(s) 104, 427, 444, 528, 1043, 1378, 1393, 1437, 1450
Eam1104I CTCTTC 3 cut(s) 137, 147, 382
EarI CTCTTC 3 cut(s) 137, 147, 382
Ecl136II GAGCTC 2 cut(s) 87, 1350
Eco130I CCWWGG 1 cut(s) 1397
Eco24I GRGCYC 3 cut(s) 89, 1159, 1352
Eco47I GGWCC 1 cut(s) 502
Eco53kI GAGCTC 2 cut(s) 87, 1350
Eco57I CTGAAG 2 cut(s) 267, 816
EcoICRI GAGCTC 2 cut(s) 87, 1350
EcoO109I RGGNCCY 1 cut(s) 236
EcoRII CCWGG 1 cut(s) 1225
EcoT14I CCWWGG 1 cut(s) 1397
EcoT22I ATGCAT 1 cut(s) 930
EcoT38I GRGCYC 3 cut(s) 89, 1159, 1352
ErhI CCWWGG 1 cut(s) 1397
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FbaI TGATCA 1 cut(s) 528
FblI GTMKAC 3 cut(s) 6, 390, 609
Fnu4HI GCNGC 3 cut(s) 249, 1197, 1272
FokI GGATG 5 cut(s) 56, 365, 987, 1388, 1431
FriOI GRGCYC 3 cut(s) 89, 1159, 1352
Fsp4HI GCNGC 3 cut(s) 249, 1197, 1272
FspBI CTAG 2 cut(s) 240, 890
GlaI GCGC 2 cut(s) 17, 1501
GluI GCNGC 3 cut(s) 249, 1197, 1272
HaeII RGCGCY 1 cut(s) 1503
HaeIII GGCC 4 cut(s) 112, 237, 907, 1120
HapII CCGG 3 cut(s) 569, 600, 904
HhaI GCGC 2 cut(s) 18, 1502
Hin1I GRCGYC 1 cut(s) 1173
Hin6I GCGC 2 cut(s) 16, 1500
HinP1I GCGC 2 cut(s) 16, 1500
HindIII AAGCTT 3 cut(s) 116, 941, 1304
HinfI GANTC 6 cut(s) 64, 395, 839, 978, 1528, 1537
HpaII CCGG 3 cut(s) 569, 600, 904
HphI GGTGA 5 cut(s) 115, 184, 207, 1087, 1523
Hpy166II GTNNAC 3 cut(s) 7, 391, 610
Hpy8I GTNNAC 3 cut(s) 7, 391, 610
HpyAV CCTTC 5 cut(s) 227, 283, 381, 664, 848
HpyCH4III ACNGT 4 cut(s) 642, 835, 1459, 1516
HpyCH4IV ACGT 2 cut(s) 606, 1173
HpyCH4V TGCA 6 cut(s) 71, 512, 928, 938, 994, 1298
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 600, 1277
HpyF3I CTNAG 4 cut(s) 39, 742, 909, 1086
HpySE526I ACGT 2 cut(s) 606, 1173
Hsp92I GRCGYC 1 cut(s) 1173
HspAI GCGC 2 cut(s) 16, 1500
Ksp22I TGATCA 1 cut(s) 528
Kzo9I GATC 9 cut(s) 104, 427, 444, 528, 1043, 1378, 1393, 1437, 1450
LmnI GCTCC 2 cut(s) 274, 296
Lsp1109I GCAGC 3 cut(s) 235, 1183, 1283
LweI GCATC 1 cut(s) 1009
MaeI CTAG 2 cut(s) 240, 890
MaeII ACGT 2 cut(s) 606, 1173
MaeIII GTNAC 6 cut(s) 92, 190, 286, 1169, 1405, 1551
MalI GATC 9 cut(s) 106, 429, 446, 530, 1045, 1380, 1395, 1439, 1452
MboI GATC 9 cut(s) 104, 427, 444, 528, 1043, 1378, 1393, 1437, 1450
MboII GAAGA 7 cut(s) 59, 124, 134, 273, 369, 427, 834
MflI RGATCY 2 cut(s) 427, 1393
MhlI GDGCHC 3 cut(s) 89, 1159, 1352
MluCI AATT 5 cut(s) 617, 692, 760, 816, 1162
MlyI GAGTC 2 cut(s) 73, 1546
MmeI TCCRAC 3 cut(s) 457, 480, 580
Mph1103I ATGCAT 1 cut(s) 930
MseI TTAA 2 cut(s) 621, 1389
MslI CAYNNNNRTG 2 cut(s) 546, 933
MspI CCGG 3 cut(s) 569, 600, 904
MspR9I CCNGG 1 cut(s) 1227
Mva1269I GAATGC 1 cut(s) 865
MvaI CCWGG 1 cut(s) 1227
MwoI GCNNNNNNNGC 3 cut(s) 24, 600, 1277
NdeII GATC 9 cut(s) 104, 427, 444, 528, 1043, 1378, 1393, 1437, 1450
NlaIV GGNNCC 1 cut(s) 270
NmuCI GTSAC 4 cut(s) 190, 286, 1169, 1551
NsiI ATGCAT 1 cut(s) 930
NspI RCATGY 1 cut(s) 938
NspV TTCGAA 1 cut(s) 880
PagI TCATGA 4 cut(s) 531, 711, 1176, 1218
PctI GAATGC 1 cut(s) 865
PfeI GAWTC 4 cut(s) 395, 839, 978, 1528
PflMI CCANNNNNTGG 1 cut(s) 465
PkrI GCNGC 3 cut(s) 250, 1198, 1273
PleI GAGTC 2 cut(s) 72, 1545
PpsI GAGTC 2 cut(s) 72, 1545
PsiI TTATAA 1 cut(s) 257
Psp124BI GAGCTC 2 cut(s) 89, 1352
Psp6I CCWGG 1 cut(s) 1225
PspGI CCWGG 1 cut(s) 1225
PspN4I GGNNCC 1 cut(s) 270
PspPI GGNCC 3 cut(s) 111, 236, 502
PsuI RGATCY 2 cut(s) 427, 1393
RsaI GTAC 2 cut(s) 340, 644
RsaNI GTAC 2 cut(s) 339, 643
RseI CAYNNNNRTG 2 cut(s) 546, 933
SacI GAGCTC 2 cut(s) 89, 1352
SaqAI TTAA 2 cut(s) 621, 1389
SatI GCNGC 3 cut(s) 249, 1197, 1272
Sau3AI GATC 9 cut(s) 104, 427, 444, 528, 1043, 1378, 1393, 1437, 1450
Sau96I GGNCC 3 cut(s) 111, 236, 502
SchI GAGTC 2 cut(s) 73, 1546
ScrFI CCNGG 1 cut(s) 1227
SduI GDGCHC 3 cut(s) 89, 1159, 1352
SfaNI GCATC 1 cut(s) 1009
SfuI TTCGAA 1 cut(s) 880
SinI GGWCC 1 cut(s) 502
SmiMI CAYNNNNRTG 2 cut(s) 546, 933
SmlI CTYRAG 1 cut(s) 1445
SmoI CTYRAG 1 cut(s) 1445
Sse9I AATT 5 cut(s) 617, 692, 760, 816, 1162
SspI AATATT 3 cut(s) 478, 719, 1546
SspMI CTAG 2 cut(s) 240, 890
SstI GAGCTC 2 cut(s) 89, 1352
StyD4I CCNGG 1 cut(s) 1225
StyI CCWWGG 1 cut(s) 1397
TaaI ACNGT 4 cut(s) 642, 835, 1459, 1516
TaiI ACGT 2 cut(s) 609, 1176
TaqI TCGA 4 cut(s) 873, 880, 1036, 1166
TasI AATT 5 cut(s) 617, 692, 760, 816, 1162
TatI WGTACW 1 cut(s) 642
TfiI GAWTC 4 cut(s) 395, 839, 978, 1528
Tru1I TTAA 2 cut(s) 621, 1389
Tru9I TTAA 2 cut(s) 621, 1389
TscAI CASTG 4 cut(s) 417, 579, 645, 1464
TseFI GTSAC 4 cut(s) 190, 286, 1169, 1551
TseI GCWGC 3 cut(s) 248, 1196, 1271
Tsp45I GTSAC 4 cut(s) 190, 286, 1169, 1551
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 4 cut(s) 417, 579, 645, 1464
Van91I CCANNNNNTGG 1 cut(s) 465
VpaK11BI GGWCC 1 cut(s) 502
XapI RAATTY 5 cut(s) 617, 692, 760, 816, 1162
XceI RCATGY 1 cut(s) 938
XcmI CCANNNNNNNNNTGG 1 cut(s) 496
XmiI GTMKAC 3 cut(s) 6, 390, 609
XspI CTAG 2 cut(s) 240, 890
ZraI GACGTC 1 cut(s) 1174
Zsp2I ATGCAT 1 cut(s) 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.