RLG00000023299

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002314
Physical Location & Seq
Forward (+)
21376594 .. 21378744
2151 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023299

Sequence Viewer

Length: 1608 bp
ATGGCGCGCTATCAGCGGTGTCATCAGTGGAAAAAAGGTGAAAAGCATATAGACGAAGATGAAGAGTCTCAGACGTTTCTGATGGAGTTTGCAAACTCTGGTTATTACAAAAGATCAAGTCCCAAGCTTCTCTATCTTCTCTTCATCTCTCTTCTCTCTTGCAGCTTCATATTAGCCCCTCACCTTTTCAGTTCCAGCACCACTTTCTCTCTCTTATATTCAACTCTGATAGAAGATGAAGGGCTTGCTGGTGATATGAATATAAATGCTCCCCTGTGTTCTTCGATCTCTAATGGAGCTATATGTTGTGATCGTAGCAGTATTCGTTCAGATATTTGTATCATGAAAGGGGATGTGAGAACCCAGTCTACTTCCTTTACGATCTTTGTCTATGGATCTGAAGACAGAAGTAGCTCCAAAAGTCTGTTTTCAGGTATTGTTGAGGAAAATGAGGAACTACAACACGAAAGGATCAAACCATACACCCGGAAATGGGAAACTAGTGTGATGAACACCATTGATGAATTACACCTCATTGCAAAGAAAGACAGTACTCTAGACCTGTACCAACAACAGTGTGATGTCCAACATGATGTTCCAGCTGTGTTTTTCTCGACTGGGGGCTATACCGGTAATGTTTATCATGAATTCAATGATGGGATTCTGCCATTGTACATTACCTCCCAGCATTTCGACAAAAAGGTTGTGTTTGTCATTCAGGATTATCATAATTGGTGGGTCATGAAATATGGAGACATTCTTTCACAGCTATCAGATTACCCACCTATAGACTTCAGTGGAGATAAGAGAACTCATTGCTTCCCTGAGGCCATTGTTGGTCTGAGAATTCATGACGAGCTTTCTGTTGATTCTTCACTGATGGAGAGGAATAAAGACATTGTTGATTTCCGGGATCTCCTAGACAGAGCATACTGGCCTCGAATTAGAGGTTTAATTCAAGATGAGGAACGAGAAGCGCAGGAGAAGCTTTCAGTGTCTCCCACATTTGAAAGCTCCTTAGATATCAAAAGAGAAGTGCACGAAGATCAACTTTTGAAGAAACCTAAACTGCTAATCATATCTCGGAATGGATCAAGAGCGATAACTAATGAAAATTTATTGGTGAAGATGGCTGAGAAAATCGGTTTTGAAGTTGAGGTTTTGAGACCAGACCGCACGACAGAGTTAGCAAAGATTTATCGGGCACTTAATGCGAGTGATGTCATGATTGGAGTTCATGGTGCTGCTATGACTCATTTTCTCTTCATGAAGCCTCACTCTGTTTTCATCCAAGTTATTCCTCTCGGAACTGAATGGGCAGCAGAAGAGTATTATGGAGAACCTGCAAAGAAGCTTGGCTTGAAATACATTGGCTATCAAATTCTCGCAAGAGAGAGCTCTTTGTATGGCAAATATGATAGAGATGACCCTGTTCTTCGAGATCCACAGAGCGTAAACAAAAAGGGATGGGAATACACAAAGAAGATCTATCTTGATGGCCAAACTGTGAGATTAGACCTTGGGAGATTTCGGAAACGTTTGGTTCGTGCTTATGATTACATGTTGAGTAGAATGAACCAGCGTCCCCATCTGCAATATTCACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

536

Amino Acids

61.89

Weight (kDa)

6.09

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 344 - 430 4e-14 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1351
AccI GTMKAC 1 cut(s) 368
AccII CGCG 1 cut(s) 7
AciI CCGC 2 cut(s) 16, 1174
AclI AACGTT 1 cut(s) 1537
AclWI GGATC 5 cut(s) 403, 479, 921, 1099, 1436
AcoI YGGCCR 1 cut(s) 1498
AcsI RAATTY 4 cut(s) 647, 846, 1114, 1380
AcuI CTGAAG 2 cut(s) 420, 778
AfaI GTAC 3 cut(s) 553, 566, 674
AfiI CCNNNNNNNGG 2 cut(s) 492, 493
AflIII ACRYGT 1 cut(s) 1560
AgeI ACCGGT 1 cut(s) 629
AgsI TTSAA 7 cut(s) 222, 652, 959, 1010, 1057, 1151, 1363
AhlI ACTAGT 1 cut(s) 500
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
Alw21I GWGCWC 2 cut(s) 1041, 1400
Alw26I GTCTC 4 cut(s) 72, 747, 1002, 1159
Alw44I GTGCAC 1 cut(s) 1037
AlwI GGATC 5 cut(s) 403, 479, 921, 1099, 1436
AoxI GGCC 3 cut(s) 828, 935, 1498
ApaLI GTGCAC 1 cut(s) 1037
ApeKI GCWGC 3 cut(s) 162, 1244, 1319
ApoI RAATTY 4 cut(s) 647, 846, 1114, 1380
AsiGI ACCGGT 1 cut(s) 629
AspLEI GCGC 3 cut(s) 7, 9, 979
AsuC2I CCSGG 2 cut(s) 487, 911
AsuHPI GGTGA 4 cut(s) 50, 173, 263, 1135
AxyI CCTNAGG 1 cut(s) 825
BaeGI GKGCMC 2 cut(s) 1041, 1207
BalI TGGCCA 1 cut(s) 1500
BanII GRGCYC 1 cut(s) 1400
BbsI GAAGAC 1 cut(s) 408
Bbv12I GWGCWC 2 cut(s) 1041, 1400
BbvI GCAGC 3 cut(s) 174, 1231, 1331
BccI CCATC 7 cut(s) 76, 650, 874, 1123, 1461, 1490, 1596
BcnI CCSGG 2 cut(s) 487, 911
BcoDI GTCTC 4 cut(s) 72, 747, 1002, 1159
BcuI ACTAGT 1 cut(s) 500
BfaI CTAG 3 cut(s) 501, 557, 920
BfmI CTRYAG 1 cut(s) 786
BfuAI ACCTGC 1 cut(s) 1351
BglII AGATCT 1 cut(s) 1485
BisI GCNGC 3 cut(s) 163, 1245, 1320
BlsI GCNGC 3 cut(s) 164, 1246, 1321
BmcAI AGTACT 1 cut(s) 553
Bme1390I CCNGG 2 cut(s) 487, 911
BmrFI CCNGG 2 cut(s) 487, 911
BmrI ACTGGG 2 cut(s) 358, 627
BmuI ACTGGG 2 cut(s) 358, 627
BpiI GAAGAC 1 cut(s) 408
BpuMI CCSGG 2 cut(s) 487, 911
BsaI GGTCTC 1 cut(s) 1159
BsaJI CCNNGG 1 cut(s) 1519
BsaWI WCCGGW 1 cut(s) 629
BsaXI ACNNNNNCTCC 2 cut(s) 665, 695
Bsc4I CCNNNNNNNGG 2 cut(s) 492, 493
Bse118I RCCGGY 1 cut(s) 629
Bse1I ACTGG 3 cut(s) 364, 622, 938
Bse21I CCTNAGG 1 cut(s) 825
Bse3DI GCAATG 2 cut(s) 534, 814
BseDI CCNNGG 1 cut(s) 1519
BseGI GGATG 3 cut(s) 358, 1287, 1472
BseLI CCNNNNNNNGG 2 cut(s) 492, 493
BseMI GCAATG 2 cut(s) 534, 814
BseMII CTCAG 4 cut(s) 83, 816, 833, 1125
BseNI ACTGG 3 cut(s) 364, 622, 938
BsePI GCGCGC 1 cut(s) 5
BseSI GKGCMC 2 cut(s) 1041, 1207
BseXI GCAGC 3 cut(s) 174, 1231, 1331
BseYI CCCAGC 1 cut(s) 684
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 3 cut(s) 830, 937, 1500
BshTI ACCGGT 1 cut(s) 629
BsiHKAI GWGCWC 2 cut(s) 1041, 1400
BsiSI CCGG 3 cut(s) 487, 630, 910
BslFI GGGAC 2 cut(s) 105, 1569
BslI CCNNNNNNNGG 2 cut(s) 492, 493
BsmAI GTCTC 4 cut(s) 72, 747, 1002, 1159
BsmFI GGGAC 2 cut(s) 105, 1569
BsnI GGCC 3 cut(s) 830, 937, 1500
Bso31I GGTCTC 1 cut(s) 1159
Bsp1286I GDGCHC 3 cut(s) 1041, 1207, 1400
Bsp1407I TGTACA 1 cut(s) 672
BspACI CCGC 2 cut(s) 16, 1174
BspANI GGCC 3 cut(s) 830, 937, 1500
BspCNI CTCAG 4 cut(s) 82, 817, 834, 1126
BspFNI CGCG 1 cut(s) 7
BspHI TCATGA 6 cut(s) 342, 643, 741, 850, 1224, 1266
BspMI ACCTGC 1 cut(s) 1351
BspPI GGATC 5 cut(s) 403, 479, 921, 1099, 1436
BspTNI GGTCTC 1 cut(s) 1159
BsrDI GCAATG 2 cut(s) 534, 814
BsrFI RCCGGY 1 cut(s) 629
BsrGI TGTACA 1 cut(s) 672
BsrI ACTGG 3 cut(s) 364, 622, 938
BssAI RCCGGY 1 cut(s) 629
BssECI CCNNGG 1 cut(s) 1519
BssHII GCGCGC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 1519
Bst4CI ACNGT 4 cut(s) 551, 576, 1507, 1605
Bst6I CTCTTC 5 cut(s) 57, 146, 156, 1268, 1320
BstAPI GCANNNNNTGC 1 cut(s) 1211
BstAUI TGTACA 1 cut(s) 672
BstC8I GCNNGC 2 cut(s) 7, 246
BstDEI CTNAG 5 cut(s) 69, 825, 842, 1018, 1134
BstF5I GGATG 3 cut(s) 358, 1287, 1472
BstFNI CGCG 1 cut(s) 7
BstHHI GCGC 3 cut(s) 7, 9, 979
BstMAI GTCTC 4 cut(s) 72, 747, 1002, 1159
BstMWI GCNNNNNNNGC 3 cut(s) 13, 985, 1211
BstNSI RCATGY 1 cut(s) 1564
BstSCI CCNGG 2 cut(s) 485, 909
BstSFI CTRYAG 1 cut(s) 786
BstSLI GKGCMC 2 cut(s) 1041, 1207
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 3 cut(s) 174, 1231, 1331
BstV2I GAAGAC 1 cut(s) 408
BstX2I RGATCY 4 cut(s) 395, 913, 1441, 1485
BstYI RGATCY 4 cut(s) 395, 913, 1441, 1485
Bsu36I CCTNAGG 1 cut(s) 825
BsuRI GGCC 3 cut(s) 830, 937, 1500
BtsCI GGATG 3 cut(s) 358, 1287, 1472
BtsIMutI CAGTG 5 cut(s) 32, 581, 802, 875, 999
BveI ACCTGC 1 cut(s) 1351
Cac8I GCNNGC 2 cut(s) 7, 246
CciI TCATGA 6 cut(s) 342, 643, 741, 850, 1224, 1266
CfoI GCGC 3 cut(s) 7, 9, 979
Cfr10I RCCGGY 1 cut(s) 629
CseI GACGC 1 cut(s) 1571
Csp6I GTAC 3 cut(s) 552, 565, 673
CspAI ACCGGT 1 cut(s) 629
CviAII CATG 9 cut(s) 343, 590, 644, 742, 851, 1225, 1238, 1267, 1561
CviQI GTAC 3 cut(s) 552, 565, 673
DdeI CTNAG 5 cut(s) 69, 825, 842, 1018, 1134
EaeI YGGCCR 1 cut(s) 1498
Eam1104I CTCTTC 5 cut(s) 57, 146, 156, 1268, 1320
EarI CTCTTC 5 cut(s) 57, 146, 156, 1268, 1320
Ecl136II GAGCTC 1 cut(s) 1398
Eco130I CCWWGG 1 cut(s) 1519
Eco24I GRGCYC 1 cut(s) 1400
Eco31I GGTCTC 1 cut(s) 1159
Eco32I GATATC 1 cut(s) 1024
Eco53kI GAGCTC 1 cut(s) 1398
Eco57I CTGAAG 2 cut(s) 420, 778
Eco81I CCTNAGG 1 cut(s) 825
EcoICRI GAGCTC 1 cut(s) 1398
EcoRI GAATTC 2 cut(s) 647, 846
EcoRV GATATC 1 cut(s) 1024
EcoT14I CCWWGG 1 cut(s) 1519
EcoT38I GRGCYC 1 cut(s) 1400
ErhI CCWWGG 1 cut(s) 1519
FaeI CATG 9 cut(s) 346, 593, 647, 745, 854, 1228, 1241, 1270, 1564
FalI AAGNNNNNCTT 4 cut(s) 1035, 1067, 1343, 1375
FaqI GGGAC 2 cut(s) 105, 1569
FatI CATG 9 cut(s) 342, 589, 643, 741, 850, 1224, 1237, 1266, 1560
FblI GTMKAC 1 cut(s) 368
Fnu4HI GCNGC 3 cut(s) 163, 1245, 1320
FokI GGATG 3 cut(s) 365, 1274, 1479
FriOI GRGCYC 1 cut(s) 1400
Fsp4HI GCNGC 3 cut(s) 163, 1245, 1320
FspBI CTAG 3 cut(s) 501, 557, 920
GlaI GCGC 3 cut(s) 6, 8, 978
GluI GCNGC 3 cut(s) 163, 1245, 1320
GsaI CCCAGC 1 cut(s) 688
HaeIII GGCC 3 cut(s) 830, 937, 1500
HapII CCGG 3 cut(s) 487, 630, 910
HgaI GACGC 1 cut(s) 1571
HhaI GCGC 3 cut(s) 7, 9, 979
Hin1II CATG 9 cut(s) 346, 593, 647, 745, 854, 1228, 1241, 1270, 1564
Hin6I GCGC 3 cut(s) 5, 7, 977
HinP1I GCGC 3 cut(s) 5, 7, 977
HindIII AAGCTT 3 cut(s) 125, 986, 1352
HinfI GANTC 4 cut(s) 65, 661, 869, 1252
HpaII CCGG 3 cut(s) 487, 630, 910
HphI GGTGA 4 cut(s) 50, 173, 263, 1135
Hpy166II GTNNAC 3 cut(s) 369, 1039, 1456
Hpy8I GTNNAC 3 cut(s) 369, 1039, 1456
HpyAV CCTTC 1 cut(s) 233
HpyCH4III ACNGT 4 cut(s) 551, 576, 1507, 1605
HpyCH4IV ACGT 2 cut(s) 74, 1537
HpyCH4V TGCA 6 cut(s) 92, 162, 539, 1039, 1346, 1594
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 985, 1211
HpyF3I CTNAG 5 cut(s) 69, 825, 842, 1018, 1134
HpySE526I ACGT 2 cut(s) 74, 1537
Hsp92II CATG 9 cut(s) 346, 593, 647, 745, 854, 1228, 1241, 1270, 1564
HspAI GCGC 3 cut(s) 5, 7, 977
LmnI GCTCC 4 cut(s) 274, 296, 419, 1019
Lsp1109I GCAGC 3 cut(s) 174, 1231, 1331
MaeI CTAG 3 cut(s) 501, 557, 920
MaeII ACGT 2 cut(s) 74, 1537
MflI RGATCY 4 cut(s) 395, 913, 1441, 1485
MhlI GDGCHC 3 cut(s) 1041, 1207, 1400
MlsI TGGCCA 1 cut(s) 1500
MluCI AATT 8 cut(s) 524, 647, 730, 846, 942, 954, 1114, 1380
MluNI TGGCCA 1 cut(s) 1500
MlyI GAGTC 2 cut(s) 74, 1246
MmeI TCCRAC 1 cut(s) 610
Mox20I TGGCCA 1 cut(s) 1500
MscI TGGCCA 1 cut(s) 1500
MseI TTAA 2 cut(s) 953, 1209
Msp20I TGGCCA 1 cut(s) 1500
MspA1I CMGCKG 2 cut(s) 16, 602
MspI CCGG 3 cut(s) 487, 630, 910
MspR9I CCNGG 2 cut(s) 487, 911
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 3 cut(s) 13, 985, 1211
NciI CCSGG 2 cut(s) 487, 911
NlaIII CATG 9 cut(s) 346, 593, 647, 745, 854, 1228, 1241, 1270, 1564
NspI RCATGY 1 cut(s) 1564
PagI TCATGA 6 cut(s) 342, 643, 741, 850, 1224, 1266
PauI GCGCGC 1 cut(s) 5
PciI ACATGT 1 cut(s) 1560
PcsI WCGNNNNNNNCGW 1 cut(s) 1543
PfeI GAWTC 2 cut(s) 661, 869
PfoI TCCNGGA 1 cut(s) 909
PinAI ACCGGT 1 cut(s) 629
PkrI GCNGC 3 cut(s) 164, 1246, 1321
PleI GAGTC 2 cut(s) 73, 1246
PpsI GAGTC 2 cut(s) 73, 1246
PscI ACATGT 1 cut(s) 1560
Psp124BI GAGCTC 1 cut(s) 1400
Psp1406I AACGTT 1 cut(s) 1537
PspFI CCCAGC 1 cut(s) 684
PsrI GAACNNNNNNTAC 2 cut(s) 352, 384
PsuI RGATCY 4 cut(s) 395, 913, 1441, 1485
PteI GCGCGC 1 cut(s) 5
PvuII CAGCTG 1 cut(s) 602
RsaI GTAC 3 cut(s) 553, 566, 674
RsaNI GTAC 3 cut(s) 552, 565, 673
SacI GAGCTC 1 cut(s) 1400
SaqAI TTAA 2 cut(s) 953, 1209
SatI GCNGC 3 cut(s) 163, 1245, 1320
ScaI AGTACT 1 cut(s) 553
SchI GAGTC 2 cut(s) 74, 1246
ScrFI CCNGG 2 cut(s) 487, 911
SduI GDGCHC 3 cut(s) 1041, 1207, 1400
SfcI CTRYAG 1 cut(s) 786
SpeI ACTAGT 1 cut(s) 500
Sse9I AATT 8 cut(s) 524, 647, 730, 846, 942, 954, 1114, 1380
SsiI CCGC 2 cut(s) 16, 1174
SspI AATATT 1 cut(s) 1598
SspMI CTAG 3 cut(s) 501, 557, 920
SstI GAGCTC 1 cut(s) 1400
StyD4I CCNGG 2 cut(s) 485, 909
StyI CCWWGG 1 cut(s) 1519
TaaI ACNGT 4 cut(s) 551, 576, 1507, 1605
TaiI ACGT 2 cut(s) 77, 1540
TaqI TCGA 5 cut(s) 284, 614, 693, 940, 1438
TasI AATT 8 cut(s) 524, 647, 730, 846, 942, 954, 1114, 1380
TatI WGTACW 2 cut(s) 551, 672
TfiI GAWTC 2 cut(s) 661, 869
Tru1I TTAA 2 cut(s) 953, 1209
Tru9I TTAA 2 cut(s) 953, 1209
TscAI CASTG 5 cut(s) 32, 581, 802, 882, 999
TseI GCWGC 3 cut(s) 162, 1244, 1319
TspRI CASTG 5 cut(s) 32, 581, 802, 882, 999
VneI GTGCAC 1 cut(s) 1037
XapI RAATTY 4 cut(s) 647, 846, 1114, 1380
XbaI TCTAGA 1 cut(s) 556
XceI RCATGY 1 cut(s) 1564
XmiI GTMKAC 1 cut(s) 368
XspI CTAG 3 cut(s) 501, 557, 920
ZrmI AGTACT 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.