MD12G1075400.v1.1

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002314
Physical Location & Seq
Forward (+)
9163197 .. 9167383
4187 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1075400.v1.1.491

Sequence Viewer

Length: 1590 bp
ATGGTATACCATCAGAGATACAAGCAGAGGAAAAAAGGTGAACATCTTACAGAAGAAGATGAAGAGTGTCAGAGCATTATCATGGGGTGTTCAAACTCTGGTTACTCCAAAAGAACAAGGCCCAAGCTTCTCTCTTTCGTCTTTCTCTCTCTCCTCTCTTGTAGCTTCATATTAGCACCTCATCTTTTCTACCCTAATGCCACTTTCTCTCTATTACGCCTTGCTGCTGATGATATGGTTGCATATGCTCCCTCGAGTTCTTCAATCTCTAACGGAACTATATTTTGTGACAGGAGCAGTTTTCGATCAGATGTTTGTATCCTGAAAGGGGATGTAAGAACACACTCCCGTTCCTCTTCCATCTTTGTCTACAGATCCAGAGATAAAAGTAATTTCACAAACTATCTATCGAGCATCGTCGAGGAAAACGAAGAGGAAAATGGGGAAAAGCTTCACCACGAAAAGATCAAACCGTATACCCGGAAATGGGAACCCAGTACCATGGACACCGTCACAGAATTAGACCTCATTGCAAAGACAGATGGCACTCCAGGAAAGCAACATGAGTGTGATGTCCAACATGAGGTTCCGGCCATATTCTTCTCAACGGCGGGCTATACCGGTAATGTTTATCATGAATTCAACGATGGTATTATGCCGTTGTACATTACTTCTCAGCATTTGAGGAAACAGGTTGTGTTTGTCATCGTTGATTTTCACAATTGGTGGCTTATGAAATACGGAGACATTCTTTCACAACTATCAGACTATCCAGTAATTAGTATCAGTGCAGACAAGAGAACTCATTGCTTTCCTGAAGCTACTGTCGGTCTGCAAATTCACGATGAGCTCACTGTGGATTCTTCAAATATGGAAGGAAATAAGAGCATATTCGACTTTCGAAATCTTCTAGACGGGGCTTACAGGCCTCGAGTTACAAGTCTGATTCACGAACGAGAAGCAAAAAAGAAACTCTCCGTTTCTATGTCTCCCGCATCGAAAAGATCCTTAAAAATCAAGAGAAAAGCACATGAAGCACATCAACTCAAGAGGGCTAGACTAGTGATCATATCTAGAAAAGGATCAAGAGCAATAACCAATGAGCATTTGCTGGTGAAAATGGCTGAAAAAATCGGGTTTGAAGTTGAAGTTCTGAGACCTAATTCGGGGACCGAGTTGGCAATGATTTATTGGGTTCTTAATTCGAGTGATGTCGTGATCGGGGTTCACGGTGCTGCCATGACACATTTTCTGTTCATGAGACCTGGCTCTGTGTTGATCCAAGTTATCCCTCTTGGAACTAGTTGGGCAGCAGAGGAATACTATGGGAAACCTGCAAGAAAGCTTGGGTTAAACTACATCGGCTACAAAATTCTTACCACAGAAAGCTCGTTACAGAACAAGTATGGTAAAGATGATCCTGTTCTTAAAAATCCAACTAGTGTAACAAAAAAAGGATGGGAATACACAAAGGAGATTTATCTTGAAGGTCAAACTGTGAGACTACACCTCACAAGATTTAAGAAGCAGTTGCTTCGTGCTTATGACTGCACCATTGGAAGAACGACTTGGCATTTCGAACAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

530

Amino Acids

60.49

Weight (kDa)

9.02

Isoelectric Point (pI)

40.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 336 - 427 6e-15 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1342
AccI GTMKAC 3 cut(s) 6, 369, 476
AciI CCGC 2 cut(s) 611, 993
AclWI GGATC 5 cut(s) 369, 999, 1090, 1273, 1412
AcoI YGGCCR 1 cut(s) 591
AcsI RAATTY 3 cut(s) 638, 837, 1371
AcuI CTGAAG 1 cut(s) 837
AfaI GTAC 2 cut(s) 499, 665
AfiI CCNNNNNNNGG 5 cut(s) 328, 486, 487, 583, 1166
AgeI ACCGGT 1 cut(s) 620
AgsI TTSAA 7 cut(s) 93, 264, 643, 867, 1142, 1148, 1487
AhlI ACTAGT 3 cut(s) 1060, 1301, 1439
AjnI CCWGG 2 cut(s) 550, 1264
AjuI GAANNNNNNNTTGG 4 cut(s) 570, 602, 1552, 1584
AluBI AGCT 7 cut(s) 127, 165, 451, 821, 850, 1345, 1389
AluI AGCT 7 cut(s) 127, 165, 451, 821, 850, 1345, 1389
Alw21I GWGCWC 1 cut(s) 852
Alw26I GTCTC 5 cut(s) 738, 993, 1150, 1255, 1495
AlwI GGATC 5 cut(s) 369, 999, 1090, 1273, 1412
Ama87I CYCGRG 2 cut(s) 253, 930
AoxI GGCC 3 cut(s) 119, 591, 926
ApeKI GCWGC 3 cut(s) 224, 1235, 1310
ApoI RAATTY 3 cut(s) 638, 837, 1371
AsiGI ACCGGT 1 cut(s) 620
Asp700I GAANNNNTTC 1 cut(s) 450
AspS9I GGNCC 2 cut(s) 120, 1170
AsuC2I CCSGG 1 cut(s) 481
AsuHPI GGTGA 3 cut(s) 50, 446, 1126
AsuII TTCGAA 2 cut(s) 901, 1578
AvaI CYCGRG 2 cut(s) 253, 930
AvaII GGWCC 1 cut(s) 1170
BanII GRGCYC 1 cut(s) 852
Bbv12I GWGCWC 1 cut(s) 852
BbvI GCAGC 3 cut(s) 211, 1222, 1322
BccI CCATC 5 cut(s) 18, 368, 536, 641, 1452
BceAI ACGGC 2 cut(s) 624, 643
BcgI CGANNNNNNTGC 2 cut(s) 1517, 1551
BciT130I CCWGG 2 cut(s) 552, 1266
BciVI GTATCC 1 cut(s) 329
BclI TGATCA 1 cut(s) 1065
BcnI CCSGG 1 cut(s) 481
BcoDI GTCTC 5 cut(s) 738, 993, 1150, 1255, 1495
BcuI ACTAGT 3 cut(s) 1060, 1301, 1439
BfaI CTAG 6 cut(s) 911, 1056, 1061, 1074, 1302, 1440
BfmI CTRYAG 1 cut(s) 370
BfuAI ACCTGC 1 cut(s) 1342
BfuI GTATCC 1 cut(s) 329
BisI GCNGC 3 cut(s) 225, 1236, 1311
BlsI GCNGC 3 cut(s) 226, 1237, 1312
Bme1390I CCNGG 3 cut(s) 481, 552, 1266
Bme18I GGWCC 1 cut(s) 1170
BmeT110I CYCGRG 2 cut(s) 253, 930
BmgT120I GGNCC 2 cut(s) 120, 1170
BmiI GGNNCC 3 cut(s) 492, 588, 1171
BmrFI CCNGG 3 cut(s) 481, 552, 1266
BmrI ACTGGG 1 cut(s) 489
BmsI GCATC 2 cut(s) 423, 1004
BmuI ACTGGG 1 cut(s) 489
BpmI CTGGAG 1 cut(s) 534
Bpu14I TTCGAA 2 cut(s) 901, 1578
BpuEI CTTGAG 1 cut(s) 1031
BpuMI CCSGG 1 cut(s) 481
BsaI GGTCTC 2 cut(s) 1150, 1255
BsaJI CCNNGG 1 cut(s) 501
BsaWI WCCGGW 1 cut(s) 620
Bsc4I CCNNNNNNNGG 5 cut(s) 328, 486, 487, 583, 1166
Bse118I RCCGGY 1 cut(s) 620
Bse1I ACTGG 2 cut(s) 495, 773
Bse3DI GCAATG 3 cut(s) 528, 805, 1188
BseBI CCWGG 2 cut(s) 552, 1266
BseDI CCNNGG 1 cut(s) 501
BseGI GGATG 2 cut(s) 337, 1463
BseLI CCNNNNNNNGG 5 cut(s) 328, 486, 487, 583, 1166
BseMI GCAATG 3 cut(s) 528, 805, 1188
BseMII CTCAG 2 cut(s) 689, 1145
BseNI ACTGG 2 cut(s) 495, 773
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 3 cut(s) 211, 1222, 1322
BsgI GTGCAG 2 cut(s) 810, 1534
BshFI GGCC 3 cut(s) 121, 593, 928
BshTI ACCGGT 1 cut(s) 620
BsiHKAI GWGCWC 1 cut(s) 852
BsiHKCI CYCGRG 2 cut(s) 253, 930
BsiSI CCGG 3 cut(s) 481, 590, 621
BslFI GGGAC 1 cut(s) 1183
BslI CCNNNNNNNGG 5 cut(s) 328, 486, 487, 583, 1166
BsmAI GTCTC 5 cut(s) 738, 993, 1150, 1255, 1495
BsmFI GGGAC 1 cut(s) 1183
BsnI GGCC 3 cut(s) 121, 593, 928
Bso31I GGTCTC 2 cut(s) 1150, 1255
BsoBI CYCGRG 2 cut(s) 253, 930
Bsp119I TTCGAA 2 cut(s) 901, 1578
Bsp1286I GDGCHC 1 cut(s) 852
Bsp1407I TGTACA 1 cut(s) 663
Bsp143I GATC 9 cut(s) 305, 374, 465, 1004, 1065, 1082, 1218, 1278, 1417
Bsp19I CCATGG 1 cut(s) 501
BspACI CCGC 2 cut(s) 611, 993
BspANI GGCC 3 cut(s) 121, 593, 928
BspCNI CTCAG 2 cut(s) 688, 1146
BspHI TCATGA 2 cut(s) 634, 1257
BspLI GGNNCC 3 cut(s) 492, 588, 1171
BspMI ACCTGC 1 cut(s) 1342
BspPI GGATC 5 cut(s) 369, 999, 1090, 1273, 1412
BspT104I TTCGAA 2 cut(s) 901, 1578
BspTNI GGTCTC 2 cut(s) 1150, 1255
BsrDI GCAATG 3 cut(s) 528, 805, 1188
BsrFI RCCGGY 1 cut(s) 620
BsrGI TGTACA 1 cut(s) 663
BsrI ACTGG 2 cut(s) 495, 773
BssAI RCCGGY 1 cut(s) 620
BssECI CCNNGG 1 cut(s) 501
BssMI GATC 9 cut(s) 305, 374, 465, 1004, 1065, 1082, 1218, 1278, 1417
BssNAI GTATAC 2 cut(s) 7, 477
BssT1I CCWWGG 1 cut(s) 501
Bst1107I GTATAC 2 cut(s) 7, 477
Bst2UI CCWGG 2 cut(s) 552, 1266
Bst4CI ACNGT 6 cut(s) 474, 511, 826, 856, 1232, 1498
Bst6I CTCTTC 3 cut(s) 57, 361, 426
BstAUI TGTACA 1 cut(s) 663
BstBI TTCGAA 2 cut(s) 901, 1578
BstC8I GCNNGC 1 cut(s) 613
BstDEI CTNAG 2 cut(s) 675, 1154
BstDSI CCRYGG 1 cut(s) 501
BstF5I GGATG 2 cut(s) 337, 1463
BstKTI GATC 9 cut(s) 308, 377, 468, 1007, 1068, 1085, 1221, 1281, 1420
BstMAI GTCTC 5 cut(s) 738, 993, 1150, 1255, 1495
BstMBI GATC 9 cut(s) 305, 374, 465, 1004, 1065, 1082, 1218, 1278, 1417
BstMWI GCNNNNNNNGC 1 cut(s) 1034
BstNI CCWGG 2 cut(s) 552, 1266
BstSCI CCNGG 3 cut(s) 479, 550, 1264
BstSFI CTRYAG 1 cut(s) 370
BstV1I GCAGC 3 cut(s) 211, 1222, 1322
BstX2I RGATCY 2 cut(s) 374, 1004
BstXI CCANNNNNNTGG 1 cut(s) 502
BstYI RGATCY 2 cut(s) 374, 1004
BstZ17I GTATAC 2 cut(s) 7, 477
BsuI GTATCC 1 cut(s) 329
BsuRI GGCC 3 cut(s) 121, 593, 928
BtgI CCRYGG 1 cut(s) 501
BtsCI GGATG 2 cut(s) 337, 1463
BtsIMutI CAGTG 2 cut(s) 793, 852
BveI ACCTGC 1 cut(s) 1342
Cac8I GCNNGC 1 cut(s) 613
CciI TCATGA 2 cut(s) 634, 1257
Cfr10I RCCGGY 1 cut(s) 620
Cfr13I GGNCC 2 cut(s) 120, 1170
Csp6I GTAC 2 cut(s) 498, 664
CspAI ACCGGT 1 cut(s) 620
CviAII CATG 8 cut(s) 82, 502, 563, 581, 635, 1031, 1240, 1258
CviQI GTAC 2 cut(s) 498, 664
DdeI CTNAG 2 cut(s) 675, 1154
DpnI GATC 9 cut(s) 307, 376, 467, 1006, 1067, 1084, 1220, 1280, 1419
DpnII GATC 9 cut(s) 305, 374, 465, 1004, 1065, 1082, 1218, 1278, 1417
EaeI YGGCCR 1 cut(s) 591
Eam1104I CTCTTC 3 cut(s) 57, 361, 426
EarI CTCTTC 3 cut(s) 57, 361, 426
Ecl136II GAGCTC 1 cut(s) 850
Eco130I CCWWGG 1 cut(s) 501
Eco147I AGGCCT 1 cut(s) 928
Eco24I GRGCYC 1 cut(s) 852
Eco31I GGTCTC 2 cut(s) 1150, 1255
Eco47I GGWCC 1 cut(s) 1170
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 1 cut(s) 837
Eco88I CYCGRG 2 cut(s) 253, 930
EcoICRI GAGCTC 1 cut(s) 850
EcoRI GAATTC 1 cut(s) 638
EcoRII CCWGG 2 cut(s) 550, 1264
EcoT14I CCWWGG 1 cut(s) 501
EcoT38I GRGCYC 1 cut(s) 852
ErhI CCWWGG 1 cut(s) 501
FaeI CATG 8 cut(s) 85, 505, 566, 584, 638, 1034, 1243, 1261
FalI AAGNNNNNCTT 2 cut(s) 1552, 1584
FaqI GGGAC 1 cut(s) 1183
FatI CATG 8 cut(s) 81, 501, 562, 580, 634, 1030, 1239, 1257
FauI CCCGC 2 cut(s) 604, 1000
FauNDI CATATG 1 cut(s) 244
FbaI TGATCA 1 cut(s) 1065
FblI GTMKAC 3 cut(s) 6, 369, 476
Fnu4HI GCNGC 3 cut(s) 225, 1236, 1311
FokI GGATG 2 cut(s) 344, 1470
FriOI GRGCYC 1 cut(s) 852
Fsp4HI GCNGC 3 cut(s) 225, 1236, 1311
FspBI CTAG 6 cut(s) 911, 1056, 1061, 1074, 1302, 1440
GluI GCNGC 3 cut(s) 225, 1236, 1311
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 3 cut(s) 121, 593, 928
HapII CCGG 3 cut(s) 481, 590, 621
Hin1II CATG 8 cut(s) 85, 505, 566, 584, 638, 1034, 1243, 1261
HindIII AAGCTT 3 cut(s) 125, 449, 1343
HinfI GANTC 2 cut(s) 860, 946
HpaII CCGG 3 cut(s) 481, 590, 621
HphI GGTGA 3 cut(s) 50, 446, 1126
Hpy166II GTNNAC 5 cut(s) 7, 41, 370, 477, 1228
Hpy188I TCNGA 6 cut(s) 15, 72, 310, 766, 945, 1155
Hpy8I GTNNAC 5 cut(s) 7, 41, 370, 477, 1228
Hpy99I CGWCG 1 cut(s) 422
HpyAV CCTTC 2 cut(s) 869, 1481
HpyCH4III ACNGT 6 cut(s) 474, 511, 826, 856, 1232, 1498
HpyCH4V TGCA 6 cut(s) 242, 533, 791, 835, 1337, 1551
HpyF10VI GCNNNNNNNGC 1 cut(s) 1034
HpyF3I CTNAG 2 cut(s) 675, 1154
Hsp92II CATG 8 cut(s) 85, 505, 566, 584, 638, 1034, 1243, 1261
Ksp22I TGATCA 1 cut(s) 1065
Kzo9I GATC 9 cut(s) 305, 374, 465, 1004, 1065, 1082, 1218, 1278, 1417
LmnI GCTCC 2 cut(s) 253, 294
Lsp1109I GCAGC 3 cut(s) 211, 1222, 1322
LweI GCATC 2 cut(s) 423, 1004
MaeI CTAG 6 cut(s) 911, 1056, 1061, 1074, 1302, 1440
MaeIII GTNAC 6 cut(s) 101, 287, 511, 934, 1392, 1444
MalI GATC 9 cut(s) 307, 376, 467, 1006, 1067, 1084, 1220, 1280, 1419
MboI GATC 9 cut(s) 305, 374, 465, 1004, 1065, 1082, 1218, 1278, 1417
MfeI CAATTG 1 cut(s) 721
MflI RGATCY 2 cut(s) 374, 1004
MhlI GDGCHC 1 cut(s) 852
MluCI AATT 9 cut(s) 391, 518, 638, 721, 777, 837, 1162, 1201, 1371
MmeI TCCRAC 2 cut(s) 601, 1460
MroXI GAANNNNTTC 1 cut(s) 450
MseI TTAA 5 cut(s) 1010, 1200, 1352, 1428, 1521
MslI CAYNNNNRTG 2 cut(s) 80, 567
MspI CCGG 3 cut(s) 481, 590, 621
MspR9I CCNGG 3 cut(s) 481, 552, 1266
MunI CAATTG 1 cut(s) 721
MvaI CCWGG 2 cut(s) 552, 1266
MwoI GCNNNNNNNGC 1 cut(s) 1034
NciI CCSGG 1 cut(s) 481
NcoI CCATGG 1 cut(s) 501
NdeI CATATG 1 cut(s) 244
NdeII GATC 9 cut(s) 305, 374, 465, 1004, 1065, 1082, 1218, 1278, 1417
NlaIII CATG 8 cut(s) 85, 505, 566, 584, 638, 1034, 1243, 1261
NlaIV GGNNCC 3 cut(s) 492, 588, 1171
NmuCI GTSAC 2 cut(s) 287, 511
NspV TTCGAA 2 cut(s) 901, 1578
PaeR7I CTCGAG 2 cut(s) 253, 930
PagI TCATGA 2 cut(s) 634, 1257
PceI AGGCCT 1 cut(s) 928
PcsI WCGNNNNNNNCGW 1 cut(s) 426
PdmI GAANNNNTTC 1 cut(s) 450
PfeI GAWTC 2 cut(s) 860, 946
PflFI GACNNNGTC 1 cut(s) 509
PfoI TCCNGGA 1 cut(s) 550
PinAI ACCGGT 1 cut(s) 620
PkrI GCNGC 3 cut(s) 226, 1237, 1312
Psp124BI GAGCTC 1 cut(s) 852
Psp6I CCWGG 2 cut(s) 550, 1264
PspGI CCWGG 2 cut(s) 550, 1264
PspN4I GGNNCC 3 cut(s) 492, 588, 1171
PspPI GGNCC 2 cut(s) 120, 1170
PspXI VCTCGAGB 2 cut(s) 253, 930
PsuI RGATCY 2 cut(s) 374, 1004
PsyI GACNNNGTC 1 cut(s) 509
RsaI GTAC 2 cut(s) 499, 665
RsaNI GTAC 2 cut(s) 498, 664
RseI CAYNNNNRTG 2 cut(s) 80, 567
SacI GAGCTC 1 cut(s) 852
SaqAI TTAA 5 cut(s) 1010, 1200, 1352, 1428, 1521
SatI GCNGC 3 cut(s) 225, 1236, 1311
Sau3AI GATC 9 cut(s) 305, 374, 465, 1004, 1065, 1082, 1218, 1278, 1417
Sau96I GGNCC 2 cut(s) 120, 1170
ScrFI CCNGG 3 cut(s) 481, 552, 1266
SduI GDGCHC 1 cut(s) 852
SfaNI GCATC 2 cut(s) 423, 1004
SfcI CTRYAG 1 cut(s) 370
Sfr274I CTCGAG 2 cut(s) 253, 930
SfuI TTCGAA 2 cut(s) 901, 1578
SinI GGWCC 1 cut(s) 1170
SlaI CTCGAG 2 cut(s) 253, 930
SmiMI CAYNNNNRTG 2 cut(s) 80, 567
SmlI CTYRAG 3 cut(s) 253, 930, 1046
SmoI CTYRAG 3 cut(s) 253, 930, 1046
SpeI ACTAGT 3 cut(s) 1060, 1301, 1439
Sse9I AATT 9 cut(s) 391, 518, 638, 721, 777, 837, 1162, 1201, 1371
SseBI AGGCCT 1 cut(s) 928
SsiI CCGC 2 cut(s) 611, 993
SspMI CTAG 6 cut(s) 911, 1056, 1061, 1074, 1302, 1440
SstI GAGCTC 1 cut(s) 852
StuI AGGCCT 1 cut(s) 928
StyD4I CCNGG 3 cut(s) 479, 550, 1264
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 6 cut(s) 474, 511, 826, 856, 1232, 1498
TaqII GACCGA 2 cut(s) 818, 1187
TasI AATT 9 cut(s) 391, 518, 638, 721, 777, 837, 1162, 1201, 1371
TatI WGTACW 1 cut(s) 663
TfiI GAWTC 2 cut(s) 860, 946
Tru1I TTAA 5 cut(s) 1010, 1200, 1352, 1428, 1521
Tru9I TTAA 5 cut(s) 1010, 1200, 1352, 1428, 1521
TscAI CASTG 2 cut(s) 793, 859
TseFI GTSAC 2 cut(s) 287, 511
TseI GCWGC 3 cut(s) 224, 1235, 1310
Tsp45I GTSAC 2 cut(s) 287, 511
TspDTI ATGAA 6 cut(s) 75, 157, 651, 749, 1047, 1246
TspGWI ACGGA 3 cut(s) 288, 756, 967
TspRI CASTG 2 cut(s) 793, 859
Tth111I GACNNNGTC 1 cut(s) 509
VpaK11BI GGWCC 1 cut(s) 1170
XapI RAATTY 3 cut(s) 638, 837, 1371
XbaI TCTAGA 2 cut(s) 910, 1073
XhoI CTCGAG 2 cut(s) 253, 930
XmiI GTMKAC 3 cut(s) 6, 369, 476
XmnI GAANNNNTTC 1 cut(s) 450
XspI CTAG 6 cut(s) 911, 1056, 1061, 1074, 1302, 1440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.