MD00G1122300.v1.1

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002314
Physical Location & Seq
Forward (+)
26215389 .. 26216120
732 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1122300.v1.1.491

Sequence Viewer

Length: 732 bp
ATGCCGTTGTTCATTACTTCCCAGCGTTTCAACAAGAAGGTTGTGTTTGTCATTCTCGAGTACCATAATTGGTGGGTGACGAAATATGGAGACATTGTTTCTCAACCATTAGATTATCAACCTATAGATTTTAGTGGAGATACAAGAACTCATTGCTTCCCTGAGGTCATCGTTGGTCTGAGGATTCACGACGAGCTCACTGTGGATTCCTCACTGATGGAGGGAAATGTGAGCATTGTTGACTTTCGAAATCTTCTAGACCGAGCCTACTGGCCTCGAATTAGAAGTCTTATTCAAGACGAGGAATGGGAAGCACTGGAGAAGTATTCTGCGTCTCTGGCATTTGAAAGCTCTTTCGAACCTGAGAGTGAAATGCAAGAAGATCAATTCAAGAAGCCAAAGCTGCTCATCATATCTCGAAATGGATCAAGAGCGATAACCAATGAGAATTTCTTAGTTAGAATGGCTGAGCAAATTGGGTTTGAAGTTAATGTTTTGAGACCTGACCGCAAGACAGAGTTAGCAAAGATTTATCGGGCCCTTAATGCGAGTGATGTCATGATCGGGGTTCATGGCGCTGCCATGACACATTTTATGTTCATGAGGCCTGGGTCTGTGTTCATCCAAGTTGTTCCCCTAGGAATTGAGTGGACAGCGGAGGAATACTATGGGGAACCTGCAAGGAAGCTCGGGTTGAAATACATCGGCATCCAAGTTTGTCTATTAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

244

Amino Acids

28.12

Weight (kDa)

5.35

Isoelectric Point (pI)

28.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 124 - 208 3.7e-14 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 685
AciI CCGC 2 cut(s) 508, 656
AclWI GGATC 1 cut(s) 433
AcsI RAATTY 1 cut(s) 448
AfaI GTAC 1 cut(s) 62
AgsI TTSAA 6 cut(s) 31, 296, 347, 391, 485, 697
AjnI CCWGG 1 cut(s) 607
AjuI GAANNNNNNNTTGG 2 cut(s) 434, 466
AluBI AGCT 4 cut(s) 196, 351, 403, 688
AluI AGCT 4 cut(s) 196, 351, 403, 688
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 3 cut(s) 84, 339, 493
AlwI GGATC 1 cut(s) 433
Ama87I CYCGRG 2 cut(s) 56, 689
AoxI GGCC 3 cut(s) 272, 537, 605
ApaI GGGCCC 1 cut(s) 541
ApeKI GCWGC 2 cut(s) 403, 578
ApoI RAATTY 1 cut(s) 448
ArsI GACNNNNNNTTYG 2 cut(s) 271, 303
AseI ATTAAT 1 cut(s) 725
AspA2I CCTAGG 1 cut(s) 637
AspLEI GCGC 1 cut(s) 578
AspS9I GGNCC 2 cut(s) 537, 538
AsuHPI GGTGA 1 cut(s) 88
AsuII TTCGAA 2 cut(s) 247, 357
AvaI CYCGRG 2 cut(s) 56, 689
AvrII CCTAGG 1 cut(s) 637
AxyI CCTNAGG 1 cut(s) 162
BaeGI GKGCMC 1 cut(s) 541
BanII GRGCYC 2 cut(s) 198, 541
Bbv12I GWGCWC 1 cut(s) 198
BbvI GCAGC 2 cut(s) 390, 565
BccI CCATC 1 cut(s) 211
BciT130I CCWGG 1 cut(s) 609
BcoDI GTCTC 3 cut(s) 84, 339, 493
BfaI CTAG 2 cut(s) 257, 638
BfmI CTRYAG 1 cut(s) 123
BfoI RGCGCY 1 cut(s) 579
BfuAI ACCTGC 1 cut(s) 685
BisI GCNGC 2 cut(s) 404, 579
BlnI CCTAGG 1 cut(s) 637
BlpI GCTNAGC 1 cut(s) 468
BlsI GCNGC 2 cut(s) 405, 580
Bme1390I CCNGG 1 cut(s) 609
BmeT110I CYCGRG 2 cut(s) 56, 689
BmgT120I GGNCC 2 cut(s) 537, 538
BmiI GGNNCC 2 cut(s) 539, 675
BmrFI CCNGG 1 cut(s) 609
BmsI GCATC 1 cut(s) 717
BpmI CTGGAG 1 cut(s) 338
Bpu1102I GCTNAGC 1 cut(s) 468
Bpu14I TTCGAA 2 cut(s) 247, 357
BsaI GGTCTC 1 cut(s) 493
BsaJI CCNNGG 2 cut(s) 608, 637
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bse1I ACTGG 2 cut(s) 275, 321
Bse21I CCTNAGG 1 cut(s) 162
Bse3DI GCAATG 1 cut(s) 151
BseBI CCWGG 1 cut(s) 609
BseDI CCNNGG 2 cut(s) 608, 637
BseGI GGATG 2 cut(s) 621, 708
BseMI GCAATG 1 cut(s) 151
BseMII CTCAG 4 cut(s) 153, 170, 354, 459
BseNI ACTGG 2 cut(s) 275, 321
BseSI GKGCMC 1 cut(s) 541
BseXI GCAGC 2 cut(s) 390, 565
BseYI CCCAGC 1 cut(s) 21
BshFI GGCC 3 cut(s) 274, 539, 607
BsiHKAI GWGCWC 1 cut(s) 198
BsiHKCI CYCGRG 2 cut(s) 56, 689
BsmAI GTCTC 3 cut(s) 84, 339, 493
BsmBI CGTCTC 1 cut(s) 339
BsnI GGCC 3 cut(s) 274, 539, 607
Bso31I GGTCTC 1 cut(s) 493
BsoBI CYCGRG 2 cut(s) 56, 689
Bsp119I TTCGAA 2 cut(s) 247, 357
Bsp120I GGGCCC 1 cut(s) 537
Bsp1286I GDGCHC 2 cut(s) 198, 541
Bsp143I GATC 3 cut(s) 382, 425, 561
Bsp1720I GCTNAGC 1 cut(s) 468
BspACI CCGC 2 cut(s) 508, 656
BspANI GGCC 3 cut(s) 274, 539, 607
BspCNI CTCAG 4 cut(s) 154, 171, 355, 460
BspHI TCATGA 2 cut(s) 558, 600
BspLI GGNNCC 2 cut(s) 539, 675
BspMI ACCTGC 1 cut(s) 685
BspPI GGATC 1 cut(s) 433
BspT104I TTCGAA 2 cut(s) 247, 357
BspTNI GGTCTC 1 cut(s) 493
BsrDI GCAATG 1 cut(s) 151
BsrI ACTGG 2 cut(s) 275, 321
BssECI CCNNGG 2 cut(s) 608, 637
BssMI GATC 3 cut(s) 382, 425, 561
BssT1I CCWWGG 1 cut(s) 637
Bst2UI CCWGG 1 cut(s) 609
Bst4CI ACNGT 1 cut(s) 202
BstBI TTCGAA 2 cut(s) 247, 357
BstDEI CTNAG 5 cut(s) 162, 179, 363, 454, 468
BstF5I GGATG 2 cut(s) 621, 708
BstH2I RGCGCY 1 cut(s) 579
BstHHI GCGC 1 cut(s) 578
BstKTI GATC 3 cut(s) 385, 428, 564
BstMAI GTCTC 3 cut(s) 84, 339, 493
BstMBI GATC 3 cut(s) 382, 425, 561
BstMWI GCNNNNNNNGC 3 cut(s) 338, 403, 545
BstNI CCWGG 1 cut(s) 609
BstSCI CCNGG 1 cut(s) 607
BstSFI CTRYAG 1 cut(s) 123
BstSLI GKGCMC 1 cut(s) 541
BstV1I GCAGC 2 cut(s) 390, 565
Bsu36I CCTNAGG 1 cut(s) 162
BsuRI GGCC 3 cut(s) 274, 539, 607
BtsCI GGATG 2 cut(s) 621, 708
BtsIMutI CAGTG 3 cut(s) 198, 212, 314
BveI ACCTGC 1 cut(s) 685
CciI TCATGA 2 cut(s) 558, 600
CfoI GCGC 1 cut(s) 578
Cfr13I GGNCC 2 cut(s) 537, 538
CseI GACGC 1 cut(s) 321
Csp6I GTAC 1 cut(s) 61
CviAII CATG 4 cut(s) 559, 572, 583, 601
CviQI GTAC 1 cut(s) 61
DdeI CTNAG 5 cut(s) 162, 179, 363, 454, 468
DpnI GATC 3 cut(s) 384, 427, 563
DpnII GATC 3 cut(s) 382, 425, 561
Ecl136II GAGCTC 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 637
Eco147I AGGCCT 1 cut(s) 607
Eco24I GRGCYC 2 cut(s) 198, 541
Eco31I GGTCTC 1 cut(s) 493
Eco53kI GAGCTC 1 cut(s) 196
Eco81I CCTNAGG 1 cut(s) 162
Eco88I CYCGRG 2 cut(s) 56, 689
EcoICRI GAGCTC 1 cut(s) 196
EcoO109I RGGNCCY 1 cut(s) 538
EcoRII CCWGG 1 cut(s) 607
EcoT14I CCWWGG 1 cut(s) 637
EcoT38I GRGCYC 2 cut(s) 198, 541
ErhI CCWWGG 1 cut(s) 637
Esp3I CGTCTC 1 cut(s) 339
FaeI CATG 4 cut(s) 562, 575, 586, 604
FatI CATG 4 cut(s) 558, 571, 582, 600
Fnu4HI GCNGC 2 cut(s) 404, 579
FokI GGATG 2 cut(s) 608, 695
FriOI GRGCYC 2 cut(s) 198, 541
Fsp4HI GCNGC 2 cut(s) 404, 579
FspBI CTAG 2 cut(s) 257, 638
GlaI GCGC 1 cut(s) 577
GluI GCNGC 2 cut(s) 404, 579
GsaI CCCAGC 1 cut(s) 25
GsuI CTGGAG 1 cut(s) 338
HaeII RGCGCY 1 cut(s) 579
HaeIII GGCC 3 cut(s) 274, 539, 607
HgaI GACGC 1 cut(s) 321
HhaI GCGC 1 cut(s) 578
Hin1II CATG 4 cut(s) 562, 575, 586, 604
Hin6I GCGC 1 cut(s) 576
HinP1I GCGC 1 cut(s) 576
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 2 cut(s) 184, 206
HphI GGTGA 1 cut(s) 88
Hpy166II GTNNAC 2 cut(s) 241, 651
Hpy188I TCNGA 1 cut(s) 180
Hpy188III TCNNGA 9 cut(s) 56, 188, 257, 296, 391, 417, 429, 559, 601
Hpy8I GTNNAC 2 cut(s) 241, 651
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 1 cut(s) 31
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4V TGCA 2 cut(s) 376, 680
HpyF10VI GCNNNNNNNGC 3 cut(s) 338, 403, 545
HpyF3I CTNAG 5 cut(s) 162, 179, 363, 454, 468
Hsp92II CATG 4 cut(s) 562, 575, 586, 604
HspAI GCGC 1 cut(s) 576
Kzo9I GATC 3 cut(s) 382, 425, 561
Lsp1109I GCAGC 2 cut(s) 390, 565
LweI GCATC 1 cut(s) 717
MaeI CTAG 2 cut(s) 257, 638
MaeIII GTNAC 1 cut(s) 76
MalI GATC 3 cut(s) 384, 427, 563
MboI GATC 3 cut(s) 382, 425, 561
MboII GAAGA 2 cut(s) 245, 392
MhlI GDGCHC 2 cut(s) 198, 541
MluCI AATT 6 cut(s) 67, 279, 386, 448, 474, 642
MnlI CCTC 8 cut(s) 157, 174, 214, 220, 285, 295, 597, 652
MseI TTAA 3 cut(s) 489, 543, 725
MspA1I CMGCKG 1 cut(s) 656
MspR9I CCNGG 1 cut(s) 609
MvaI CCWGG 1 cut(s) 609
MwoI GCNNNNNNNGC 3 cut(s) 338, 403, 545
NdeII GATC 3 cut(s) 382, 425, 561
NlaIII CATG 4 cut(s) 562, 575, 586, 604
NlaIV GGNNCC 2 cut(s) 539, 675
NmuCI GTSAC 1 cut(s) 76
NspV TTCGAA 2 cut(s) 247, 357
PaeR7I CTCGAG 1 cut(s) 56
PagI TCATGA 2 cut(s) 558, 600
PceI AGGCCT 1 cut(s) 607
PfeI GAWTC 2 cut(s) 184, 206
PkrI GCNGC 2 cut(s) 405, 580
PshBI ATTAAT 1 cut(s) 725
Psp124BI GAGCTC 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 607
PspFI CCCAGC 1 cut(s) 21
PspGI CCWGG 1 cut(s) 607
PspN4I GGNNCC 2 cut(s) 539, 675
PspOMI GGGCCC 1 cut(s) 537
PspPI GGNCC 2 cut(s) 537, 538
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
SacI GAGCTC 1 cut(s) 198
SaqAI TTAA 3 cut(s) 489, 543, 725
SatI GCNGC 2 cut(s) 404, 579
Sau3AI GATC 3 cut(s) 382, 425, 561
Sau96I GGNCC 2 cut(s) 537, 538
ScrFI CCNGG 1 cut(s) 609
SduI GDGCHC 2 cut(s) 198, 541
SfaNI GCATC 1 cut(s) 717
SfcI CTRYAG 1 cut(s) 123
Sfr274I CTCGAG 1 cut(s) 56
SfuI TTCGAA 2 cut(s) 247, 357
SlaI CTCGAG 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
Sse9I AATT 6 cut(s) 67, 279, 386, 448, 474, 642
SseBI AGGCCT 1 cut(s) 607
SsiI CCGC 2 cut(s) 508, 656
SspMI CTAG 2 cut(s) 257, 638
SstI GAGCTC 1 cut(s) 198
StuI AGGCCT 1 cut(s) 607
StyD4I CCNGG 1 cut(s) 607
StyI CCWWGG 1 cut(s) 637
TaaI ACNGT 1 cut(s) 202
TaqI TCGA 5 cut(s) 57, 247, 277, 357, 418
TaqII GACCGA 1 cut(s) 276
TasI AATT 6 cut(s) 67, 279, 386, 448, 474, 642
TfiI GAWTC 2 cut(s) 184, 206
Tru1I TTAA 3 cut(s) 489, 543, 725
Tru9I TTAA 3 cut(s) 489, 543, 725
TscAI CASTG 3 cut(s) 205, 219, 321
TseFI GTSAC 1 cut(s) 76
TseI GCWGC 2 cut(s) 403, 578
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 3 cut(s) 560, 589, 610
TspRI CASTG 3 cut(s) 205, 219, 321
VspI ATTAAT 1 cut(s) 725
XapI RAATTY 1 cut(s) 448
XbaI TCTAGA 1 cut(s) 256
XhoI CTCGAG 1 cut(s) 56
XmaJI CCTAGG 1 cut(s) 637
XspI CTAG 2 cut(s) 257, 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.